No abstract available.
Japan* ; Sporothrix* ; Sporotrichosis*

No abstract available.
Japan* ; Sporothrix* ; Sporotrichosis*

The present study was designed to obtain omparative data on in vitro antifungal activities of imidazole derivatives. Minimum inhibitory oncentrations of clotrimazole, miconazole, econazole, ketoconazlole and griseofulvin on 4 strains of Trichophyton mentagrophytes, 3 strains of Trichophyton rubrum, 2 strains of Microsporum canis and ] strain of Sporothriv: schenckii were etermined after 3 week' incubation at room temperature on Sabouraud's dextrose liquid media. In addition, the fungicidal activities of miconazole and econazole were tested against Z'richophyton mentagrophytes and Microsporum canis, using the techniques described by Vanbreuseghern(1967) The results are summarzed as follows: ] In most of the dermatophytes studied, 1 to 10 pg/ml of M1C were detected. Diverse susceptibility pattern was observed among different fungal species, but no or minor variability was noted within the same species. The susceptibility of Z'ri- chophyton rubrum showed at MIC of 0. 01 to 10 pg/ml, T ichophyton mentagro- phyt.es and Mic osporum canis at 0.1 to 10 pg/ml and 0. 1 to 1000 gg/ml respec- tively. The Trichophyton rubrum was the most sensitive. In the susceptibility test of Sporothrix schenckii, the high resistance to clotrimazole and griseofuhin was observed. The fungistatic activities of miconazole, econazole and ketoconazole were observed only at concentrations higher than JpQ pg/ml.
Arthrodermataceae ; Clotrimazole ; Econazole ; Glucose ; Griseofulvin ; Ketoconazole ; Miconazole ; Microsporum ; Sporothrix ; Trichophyton

Sporotrichosis is an infection of worldwide distribution caused by the fungal organiam sporotrichum schenckii. The disease is usually of the primary cutaneous variety with involvement of regionaI lymphatics, although dissemination may occur. A case of lymphatic type of Syorotrichosis affecting 33 years old, barber, who has been suffering from painless multiple nodules on right hand 4 forearm, is presented. The skin lesions developed about 3months ago, and increased sise and numbers of the lesion insidiously. Diagnosis was confirmed by characterietic clinical, mycological, histopathological studies He was treated with potassium iodide and there was markedly improved.
Adult ; Diagnosis ; Forearm ; Hand ; Humans ; Potassium Iodide ; Skin ; Sporothrix ; Sporotrichosis*

A case of sporotrichosis, developed on left upper eyelid in 2 weeks after double eyelid operation, was presented. Eruptions were distributed along the operation site and similar to pyoderma or keloid. Painless subcutaneous nodule appeared near the lateral angle of eye in 8 months after onset. Sporotrichum schenckii was cultivated from the tissue fluid of the lesion. PAS positive spores were found extra-cellularly and in histiocytes and multi-nucleated giant cells.
Eyelids* ; Giant Cells ; Histiocytes ; Keloid ; Pyoderma ; Spores ; Sporothrix ; Sporotrichosis*

BACKGROUND: Despite concern about treatment of sporotrichosis, little is known about the sensitivity of S. schenckii against antifungal agents. OBJECTIVE: The purpose of the study was to investigate minimal irihibitory concentration(MIC) of antifungal agents against S.chenckii. METHOD: The tested strains were seven in number. The tested antifngal agents were ketoconazole, itraconazole, amphotericin B, and potassium iodide. A various concentration of drugs were mixed into fluid media containing 50% ethanol, dimethyl sulfoxide, and distilled water. The inoculum consisted of 3mm block containing hyphae and spores. RESULTS: MIC ranges of ket.oconazole, itraconazole, amphotericin B, and potassium iodide were 0.39- 25pg/ml, 0.78-25pg/ml, 0.78-25pg/ml, and 75-550pg/ml, respectively. CONCLUSION: We find that ketoconazole is the most active drug against S. schenckii.
Amphotericin B ; Antifungal Agents* ; Dimethyl Sulfoxide ; Ethanol ; Hyphae ; Itraconazole ; Ketoconazole ; Potassium Iodide ; Spores ; Sporothrix* ; Sporotrichosis ; Water

We report a case of fixed cutaneous type sporotrichosis with atypical histopathologic findings in a 18-year-old male patient, who presented with a 1-year history of a 4x3 cm sized, crusted, erythematous plaque and several small erythematous papules on the left knee. The histopathologic findings showed pseudoepitheliomatous hyperplasia, abscess formation, chronic granulomatous inflammation and many PAS-positive fungal spores and hyphae in the dermis. Sporothrix schenckii was identified with fungal culture of biopsy specimen. The lesions improved with three pulses of itraconazole 400mg daily for a week per month.
Abscess ; Adolescent ; Biopsy ; Dermis ; Humans ; Hyperplasia ; Hyphae ; Inflammation ; Itraconazole ; Knee ; Male ; Spores, Fungal ; Sporothrix ; Sporotrichosis*

BACKGROUND: Sporotrichosis is frequently difficult to diagnose because its morphologic appearance may closely resemble mycobacterial infection or noninfectious inflammatory reaction. Sporothrix schenckii may be difficult to identify in histological sections stained with histochemical staining such as haematoxylin and eosin, periodic-acid-Shiff(PAS) and Gomori's-methenamine-silver (GMS). OBJECTIVE: The purpose of this study was to determine whether immunohistochemical staining with a polyclonal anti-Mycobacterium bovis(BCG) antibody, which is known for its interspecies cross-reactivity between microorganisms, is the suitable screening method to detect Sporothrix schenckii of sporotrichosis in skin tissues. METHODS: Thirty sporotrichosis samples of formalin-fixed, paraffin-embedded skin tissues were stained with anti-BCG immunohistochemical stain (anti-BCG stain) and PAS stain. RESULTS: Thirteen cases (43%) of 30 were positive with PAS stain, and 19 cases (63%) were positive with anti-BCG stain. In 24 cases which were positive in fungal culture, 11 were positive in both stains, 1 was positive in PAS stain only, 6 cases were positive in anti-BCG stain only, and 6 were negative in both stains. In 6 cases which were negative in fungal culture, 1 was positive in both stains, 1 was positive in anti-BCG stain, and 4 were negative in both stains. CONCLUSION: Because of its cross-reactivity with fungi as well as its high sensitivity and minimal background staining, the anti-BCG stain can be the useful screening method of detection of S. schenckii in paraffin-embedded specimens of sporotrichosis.
Coloring Agents ; Eosine Yellowish-(YS) ; Fungi ; Mass Screening* ; Skin ; Sporothrix* ; Sporotrichosis

BACKGROUND: Sporotrichosis is a chronic, deep fungal infection caused by a dimorphic fungus, Sporothrix schenckii. It is widely variable in incidence, distribution and clinical pattern. Clinical manifestations are greatly variable, with important involvement of the skin and the superficial lymphatic system. Although sporotrichosis is the most common deep fungal infection in Korea, few reports have included a long term investigation and survey of sporotrichosis. OBJECTIVE: We investigated patients' records for 42 years to identify the prevalence, epidemiological features and changes in clinical findings on sporotrichosis. METHODS: We carried out a retrospective analysis of 118 patients with sporotrichosis for 42 years (1997~2009) by reviewing the medical records and making phone calls to patients if needed. RESULTS: The incidence of sporotrichosis is on a decreasing tendency at this time and incidents were more common for rural residents (80 cases, 67.8%) than urban residents (38 cases, 32.2%). Lymphocutaneous type (86 cases, 73%) is more common than fixed cutaneous type (32 cases, 27%) and the most frequent occupation of patients was farmer (71 cases, 60%). Seasonally, 42 cases (36%) occurred in winter (December to February), showing higher incidency than any other season. The most common site of the skin lesion was right upper extremity. CONCLUSION: We found considerable changes in prevalence, seasonal distribution and clinical findings of sporotrichosis over 42 years.
Fungi ; Humans ; Incidence ; Korea ; Lymphatic System ; Medical Records ; Occupations ; Prevalence ; Retrospective Studies ; Seasons ; Skin ; Sporothrix ; Sporotrichosis

We report a case of fixed-type sporotrichosis in a 12-month-old infant, who presented with a well-defined scaly erythematous patch on the right lateral nasal root area of approximately 2.5 months duration. Hiopsy revealed pseudoepitheliomatous hyperplasia with chronic granulomatous inflammation in dermis and PAS-positive fungal spores in the stroma. Sporothrix schenckii was identified with fungal culture of biopsy specimen. The lesion was responded to treatment. with itraconazole(3mg/kg) for 12 weeks, without relapse at 5-month follow-up.
Biopsy ; Dermis ; Follow-Up Studies ; Humans ; Hyperplasia ; Infant ; Inflammation ; Itraconazole ; Recurrence ; Spores, Fungal ; Sporothrix ; Sporotrichosis*