2.Streptococcal toxic shock syndrome: report of 2 cases.
Lin DONG ; Shi-Jun HE ; Ya-Li ZHANG
Chinese Journal of Pediatrics 2007;45(4):306-307
3.Rheumatic fever reappraised.
Chinese Medical Journal 2005;118(5):360-361
4.Study on the epidemiologic characteristics of human Streptococcus suis infection in Hechi city, Guangxi.
Ge HUANG ; Jian-Ming LI ; Da-Hao HUANG
Chinese Journal of Epidemiology 2008;29(2):207-208
Adult
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Aged
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China
;
epidemiology
;
Female
;
Humans
;
Male
;
Middle Aged
;
Streptococcal Infections
;
epidemiology
;
microbiology
;
Streptococcus suis
;
pathogenicity
7.MLST typing of Streptococcus suis isolated from clinical patients in Guangdong Province in 2005.
Hong-min WANG ; Chang-wen KE ; Wu-bin PAN ; Bi-xia KE ; Jing-diao CHEN ; Xiao-ling DENG ; Mei-zhen LIU ; Guo-ren CHEN ; Xing-fen YANG ; Zhen-yu ZHU
Journal of Southern Medical University 2008;28(8):1438-1441
Intensive surveillance of human S.suis infection was carried out in July and August of 2005 in Guangdong Province, which coincided with the Sichuan outbreak. Five isolated cases of human infections were identified during this period, from which 5 S. suis serotype 2 isolates were recovered. MLST analysis showed that these 5 isolates shared identical sequences of 6 MLST housekeeping genes except for one point mutation found within the thrA gene fragment, a neutral mutation (TTA to TTG) in the third nucleotide (360 nt) of the codon for leucine. MLST analysis identified 2 sequence types in the Guangdong sporadic infection. Three Guangdong isolates L-SS002, L-SS003 and L-SS005 belonged to ST7, while the other two isolates L-SS004 and L-SS006 belonged to ST1, but they all belonged to ST1 clonal complex. This finding represents a striking feature that differs from the Sichuan outbreak caused by a single ST7 SS2 clone. The 3 isolates of ST7 were probably imported from Sichuan Province, while the origin of the other 2 isolates of ST1 still remain to be clarified.
Animals
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Bacterial Typing Techniques
;
methods
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China
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DNA, Bacterial
;
genetics
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Humans
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Sequence Analysis, DNA
;
Streptococcal Infections
;
microbiology
;
Streptococcus suis
;
classification
;
genetics
;
pathogenicity
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Swine
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Swine Diseases
;
microbiology
;
Zoonoses
;
microbiology
8.Horizontal transmission of Streptococcus mutans in caries-active preschool children.
Danyang HU ; Wei CUI ; Yanping LUO ; Jiyong YANG ; Bin DENG ; Juan XU ; Bingfeng CHU ; Chenglong WANG
Journal of Southern Medical University 2014;34(5):636-640
OBJECTIVETo analyze horizontal transmission patterns of Streptococcus mutans among caries-active preschool children for early interventions of dental caries.
METHODSPlaque samples obtained from 20 caries-active preschool children between 4 and 5 years of age were cultured under anaerobic conditions for isolating S. mutans, which were identified by morphological and biochemical analyses and PCR using primers homologous to the surface protein glucosyltransferase B (gtfB). The genotypes of the isolated S. mutans strains were determined by arbitrarily primed PCR (AP-PCR).
RESULTSOf the 200 S. mutans isolates obtained, 19 were excluded by biochemical analysis, and the remaining 181 isolates were identified as S. mutans by PCR with primers of gtfB, showing 37 different genotypes as identified by AP-PCR. Six children were found to carry S. mutans of a single genotype, 11 carried 2 genotypes, 2 had 3 genotypes, and 1 had 4 genotypes; 2 children from different classes were found to carry S. mutans of the same single genotype.
CONCLUSIONWe identified 37 genotypes of S. mutans in these caries-active preschool children, among whom horizontal transmissions of the strains were not found.
Child, Preschool ; Dental Caries ; microbiology ; Dental Plaque ; Genotype ; Glucosyltransferases ; Humans ; Polymerase Chain Reaction ; Streptococcal Infections ; transmission ; Streptococcus mutans ; classification
9.Comparison of M-serotypes of Streptococcus pyogenes isolated from healthy elementary school children in two rural areas.
Seon Ju KIM ; Eui Chong KIM ; Sung Ho CHA ; Edward L KAPLAN
Journal of Korean Medical Science 1996;11(2):133-136
Serotypings have been used as markers for epidemiological surveys of Streptococcus pyogenes infections. Seventy-seven strains of S. pyogenes isolated from the throats of elementary school children in Kangwon Province in Korea in March and April 1992 were serotyped with M and/or opacity factor (OF) antisera. Sixty-eight strains of S. pyogenes from healthy school children in Chungnam Province in March 1993 were also serotyped and the distribution of serotype was compared with the isolates from those living in Kangwon Province. The distributions of M types were quite different between the two areas. M-78 (46.8%) and M-28 (22.1%) were most frequently encountered in Kangwon Province, while M-5 (20.6%), M-12 (16.2%), M-3 (13.2%), M-1 (11.8%) and M-62 (11.8%) were frequently isolated in Chungnam Province. Eighty-seven percent of strains in Kangwon produced OF while 33.2% of those in Chungnam produced OF (p< 0.0001). The difference in the distribution of serotypes and of OF production in the isolates from the children in the two provinces may be responsible for differences in the epidemiology of group A streptococcal infections and their sequelae.
Child
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Comparative Study
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Human
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Korea/epidemiology
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Residence Characteristics
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Rural Population
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Serotyping
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Streptococcal Infections/epidemiology/*microbiology
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Streptococcus pyogenes/*classification/pathogenicity
;
Virulence
10.Oral microflora of 42 patients with oral squamous cell carcinoma.
Xiao LU ; Ning GAO ; Changmei WANG ; Xiaorong XIAO
West China Journal of Stomatology 2002;20(5):356-360
OBJECTIVEThe purpose of this study was to evaluate the effects of squamous cell carcinoma on oral bacteria.
METHODSThis study investigated the microbial contents of the plaque on the surfaces of oral squamous cell carcinomas. Samples were obtained from the central surface of lesions, contiguous healthy mucosa and saliva of 42 patients with oral squamous carcinoma before and after operation.
RESULTSThe median of bacterial colony forming units per milliliter (CFUs/ml) of saliva before operation was 8.10 x 10(8) CFUs/ml. The median of CFUs per square centimeter of the tumor surface was 5.21 x 10(5) CFUs/cm2 which was significantly higher than that of the healthy (the control) mucosa (1.77 x 10(4) CFUs/cm2, P = 0.0001). The CFUs per square centimeter of mucosa surface at the operative zone was 4.34 x 10(5) CFUs/cm2 which was also significantly higher than that of the healthy (control) mucosa(7.24 x 10(4) CFUs/cm2, P = 0.0001).
CONCLUSIONOral carcinoma can break the balance of oral microflora, which may be one of the reasons leading to the high susceptivity of these compromised patients to systemic infection.
Adult ; Aged ; Bacteria, Aerobic ; isolation & purification ; Bacteria, Anaerobic ; isolation & purification ; Bacterial Infections ; etiology ; prevention & control ; Carcinoma, Squamous Cell ; microbiology ; Female ; Humans ; Male ; Middle Aged ; Mouth Mucosa ; microbiology ; Mouth Neoplasms ; microbiology ; Saliva ; microbiology ; Streptococcal Infections ; prevention & control ; Streptococcus ; isolation & purification