1.Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration.
Patrizia Casagrande PROIETTI ; Annalisa BIETTA ; Chiara BRACHELENTE ; Elvio LEPRI ; Irit DAVIDSON ; Maria Pia FRANCIOSINI
Journal of Veterinary Science 2010;11(3):221-225
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and saliva swabs were also collected from 25 animals in order to detect in vivo the presence of Helicobacter spp.. Gastric ulcers were detected in 373 cases (93%). The presence of ulcers in association with inflammatory processes was further confirmed by histological examination. Forty-nine percent (32/65) of the ulcerated esophageal portions as well as 53% (8/15) of the non-ulcerated pyloric portions were positive for Helicobacter spp. by PCR. The Helicobacter spp. positive samples were also positive for H. suis, while H. pylori was not detected. These results were confirmed by restriction enzyme analysis. With regard to feces and saliva samples, 15/25 (60%) and 16/25 (64%) were positive for Helicobacter spp. PCR, respectively but all were negative in H. suis and H. pylori specific PCR.
Animals
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Feces/*microbiology
;
Helicobacter/*isolation & purification
;
Polymerase Chain Reaction/veterinary
;
Restriction Mapping/veterinary
;
Saliva/*microbiology
;
Stomach/*microbiology
;
Stomach Ulcer/microbiology/pathology/*veterinary
;
Swine
;
Swine Diseases/*microbiology/pathology
2.Antigenic diversity and serotypes of Helicobacter pylori associated with peptic ulcer diseases.
Seon Mee PARK ; Seok Il HONG ; Hwoon Yong JUNG ; Suk Kyun YANG ; Hae Ryun KIM ; Young Il MIN ; Weon Seon HONG
The Korean Journal of Internal Medicine 1998;13(2):104-109
OBJECTIVES: Clinical presentation of Helicobacter pylori (H. pylori) infection has marked variation mainly due to the strain diversity and host susceptibility. Although H. pylori is identified as a major risk factor for gastric and duodenal ulcers, the ulcerogenic or pathogenic strain has not been documented yet. The objective of this study was to investigate antigenic types of the ulcerogenic strain of H. pylori. METHODS: The sera of 64 patients were tested by Western blot using Helicoblot 2.0 for six major anti-H. pylori antibodies, together with CLO test and histological examination of gastric biopsy tissues. Thirty-five, nine and 20 patients had duodenal ulcer, gastric ulcer and chronic active gastritis, respectively. The antigenic types of H. pylori were analyzed in 54 patients with positive H. pylori infection. In this study, H. pylori was divided into four serotypes according to the presence and absence of CagA and VagA: type I; CagA (+) and VacA(+), type Ia: CagA (+) and VacA(-), type Ib: CagA(-) and VacA(+), and type II: CagA(-) and VacA(-). RESULTS: There was no difference in the number of bands for six antigens: 3.2 +/- 1.4, 3.0 +/- 1.2 and 3.1 +/- 1.4 in 35 duodenal ulcer, 7 gastric ulcer and 12 chronic gastritis, respectively. The band with 119 kDa was 90.7%, which was the most common band with the order of 35, 30, 26.5, 89 and 19.5 kDa. Type I, la and Ib were positive in 22.2, 42.6 and 27.8%, respectively, which were significantly higher than type II (p < 0.05). There was no difference in the positive rates of four urease subtypes between the four serotypes.
Adult
;
Aged
;
Antigens, Bacterial/classification*
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Antigens, Bacterial/analysis
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Blotting, Western
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Chronic Disease
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Comparative Study
;
Duodenal Ulcer/pathology
;
Duodenal Ulcer/microbiology*
;
Duodenal Ulcer/immunology
;
Gastric Mucosa/pathology
;
Gastric Mucosa/microbiology
;
Gastritis/pathology
;
Gastritis/microbiology
;
Gastritis/immunology
;
Helicobacter Infections/immunology*
;
Helicobacter pylori/immunology*
;
Human
;
Middle Age
;
Serotyping
;
Stomach Ulcer/pathology
;
Stomach Ulcer/microbiology*
;
Stomach Ulcer/immunology
;
Substances: Antigens, Bacterial
3.Helicobacter pylori infection and gastric mucosa change and blood-lipid in people undergoing the physical examination in Changsha.
Zhiheng CHEN ; Canxia XU ; Ling LUO ; Jing XIAO ; Pingting YANG ; Chang LIU
Journal of Central South University(Medical Sciences) 2014;39(3):265-269
OBJECTIVE:
To explore the relationship between the Helicobacter pylori (H.pylori) infection and gastric mucosa change and blood-lipid in people undergoing the physical examination in Changsha.
METHODS:
A total of 2 264 people undergoing physical examination were divided into an H. pyloripositive group (n=1 068) and an H. pylori-negative group (n=1 196). Gastric mucosa change was diagnosed by gastroscopy, blood-lipid and blood sugar were detected, and the statistical analysis was performed.
RESULTS:
The incidence rate of H.pylori infection was 47.2%. The incidence rate of gastric mucosal erosion, gastric ulcer, duodenal ulcer, gastric mucosal atrophy, gastric polyp, dyslipidemia, increase of triglyceride were (TG) and decrease of the high density lipoprotein cholesterol (HDL-C) in the H.pylori-positive group were all higher than those in the H.pylori-negative group (P<0.01 or P<0.05). In the H. pylori-positive group, the level of TG in people with gastric mucosal erosion, gastric ulcer and duodenal ulcer was higher than that in people with normal gastric mucosa or mild gastritis, and HDL-C was lower than that in people with normal gastric mucosa or mild gastritis.
CONCLUSION
H. pylori infection can induce the gastric mucosa injury and dyslipidemia, which may result in the occurrence and development of coronary heart disease by increasing TG and decreasing HDL-C, thus increasing the risk of atherosclerosis.
Adenomatous Polyps
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Cholesterol, HDL
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blood
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Duodenal Ulcer
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microbiology
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physiopathology
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Dyslipidemias
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microbiology
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Gastric Mucosa
;
microbiology
;
pathology
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Gastritis
;
microbiology
;
physiopathology
;
Helicobacter Infections
;
physiopathology
;
Helicobacter pylori
;
Humans
;
Lipids
;
blood
;
Physical Examination
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Stomach Neoplasms
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Stomach Ulcer
;
microbiology
;
physiopathology
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Triglycerides
;
blood
4.Difference in the distribution pattern of Helicobacter pylori and grade of gastritis in the antrum and in the body between duodenal ulcer and benign gastric ulcer patients.
Nayoung KIM ; Wook Ryul CHOI ; Chan Ho SONG ; Dong Hyuck SHEEN ; Sang Seok YANG ; Jee Youn LEE ; Yoon Ju HAN ; Sun Hee LIM ; Kye Heui LEE ; Shin Eun CHOI
The Korean Journal of Internal Medicine 2000;15(1):32-36
OBJECTIVES: To investigate the relationship between the Helicobacter pylori (H. pylori) colonization and the grade of gastritis in the antrum and in the body of patients with duodenal ulcer (DU) or benign gastric ulcer (BGU). METHODS: This study was performed in H. pylori-positive 220 DU patients and 180 BGU patients. H. pylori density was evaluated by modified Giemsa staining and CLO test, and gastritis grade was graded by H+ACY-E staining in the antrum and in the body. RESULTS: H. pylori grade by Giemsa staining was 1.24 in the antrum and 0.82 in the body for DU group (p +ADw- 0.01), and those of BGU group were slightly reversed, 0.83 and 0.87, respectively, but without statistical significance. Similarly H. pylori grade by CLO test was 3.1 in the antrum and 2.8 in the body for DU group (p +ADw- 0.01), and those of BGU group 2.3 and 2.6 (p +ADw- 0.05), respectively. In contrast, gastritis grade was 1.7 in the antrum and 1.2 in the body for DU group (p +ADw- 0.01), and those of BGU group 1.6 and 1.3 (p +ADw- 0.01), respectively, similar to those of DU. However, there was a correlation between H. pylori grade and gastritis grade in the antrum and in the body, not only in DU but also in BGU group (p +ADw- 0.01). CONCLUSION: In spite of different distribution patterns of H. pylori between DU group and BGU group, gastritis grade of the antrum was significantly higher than that of the body in both DU and BGU. However, gastritis is correlated with H. pylori density not only in DU but also in BGU patients. It looks like the inflammatory reaction to H. pylori is stronger in the antrum than in the body.
Adult
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Aged
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Colony Count, Microbial
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Comparative Study
;
Duodenal Ulcer/pathology+ACo-
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Duodenal Ulcer/microbiology
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Female
;
Gastric Fundus/pathology
;
Gastric Fundus/microbiology
;
Gastritis/pathology+ACo-
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Gastritis/microbiology+ACo-
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Helicobacter Infections/pathology
;
Helicobacter Infections/diagnosis+ACo-
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Helicobacter pylori/isolation +ACY- purification+ACo-
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Human
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Male
;
Middle Age
;
Probability
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Pyloric Antrum/pathology
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Pyloric Antrum/microbiology
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Severity of Illness Index
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Stomach Ulcer/pathology+ACo-
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Stomach Ulcer/microbiology
5.Association of Helicobacter pylori with gastritis and peptic ulcer diseases.
Jin Kyung KANG ; Eung KIM ; Kyung Hee KIM ; Seung Heon OH
Yonsei Medical Journal 1991;32(2):157-168
The occurrence of Helicobacter pylori(H.pylori) and its relationship with gastric mucosa were studied by light and electron microscopy and culture of biopsy specimens from gastric mucosa of 160 patients with upper gastrointestinal symptoms. H. pylori were present in 96.6% of patients with active chronic gastritis, 100% of patients with duodenal ulcer and 76.9% of patients with gastric ulcer, while present in only 6.3% of individuals with histologically normal gastric mucosa. The bacteria colonized the antral mucosa more frequently than the body or than the duodenal cap mucosa. The bacteria were rarely seen in the intestinalized epithelium per se, but there was no significant difference in prevalence of H. pylori between gastritis with intestinal metaplasia and gastritis without intestinal metaplasia. H. pylori could be seen in close association with the surface of gastric epithelial cells below the mucus layer without evidence of intracellular parasitism, All of the strains tested were susceptible to penicillin, erythromycin, and most of them susceptible to tinidazole and bismuth salts. It is concluded that H. pylori are highly associated with gastritis and peptic ulcer diseases and its prevalence rates in patients with those diseases is higher than in developed countries. This strong association of H. pylori infection with gastritis and peptic ulcer diseases suggest a possible etiologic role for the bacterium in those diseases.
Adolescent
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Adult
;
Aged
;
Chronic Disease
;
Duodenal Ulcer/*microbiology/pathology
;
Female
;
Gastric Mucosa/microbiology/pathology/ultrastructure
;
Gastritis/*microbiology/pathology
;
*Helicobacter Infections
;
Helicobacter pylori/*isolation & purification
;
Human
;
Male
;
Middle Aged
;
Prospective Studies
;
Stomach Ulcer/*microbiology/pathology
;
Support, Non-U.S. Gov't
6.Gastric syphilis: report of a case.
Yun DONG ; Ming-xia WU ; Jing TANG ; Yi-zhong FENG
Chinese Journal of Pathology 2011;40(3):198-199
Adolescent
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Anti-Bacterial Agents
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therapeutic use
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Anti-Ulcer Agents
;
therapeutic use
;
Female
;
Humans
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Penicillin G Procaine
;
therapeutic use
;
Stomach Ulcer
;
drug therapy
;
microbiology
;
pathology
;
Syphilis
;
drug therapy
;
microbiology
;
pathology
;
Syphilis Serodiagnosis
;
methods
;
Treponema pallidum
;
isolation & purification
7.MG7 expression in helicobacter pylori-related gastric diseases.
Dongli GUO ; Ming DONG ; Yuan YUAN
Chinese Journal of Oncology 2002;24(2):151-153
OBJECTIVETo determine the influence of Helicobacter pylori (H. pylori) on gastric cancer-related antigen MG7 expression.
METHODSThe H. pylori infection and the expression level of antigen MG7 in gastric mucosa were determined by HE stain, PCR, ELISA and immunohistochemistry in 291 patients with H. pylori-related conditions, among whom 34 were followed-up.
RESULTSNo significant difference was found between H. pylori-negative and H. pylori-positive intestinal metaplasia, atrophic gastritis and dysplasia of gastric epithelium in positive rate of antigen MG7 expression. There was significant difference between H. pylori-negative and H. pylori-positive superficial gastritis in the positive rate of MG7 expression (P < 0.05). During follow-up, one of 3 H. pylori-negative cases turned to be H. pylori-positive, and its MG7 expression turned to be higher at the same time. Three of 31 H. pylori-positive patients were discovered as having early gastric cancer, among whom one with antigen MG7 expression (+ + +) was found to have a reduced Mg7 expression accompanied with H. pylori eliminutied after operation.
CONCLUSIONThere is correlationship between H. pylori infection and MG7 expression in superficial gastritis. Although the MG7-positive lesions with H. pylori infection shows a benign nature in morphology, they also have the potential risk of developing into gastric cancer. Therefore, they should be followed up, during which special attention should be paid to patients with increased MG7 expression.
Antibodies, Bacterial ; blood ; Antigens, Neoplasm ; biosynthesis ; DNA, Bacterial ; genetics ; Enzyme-Linked Immunosorbent Assay ; Gastric Mucosa ; metabolism ; microbiology ; pathology ; Gastritis ; metabolism ; microbiology ; Helicobacter Infections ; metabolism ; microbiology ; Helicobacter pylori ; genetics ; growth & development ; immunology ; Humans ; Immunohistochemistry ; Polymerase Chain Reaction ; Stomach Diseases ; metabolism ; microbiology ; Stomach Ulcer ; metabolism ; microbiology
8.A Case of Cronkhite-Canada Syndrome Showing Resolution with Helicobactor pylori Eradication and Omeprazole.
Myung Shin KIM ; Hye Kyung JUNG ; Hae Sun JUNG ; Ju Young CHOI ; Yoon Ju NA ; Gun Woo PYUN ; Jung Hwa RYU ; Il Hwan MOON ; Min Sun JO
The Korean Journal of Gastroenterology 2006;47(1):59-64
We describe a 58-year-old woman who was incidentally found to have gastric and colonic polyposis, hypoalbuminemia, cutaneous hyperpigmentation and onychodystrophy (Cronkhite-Canada syndrome). Histology of polyps from the stomach showed features of juvenile or retention type (hamartomatous) polyps with Helicobacter pylori (H. pylori) infection. The large pedunculated colonic polyps showed hamartomatous polyps with adenomatous component and polypectomy was performed. After the treatment with H. pylori eradication and omeprazole, the gastric polyposis, hypoalbuminemia and anemia regressed, and endoscopic polypectomy of gastric polyps were performed. After the continuous use of omeprazole for 14 months, the patient showed complete resolution of clinical features of Cronkhite-Canada syndome. The experience of this case suggests that eradication of H. pylori and proton pump inhibitor treatment might be considered in patients with gastric polyposis combined with Cronkhite-Canada syndome.
Anti-Ulcer Agents/*therapeutic use
;
Colonic Polyps/complications/microbiology/pathology
;
Female
;
Helicobacter Infections/complications/*drug therapy
;
*Helicobacter pylori
;
Humans
;
Hyperpigmentation/pathology
;
Middle Aged
;
Nails, Malformed/pathology
;
Omeprazole/*therapeutic use
;
Polyps/*complications/microbiology/pathology
;
Proton Pumps/antagonists & inhibitors
;
Stomach Neoplasms/*complications/microbiology/pathology
;
Syndrome