1. HSV-1 H129-Derived Anterograde Neural Circuit Tracers: Improvements, Production, and Applications
Hong YANG ; Feng XIONG ; Yi-Ge SONG ; Hai-Fei JIANG ; Hai-Bin QIN ; Jing ZHOU ; Wen-Bo ZENG ; Min-Hua LUO ; Hong YANG ; Feng XIONG ; Yi-Ge SONG ; Hai-Fei JIANG ; Hai-Bin QIN ; Jing ZHOU ; Min-Hua LUO ; Sha LU ; Steven F. GRIECO ; Xiangmin XU ; Fei ZHAO ; Fei ZHAO ; Min-Hua LUO
Neuroscience Bulletin 2021;37(5):701-719
Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1 (HSV-1) strain H129 (H129), and have been successfully applied to map diverse neural circuits. Initially, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.
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