1.The Effectiveness of Red Pomegranate (Punica granatum Linn) Extract Mouthwash Against the Number of Oral Bacteria Colony
Sri Hernawati ; Balqis Salsabila Setya Aldianah ; Pujiana Endah ; Anis Irmawati
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 4, July):26-29
Introduction: The percentage of Indonesian population experiencing oral and dental diseases based on the 2007
and 2013 RISKESDAS data, rise from 23.2% to 25.9%. People need to maintain healthy teeth and mouth by brushing
their teeth and using mouthwash. Chlorhexidine is mouthwash that has been proven to be effective in reducing the
number of plaque microorganisms attached to teeth and oral mucosa, but its long-term use can cause tooth stain,
allergies, tissue desquamation and resistance. Red pomegranate can be used as an alternative because it contains
flavonoids and tannins which are thought to have antibacterial ability. Therefore, this study aims to prove that red
pomegranate extract mouthwash can reduce the number of oral bacteria colonies. Methods: 25 subjects were divided into 5 groups, namely K1: gargle aquades; K2: Chlorhexidine mouth rinse; K3, K4, K5 each rinse with 0.02%,
0.2% and 2% red pomegranate extract mouthwash. Saliva is collected before and after gargling, then planted in Petri
dish using BHIA media with a spread plate method. Calculation of the amount of bacterial colony done 24 hours after
the media was incubated. Counting the number of bacterial colonies using a colony counter. Data were analyzed
by Anova and LSD tests. Result: There were significant difference between all group on decreased of number of oral
bacteria colony (p= 0.00). Conclusion: Red pomegranate extract mouthwash effective decreased of number of oral
bacteria colony and the most effective was 2% concentration.