1.Protective effects of pretreatment with neurotrophin-3 on intrathecal ropivacaine in rats.
Zhihua SUN ; Qulian GUO ; Xiaoping XU ; Zhong ZHANG ; Na WANG ; Zongbin SONG
Journal of Central South University(Medical Sciences) 2014;39(1):17-22
OBJECTIVE:
To investigate the effect of pretreatment with neurotrophin-3 (NT-3) on intrathecal ropivacaine in rats.
METHODS:
A total of 144 male Sprague Dawley rats weighing 280-320 g were successfully implanted with microspinal cather following the improved methods of Yaksh. The rats were randomly divided into 4 groups and given saline (Group NS, n=36), 0.5% ropivacaine (Group M, n=36), 1% ropivacaine (Group R, n=36), and ropivacaine+NT-3 (Group T, n=36). The rats received 0.12 mL/ kg body weight of ropivacaine at 0.5% or 1%, or normal saline only, via an implanted intrathecal catheter at 90-min interval for 12 h in Group NS, M, R and T. In the meantime the rats also received NT-3 0.1 mg/kg in group T. On days 1, 3, 5, 7, 14 and 28, we assessed the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL), behavioural change and histopathological damage score changed for possible neuronal injury within the spinal cord.
RESULTS:
Compared with Group NS and Group M, the PWMT and PWTL were significantly higher on 1, 3, 5 d and the histopathological damage score was significantly higher on 1, 3, 5, 7, 14 d in Group R (P<0.05). Compared with Group T, the PWMT and PWTL in Group R were significantly higher on 1, 3, 5 d and histopathological damage score was significantly higher on 5, 7, 14 d (P<0.05).
CONCLUSION
NT-3 pretreatment in mice has obvious protective effect against repeated intrathecal injection of 1% ropivacaine in the spinal nerve.
Amides
;
adverse effects
;
Animals
;
Injections, Spinal
;
Male
;
Neurotrophin 3
;
pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Ropivacaine
;
Spinal Cord
;
drug effects
2.Advances in mechanisms of treatment for spinal cord injury with lithium.
China Journal of Orthopaedics and Traumatology 2015;28(7):679-682
Spinal cord injury is a serious disabling disease caused by a series of internal and external factors in the field of orthopaedics and neuroscience, which is a big problem for doctors all over the world. Lithium has been used to treat dipolar disorder for over 100 years. It has been reported that lithium is benefit for brain neuron. The treatment effect for spinal cord injury gets more and more attention. Researches indicate that lithium is benefit for spinal cord injury by protecting neuron,reducing after-injury inflammation increasing the produce and release of neurotrophins, stimulating neurogenesis, enhancing autophagy and inhibiting apoptosis. This article summaries advances in mechanism of treatment for spinal cord injury with lithium by reviewing and analyzing researches. Therapy combined with lithium has a good application prospect.
Animals
;
Apoptosis
;
drug effects
;
Humans
;
Lithium
;
therapeutic use
;
Neurons
;
cytology
;
drug effects
;
Spinal Cord Injuries
;
drug therapy
;
physiopathology
3.The Effect of Strychnine on Membrane Properties of Spinal Motoneurons in the Cat.
Kyu Chang LEE ; Manfred R KLEE ; Hun Jae LEE
Yonsei Medical Journal 1975;16(2):1-28
Strychnine (Stry.) has been used, as an instrument for studies of experimental epilepsy, though its precise mode of action has remained obscure. One mechanism of action was partially clarified in 1954 ,by the demonstration that subconvulsive doses of Stry. reduce the amplitude of inhibitory postsynaptic potentials (IPSPs) in the cat's spinal motoneurons (MN). Because of the rapid onset of its action and the absence of effects upon monosynaptic excitatory postsynaptic potentials (EPSPs), it was proposed that Stry. competed with some unidentified transmitter for inhibitory receptor sites on the postsynaptic membrane. Electrophoresis of Stry. is known to block the inhibitory effects of glycine, a likely candidate as an inhibitory transmitter on MN in the cat spinal cord. A Stry. resistant inhibition seems to exist not only in the higher portion of the CNS, but also for the spinal MN. Gamma amino butyric acid (GABA) is a candidate for this synaptic transmitter. In Nembutal anesthetized cat, intracellular recording of spinal MN was performed during Stry. induced seizure. To conclude, it can be said that there were no consistant changes in the MN action potential which would reflect an action of Stry. upon MN's membrane properties important to seizure generation. It is still to be resolved whether the increase in polysynaptic EPSP amplitude is due to a Stry. effect upon the membrane properties of excitatory interneurons or to an effect only upon the inhibitory as well as the EPSPs.
Action Potentials/drug effects*
;
Animal
;
Cats
;
Convulsions/chemically induced
;
Female
;
Male
;
Membrane Potentials/drug effects*
;
Motor Neurons/drug effects*
;
Spinal Cord/drug effects*
;
Strychnine/pharmacology*
4.Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field.
Ahmad YAHYAZADEH ; Berrin Zuhal ALTUNKAYNAK
Biomedical and Environmental Sciences 2020;33(8):593-602
Objective:
This study aimed to investigate the effect of exposure to a 900 MHz electromagnetic field (EMF) on the cervical spinal cord (CSC) of rats and the possible protective effect of luteolin (LUT) against CSC tissue damage.
Methods:
Quantitative data were obtained stereological, biochemical, immunohistochemical, and histopathological techniques. We investigated morphometric value, superoxide dismutase (SOD) level, and the expression of high-mobility group box 1 protein molecules, as well as histological changes.
Results:
The total number of motor neurons in the EMF group significantly decreased in comparison with that in the control group ( < 0.05). In the EMF + LUT group, we found a significant increase in the total number of motor neurons compared with that in the EMF group ( < 0.05). SOD enzyme activity in the EMF group significantly increased in comparison with that in the control group ( < 0.05). By contrast, the EMF+LUT group exhibited a decrease in SOD level compared with the EMF group ( < 0.05).
Conclusion
Our results suggested that exposure to EMF could be deleterious to CSC tissues. Furthermore, the protective efficacy of LUT against SC damage might have resulted from the alleviation of oxidative stress caused by EMF.
Animals
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Antioxidants
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pharmacology
;
Electromagnetic Fields
;
adverse effects
;
Luteolin
;
pharmacology
;
Male
;
Rats
;
Rats, Wistar
;
Spinal Cord
;
drug effects
;
radiation effects
5.Effects of the spinal cord intrathecal injected to agmatine on intrathecal morphine analgesia.
Zhong-Ping LAN ; Ya-Hui CHEN ; Nan GU ; Li-Ze XIONG ; Yan-Yuan SUN
Chinese Journal of Applied Physiology 2014;30(3):197-203
Agmatine
;
administration & dosage
;
pharmacology
;
Analgesia
;
methods
;
Animals
;
Injections, Spinal
;
Male
;
Morphine
;
pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Spinal Cord
;
drug effects
6.Spontaneous Intraperitoneal Hemorrhage in a Cervical Spinal Cord Injured Patient with Warfarin Therapy: A Case Report.
Sang Il PARK ; Soo Yon YOON ; Sang Cheol KIM ; Ki Seok NAM ; Eun CHOI ; Yang Gyun LEE
Journal of the Korean Academy of Rehabilitation Medicine 2001;25(3):527-530
Spontaneous intraperitoneal hemorrhage in the patient who has cervical spinal cord injury and been treated by warfarin, is rarely reported. In general, adverse drug reactions of warfarin were reported as bleeding, cutaneous microvascular thrombosis. A 46-year-old C4 tetraplegia (ASIA A) patient had been treated by warfarin for a treatment and prevention of pulmonary embolism after cardiopulmonary resuscitation. Suddenly, the patient complained dyspnea and abdominal distension and we noticed that his hemoglobin count was very much lower than usual. So, we had the patient to take the abdominal CT and find out there was a bleeding in intraperitoneal area. We did angiography trying to find out the bleeding focus but in vain. We concluded that it was the spontaneous intraperitoneal hemorrhage due to warfarin therapy and we finally made an improvement with the conservative treatment.
Angiography
;
Cardiopulmonary Resuscitation
;
Drug-Related Side Effects and Adverse Reactions
;
Dyspnea
;
Hemorrhage*
;
Humans
;
Middle Aged
;
Pulmonary Embolism
;
Quadriplegia
;
Spinal Cord Injuries
;
Spinal Cord*
;
Thrombosis
;
Tomography, X-Ray Computed
;
Warfarin*
7.Effects of Ginkgo biloba extract on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion in rabbits.
Li-Hong FAN ; Kun-Zheng WANG ; Bin CHENG
Chinese Journal of Traumatology 2006;9(2):77-81
OBJECTIVETo study the effects of Ginkgo biloba extract (GBE) on lipid peroxidation and apoptosis after spinal cord ischemia/reperfusion (I/R) in rabbits.
METHODSSpinal cord I/R injury model was established according to the description of Erten et al. A total of 27 New Zealand white rabbits were divided into three groups randomly: a sham group (9 rabbits treated with sham operation but without aortic occlusion), a model group (9 rabbits treated with aortic occlusion and volume-matched saline), and a GBE group (9 rabbits treated with aortic occlusion and Ginaton (100 mg/kg) injected 30 minutes before aortic clamping and at the onset of reperfusion). The neurological outcomes were evaluated at 24 and 48 hours after reperfusion, respectively. The spinal cord malondialdehyde (MDA) level, superoxide dismutase (SOD) were then detected. Neural cell apoptosis was determined by terminal deoxynucleotidyl t-ransferase (TdT)-mediated dUTP-fluorescence nick end labeling (TUNEL) method and the expression of bcl-2 and bax were examined histologically in the spinal cord with immunohistochemistry.
RESULTSI/R produced a significant decrease in neurological scoring. The motor scores of the GBE group were significantly higher than those of the model group at 24 and 48 hours after reperfusion (P<0.05). Compared with the model group, GBE ameliorated the down-regulation of SOD and produced a significant reduction of the MDA level (P<0.01). The positive cells for TUNEL in the model group were much more than those of the GBE group (P<0.01). The bcl-2 was up-regulated after I/R, especially in the GBE group (P<0.01). The up-regulation of bax was greatly diminished by GBE (P<0.01).
CONCLUSIONSGBE has protective effects against spinal cord I/R injury, and the mechanism may be that it can scavenge oxygen free radicals and inhibit the apoptosis of neural cells.
Animals ; Apoptosis ; drug effects ; Ginkgo biloba ; Lipid Peroxidation ; drug effects ; Malondialdehyde ; analysis ; Neuroprotective Agents ; therapeutic use ; Phytotherapy ; Plant Extracts ; therapeutic use ; Rabbits ; Reperfusion ; Spinal Cord ; drug effects ; pathology ; Spinal Cord Ischemia ; metabolism ; pathology ; Superoxide Dismutase ; analysis
8.Ethanol reduces neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
Guo YIN ; Fang YAO ; Xiaohui CHEN ; Nan WANG ; Huili WANG ; Hong-En CHANG ; Zhaohu YUAN ; Bingyi WU
Journal of Southern Medical University 2014;34(11):1555-1561
OBJECTIVETo investigate the influence of exposure to different concentrations of ethanol on neural progenitor cells and the differentiation of neurons and glial cells in zebrafish embryos.
METHODSZebrafish embryos were exposed to 1%, 2%, and 2.5% (V/V) ethanol at 5 hpf by adding ethanol to the egg water. In situ hybridization and real-time PCR were used to detect the changes in the mRNA expression profiles of the markers of different cells to examine the effects of alcohol on neural development.
RESULTSThe number of neural precursor cells, neurons and mature glial cells was significantly reduced in the zebrafish embryos following ethanol exposure, and this reduction became more prominent as the ethanol concentration increased. The expression of the early glial marker slc1a3a was down-regulated in the spinal cord but increased in the brain after exposure to increased ethanol concentrations. The expression of the mature glial markers was significantly lowered in response to exposure to increasing ethanol concentrations.
CONCLUSIONSEthanol can reduce neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
Animals ; Brain ; Cell Differentiation ; drug effects ; Embryo, Nonmammalian ; drug effects ; Ethanol ; adverse effects ; Neural Stem Cells ; drug effects ; Neurogenesis ; drug effects ; Neuroglia ; drug effects ; Neurons ; drug effects ; Spinal Cord ; Zebrafish ; embryology
9.Effect of M8046 on expression of COX-2/PGE2 in spinal cord and DRG in rats with neuropathic pain.
Guo-Kun OU ; Rui-Xian WANG ; Jia-Jia LI ; Hong CAO ; Qing-Quan LIAN ; Jun LI
Chinese Journal of Applied Physiology 2013;29(2):97-105
OBJECTIVETo investigate the effects of glucocorticoid receptor antagonist-M8046 on the behavior and the cyclooxygenase-2/prostaglandin E2( COX-2/PGE2) expression in spinal cord dorsal horn and dorsal root ganglia (DRG) in chronic constrictive injury (CCI) rats.
METHODSOne hundred and forty-four male SD rats were randomly divided into 4 groups, 36 rats in each group: Sham operation group (Sham), chronic constrictive group (CCI), M8046 treated group (M8046) and solvent controlled group (Sc). M8046 3 mg/(kg x d) intraperitoneal injection was given after operation in group M8046. Paw thennal withdrawal (PTWL) and paw mechanical withdrawal threshold (PMWT) of rats were measured on 2 pre-operative and 1, 3, 7, 10, 14 post-operative days. The spinal cord and L15 DRG of the operated side was removed at 3, 7, 14 days after surgery. The change of COX-2 and PGE2 expression was determined by immunohistochemical staining and ELISA separately.
RESULTSPTWL and PMWT in CCI group were significantly lower than those in Sham group on every post-operative day (P < 0.05). PTWL and PMWT in M8046 group were significantly higher than those in CCI group on 7, 10, 14 post-operative day (P < 0.05). In spinal dorsal horn, the level of COX-2 and PGE2 expression in CCI group was significantly higher than that in Sham group (P < 0.05). M8046 could significantly attenuate the activation of COX-2 and PGE2 induced by CCI (P < 0.05). The expression of COX-2 and PGE2 in DRG was similar to that in spinal dorsal horn.
CONCLUSIONThe effects of M8046 ameliorate the CCI-induced neuropathic pain may be related to attenuate the expression of COX-2 and PGE2 in spinal cord and DRG.
Animals ; Cyclooxygenase 2 ; metabolism ; Dinoprostone ; metabolism ; Ganglia, Spinal ; drug effects ; metabolism ; Male ; Neuralgia ; metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucocorticoid ; antagonists & inhibitors ; Spinal Cord ; drug effects ; metabolism
10.Neural toxicity induced by accidental intrathecal vincristine administration.
Eun Kyung KWACK ; Dong Ja KIM ; Tae In PARK ; Kab Rae CHO ; Il Hoon KWON ; Yoon Kyung SOHN
Journal of Korean Medical Science 1999;14(6):688-692
Described here is a case of accidental intrathecal administration of vincristine with pathologic findings in the central nervous system. A 3-year-old boy with acute lymphoblastic leukemia, was given his ninth course chemotherapy. Vincristine was accidentally injected intrathecally. The clinical course was rapidly progressive (6-day course) and resulted in death. An autopsy was done. The brain and spinal cord was grossly edematous and congested without any specific feature. Histologically, profound loss of neuron was noted in the spinal cord. Remaining neurons in the spinal cord, particularly anterior horn cells were markedly swollen. The spinal nerves show diffuse axonal degeneration and myelin loss. The upstream portion of the spinal cord (brain stem, cerebellum, cerebrum) showed patchy loss of neurons, especially Purkinje cells and granular cells of the cerebellar cortex. Many neurons showed axonal reaction (chromatolysis) with swelling. Several neurons show intracytoplasmic eosinophilic inclusion body. Myelin loss, axonal swelling and enlargement of perivascular spaces were seen throughout the white matter of central nervous system.
Antineoplastic Agents/therapeutic use*
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Brain/pathology
;
Brain/drug effects
;
Case Report
;
Central Nervous System/pathology
;
Central Nervous System/drug effects*
;
Child, Preschool
;
Fatal Outcome
;
Histocytochemistry
;
Human
;
Injections, Spinal
;
Leukemia, Lymphocytic, Acute/drug therapy*
;
Male
;
Medication Errors*
;
Spinal Cord/pathology
;
Spinal Cord/drug effects
;
Spinal Nerves/pathology
;
Spinal Nerves/drug effects
;
Vincristine/therapeutic use*
;
Vincristine/administration & dosage