BACKGROUND:Adipose-derived mesenchymal stem cells have the ability to self-renew and have pluripotent potential under specific conditions in vitro, which have broad application prospects in clinical practice. However, isolation and culture of adipose-derived mesenchymal stem cells stil appear to have many difficulties and shortcomings. OBJECTIVE:To isolate and culture rabbit adipose-derived mesenchymal stem cells in vitro in order to study their morphology, cellsurface markers and biological properties as wel as to investigate the osteogenic and adipogenic potentials of adipose-derived mesenchymal stem cells in vitro. METHODS:Primary adipose-derived mesenchymal stem cells were isolated from the subcutaneous adipose tissue of posterior cervical region from New Zealand white rabbits and digested by 0.1%col agenase I. The cells were passaged and amplified by the trypsin digestion. The passage 4 adipose-derived mesenchymal stem cells were induced to differentiate after exposure to adipogenic or osteogenic medium. The oil red O staining, alkaline phosphatase and alizarin red staining were used to detect the results. The cellviability was detected by the cellcounting kit 8 method to drawn the growth curve. cellsurface markers were examined using flow cytometry. RESULTS AND CONCLUSION:The adipose-derived mesenchymal stem cells isolated from the subcutaneous adipose tissue of rabbits exhibited a fusiform adherent growth in a vortex pattern, and had a strong capability of proliferation that could be passaged stably to the 10th generation. Flow cytometry results showed that the cells highly expressed CD29, CD90, CD44, but lowly expressed CD45 and CD34. After adipogenic induction, the adipose-derived mesenchymal stem cells were positive for oil red O staining;after osteogenic induction, the cells were both positive for alkaline phosphatase and alizarin red staining. These findings suggest that the adipose-derived mesenchymal stem cells were successful y isolated and cultured from the subcutaneous adipose tissue of rabbits, and these cells are pluripotent with the potential to differentiate into adipocytes and osteoblasts, which are expected to be ideal seed cells for bone tissue engineering.

Purpose: In the Fourth Industrial Revolution, nursing education will change in a different way than before. The purpose of this study was to analyze characteristics of qualitative research on learning experiences for nursing students, and to suggest directions for nursing education geared to the needs of the future. Methods: An integrative review method was used. Based on Whittemore & Knafl’s approach, five steps were applied. Results: Eleven papers met the selection criteria and had above average ratings in quality appraisals. Three characteristics related to the learning experiences of nursing students were derived: (1) overcoming difficulties in the learning process and concentrating on problem-solving, (2) improving self-efficacy through experiencing achievement in the learning process, (3) establishing nursing professionalism, (4) identifying the importance of self-directed and self-reflected learning, and (5) developing teamwork. Conclusion This review found that various learning experiences were conducted for practical experience and learner-oriented learning. Nursing students overcame difficulties to achieve their learning outcomes, and developed their professionalism. Further study is required to comprehensively explore research including other countries, and the experiences of instructors.

Objectives: This study examined the symptoms and emotions expressed by older adults with cancer and their caregivers in South Korean online cancer communities. It aimed to identify narrative patterns and provide insights to inform personalized care strategies. Methods: We analyzed 6,908 user-generated posts collected from major online cancer communities in South Korea. Keyword frequency analysis, term frequency-inverse document frequency, 2-gram analysis, and latent Dirichlet allocation-based topic modeling were applied to explore language patterns. Sentiment analysis identified 12 emotional categories, and Pearson correlation coefficients were calculated to examine associations between symptoms and emotional expressions. All data were cleaned and standardized prior to analysis. Results: Many users expressed anxiety (20.63%) and depression (19.59%), frequently associated with chemotherapy and sleep disturbances. Among reported symptoms, sleep problems carried the highest negative sentiment (79.81%), underscoring their profound impact on well-being. Topic modeling consistently revealed seven recurring themes, including treatment decision-making, symptom management, and concerns about family, demonstrating the layered and personalized experiences of older cancer patients and their caregivers. Conclusions This study explored treatment-related and symptom-related difficulties faced by older adults with cancer. Many reported significant emotional strain, especially anxiety, depression, and sleep disturbances. These findings highlight the necessity for supportive strategies addressing both psychological and physical aspects of care. Future research could investigate the utility of large language models in analyzing these narratives, provided the data is ethically managed and appropriate for such use.

Objectives: This study examined the symptoms and emotions expressed by older adults with cancer and their caregivers in South Korean online cancer communities. It aimed to identify narrative patterns and provide insights to inform personalized care strategies. Methods: We analyzed 6,908 user-generated posts collected from major online cancer communities in South Korea. Keyword frequency analysis, term frequency-inverse document frequency, 2-gram analysis, and latent Dirichlet allocation-based topic modeling were applied to explore language patterns. Sentiment analysis identified 12 emotional categories, and Pearson correlation coefficients were calculated to examine associations between symptoms and emotional expressions. All data were cleaned and standardized prior to analysis. Results: Many users expressed anxiety (20.63%) and depression (19.59%), frequently associated with chemotherapy and sleep disturbances. Among reported symptoms, sleep problems carried the highest negative sentiment (79.81%), underscoring their profound impact on well-being. Topic modeling consistently revealed seven recurring themes, including treatment decision-making, symptom management, and concerns about family, demonstrating the layered and personalized experiences of older cancer patients and their caregivers. Conclusions This study explored treatment-related and symptom-related difficulties faced by older adults with cancer. Many reported significant emotional strain, especially anxiety, depression, and sleep disturbances. These findings highlight the necessity for supportive strategies addressing both psychological and physical aspects of care. Future research could investigate the utility of large language models in analyzing these narratives, provided the data is ethically managed and appropriate for such use.

Objectives: This study examined the symptoms and emotions expressed by older adults with cancer and their caregivers in South Korean online cancer communities. It aimed to identify narrative patterns and provide insights to inform personalized care strategies. Methods: We analyzed 6,908 user-generated posts collected from major online cancer communities in South Korea. Keyword frequency analysis, term frequency-inverse document frequency, 2-gram analysis, and latent Dirichlet allocation-based topic modeling were applied to explore language patterns. Sentiment analysis identified 12 emotional categories, and Pearson correlation coefficients were calculated to examine associations between symptoms and emotional expressions. All data were cleaned and standardized prior to analysis. Results: Many users expressed anxiety (20.63%) and depression (19.59%), frequently associated with chemotherapy and sleep disturbances. Among reported symptoms, sleep problems carried the highest negative sentiment (79.81%), underscoring their profound impact on well-being. Topic modeling consistently revealed seven recurring themes, including treatment decision-making, symptom management, and concerns about family, demonstrating the layered and personalized experiences of older cancer patients and their caregivers. Conclusions This study explored treatment-related and symptom-related difficulties faced by older adults with cancer. Many reported significant emotional strain, especially anxiety, depression, and sleep disturbances. These findings highlight the necessity for supportive strategies addressing both psychological and physical aspects of care. Future research could investigate the utility of large language models in analyzing these narratives, provided the data is ethically managed and appropriate for such use.

BACKGROUND: PUVA therapy has been used to treat psoriasis, vitiligo, and various cutaneous diseases. Psoralens are metabolized in the liver and thus can produce liver damage. OBJECTIVE: This study was done to observe the effect of 5-methoxypsoralen on liver function during photochemotherapy. METHODS: Serum transaminase levels were determined before and during photochemotherapy in eighty patients with vitiligo or psoriasis. RESULTS: Four of the eighty patients who had received 5-MOP-PUVA showed elevation of serum transaminase levels. CONCLUSION: Although there have been few cases showing transient elevated values of serum transaminase during 5-MOP-PUVA, it is advisable to obtain liver function tests before and serially during photochemotherapy.
Ficusin ; Furocoumarins ; Humans ; Liver Function Tests ; Liver* ; Photochemotherapy* ; Psoriasis ; PUVA Therapy ; Transaminases* ; Vitiligo

OBJECTIVES: The aim of this study was to estimate the medical surge capacity required for mass prophylaxis based on a hypothetical outbreak of smallpox.METHODS: We performed a simulation using the Bioterrorism and Epidemic Outbreak Response Model and varied some important parameters, such as the number of core medical personnel and the number of dispensing clinics.RESULTS: Gaps were identified in the medical surge capacity of the Korean government, especially in the number of medical personnel who could respond to the need for mass prophylaxis against smallpox.CONCLUSIONS: The Korean government will need to train 1,000 or more medical personnel for such an event, and will need to prepare many more dispensing centers than are currently available.
Bioterrorism ; Korea ; Smallpox ; Surge Capacity ; Vaccination ; Variola virus

The number of patients is increasing and their mean age is also increasing. Proper dietary adjustments are necessary to prevent protein-calorie malnutrition or complications but it is difficult for dialysis patients to adapt to diet therapy due to stress or anorexia. Education does not consider the individual characteristics, knowledge, dietary inhabit education demands, and initial education. The purpose of this study was to identify dialysis patient's nutrition knowledge and, dietary practice and compare those with nutrition education or counseling demands for providing basic data of desirable nutrition management. The data were collected by a survey consisting of the general characteristics, disease related characteristics, nutrition education and counsel characteristics, level of nutrition knowledge, diet therapy, and nutrition education and counsel demands from the 28th March to 22th July 2017. The total number of subjects were 33 patients among dialysis patients at two tertiary medical institutions and an artificial kidney room at a private hospital in Incheon·Gyeonggi. The data collected were analyzed statistically using the SPSS program 23.0, followed by further analyses using frequency analysis, one-way ANOVA, cross analysis, and correlation analysis. The results of the dialysis patients showed that younger (P < 0.05), female (P < 0.05), abnormal high school diploma (P < 0.001) groups had high nutrition scores. In addition, dietary practice and nutrition education and counsel demands showed a positive correlation (P < 0.05, P < 0.01). In particular, females were higher than males in nutrition knowledge, dietary practice, nutrition education, and counseling demand scores.
Anorexia ; Counseling ; Dialysis ; Diet Therapy ; Diet ; Education ; Female ; Hospitals, Private ; Humans ; Kidney Failure, Chronic ; Kidneys, Artificial ; Male ; Protein-Energy Malnutrition

OBJECTIVES: The aim of this study was to estimate the medical surge capacity required for mass prophylaxis based on a hypothetical outbreak of smallpox. METHODS: We performed a simulation using the Bioterrorism and Epidemic Outbreak Response Model and varied some important parameters, such as the number of core medical personnel and the number of dispensing clinics. RESULTS: Gaps were identified in the medical surge capacity of the Korean government, especially in the number of medical personnel who could respond to the need for mass prophylaxis against smallpox. CONCLUSIONS The Korean government will need to train 1,000 or more medical personnel for such an event, and will need to prepare many more dispensing centers than are currently available.

Arterioureteral fistula is a rare but life-threatening condition. An endovascular treatment approach is commonly used; however, there is a potential risk of infection. We present a case in which a patient presented with abdominal pain and was diagnosed with a ruptured internal iliac artery aneurysm and a subsequent arterioureteral fistula. An aortic stent graft was inserted to treat the arterioureteral fistula, but it subsequently became infected, resulting in the patient's death. This case underscores the importance of early diagnosis and treatment of arterioureteral fistula and the possibility of graft infection.