Purpose: The aim of this study was to investigate the mechanical properties of three-dimensional (3D) printed denture base resin incorporating microcapsules containing plant essential oils. Materials and Methods: Denture base specimens containing up to 3% w/v essential oil microcapsule powders (MCPs), i.e., eucalyptus, geranium, lavender, menthol, and tea tree, in two resins (Detax and NextDent 3D+) were 3D printed using two printers (Asiga and NextDent 5100). The dispersion and interaction of the MCPs in the resin were assessed by SEM while the mechanical properties of the incorporated denture base including flexural strength (MPa), flexural modulus (MPa), Vickers hardness (VHN), and surface roughness (Ra) were also subsequently evaluated. Statistical analysis of any differences in mean values was determined using a two-way ANOVA with Tukey’s post hoc testing (α = .05). Results: The spherical shape of the MCPs was maintained during the mixing and polymerization/printing process. However, the Detax-Asiga group showed significant agglomeration of the MCPs even at the lowest MCP concentration levels (0.5% w/v). Overall, as the microcapsule concentration increased, the mean flexural strength decreased, though the menthol MCP groups remained compliant with the ISO standard. The flexural modulus and harness remained relatively unchanged, and the flexural modulus complied with the ISO standard regardless of the MCP concentration. Surface roughness increased with the addition of the MCPs but also remained below that required for clinical acceptance. Conclusion Incorporation of microencapsulated plant essential oils into 3D printed denture base resin was successfully achieved. While incorporation negatively influenced flexural strength and surface roughness, little effect on flexural modulus and Vickers hardness was demonstrated.