1.Homozygous Deletion of p16INK4 and p15INK4B Genes in Human Advanced Ovarian Carcinoma.
Korean Journal of Obstetrics and Gynecology 2000;43(4):649-658
OBJECTIVE: p16INK4 and p15INK4B genes are known to be tumor suppressor genes which reside in p21 region of chromosome 9 and are related to cell cycle control as an inhibitor of cyclin-dependent-kinase. We designed this study to search for deletion and decreased expression of p16INK4 and p15INK4B genes in advanced ovarian carcinomas. METHODS: Polymerase chain reaction (PCR)-based analysis was performed to search for deletion of p16INK4 and p15INK4B using DNA extracted from frozen tissue in liquid nitrogen of thirty-one advanced ovarian carcinoma patients. The intensities of PCR bands were analyzed using an imaging densitometer to determine gene dosage in tumor samples and the relative gene dosage was calculated by comparing band intesity of p16INK4 or p15INK4B with that of beta-globin gene. Homozygous deletions were assigned to tumors in which the ratio was reduced to less than 25% in any one of exons of p16INK4 and p15INK4B. Immunohistochemical techniques were used to study the expression of p16INK4. p16-negative cells were characterized by the absence of nuclear staining, whereas cytoplasmic staining was variable. Clinico-pathologic features, complete remission rates and survivals were analyzed according to the status of p16INK4 and p15INK4B genes. RESULTS: Homozygous deletion of p16INK4 was detected in 12.9% of advanced ovarian carcinoma patients and that of p15INK4B in 35.5%. Clinico-pathologic features such as FIGO stage, histological grade, serum CA-125 levels were not different from groups with homozygously deleted p16INK4 and p15INK4B to those with normal genes. The survival of patients (13 [6-20] months) with homozygously deleted p16INK4 was significantly shorter than that (30 [8-52] months) of patients with normal p16INK4 (p=0.046; Log-rank test). CONCLUSION: These observations indicate that deletions of p16INK4 and p15INK4B gene might be involved in tumorigenesis of ovarian carcinoma and could be useful as a prognostic factor. A prospective, controlled study with more patients will be mandatory in the future.
beta-Globins
;
Carcinogenesis
;
Cell Cycle Checkpoints
;
Chromosomes, Human, Pair 9
;
Cytoplasm
;
DNA
;
Exons
;
Gene Dosage
;
Genes, Tumor Suppressor
;
Humans*
;
Immunohistochemistry
;
Nitrogen
;
Polymerase Chain Reaction
2.Infrequent expression of ras in genital neoplasms initiated by 7,12- dimethylbenzanthracene, and promoted by 12-0-term decanoyl phorbol- 13-acetate in ICR mice.
Soon Beom KANG ; Hae Suk KIM ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1992;35(1):36-45
No abstract available.
Animals
;
Mice
;
Mice, Inbred ICR*
3.Hydrochlorothiazide-induced phototoxic reaction.
Jin Soon KANG ; Tae Heung KIM ; Ki Beom PARK
Korean Journal of Dermatology 1992;30(4):529-534
A 49-year-old male had erythematous to rusky red papules, indurated plaques and lichenified patches with hyperpigmentation on sun-exposed areas for 6 years. Phototest revealed the decreased rninimal erythemal dose to UVA(10J/cm. Photopatch test with 5% Trandate ointment, 5% hydrochlorthiazide ointment and vaselin. as a control were all negative. Two weeks after cessation of Trandate, an oral challenge of hydrochlorthiazide followed by phototest was perfrirmed resulting in exacerbation of skin lesions and photosensitivity with a decreased MED to UVA(10J/cm) again. After the cesation of Trandate containing hydrochlorthiazide, the skin lesions were improved with complete loss of photosensitivity. But, improvement of the infiltrated or licheified plaques were delayed. Presenile cataract previously noted in the patient seemed to be related to his longstanding intake of hydrochlorthiazide.
Cataract
;
Humans
;
Hyperpigmentation
;
Labetalol
;
Male
;
Middle Aged
;
Skin
4.A case of amniotic band syndrome with anencephaly.
Young Chul YOO ; Chung Hoon KIM ; Soon Beom KANG
Korean Journal of Obstetrics and Gynecology 1991;34(5):705-710
No abstract available.
Amniotic Band Syndrome*
;
Anencephaly*
;
Infant, Newborn
6.Air in Vagina: Significance in the Staging of Uterine Cervical Carcinoma.
Byung Ihn CHOI ; Man Chung HAN ; Seung Hyup KIM ; Soon Beom KANG ; Hyo Pyo LEE
Journal of the Korean Radiological Society 1994;30(1):169-173
PURPOSE: To evaluate tlne significance of vaginal air seen on CT scan in preoperative staging of uterine cervical carcinoma. METHODS AND MATERIALS: A comparison was made between CT findings of vaginal air and true vaginal involvement status in 85 patients with uterine cervical carcinoma. CT findings were analyzed in terms of the presence or absence of vaginal air, number of CT slices in which vaginal air was seen, shape of vaginal air, and relation of vaginal airto cervical mass. RESULTS: Vaginal air was present in 35 patients and was absent in 50. Although the mere presence of vaginal air or multiplicity of CT slices showing vaginal air did not signify the presence of vaginal involvement, vaginal air with irregular margin or vaginal air adjacent to uterine cervical mass was suggestive of vaginal involvement. CONCELUSION: These observation of vaginal air in interpreting CT may be helpful in the preoperative staging of uterine cervical carcinoma.
Humans
;
Tomography, X-Ray Computed
;
Vagina*
7.Treatment with EMA-CO regimen in the management of high risk gestational trophoblastic tumor.
Young Min CHOI ; Jin Wan PARK ; Do Yeong HWANG ; Soon Beom KANG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1991;34(1):91-100
No abstract available.
Trophoblastic Neoplasms*
;
Trophoblasts*
8.Modulation of development of 7,12-dimethylbenzanthracene-12-0-tetra decanoyl phorbol-13-acetate induced mouse vulvar, uterine cervical tumors by indomethacin.
Hae Suk KIM ; Wan Young KIM ; Soon Beom KANG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1992;35(1):46-50
No abstract available.
Animals
;
Indomethacin*
;
Mice*
9.CpG island methylation and gynecolgic malignancy.
Yong Tark JEON ; Jae Weon KIM ; Soon Beom KANG
Korean Journal of Gynecologic Oncology 2005;16(1):1-7
No abstract available.
CpG Islands*
;
Methylation*
10.Douglas A. Levine, Richard R. Barakat, Nadeem R. Abu-Rustum, 2008. Atlas of Procedures in Gynecologic Oncology, Second Edition. Informa HealthCare, London, UK. 320 pp. Hardbound, ISBN: 978-0-418-21732-0.
Journal of Gynecologic Oncology 2008;19(4):281-281
No abstract available.
Delivery of Health Care
;
London