1.Contents Analysis of Textbook Related to Safety Education in Elementary School.
Mikyung SONG ; Jungeun LEE ; Sunyoung MOON ; Sookja YANG ; Shinjeong KIM
Journal of Korean Academy of Community Health Nursing 2005;16(2):205-220
PURPOSE: The purpose of this study was to provide basic data to develop safety education programs in elementary school. METHOD: Analysis is made based on textbooks for 1st-6th-grade elementary school students published by Korea Ministry of Education & Human Resources Development. RESULTS: 1) Among the textbooks. only
Disasters
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Education*
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Emergencies
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Fires
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Humans
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Korea
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Play and Playthings
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Smoke
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Smoking
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Sports
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Staff Development
2.Web-Based Instruction For Public Health Officials-A Prototype Development And Preliminary Evaluation.
Jeehee KIM ; Eunkyeong JEONG ; Jongkoo LEE ; Sookja YANG ; Younghack SHIN ; Younghee YOON ; Byung Chul CHUN
Journal of Korean Society of Medical Informatics 2001;7(4):121-129
We developed a prototype of web-based instruction(WBI) program to provide more convenient and more effective service to the public health officials. This web-based instruction program had specific objectives on vaccination for the public health officials who were working for national immunization program. This course had 21 chapters composed of hypertext or voice-based instruction. Using the WBI courseware of Korean Officials Trainging Institution(KOTI), 32 public health official volunteers joined this prototype 2 week program in April 2001. A Auestionnaire survey for evaluation of subjective satisfaction and related factors, cyber-education behavior, and internet using behavior was performed in April, 2001. Among the answered 29 subjects, 24 were female(82.8%). Mean age was 36.6% 6.16. 79.3% of the 29 subjects connected internet in their office. The highest frequent study duration was < 1 hr(51.7%) per connection. They represented relatively high subjective satisfaction in education form(78.6%), and contents(85.7%). But 62.3% of these subjects answered the subjective relative effectiveness score(which was as 100 in case of classical lecture form) was lower than 100. The place to connect internet was only statistically significant factor between these two groups in bivariable analysis. But we could not found any significant factors in multivariate analysis. 89.7% of the subjects answered they would like to participate any more WBI courses serviced by Korean National Health Institute. This study had restriction of small sample size.
Education
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Education, Distance
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Hypermedia
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Immunization Programs
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Internet
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Multivariate Analysis
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Public Health*
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Sample Size
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Vaccination
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Volunteers
3.Web-Based Instruction For Public Health Officials-A Prototype Development And Preliminary Evaluation.
Eunkyeong JEONG ; Jeehee KIM ; Sookja YANG ; Younghack SHIN ; Younghee YOON ; Byung Chul CHUN
Journal of Korean Society of Medical Informatics 2001;7(4):109-119
Health and Welfare Training Center of Korean National Institute of Health (KNIH) has developed various education curricula for the officials who involved in the public health or welfare sectors. Still almost all education programs are off-line based. In off-line settings, both the lecturers and the students should come to KNIH from their counties to join the education programs, and it is impossible for the students to review or to re-practice the education contents. From September to October, 2000, we conducted a basic survey to evaluate the information infrastructure, internet use and attitude to the cyber education. The 183 institutions and 548 public health officials answered our questionnaire. The informationa infrastructure of each institution was not satisfactory for distance education via world wide web. The proportion of e-mail user was only about 62% of the answered subjects. But cyber- education was highly needed in both institutions and the surveyed officials. They answered that they would actively participate in the cyber-education(97%). The officials expected that the online education would be effective to acquire information, to increase the chance for participation and to reduce the education cost. The expected barriers of the cyber-education were the overload when they should undergo simultaneously education and working, lack of proper infrastructure, disinterest of their low capability of internet use.
Curriculum
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Education
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Education, Distance
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Electronic Mail
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Humans
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Internet
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Public Health*
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Surveys and Questionnaires
4.Transgenic mice with overexpression of human scavenger receptor A on endothelial cells
LX WAN ; YZ YANG ; MJ WU ; ZY WAN ; Kim Sookja CHUNG ; S.M Stephen CHUNG ; Marcella MA ; DL CAO ; X CHEN
Chinese Medical Journal 2001;114(10):1078-1083
Objectives To establish a new transgenic mouse model for determining the function and role of human scavenger receptor A (SR-A) in atherosclerosis in vivo. Methods Human scavenger receptor minigene-driven mouse tie-1 promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via the microinjection method. PCR and Southern blot were used to screen the positive transgenic mice. RT-PCR and immunohistochemical analysis were used to detect the level and location of human SR-A Ⅰ expression in transgenic mice. The activity of human SR-A Ⅰ was determined by morphologic observation of aortic endothelial cells of transgenic mice under transmission electron microscopy. Results The electrophoresis assay showed the expected 4 fragments of 0.9 kb, 1.1 kb, 1.2 kb and 4.2 kb in the Sma Ⅰ digest and 2 fragments of 0.8 kb and 6.7 kb in Bgl Ⅱ digest of plasmids pTie-1/hSR-A. The fragment sequence of tie-1 promoter and human SR-A cDNA in plasmids pTie-1/hSR-A was correct and no ATG before the translation initiation sites of human SR-A was found by sequence analysis. 561 injected and surviving embryos with the purified human SR-A minigene were implanted into the oviducts of 19 ICR pseudopregnant mice. Among the 54 surviving pups from 13 foster mothers, 7 were identified by PCR and Southern blot analysis. The results of RT-PCR and imrnunohistochemical analysis showed human SR-A was specifically expressed on vascular endothelial cells of the aorta and renal artery, as well as hepatic sinusoidal endothelial cells in transgenic mice. Transmmion electron microscope (TEM) of aorta of transgenic mice showed that a large number of vesicles, multivesicle bodies and swollen mitochondria filled the plasma of endothelial cells. Conclusions A transgenic mouse model with overexpression of human SR-A in endothelial cells was successfully established. The transgene was integrated and transmitted into the chromosome of transgenic mice. Tie-1 promoter controlled the transgene to express in endothelial cells in mice. Pinocytic activity of aortic endothelial cells in transgenic mice was higher than that of C57BL/6J mice. Our studies will provide a new transcgenic model for investigation of atherosclerosis and functions of human SR-A.