1.Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus.
Xiaoyu DENG ; Yanlong CONG ; Renfu YIN ; Guilian YANG ; Chan DING ; Shengqing YU ; Xiufan LIU ; Chunfeng WANG ; Zhuang DING
Journal of Veterinary Science 2014;15(4):511-517
Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two-dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass spectrometer including three that were down-regulated and 10 that were up-regulated. These proteins were sorted into five groups based on function: macromolecular biosynthesis, cytoskeleton organization, metabolism, stress responses, and signal transduction. Furthermore, Western blot analysis confirmed the down-regulation of integrin-linked kinase expression and up-regulation of lamin A production. These data provide insight into the in vivo response of target cells to NDV infection at the molecular level. Additionally, results from this study have helped elucidate the molecular pathogenesis of NDV and may facilitate the development of new antiviral therapies as well as innovative diagnostic methods.
Animals
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Avian Proteins/*genetics/metabolism
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*Chickens
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*Gene Expression Regulation
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Leukocytes, Mononuclear/enzymology/virology
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Newcastle Disease/*genetics/virology
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Newcastle disease virus/*physiology
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Poultry Diseases/*genetics/virology
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*Proteome
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Specific Pathogen-Free Organisms
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary
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Tandem Mass Spectrometry/veterinary
2.Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs.
Paola GANDOLFI-DECRISTOPHORIS ; Gertraud REGULA ; Orlando PETRINI ; Jakob ZINSSTAG ; Esther SCHELLING
Journal of Veterinary Science 2013;14(4):449-456
We investigated the distribution of commensal staphylococcal species and determined the prevalence of multi-drug resistance in healthy cats and dogs. Risk factors associated with the carriage of multi-drug resistant strains were explored. Isolates from 256 dogs and 277 cats were identified at the species level using matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The diversity of coagulase-negative Staphylococci (CNS) was high, with 22 species in dogs and 24 in cats. Multi-drug resistance was frequent (17%) and not always associated with the presence of the mecA gene. A stay in a veterinary clinic in the last year was associated with an increased risk of colonisation by multi-drug resistant Staphylococci (OR = 2.4, 95% CI: 1.1~5.2, p value LRT = 0.04). When identifying efficient control strategies against antibiotic resistance, the presence of mechanisms other than methicillin resistance and the possible role of CNS in the spread of resistance determinants should be considered.
Animals
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Anti-Bacterial Agents/*pharmacology
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Bacterial Proteins/genetics/metabolism
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Cat Diseases/epidemiology/*microbiology
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Cats
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Coagulase/genetics/metabolism
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Dog Diseases/epidemiology/*microbiology
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Dogs
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*Drug Resistance, Multiple, Bacterial
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Female
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Male
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Prevalence
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Risk Factors
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Seasons
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary
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Staphylococcal Infections/epidemiology/microbiology/*veterinary
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Staphylococcus/classification/genetics/*isolation & purification
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Switzerland/epidemiology