3.Progress in the relationship between head and neck squamous cell carcinom and the microbial community.
Chenyang LIU ; Yujun LI ; Zhen DONG ; Sen ZHANG ; Hui HUANGFU ; Yue HAN ; Miao CHANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2023;37(6):498-502
Microorganisms are one of the important factors which maintain the homeostasis of human health. Despite recent advances, the relationship between microorganisms and head and neck squamous cell carcinoma (HNSCC) is still unclear, and the impact of microorganisms on the incidence and prognosis of HNSCC cannot be neglected. Therefore, this article provides a systematic and comprehensive review summarizing the epidemiological evidence of microbial dysbiosis related to HNSCC and discusses the associations between them.
Humans
;
Carcinoma, Squamous Cell/pathology*
;
Epithelial Cells
;
Head and Neck Neoplasms
;
Microbiota
;
Prognosis
;
Squamous Cell Carcinoma of Head and Neck
4.Expression and clinical significance of MICA in laryngeal carcinoma tissue and cells.
Rui WANG ; Junfu WANG ; Qinghong SU ; Xuemei CHEN ; Xiaoqun XU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2016;30(2):94-97
OBJECTIVE:
To investigate the mRNA and protein expression of MICA in laryngeal squamous cell carcinoma tissue and the Hep-2 cells.
METHOD:
Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western-blot were used to detect the expression of MICA mRNA and protein levels in the Hep-2 cells and laryngeal cancer tissues.
RESULT:
The MICA mRNA showed higher expression in Hep-2 cells by RT-PCR. Compared with the control, the mRNA expression of MICA was significantly enhanced in laryngeal cancer tissues (t = 11.878, P < 0.01). The intensity of MICA expression is not related to the clinical stage of cancer. MICA protein demonstrated higher level expression by Western blot. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues.
CONCLUSION
The MICA mRNA showed stronger expression in Hep-2 cells and laryngeal cancer tissues. The intensity of its expression is not related to clinical stage of cancer. The MICA protein expression was strong in Hep-2 cells. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues. MICA may play an important role in laryngeal carcinoma process.
Carcinoma, Squamous Cell
;
metabolism
;
pathology
;
Cell Line, Tumor
;
Head and Neck Neoplasms
;
metabolism
;
pathology
;
Histocompatibility Antigens Class I
;
metabolism
;
Humans
;
Laryngeal Neoplasms
;
metabolism
;
pathology
;
Squamous Cell Carcinoma of Head and Neck
5.Relationship between vasculogenic mimicry and clinic pathological features in laryngeal carcinoma.
Yan FENG ; Binquan WANG ; Gang LIANG ; Shuxin WEN ; Ruifang SUN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(23):2071-2075
OBJECTIVE:
To investigate the presence of vasculogenic mimicry in laryngeal squamous cell carcino- ma and explore its clinical significance.
METHOD:
The presence of vasculogenic mimicry and expression of endotheli- um-dependent vessel in 138 laryngeal squamous cell carcinomas cases were detected by the immunohistochemistry and tissue microarray. Metlab software was used to evaluate the relationship among vasculogenic mimicry, mi- crovessel density and clinic pathological parameters in laryngeal carcinoma.
RESULT:
We found vasculogenic mimicry in 32 (26.23%) of 122 laryngeal carcinoma samples. The mean of microvessel density is 12.61 per high-power field. The vasculogenic mimicry and expression of endothelium-dependent vessel were not significantly related to patient age or gender, tumor location, pathology grade, T stage or N stage (P > 0.05). However, the vasculo- genic mimicry and the mean of microvessel density were a little higher in patients older than 60, with poorly differ- entiated and patients with N₁₋₃ stage. Vasculogenic mimicry was positively correlatedwith microvessel density (r = 0.1927, P < 0.05).
CONCLUSION
Vasculogenic mimicry can occur in laryngeal carcinoma. Moreover, vasculogenic mimicry may be associated with recurrence and metastasis in laryngeal carcinoma.
Carcinoma, Squamous Cell
;
pathology
;
Endothelium, Vascular
;
pathology
;
Head and Neck Neoplasms
;
pathology
;
Humans
;
Immunohistochemistry
;
Laryngeal Neoplasms
;
pathology
;
Neoplasm Recurrence, Local
;
Neovascularization, Pathologic
;
Squamous Cell Carcinoma of Head and Neck
7.A case of branchiogenic carcinoma.
Lin SHI ; Tiebing SHEN ; Qinnuan SUN ; Xinlai ZHANG ; Hongwei YUAN
Chinese Journal of Stomatology 2015;50(12):768-769
9.Invasion and metastasis mechanism of human papillomavirus in head and neck squamous cell carcinomas.
Guang-Xue CUI ; Xiao-Lei GAO ; Xin-Hua LIANG
West China Journal of Stomatology 2018;36(5):544-551
Human papillomavirus (HPV) is a major causative agent of head and neck squamous cell carcinomas (HNSCC). Over the past several decades, an increasing number of studies established the strong association of HPV with the invasion and metastasis of HNSCC. In the present study, we reviewed the gene mutations in HPV-associated HNSCC and the unique mechanism of E6- and E7-mediated carcinogenesis via interactions with an array of cellular elements. We further discussed the progress in the mechanisms of invasion and metastasis; these mechanisms include non-coding RNAs, deregulating cellular energetics, tumor microenvironment, cancer stem cells, angiogenesis, and lymphangiogenesis.
Head and Neck Neoplasms
;
pathology
;
virology
;
Humans
;
Neoplasm Invasiveness
;
Neoplasm Metastasis
;
Papillomaviridae
;
Papillomavirus Infections
;
Squamous Cell Carcinoma of Head and Neck
;
pathology
;
virology
10.Experimental study on cetuximab and dendritic cells killing head and neck squamous cell.
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(15):1133-1136
OBJECTIVE:
This research aims to investaigate the effect of cetuximab and dendritic cells (DCs) to kill the head and neck squamous cell (HNSCC), in order to provide a new way for the patients of HNSCC.
METHOD:
DCs were induced from peripheral blood monocytes by rhIL-4, rhGM-CSF and TNF-alpha in vitro, 7days later, detecting the surface marks of DCs for example CD83, CD86, and then using MTT and flow cytometry detecting the effect T lymphocytes induced by DCs combining cetuximab to kill HNSCC; EGFR and pEGFR in each group were anlysised by Western blot.
RESULT:
It is successful to induce DCs in vitro. Mature DCs (mDCs) expressed the suface mark such as CD83, CD86 higher compared with immature DCs (imDCs). Compared with other groups, cetuximab combined with DCs significantly enhanced the cytotoxicty and apoptosis to HNSCC (P < 0.05). pEGFR were gradually reduced as the concenetration of cetuximab increasing (P < 0.05). However, comparing with the group of cetuximab, the group of cetuximab combined with DC has no significant difference at the same concentration of cetuximab. In each group EGFR also has no significant diference (P > 0.05).
CONCLUSION
Cetuximab and DCs have synergistic effects, which can significantly enhance the killing effect of HNSCC.
Antibodies, Monoclonal, Humanized
;
pharmacology
;
Apoptosis
;
drug effects
;
Carcinoma, Squamous Cell
;
pathology
;
Cetuximab
;
Dendritic Cells
;
immunology
;
Head and Neck Neoplasms
;
pathology
;
Humans
;
Squamous Cell Carcinoma of Head and Neck
;
Tumor Cells, Cultured