1.Study on steroids of Sedum lineare.
Xiaofeng NIU ; Xia LIU ; Lan PAN ; Lin QI
China Journal of Chinese Materia Medica 2011;36(10):1319-1321
OBJECTIVETo isolate and identify the steroids in Sedum lineare.
METHODThe steroids were isolated by column chromatography, semi-preparative thin layer chromatography and related techniques, their structures were elucidated through spectroscopic analyses.
RESULTSix steroids were isolated and identified as stigmast-7-en-3beta-ol (1), stigmast-3beta,5alpha,6beta-triol (2), stigmast-5-en-3beta-ol-7-one (3), stigmast-5-en-3beta,7alpha-diol (4), stigmast-5-en-3beta,7beta-diol (5), beta-sitosterol (6).
CONCLUSIONCompounds 1-5 were isolated from this plant for the first time.
Plant Extracts ; analysis ; Sedum ; chemistry ; Steroids ; analysis ; isolation & purification
2.Most advance on chemical and biological investigations of gorgonian-octocorals.
Xingyun CHAI ; Liying TANG ; Hui LEI ; Changcai BAI ; Jianfan SUN ; Yunqiu LI ; Yonghong LIU
China Journal of Chinese Materia Medica 2012;37(5):667-685
This review presents the most recent chemical and biological investigations on one of the marine invertebrates, gorgonian octocoral. It summarizes all 432 new compounds published in 2002-2009, which consists of 46 sesquiterpenoids (including 2 new natural products, NNP), 282 diterpenoids (including 4 from Pennatulacea octocoral and one artifact), 76 steroids, and 29 alkaloids or other types (2 NNP included). In this paper, according to the structure division, the new compounds are described in combination with the taxonomy of the investigated animals, and its simultaneous bioactivity results. Novel skeletons and complex structures are paid most emphasis on its features, means of structural elucidation and the proposed biogenesis pathway. The source of all new compounds and the different diterpenoid skeleton types are all listed and analyzed, as well as the commonly used Chinese names or some proposed ones for diterpenoid skeletons. Furthermore, this papers deals with all biological test toward the gorgonian new metabolites, i.e. anti-cancer, anti-inflammatory, anti-bacterial(against Staphylococcus aureus bacteria, Mycobacterium tuberculosis etc), anti-malaria, and anti-fouling as well, in which anti-cancer activity and cytotoxicity were additionally, a discussion and prospect are proposed regarding chemical overview on gorgonian. This review, hopefully, can be useful in proving data and references for further chemical and biological research of China sea gorgonian, for the studies on chemical ecology, and for the discovery of new and bioactive compounds or the marine-derived leading targets.
Alkaloids
;
analysis
;
Animals
;
Anthozoa
;
chemistry
;
physiology
;
Diterpenes
;
analysis
;
Sesquiterpenes
;
analysis
;
Steroids
;
analysis
3.Flavone and steroid chemical constituents from rhizome of Paris axialis.
Xianxiao HUANG ; Wenyuan GAO ; Wanshun ZHAO ; Tiejun ZHANG ; Jun XU
China Journal of Chinese Materia Medica 2010;35(22):2994-2998
OBJECTIVETo study the chemical constituents from the rhizome of Paris axialis.
METHODThe compounds were isolated by column chromatography with silica gel and purified by Sephadex LH-20 column chromatography and preparative RP-HPLC. The structures were identified by means of spectroscopic methods.
RESULTFourteen compounds were isolated from the EtOAc extract and the n-BuOH extract of P. axialis. Their structures were identified as daucosterol (1), stigmasterol-3-O-beta-D-glycopyranoside (2), beta-ecdysterone (3), pennogenin-3-O-alpha-L-arabinofuranosyl (1 --> 4) -[alpha-L -rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (4), diosgenin-3-O-alpha-L- rhamnopy-ranosyl (1 --> 4) -alpha-L-rhamnopyranosyl (1 --> 4) [alpha-L-rhamnopyranosyl (1 --> 2)] -beta-D-glycopyranoside (5), kaempferol (6), rutin (7), myrincitrin (8), 4, 2', 4'-trihydroxychalcone (9), isorhamnetin-3-O-beta-D- glycopyranoside (10), isorhamnetin-3-O-alpha-L-rhamnopyranosyl (1 --> 2) -beta-D-glycopyranoside (11), isorhamnetin-3-O-beta-D-glucpyranosyl (1 --> 6) -beta-D-glycopyranoside (12), kayaflavone (13), amentoflavone (14).
CONCLUSIONCompounds 1-3 and 6-14 are isolated from P. axialis for the first time; and compounds 7-10, 13, 14 are isolated from the genus Paris for the first time.
Flavones ; analysis ; isolation & purification ; Liliaceae ; chemistry ; Plant Extracts ; analysis ; isolation & purification ; Rhizome ; chemistry ; Steroids ; analysis ; isolation & purification
4.The Early Onset of Disease May Be a Risk Factor for Decreased Bone Mineral Density in Patients with Inflammatory Bowel Disease.
Hwa Jong KIM ; Su Jin HONG ; Young Woo JEON ; Jae Pil HAN ; Seung Hyo HAN ; Jee Heon KANG ; Jae Woong TAE ; Hee Sook LIM ; Hee Kyung KIM ; Bong Min KO ; Moon Sung LEE
Clinical Endoscopy 2013;46(1):71-76
BACKGROUND/AIMS: The pathogenesis of bone loss in patients with inflammatory bowel disease (IBD) is complex, multifactorial, and only partly understood. We aimed to examine the extent and risk factors of bone mass reduction and to analyze the impact of early onset of a disease before attaining peak bone mass in IBD patients. METHODS: We compared the risk factors for osteoporosis and BMD at the lumbar spine and the hip bone in IBD patients. RESULTS: A total of 44 patients with IBD were enrolled. Twenty-one and 23 patients were diagnosed as IBD before and after the age of 30 and designated as group A and group B, respectively. Group A had significant bone mass reduction at the lumbar spine than group B (BMD, 1.01+/-0.10 vs. 1.14+/-0.17, p<0.01; T-score, -1.22+/-0.84 vs. -0.08+/-1.39, p<0.01; Z-score, -1.11+/-0.81 vs. -0.03+/-1.32, p<0.01, respectively). Multivariate analysis showed that patients diagnosed as IBD before the age of 30 had possible risk factor of bone mass reduction (hazard ratio, 3.96; p=0.06). CONCLUSIONS: Bone mass reduction was more severe in patients who were diagnosed with IBD before the age of 30 than in those diagnosed after the age of 30.
Bone Density
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Hip
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Humans
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Inflammatory Bowel Diseases
;
Multivariate Analysis
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Osteoporosis
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Risk Factors
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Spine
;
Steroids
5.Steroid and triterpenoid saponins from the rhizomes of Paris polyphylla var. stenophylla.
Jinming HU ; Yunyang LU ; Shuxian ZHENG ; Yunyuan TIAN ; Tianyi LI ; Haifeng TANG ; Zhao YANG ; Yang LIU
Chinese Journal of Natural Medicines (English Ed.) 2023;21(10):789-800
Five new saponins, including three steroid saponins, paristenoids A-C (1-3), and two triterpenoid saponins, paristenoids D-E (4-5), along with four known ones (6-9) were isolated from the rhizomes of Paris polyphylla var. stenophylla. The structures of the isolated compounds were identified mainly by detailed spectroscopic analysis, including extensive 1D and 2D NMR, MS, as well as chemical methods. Compound 3 is a new cyclocholestanol-type steroidal saponin with a rare 6/6/6/5/5 fused-rings cholestanol skeleton, and this skeleton has been first found from the genus Paris. The cytotoxicities of the isolated compounds against three human three glioma cell lines (U87MG, U251MG and SHG44) were evaluated, and compound 7 displayed certain inhibitory effect with IC50 values of 15.22 ± 1.73, 18.87 ± 1.81 and 17.64 ± 1.69 μmol·L-1, respectively.
Humans
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Rhizome/chemistry*
;
Steroids/chemistry*
;
Liliaceae/chemistry*
;
Saponins/chemistry*
;
Triterpenes/analysis*
6.Determination of C21 steroidal glycosides in Marsdenia tenacissima by colorimetric method.
China Journal of Chinese Materia Medica 2005;30(22):1745-1748
OBJECTIVETo establish a quantitative method to deter mine C21 steroidal glycosides in Marsdenia tenacissima.
METHODMethanol was used as the extraction solvent and the samples were purified by macroporous resin ADS-7. A mixture of sulfuric acid-methanol (4:1)was used as color-producing reagent. The absorbance was measured at 325 nm.
RESULTThere is a good linearity (r = 0.999 9, n = 6) within the range of 10.6-148.4 microg. The average recoveries of tenacissoside-H at different concentrations were 95.8% to 97.1% with RSD less than 2.4% (n = 5).
CONCLUSIONThis method is reliable as a quantitative analytical method for the quality assessment of M. tenacissima.
Colorimetry ; methods ; Glycosides ; analysis ; Marsdenia ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Powders ; Quality Control ; Reproducibility of Results ; Steroids ; analysis
7.The applications and challenges of liquid chromatography tandem mass spectrometry in maternal and child health.
Lin LIU ; Zheng Wen XU ; Lan Lan MENG ; Yi Fan LU ; Yan Hong ZHAI ; Zheng CAO
Chinese Journal of Preventive Medicine 2022;56(10):1520-1526
Due to its ultra-high sensitivity, specificity and throughput, liquid chromatography tandem mass spectrometry (LC-MS/MS) has become an important analytical tool in clinical laboratories in quantifying various small molecules, such as vitamins, bile acids, steroids and other internal metabolites relevant to maternal diseases. As an effective means of screening and diagnosing diseases in preventive medicine, LC-MS/MS has been widely used in maternal and child health, contributing to the reduction of the incidence of maternal and child diseases and premature morbidity and mortality. At present, LC-MS/MS is an emerging and powerful platform in laboratory testing in China, facing both challenges and opportunities. In this article, the representative applications in the field of maternal and child health are summarized and discussed, along with the major hurdles of LC-MS/MS in clinical recognition and implementation.
Child
;
Humans
;
Chromatography, Liquid/methods*
;
Tandem Mass Spectrometry/methods*
;
Child Health
;
Steroids/analysis*
;
Vitamins/analysis*
;
Bile Acids and Salts
8.Identification of chemical constituents in qiliqiangxin capsule by UPLC-Q-TOF/MS(E).
Li-ping KANG ; Yang ZHAO ; He-shui YU ; Yi-xun LIU ; Cheng-qi XIONG ; Da-wei TAN ; Ji-ming JIA ; Hong-tao WANG ; Shu-yan TIAN ; Bai-Ping MA
Acta Pharmaceutica Sinica 2011;46(10):1231-1236
In order to clarify the chemical constituents in Qiliqiangxin capsule, a rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry (UPLC-Q-TOF/MS(E)) method was established. Forty peaks were identified on line using this method. The herbal sources of these peaks were assigned. The results implied that triterpenoid saponins, flavonoid glycosides, C21-steroids and phenolic acids were included in the main components of Qiliqiangxin capsule. The method is simple and rapid for elucidation of the constituents of Qiliqiangxin capsule and the results are useful for the quality control of Qiliqiangxin capsule.
Capsules
;
Chromatography, High Pressure Liquid
;
Drugs, Chinese Herbal
;
chemistry
;
Flavones
;
analysis
;
Ginsenosides
;
analysis
;
Glycosides
;
analysis
;
Hydroxybenzoates
;
analysis
;
Plants, Medicinal
;
chemistry
;
Quality Control
;
Saponins
;
analysis
;
Spectrometry, Mass, Electrospray Ionization
;
Steroids
;
analysis
;
Tandem Mass Spectrometry
;
Triterpenes
;
analysis
9.Two new steroidal alkaloids from ripe berries of Solanum nigrum.
Yu-Ke YANG ; Liang-Yu LIU ; Wen-Yi WANG ; Jian-Nong WANG ; Li LIN ; Jian-Xun LIU
China Journal of Chinese Materia Medica 2022;47(18):4966-4971
Two previously undescribed steroidal alkaloids, compounds 1-2, along with two known ones(3-4), were isolated from the 80% ethanol extract of ripe berries of Solanum nigrum by chromatographic methods, including silica gel, ODS, and HPLC. Based on spectroscopic and chemical evidence, including IR, NMR, and HR-ESI-MS data, the structures of the isolated compounds were identified as 12β,27-dihydroxy solasodine-3-O-β-D-glucopyranoside(1), 27-hydroxy solasodine-3-O-β-D-glucopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→4)]-β-D-glucopyranoside(2), solalyraine A(3), and 12β,27-dihydroxy solasodine(4). Compounds 1-2 were tested for their potential effects against the proliferation of A549 cells, which revealed that compounds 1-2 had weak cytotoxic activity.
Alkaloids/analysis*
;
Ethanol
;
Fruit/chemistry*
;
Molecular Structure
;
Plant Extracts/chemistry*
;
Saponins/analysis*
;
Silica Gel/analysis*
;
Solanum/chemistry*
;
Solanum nigrum/chemistry*
;
Steroids/pharmacology*
10.Tinea Incognito on the Face and Neck.
Jin PARK ; Yong Sun CHO ; Ju Ik KIM ; Dae Woo KIM ; Su Han WOO ; Chin Ho RHEE ; Seok Kweon YUN ; Hau Uk KIM
Korean Journal of Medical Mycology 2014;19(3):71-75
Tinea incognito is a dermatophytosis modified by previous treatments such as prolonged use of corticosteroids. Because the lesions often atypical appearing without classic features of ringworm, the diagnosis can be delayed or missed especially when tinea incognito is on the face and neck. A 75-year-old male patient who had been treated with topical steroids at the local dermatologic clinic for 10 years for the eczematous lesion on the scalp and face and recently the lesions spread throughout his neck and back. Physical examination showed ill-defined erythematous scaly patches on the face, neck and back and reddish crusted papules on the scalp. Potassium hydroxide (KOH) preparation on the left face and right neck showed hyphae and fungus cultures were positive for colonies of Trichophyton rubrum (T. ruburum). The result of DNA sequencing from the colony was identical to that of T. rubrum. The lesions were treated by oral terbinafine and topical isoconazole.
Adrenal Cortex Hormones
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Aged
;
Diagnosis
;
Fungi
;
Humans
;
Hyphae
;
Male
;
Neck*
;
Physical Examination
;
Potassium
;
Scalp
;
Sequence Analysis, DNA
;
Steroids
;
Tinea*
;
Trichophyton