Procedure of a bioelectric signal collection system for vivo critter is introduced in this paper. It is easy to measure the bioimpedance in the tip of appliance, when puncture into the tissue, especially puncture into the bone tissue. We can get a judgment on the position of appliance, thereby achieve assistance on the clinic operation.
Biopsy, Needle ; instrumentation ; Bone and Bones ; physiology ; Electrophysiological Phenomena ; Equipment Design ; Specimen Handling ; instrumentation

Biopsy, Needle ; Genetic Variation ; Hepatitis B, Chronic ; pathology ; Humans ; Liver ; pathology ; Specimen Handling

Biopsy, Needle ; methods ; China ; Humans ; Kidney ; pathology ; Kidney Diseases ; pathology ; Kidney Neoplasms ; pathology ; Specimen Handling

Animals ; Biopsy, Needle ; Humans ; Kidney ; ultrastructure ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron, Transmission ; methods ; Specimen Handling

Background: Term prelabor rupture of membranes (PROM) increases the risk of maternal and neonatal infections. Objective: To compare rates of positive bacterial growth in placental swab cultures done among women who received ampicillin prophylaxis at different timings after term PROM. Design: Matched cohort study. Setting: Department of Obstetrics and Gynecology at Southern Philippines Medical Center in Davao City, Philippines. Participants: 120 pregnant women aged ≥18 years old, at ≥37 weeks age of gestation, with PROM: 40 women received ampicillin within 6 hours (6H group), 40 within >6 to 12 hours (12H group), and 40 within >12 to 18 hours (18H group) of onset of PROM. Main outcome measures Rates of positive bacterial growth in postpartum placental swab cultures; most common bacterial isolates; and signs of intraamniotic infection (IAI).
Ampicillin ; Specimen Handling

INTRODUCTIONIdentifying malignancy either preoperatively or intraoperatively can have a significant impact on the management of salivary gland tumours. We review our experience with fine needle aspiration cytology (FNAC) and frozen section (FS) for salivary gland lesions. We analyse the accuracy of both modalities and their influence on management.

MATERIALS AND METHODSRetrospective review of 114 patients who underwent salivary gland surgery, 91 with intraoperative FS and 68 with preoperative FNAC. Both sets of results were compared against each other and the final histopathological diagnosis.

RESULTSThe accuracy of FS was 92.3%, with a sensitivity and specificity of 62.5% and 100%. Histologic concordance was 92.4% for benign lesions, and 100% for malignant tumours. The accuracy of FNAC was 89.7%, with a sensitivity and specificity of 100%. The non-diagnostic rate was 10.3%. Histologic concordance for FNAC was inferior to that for FS, with only 64.2% of benign lesions and 50% of malignant tumours correctly identified. FNAC did not alter the management of benign disease even when a correct diagnosis was obtained.

CONCLUSIONOur results suggest that FNAC and FS are complementary in usefulness for malignant tumours. However, FNAC does not influence the management of benign lesions and routine FNAC for every patient may not be cost-effective.

Biopsy, Fine-Needle ; standards ; Frozen Sections ; Humans ; Intraoperative Care ; Retrospective Studies ; Salivary Gland Neoplasms ; diagnosis ; pathology ; Salivary Glands ; pathology ; Sensitivity and Specificity ; Specimen Handling

BACKGROUND: Accurate measurement of blood glucose concentrations is essential for defining diabetes, and the minimization of ex vivo glycolysis has been recommended. Recent guidelines advocate two kinds of methods for sample collection and processing: either the sodium fluoride (NaF) method or immediate refrigeration using a serum separation tube (SST). We investigated the difference between the two methods in measuring subsequent glucose concentrations using blood specimens from participants recruited for the fourth Korean National Health and Nutrition Examination Survey. METHODS: Paired venous blood samples were collected in an SST and a NaF tube from 1,103 men and women. SST serum was separated within 30 min, including standing for 15 min, and then refrigerated. The NaF samples were refrigerated, but not separated until immediately before analysis. We compared the blood glucose concentrations between the SST (SST glucose) and NaF (NaF glucose) methods. RESULTS: The mean SST glucose was significantly higher than NaF glucose (99.0 mg/dL vs 96.5 mg/dL, P<0.05). NaF glucose showed a negative mean bias of 2.6 mg/dL vs SST glucose but showed high correlation (R=0.9899). There was no significant correlation between the bias of blood glucose concentrations by two methods and the storage time of NaF glucose. CONCLUSIONS: The negative bias associated with the use of NaF tubes may significantly affect the prevalence of diabetes. Serum separation and refrigeration within 30 min after venous sampling is recommended over NaF method, not only to minimize the preanalytical impact on detecting diabetes but also to reduce sample volume and number of tubes.
Blood Glucose/*analysis ; Blood Specimen Collection/*methods ; Diabetes Mellitus/diagnosis ; Female ; Glycolysis/*drug effects ; Humans ; Male ; Nutrition Surveys ; Republic of Korea ; Sodium Fluoride/*pharmacology ; Specimen Handling

OBJECTIVETo study some factors (temperature, time, anticoagulate, reagent, apparatus) which influence the detection of serum enzyme.

METHODSSerums obtained from same patient are determined in different conditions. Compare the statistical difference among each group.

RESULTSThe anticoagulant-heparinize Li influence the determination of ADA and CH50. If serums were sent in room temperature for 24 hours, the results will be different. Using regents from different factories will receive different results.

CONCLUSIONDifferent conditions and reagents will influence results. To obtain correct data, we must institute and perform standard regulation.

Adenosine Deaminase ; metabolism ; Blood Specimen Collection ; Complement Hemolytic Activity Assay ; Complement System Proteins ; metabolism ; Equipment and Supplies ; Hepatitis ; blood ; enzymology ; Humans ; Indicators and Reagents ; Male ; Serum ; enzymology ; Specimen Handling ; Temperature

No abstract available.
Otitis Media* ; Otitis* ; Specimen Handling*

No abstract available.
Otitis Media* ; Otitis* ; Specimen Handling*