1.300 cycles of sperm retrieval from the epididymis and the testis for the intracytoplasmic sperm injection
Journal of Medical and Pharmaceutical Information 2004;0(7):24-30
In this cross-sectional retrospective study, 300 cycles of sperm retrieval from the epididymis and the testis on 213 patients for the intracytoplasmic sperm injection at the Infertility Department of Tu Du Hospital from March 2003 to May 2004 were examined. The results showed that: 100% cycles with embryos and the pregnancy rate of 47.9%; there is no complication except a mild scrotal discomfort lasting a few days after open surgery; percutaneous epididymal sperm aspiration (PESA) was the most performed retrieval technique (61%), which was easy-done, quickly-performed, safe and effective
Sperm Injections, Intracytoplasmic
;
Sperm Retrieval
;
Epididymis
;
Testis
2.The first Vietnamese baby born from intracytoplasmic sperm injection using testicular sperm from impaired spermatogenesis testes
Ho Chi Minh city Medical Association 2004;9(2):106-109
The first Viet Nam baby born from intracytoplasmic sperm injection using testicular sperm from impaired spermatogenesis testes in 1/3/2004. There was approximately 50000 sperm after preparation, among them 20% was motile. From 12 retrieved oocytes, 11 were mature and therefore injected. Five oocytes fertilized and developed into 4 embryos. Embryo transfer was carried out for all 4 embryos. One embryo implanted and kept growing till the 37th week of pregnancy. Patient underwent ceasarian section
Spermatogenesis
;
Sperm Retrieval
;
Sperm Injections, Intracytoplasmic
3.Microscopic sperm aspiration and in vitro fertilization in obstructive azoospermia.
Cheol BAE ; Jae Yup HONG ; Seung Jae LEE ; Jong Min PARK ; Seong Il NO ; Jong Young JEON
Korean Journal of Urology 1991;32(5):781-787
We performed microscopic sperm aspiration and IVF in 2 cases of distal vas obstruction. 4 cases or epididymal obstruction, 3 cases of congenital vas aplasia. The results were as follows ; 1. The fertilization was successful in 2 cases of distal vas obstruction(100%). 1 case of epididymal obstruction(25%). 1 case of congenital vas. aplasia(33.3%). 2. In 4 cases with Sertoli cell failure. the count and quality of aspirated sperm was lower than in 5 cases with normal hormonal study. The fertilization was successful in 1 case with Sertoli cell failure(25%) and 3 cases with normal hormonal study(60%). In conclusion. microscopic sperm aspiration and IVF proved helpful in treatment of patients with distal vas obstructin, congenital vas aplasia. but the vasoepididymostomy proved more helpful in treatment of patients with epididymal obstruction then microscopic sperm aspiration and IVF until now. When microscopic sperm aspiration and IVF is performed, it seems that in Sertoli cell failure group. there will be lesser fertilization success cases than in normal hormonal study group.
Azoospermia*
;
Fertilization
;
Fertilization in Vitro*
;
Humans
;
Sperm Retrieval*
;
Spermatozoa*
4.Three-step sperm retrieval for 73 non-obstructive azoospermia patients.
Meng MA ; Ping PING ; Jian-Hu WANG ; Peng LI ; Shi YANG ; Jian-Shan ZHU ; Hui LU ; Hong-Liang HU ; Zheng LI
National Journal of Andrology 2012;18(7):606-610
OBJECTIVETo investigate the value and clinical application of the Three-Step Sperm Retrieval method in improving the sperm retrieval rate for non-obstructive azoospermia (NOA) patients.
METHODSSeventy-three NOA patients underwent Three-Step Sperm Retrieval in the following order of procedures: testicular fine needle aspiration (TFNA), testicular sperm extraction (TSE), and microdissection testicular sperm extraction (MD-TSE). The testicular tissue obtained from each step was observed for spermatozoa under the 400-fold inverted microscope. If spermatozoa were found in one step, the operation would be stopped; otherwise, the next step would be carried out. The testicular tissue was subjected to pathological examination.
RESULTSSpermatozoa was found in the testicular tissue in 38.4% of the cases (28/73) at TFNA as the first step, in 52.1% (38/73) at TFNA and TSE, and in 64.4% (47/73) at TFNA, TSE and MD-TSE. Pathological examination showed 25 of the cases to be Sertoli cell-only syndrome, 21 to be sperm maturation arrest and the other 27 to be hypospermatogenesis, in which spermatozoa were found in 10, 14 and 23 cases, respectively.
CONCLUSIONThe Three-Step Sperm Retrieval method can significantly improve the sperm retrieval rate for NOA patients. And the sperm retrieval rate is associated with the pathological type of the testicular tissue, a higher rate with hypospermatogenesis.
Adult ; Azoospermia ; Humans ; Male ; Sperm Retrieval ; Testis ; pathology ; surgery
5.Successful human pregnancy by in-vitro fertilization using sperm retrieved by testicular sperm extraction (TESE): the first reported local experience
Ricero Claudette P. ; Almeda Leonardo A. ; Vera Ma.Trinidad R. ; Torres Romerico F.
Philippine Journal of Reproductive Endocrinology and Infertility 2008;5(1):23-31
A 31 year old Filipino male diagnosed with obstructive azoospermia underwent TESE. Other current advanced sperm retrieval techniques include MESA, PESA and TESA confounded with female factors such as advanced maternal age and polycystic ovaries, IVF-ICSI eventually resulted to a successful pregnacy outcome. Several factors contributing to the success of IVF-ICSI eventually resulted to a successful pregnancy outcome. Several factors contributing to the success of IVF-ICSI in this case were also discussed. Although assisted reproductive technology is relatively new in our country, it proved to be promising in our local setting.
Human
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Female
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Adult
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REPRODUCTIVE TECHNIQUES, ASSISTED
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AZOOSPERMIA
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SPERM RETRIEVAL
6.Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI).
Taek Hoo LEE ; Hang Jin KIM ; Gun Ho SONG ; Dae Geun KIM ; Sang Sik CHUN ; Yoon Kyu PARK ; Tae Kwang SUH ; Byeong Gyun JEON ; Eun Kyung RYU ; Eun Sook LEE ; Jin Soo MOON ; Kwang Chull KIM
Korean Journal of Obstetrics and Gynecology 1997;40(12):2741-2746
No abstract available.
Blastocyst
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Coculture Techniques
;
Fertilization
;
Oocytes*
;
Sperm Injections, Intracytoplasmic
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Sperm Retrieval*
;
Spermatozoa*
7.The Study on Comparison of Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Epididymal Sperm and Testicular Sperm.
Ki Cheong SUNG ; Moon Joo KANG ; Hee Sun KIM ; Sun Kyung OH ; Seung Yup KU ; Chang Suk SUH ; Seok Hyun KIM ; Young Min CHOI ; Jung Gu KIM ; Shin Yong MOON
Korean Journal of Fertility and Sterility 2003;30(2):119-126
OBJECTIVE: This study was carried out to compare the clinical outcomes of intracytoplasmic sperm injection (ICSI) in patients with obstructive azoospermia according to sperm retrieval site and technique; microsurgical epididymal sperm aspiration (MESA), percutaneous epididymal sperm aspiration (PESA), testicular sperm extraction by open biopsy (TESE). METHODS: The outcomes of ICSI and IVF-ET were evaluated and compared among 3 groups. Seventy three men suffering from infertility due to obstructive azoospermia had 107 ICSI cycles using MESA (21 cycles in 15 patients), PESA (26 cycles in 17 patients) and TESE (60 cycles in 41 patients). RESULTS: In the clinical outcomes in patients undergoing ICSI with epididymal or testicular sperm, there were no significant differences in fertilization rate (66.1% vs. 60.5%), cleavage rate (94.9% vs. 97.6%), cumulative embryo score (CES) (51.3 vs. 58.8), implantation rate (7.9% vs. 6.1), and clinical pregnancy rate per ET (30.4% (14/46) vs. 25.4% (15/59)) between both groups. Also, in the clinical outcomes in ICSI patients using MESA, PESA, TESE, there were no significant differences in fertilization rate (61.8%, 69.4%, 60.5%), cleavage rate (92.1%, 97.3%, 97.6%), CES (38.1, 52.0, 58.8), implantation rate (9.5%, 6.6%, 6.1%), and clinical pregnancy rate per ET (35% (7/20), 26.9% (7/26), 25.4% (15/59)) among 3 groups. CONCLUSION: When compared with MESA or TESE, PESA, the clinical outcomes were similar in ICSI patients with obstructive azoospermia whatever the origin or the technique of sperm retrieval. However, we considered PESA is more time-saving and cost effective for ICSI in patients with obstructive azoospermia.
Azoospermia
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Biopsy
;
Embryonic Structures
;
Fertilization
;
Humans
;
Infertility
;
Male
;
Pregnancy Rate
;
Sperm Injections, Intracytoplasmic*
;
Sperm Retrieval
;
Spermatozoa*
8.The Study on Comparison of Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Epididymal Sperm and Testicular Sperm.
Ki Cheong SUNG ; Moon Joo KANG ; Hee Sun KIM ; Sun Kyung OH ; Seung Yup KU ; Chang Suk SUH ; Seok Hyun KIM ; Young Min CHOI ; Jung Gu KIM ; Shin Yong MOON
Korean Journal of Fertility and Sterility 2003;30(2):119-126
OBJECTIVE: This study was carried out to compare the clinical outcomes of intracytoplasmic sperm injection (ICSI) in patients with obstructive azoospermia according to sperm retrieval site and technique; microsurgical epididymal sperm aspiration (MESA), percutaneous epididymal sperm aspiration (PESA), testicular sperm extraction by open biopsy (TESE). METHODS: The outcomes of ICSI and IVF-ET were evaluated and compared among 3 groups. Seventy three men suffering from infertility due to obstructive azoospermia had 107 ICSI cycles using MESA (21 cycles in 15 patients), PESA (26 cycles in 17 patients) and TESE (60 cycles in 41 patients). RESULTS: In the clinical outcomes in patients undergoing ICSI with epididymal or testicular sperm, there were no significant differences in fertilization rate (66.1% vs. 60.5%), cleavage rate (94.9% vs. 97.6%), cumulative embryo score (CES) (51.3 vs. 58.8), implantation rate (7.9% vs. 6.1), and clinical pregnancy rate per ET (30.4% (14/46) vs. 25.4% (15/59)) between both groups. Also, in the clinical outcomes in ICSI patients using MESA, PESA, TESE, there were no significant differences in fertilization rate (61.8%, 69.4%, 60.5%), cleavage rate (92.1%, 97.3%, 97.6%), CES (38.1, 52.0, 58.8), implantation rate (9.5%, 6.6%, 6.1%), and clinical pregnancy rate per ET (35% (7/20), 26.9% (7/26), 25.4% (15/59)) among 3 groups. CONCLUSION: When compared with MESA or TESE, PESA, the clinical outcomes were similar in ICSI patients with obstructive azoospermia whatever the origin or the technique of sperm retrieval. However, we considered PESA is more time-saving and cost effective for ICSI in patients with obstructive azoospermia.
Azoospermia
;
Biopsy
;
Embryonic Structures
;
Fertilization
;
Humans
;
Infertility
;
Male
;
Pregnancy Rate
;
Sperm Injections, Intracytoplasmic*
;
Sperm Retrieval
;
Spermatozoa*
9.The value of histopathologic examination of testis tissue from testicular sperm aspiration.
Shen-Min YANG ; Bing CHEN ; Yi-Chao SHI ; Duan-Gai WEN ; Hong LI ; Wei WANG ; Hong-Bo CHENG
National Journal of Andrology 2013;19(10):899-901
OBJECTIVETo evaluate histopathologic examination of the testis tissue from testicular sperm aspiration (TESA).
METHODSWe analyzed the results of inverted microscopy and histopathologic examination of 96 samples of testis tissue from TESA, and compared the accuracy of the two methods in detecting sperm in the testis tissue.
RESULTSAmong the 11 cases in which sperm was found by inverted microscopy, 9 were confirmed by histopathologic examination, and among the 57 cases in which sperm was not detected by inverted microscopy, 11 (19.3%) were found with sperm by histopathologic examination. Histopathologically, the cases in which sperm was not found by inverted microscopy included Sertoli-cell-only syndrome (n = 34), maturation arrest (n = 12) and hypospermatogenesis (n = 11).
CONCLUSIONHistopathologic examination may reveal sperm in the TESA testis tissue proved to be sperm-absent by microscopy, and thus offer valuable information for a second testicular sperm retrieval.
Adult ; Azoospermia ; pathology ; Humans ; Male ; Middle Aged ; Sperm Count ; Sperm Retrieval ; Testis ; pathology ; Young Adult
10.Influence of sperm from different sources on the clinical outcome of intracytoplasmic sperm injection.
Cheng SONG ; Yong ZENG ; Xiao-dong HU ; Jin-quan CHENG ; Ju HUANG ; Li-shi HUANG ; Wei ZHANG
National Journal of Andrology 2009;15(9):822-824
OBJECTIVETo analyze the clinical outcomes of intracytoplasmic sperm injection (ICSI) with spermatozoa from different sources.
METHODSWe retrospectively studied the rates of fertilization, clinical pregnancy, implantation, abortion, ectopic pregnancy and delivery in 682 patients treated by ICSI, who were divided according to the sperm sources into an ejaculated sperm group (ES, n = 598), a percutaneous epididymal sperm aspiration (PESA, n = 58) and a testicular sperm extraction (TSE, n = 26).
RESULTSThe fertilization rate was significantly lower in the TSE than in the ES and PESA groups (81.06% vs 87.95% and 87.82%, P < 0.05). But no statistically significant differences were observed among the ES, PESA and TSE groups in the rates of clinical pregnancy (39.46%, 48.28% and 34.62%), implantation (19.80%, 23.80% and 18.34%), abortion (13.13%, 17.86% and 11.11%), ectopic pregnancy (5.51%, 7.14% and 11.11%) and delivery (32.11%, 36.21% and 26.92%) (P > 0.05).
CONCLUSIONAlthough TSE-obtained sperm affect the rate of fertilization, those obtained by TSE and PESA do not obviously influence the outcome of clinical pregnancy.
Adult ; Female ; Humans ; Male ; Pregnancy ; Pregnancy Outcome ; Retrospective Studies ; Sperm Injections, Intracytoplasmic ; methods ; Sperm Retrieval ; Spermatozoa