Two-dimensional electrophoresis performed on a microfluidic chip could reduce the consumption of sample and reagents, realizing high-throughput analysis in a very short time. During the isoelectric focusing ( IEF) separation, the buffer for the two different separation methods should be kept from being mixed. After the IEF, the protein was transferred from the IEF channel into the second dimension channels. Based on the pseudo valve structure, this paper reported a method to deposit a titanium dioxide membrane at the interface of two dimensional channels, which enhanced the performance of the pseudo valve. The IEF electrophoresis and SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) procedures were characterized respectively. The total separation took 10-15 min, and the protein's migration rate was linear with the migration time, and inversely proportional to the logarithm of protein molecular weight. Based on the above results, the differential two-dimensional electrophoresis was performed to test the deviation between different measurements.

Objective:To study the thickness distribution palatal bone in adolescents and provide a clinical reference for implanting mini-screws.Methods:Cone-beam CT head scan data of palate of 20 adolescent orthodontic patients were measured with NNT Viewer software.60 points of the palate bone on one side were designed as 1 -10 in sagittal direction and as A -F in transvers direction with incisive foramen as the point of A and 0,the distance between each 2 adjacent points was 2 mm.The bone thickness of the 60 points were measured and statistically analyzed.Results:There were no significant differences among B2,B3,C2,C3,D3,E3,E4,F4 and F5(P >0.05).Statistically significant difference was found between F3 and B2 or B3,C2,C3,D3,E3,E4,F4 and F5(P ＜0.05).There was significant difference between D3 and D4(P ＜0.05).Conclusion:The appropriate areas for implanting mini-screws in palate may be about 2 -4 mm near the palatal suture and 4 -6 mm behind incisor canal,and 8 -10 mm near the palatal su-ture,6 -8 mm behind incisor canal.Those regions are safe for implanting mini-screws and can provide about 6 mm bone tissue sup-port.

Seven hundred and forty-six mice, weighing 30-35 grams, were divided into four groups randomly. The animals of the first three groups were inflicted with radiation injury (12 Gy total body irradiation from a Co60 source) , burn injury (15% of third degree burns), and combined injury of radiation and burns(12 Gy total body irradiation and 15% of third degree burns) respectively. The fourth group worked as normal control.The mortality and the dynamic changes of the intestinal epithelium of the experimental animals were studied. It was found that the clinical course of the combined injury group was quite different from other two. The mortality of the combined injury group was higher and the damage on the intestinal epithelium was more severe than those of the radiation injury group in the first 48 hours after injury. In the second 48 hours, the mortality of the combined injury group became lower and the recovery of the intestinal epithelium faster.The reactions of the experimental animals to the combined injury of radiation and burn were-very complicate. Further investigation is essential to elucidate the mechanism and to formulate appropriate therapeutic measures.

OBJECTIVE:To improve package inserts of chemical drugs so as to promote safe,rational and effective use of chemical drugs.METHODS:A total of 558 package inserts of different chemical drugs commonly used in the clinic were collected from our hospital and other local hospitals and analyzed statistically based on the regulations stipulated by the "Norms of Package inserts of Drugs(trial)".RESULTS:Some important items such as pharmaco-toxicology,usage and dosage,pharmacokinetics etc in the package inserts of chemical drugs failed to meet the state standards,but the package inserts of the imported chemical drugs superior to those of the domestic ones,those of the injections superior to those of oral preparations,and those of oral preparations superior to those of external preparations.CONCLUSION:The package inserts of chemical drugs are far from perfect,which thus remain to be improved further.

OBJECTIVE:To investigate the drugs that labeled with the warning remarks for pregnant and lactant women for reference of patients' rational drug use.METHODS:The package inserts of drugs commonly used in our hospital were investigated and the drugs with warming remarks for pregnant and lactant women were collected.RESULTS:A total of 186 package inserts were found to have warning remarks for pregnant and lactant women.CONCLUSION:Pharmacists should attach great importance to the rational use of drugs for pregnant and lactant women in drug dispending and tighten control on the prescriptions with contraindication in prescription check,and they should cooperate with clinicians in the rational use and choice of drugs based on package inserts.

Objective To investigate the clinical significance of the treatment of intrahepatic bile duct stones with fiber choledochoscope combined with intraoperative ultrasound.Methods The clinical data of 185 cases with intrahepatic bile duct stone were analysed retrospectively,included 96 cases with stone removal by fiber choledochoscope intra-and post-operatively(choledochoscope group),and 89 cases with stone removal by fiber choledochoscope combined with intraoperative ultrasound(combined group).Results The rateo of bile duct retained stones in choledochoscope group(9.38%) was higher than that in combined group(2.08%)(P=0.041),and the rate of bile duct recurrent stones in choledochoscope group(12.34%) was higher than that in combined group(3.61%)(P=0.036).There was no significant differences between the two groups in the occarrence of cholangitis(P=0.087),but 3 cases of severe cholangitis required re-operation in choledochoscope group.Conclusions Fiber choledochoscope combined with intraoperative ultrasound can decrease the rate of bile duct retained stones and bile duct recurrent stones,and can improve the therapeutic effect.

Objective:To explore a scheduling method of rehabilitation medical resource for smart traditional Chinese medicine(TCM) for dysphagia because of cerebral apoplexy in order to save diagnosis time for patient and reasonably arrange treatment process for medical personnel.Methods: We designed the framework of smart TCM rehabilitation system, and proposed the medical resource scheduling model including acupuncture, massage and rehabilitation training. In addition, the genetic algorithm was employed to establish the scheduling method under the optimal objective towards the scheduling time.Results: (1) The treatment time of five dysphagia patients by using rehabilitation resource scheduling in Beijing Zhongguancun Hospital were saved 42.5% from the total treatment time compared to without scheduling; (2)The rehabilitation process of twenty virtual dysphagia patients were treated by the simulation scheduling, and 71% of total treatment time was saved. The efficiency of diagnosis and treatment was improved obviously .Conclusion: Smart TCM rehabilitation resource scheduling method can be used in an assisted rehabilitation therapy for dysphagia because of cerebral apoplexy, and it can improve the efficiency of diagnosis and treatment for patient and save a lot of medical resources.

The changes of PAF activity and the effects of anisodamine (654-2) were studied in the superior mesenteric artery occlusion (SMAO)shock in rabbits. The results showed that plasma PAF activity increased from 3.88?0.82 AU/ml before SM-AO to 9.71?1.26 AU/ml (P

Objective Study on the best drug compatibility for the double-lumen endobronchial intubation anesthesia. Methods Eighty ASA Ⅰ - Ⅱ patients undergoing selective thoracis surgery requiring intubation with double-lumen tubes were randomly divided into A and B group, with 40 cases in each group.The systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), were recorded before induction (T0) , after drug injection (T1), during intubation (T2), and at 1 min (T3), 3 min (T4)and 5 min (T5) after intubation. Results There was no significant difference in SBP, DBP and HR between the two groups at T0[(124. 9 ± 16. 0) mmHg vs (125.8 ±6. 4) mmHg, (73. 1 ±9. 9)mmHg vs (74. 3± 10. 4) mmHg, (81.8 ± 6. 6) times/min vs (82. 4 ± 8. 1) times/min] (P ＞ 0. 05). Compared with parameter at T0, SBP, DBP and HR, parameters in two groups in T1 were all significantly decreased after anesthesia [(94. 8 ± 10. 03) mmHg vs (96. 9 ± 10. 1) mmHg, (57. 3 ± 7. 66) mmHg vs (55.4 ± 7. 03) mmHg,(69. 6 ± 7. 43) times/min vs (66. 3 ± 7. 03) times/min] (P ＜ 0. 05). The cardiovascular parameters at T0,T2, T3, T4 were all comparable with those in group B [SBP: (130 ± 11.6) mmHg, (125.6 ± 10. 43) mmHg,(120. 1 ± 12. 3)mmHg,(116. 8± 11.4)mmHg;DBP:(75.6 ±9. 12)mmHg,(76. 2 ±9. 8)mmHg,(73. 1 ±9. 2) mmHg, (71.6 ± 8.46) mmHg; HR: (88 ± 9. 12) times/min, (82. 9 ± 7.5) times/min, (81.9 ± 8.2)times/min, (79. 9 ± 7. 8) times/min] (P ＞ 0. 05) , which were significantly higher than those in group A [SBP: (146. 3 ± 14. 2) mmHg, (141.2 ± 10. 63) mmHg, (137. 2 ± 13.23) mmHg, (122. 9 ± 11.6) mmHg;DBP: (94. 9 ± 10. 6) mmHg, (84 ± 9.63) mmHg, (79. 9 ± 9) mmHg, (75.8 ± 8. 3) mmHg; HR: (102 ±10. 63) times/min, (97.6 ± 9. 23) times/min, (87. 7 ± 8. 2) times/min, (82. 1 ± 7.32) times/min] (P ＜0. 05). The parameters at T2, T3, T4 in group A were obviously higher than those group B (P ＜ 0. 05).Conclusions Cardiovascular response with double-lumen endobronchial intubation by sufentanil-induced was stronger than fentaty, sufentanil had more stable hemodynamic parameters and it worth to be usd in clinic.

Objective:To evaluate the relationship between single nucleotide polymorphism (SNP) +45T→G in adiponectin gene exon 2 and type 2 diabetes mellitus (T2DM), obesity and insulin resistance (IR) in Han population in Guangxi. Methods:Polymer-ase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) was performed to identify the T-G polymorphism of adiponec-tin (exon 2) in 313 unrelated Han people from Guangxi including 95 normal glucose tolerance,103 impaired glucose tolerance and 115 type 2 diabetes mellitus. Results:In Han people of Guangxi, the SNP +45 GG genotype in adiponectin gene had significantly lower HDL-C levels than TT and TG genotypes in Han people(P<0. 05). The subjects with the GG genotype at position 45 had a signifi-cantly increased risk of IR (odds ratio of 2. 876[95%CI:1. 141-7. 248]) compared with those with the TT genotype at position 45. Conclusion:In Han people of Guangxi, the SNP +45 TT genotype in adiponectin gene has significantly higher HDL-C levels than GG genotype. The subjects with the GG genotype at position 45 have a significantly increased risk of IR compared with those with the TT genotype, while obesity and T2DM are independent on SNP+45 of adiponectin gene.