1.Staphylococcus aureus carriage in selected kindergartens in Klang Valley
Nurul Azmawati Mohamed ; Shalinawati Ramli ; Nur Natasha Zulkifli Amin ; Wan Shahida Wan Sulaiman ; Ilina Isahak ; Tengku Zetty Maztura Tengku Jamaluddin ; Nooriah Mohammed Salleh
The Medical Journal of Malaysia 2016;71(2):62-65
Introduction: Nasal colonisation of S. aureus in healthy
children was 18% to 30%. One to three percent of them were
colonised by Methicillin-resistant Staphlycoccus aureus
(MRSA). Although MRSA infection has become increasingly
reported, population-based S. aureus and MRSA
colonisation estimates are lacking. The main objective of
this study was to determine the prevalence of S. aureus
carriage among children.
Methods: Nasal samples for S. aureus culture were obtained
from 250 children from three kindergartens in the Klang
Valley, after consent was obtained from the children and
their parents. Swabs were transported in Stuart medium,
and inoculated on mannitol-salt agar within four hours of
collection. Identification and disk diffusion test were done
according to guidelines. Polymerase chain reaction was
done on MRSA isolates for the presence of mecA and lukS/FPV
genes.
Results: Overall prevalence of S. aureus and MRSA carriage
were 19.2% (48/250) and 1.6% (4/250) respectively. mecA
gene was present in all isolates, 50% isolates carried
Panton-Valentine leucocidin (PVL) gene. Sccmec type I was
found in 2 isolates and the remaining isolates has Sccmec
type V.
Conclusion: The prevalence of S. aureus and MRSA carriage
were similar to other studies. However, risk of contracting
severe infection might be higher due to presence of PVL
gene in half of the MRSA isolates.
Staphylococcus aureus
2.Mycotic bronchial artery aneurysmal rupture in the early stage of lung abscess: A case report
Mohd Alkaf Ab Latip ; Syed Rasul Syed Hamid ; Abdul Rahman Ismail
The Medical Journal of Malaysia 2016;71(2):96-97
Symptomatic bronchial artery aneurysm warrants urgent
intervention. It has a known association with pulmonary
infection caused by Staphylococcus aureus. We hereby
report an elderly lady with a ruptured left superior bronchial
artery mycotic aneurysm. She was in the early stages of
treatment for a left lung abscess. She had multiple episodes
of haemoptysis following which she underwent a left lower
lobectomy. Presentation of lung abscess with a concurrent
ruptured mycotic aneurysm warrants early surgical
intervention and can be curative as seen in this case.
Staphylococcus aureus
3.Presence of antimicrobial resistant Staphylococcus aureus in chicken meat and its potential public health implications
Erkihun Aklilu ; Hurul Ain Ab Manah ; Nurhardy Abu Daud
Malaysian Journal of Microbiology 2016;12(6):418-422
Aim: Multi-drug resistant bacteria have become a global issue. Drug-resistant bacteria can be found in humans,
animals, food and environmental sources. Staphylococcus aureus is one of many bacteria species known for its
antimicrobial resistance. The current study is conducted to determine the antimicrobial resistance profiles of S. aureus
isolated from raw chicken meat samples in Kota Bharu, Kelantan.
Methodology and results: Fifty raw and fresh chicken meat samples were purchased from 3 different wet markets in
Kota Bharu, Kelantan and were transported to the laboratory aseptically. Routine isolation and identification of S. aureus
was conducted and the isolates were confirmed by polymerase chain reaction (PCR) through the detection of a S.
aureus specific gene, nucA. Antimicrobial sensitivity tests were conducted according to Kirby-Bauer methods (Hudzicki,
2013). Staphylococcus aureus was isolated in 24% (12/50) of the samples. All the isolates were resistant towards at
least two of the antimicrobials tested. Of these, 11 (91.67%), 10 (83.33%), 5 (41.67%), 3 (25%), 1 (8.33%) and 1
(8.33%) were resistant to ampicillin (AMP10), teicoplanin (TE30), amoxicillin (AML10), penicillin (P10), oxacillin (OX1)
and mupirocin (MUP20) respectively. In addition to that, all the isolates were susceptible to streptomycin, vancomycin,
teicoplanin and cefoxitin. However, all the isolates were negative for the methicillin resistance encoding gene, mecA
while one of the isolates showed resistance towards oxacillin.
Conclusion, significance and impact of the study: The results from this study indicated that raw chicken intended for
human consumption may be contaminated by antimicrobial-resistant strains of S. aureus. This may lead to the
colonization or infection in humans. Nevertheless, further detailed investigation to determine the correlation between
contamination of chicken meat and colonization of antimicrobial resistant S. aureus should be carried out. The relevance
of the present study which showed contamination of fresh chicken meat with antimicrobial resistant S. aureus
emphasizes the need to have stricter hygiene measures for retailers during the handling of the chicken meat to minimize
or avoid possible health hazards for consumers.
Staphylococcus aureus
4.Comparative proteomics profiling reveals down-regulation of Staphylococcus aureus virulence in achieving intermediate vancomycin resistance
Xin-Ee Tan ; Hui-min Neoh ; Mee-Lee Loo ; Toh Leong Tan ; Salasawati Hussin ; Longzhu Cui ; Keiichi Hiramatsu ; Rahman Jamal
Malaysian Journal of Microbiology 2016;12(6):498-505
Aims: VraSR and GraSR were shown to be important in conferring intermediate vancomycin resistance in VISA.
Nevertheless, the exact mechanism modulated by these systems leading to the development of VISA remains unclear.
We employed a proteomic approach to determine the VraS and GraR regulons and subsequently derive the possible
vancomycin resistance regulatory pathway(s) in the Mu50 lineage of Staphylococcus aureus.
Methodology and results: Staphylococcus aureus strains Mu50Ω, Mu50Ω-vraSm and Mu50Ω-vraSm-graRm are
isogenic strains with ascending levels of vancomycin resistance. Total proteins were extracted from the 3 strains and
trypsin digested prior to protein isolation and identification by LC-ESI MS/MS and PLGS 2.4. Expression profiles of
resulting proteins were analyzed using Progenesis LC/MS software. Differential expression profiles revealed 3 regulons,
each controlled by VraS (Mu50Ω-vraSm vs Mu50Ω), GraR (Mu50Ω-vraSm-graRm vs Mu50Ω-vraSm) and VraS-GraR
(Mu50Ω-vraSm-graRm vs Mu50Ω), respectively. The regulon down-regulated by VraS in Mu50Ω-vraSm were proteins
associated with virulence (MgrA, Rot, and SarA), while GraR up-regulated resistance-associated proteins (TpiA, ArcB
and IsaA) in Mu50Ω-vraSm-graRm. The VraS-GraR regulon mediated both up-regulation of resistance-associated
proteins (ArgF, ArcB, VraR and SerS) and down-regulation of virulence-associated protein GapB.
Conclusion, significance and impact of study: Down-regulation of virulence- in concert with up-regulation of
resistance-associated proteins appears to be integral for development of intermediate-vancomycin resistance in the
Mu50 lineage of S. aureus.
Staphylococcus aureus
5.Detection of virulence genes and antibiotic resistance profiles of Staphylococcus aureus isolated from animals
Asinamai Athliamai Bitrus ; Zakaria Zunita ; Siti Khairani Bejo ; Sarah Othman ; Nur Adilah Ahmad Nadzir
Malaysian Journal of Microbiology 2016;12(6):408-417-417
Aims: This study was designed to determine the virulence genes and antibiotic resistance profiles of Staphylococcus
aureus isolated from dogs, cats, chickens and horses.
Methodology and results: A total of 15 S. aureus isolates were used in this study. Antibiogram and screening of
virulence genes was carried out using disc diffusion method and polymerase chain reaction. The results obtained
showed that a total of 9 S. aureus isolates were resistant towards oxacillin (60%), 9 isolates were resistant towards
neomycin (60%) and 8 isolates were resistant towards tilmicosin (53%). Resistance to amoxicillin, tetracycline and
vancomycin was also observed in 6 (40%) of the isolates. Additionally, 5 (33%) of the isolates showed resistance
towards streptomycin and linzolide while 4 (27%) of the isolates were resistant towards rifampin, erythromycin and
mupirocin. Lastly, 3 (20%) of the isolates were resistant towards doxycycline. Intermediate resistance to amoxicillin and
doxycycline was also observed. Virulence gene profiling showed that 4 (26.7%) of the isolates were positive for hlβ and
SspA, 9 of the isolates (60%) showed positive for geh and 12 of the isolates (80%) showed positive for Set-1. Similarly,
2 (13.3%) of the isolates showed positive for etA and Seu while only 1 isolate (6.7%) showed positive for PVL and hlα.
None of the isolates were positive for tst-1 and etB.
Conclusion, significance and impact of study: This study revealed reduced susceptibility and multiple drug
resistance (MDR) in four isolates, and susceptibility to all antibiotics in two isolates in addition to low carriage rate of
virulence gene in all isolates. Thus, indicating resistance development in majority of the isolates and the need to regulate
indiscriminate use of antibiotics in animals.
Staphylococcus aureus
6.Binding of fibronectin to staphylococcus aureus.
Jung Wan KIM ; Sang Hwa LEE ; Yoo Chul LEE ; Sung Yong SEOL ; Dong Taek CHO
Journal of the Korean Society for Microbiology 1993;28(6):431-441
No abstract available.
Fibronectins*
;
Staphylococcus aureus*
;
Staphylococcus*
7.In vitro antibacterial activity of WCM 302, a substance obtained from culture filtrate of streptomyces sp. 302, against staphylococcus aureus.
Woon Seob SHIN ; Joo Young PARK ; Choon Myung KOH
Journal of the Korean Society for Microbiology 1992;27(6):493-500
No abstract available.
Staphylococcus aureus*
;
Staphylococcus*
;
Streptomyces*
8.Vancomycin-resistant Staphylococcus aureus.
Korean Journal of Infectious Diseases 2001;33(1):62-70
No abstract available.
Staphylococcus aureus*
;
Staphylococcus*
9.Vancomycin-resistant Staphylococcus aureus.
Korean Journal of Infectious Diseases 2001;33(1):62-70
No abstract available.
Staphylococcus aureus*
;
Staphylococcus*
10.Molecular and genetic charaterization of antimicrobial resistance in staphylococcus aureus.
Journal of the Korean Society for Microbiology 1991;26(1):1-23
No abstract available.
Staphylococcus aureus*
;
Staphylococcus*