35 samples of Korean fermented foodstuffs were tested to isolate and to identify for aflatoxins. Aflatoxin G1 was detected in samples of soybean and Kanjang (Soybean sauce), are aflatoxins G1 & G2 in Meju (fermented soybean mass) are Dwenjang (fermented soybean paste). In the culture media of Aspergillus flavus aflatoxins B1, B2, G1 and G2 were also isolate and identified. Aflatoxins were confirmed by the thin layer chromatography with methancl : chroroform (5:95v/v) developer and the ultra violet absorption spectrum.
Absorption ; Aflatoxins* ; Aspergillus flavus ; Chromatography, Thin Layer ; Culture Media ; Soybeans ; Viola

This study was carried out to investigate air pollution by total suspended particulate(T.S.P.), benzene soluble matter and benzo(a)pyrene in Seoul city. The sampling areas were divided into commercial(Kwang Hwa Moon), industrial(Ku Ro Dong) and residential area(Shin Chon). Sampling was undertaken by High Volume Air Sampler for four seasons from January 1977 to November 1977. The T.S.P. was extracted with Soxhlet apparatus by benzene and benzo(a)pyrene was separated by column chromatography and thin layer chromatography. The concentrations of benzo(a)pyrene were measured by means of fluorophotometer, and following results were obtained. 1. Arithmetic average concentration for 1-day averaging time of total suspended particulate were 275.6 microgram/m3 in Kwang Hwa moon, 325.9 microgram/m3 in Ku Ro Dong and 193.0 microgram/m3 in Shin Chon. 2. The seasonal variance of total suspended particulate at Ku Ro Dong and Shin Chon were 102.7 microgram/m3 99.6 microgram/m3 respectively and at Kwang Hwa Moon 39.9 microgram/m3. And the concentration of autumn is higher than of that spring at Ku Ro Dong and at Shin Chon, but at Kwang Hwa Moon, the seasonal variance is very little. 3. The concentrations of 50% frequency from geometric mean for 1-day averaging time were 264 microgram/m3 and 178 microgram/m3 at Kwang Hwa Moon, Ku Ro Dong and Shin Chon. And geometric standard deviation were 1.27, 1.38 and 1.41 respectively. 4. The concentrations of benzene soluble mater were 26.9 microgram/m3 Kwang Hwa Moon, 22.7 microgram/m3 at Ku Ro Dong and 15.5 microgram/m3 at Shin Chon, and the ratios to the T.S.P. were 9.8%(range 5.6-14.8%), 7.0%(range 2.4-14.4%) and 8.0%(range 5.5-22.1%) respectively. 5. The concentrations of benzo(a)pyrene were 8.5 microgram/m3 (range 0.8-29.9 microgram/m3 ) at Kwang Hwa Moon 10.9 microgram/m3 (range 1.1-52.0 microgram/m3 ) at Ku Ro Dong and 5.8 microgram/m3 (range 1.5-11.4 microgram/m3) at Shin Chon. 6. The results of this investigation were relatively high in compared with the recommended standards of suspended particulate in air of U.S. Environmental Protection Agency and observed levels of Benzo(a)-pyrene in U.S. city.
Air Pollution ; Atmosphere* ; Benzene ; Benzo(a)pyrene* ; Chromatography ; Chromatography, Thin Layer ; Seasons ; Seoul* ; United States Environmental Protection Agency

This study was attempted to know that the interactions of various fungi, and methionine and MgSO4 introduced as the substrate of culture media for fungi were affected to produce aflatoxins by Asp. flavus. 5 different fungi were isolated from the fermented soybean mash and were cultured in Chemically Defined medium (C.D. media) and soybean mash at 25 degrees for 10 days. (1) It was confirmed that Asp. flavus produced aflatoxins in the C.D. medium and soybean mash, but that Asp. niger, Asp. oryzae, Asp. awamori and Asp. terreus did not produced them respectively. (2) Asp. flavus cultured with Asp. niger did not produce aflatoxins in C.D. medium, but produced in soybean mash, in other hand, Asp. flavus with other fungi except Asp. niger produced aflatoxins in C.D. medium and soybean mash. (3) The growth of fungi were more prosperous in the separate culture than in the mixed culture. (4) In the C.D. medium added 20% of cultured medium of Asp. niger, Asp. flavus did not produce aflatoxins but other cultured medium did not prohibit the production of aflatoxins by Asp. flavus. (5) On the contrary, MgSO4 increasing the productivity of aflatoxins by Asp. flavus in the C.D. medium methionine known as one of precurser of aflatoxins did not affected the increasing productivity with significance.
Aflatoxins* ; Culture Media ; Efficiency* ; Fungi* ; Hand ; Methionine ; Niger ; Oryza ; Soybeans ; Viperidae*

In order to investigate air pollution by heavy metals in Seoul city, the suspended particulates in the atmosphere were sampled with high volume air sampler in industrial area(Ku Ro Dong), commercial(Kwang Hwa Moon) and residential(Shin Chon Dong) from January to November, 1977. The sampled suspended particulates were digested and extracted from suspended particulates with the acidic solution by reflux-extraction technique, and were measured by atomic absorption spectrophotometry. And Mercury was measured by mercury analyzer applying the reducing sublimation technique. Among heavy metals analyzed, the iron was identified at th highest level in the suspended particulates and the chromium was the least. Through the surveyed area, the concentration of heavy metals of the industrial area was comparatively high among others and the commercial was the second. It was detected that lead was the most concentrated in the suspended particulate of the commercial area, that might be caused of the traffic emissions. The seasonal variations were analyzed and the correlations among heavy metals and total suspended particulate were also calculated. Especially, the iron was highly correlated with total suspended particulate in all the surveyed areas.
Air Pollution ; Atmosphere* ; Chromium ; Iron ; Metals, Heavy* ; Seasons ; Seoul* ; Spectrophotometry, Atomic ; Sublimation

The particle size of suspended particulates was measured by a Andersen air sampler from Mar. 1982 to Feb. 1984 in a part of Seoul. It was concluded as follows: 1) The arithmetic concentration of suspended particulates was 147.8 microgram/m3 in Spring, 136.9 in Summer, 131.9 in Autumn and 158.1 in Winter respectively. 2) The cumulative distribution of suspended particulates size in logarithmic diagram showed similar to normal log distribution. 3) The atmospheric particulate matters showed a bimodal size distribution on the base of unit particle concentrations, which divided at approximately 2 micrometer in the diameter. 4) While the fine particulates less than 2.1 micrometer was 35.4-45.0%, the coarse particulates was 55.0-64.5%. 5) The higher the concentration of suspended particulates, the more increased the ratio of fine particulates. The higher the concentration of suspended particulates, the lower median size of suspended particulate as well. 6) The respirable dust particulates less than 4.7 micrometer was 52.2-62.9% in seasonal average through the 2 year samples. With the above result, air pollution concerned with public health could be evaluated and the control measures also are suggested.
Air Pollution ; Atmosphere* ; Dust ; Particle Size* ; Public Health ; Seasons ; Seoul*

The pulmonary microvasculatures of rats were studied by injection replicas prepared from Mercox. This medium enabled us to easily obtain consistent, stable, and complete injected replicas of the pulmonary vasculature. In order to investigate the three dimensional structures of the tributaries of the bronchial artery, such as the capillary plexus and vasa vasorum, we performed a scanning electron microscopic(SEM) study of the vascular casts, using Mercox CL-2B as a media. The alveolar capillaries revealed hexagonal or pentagonal rings of vascular networks. In some areas, the vascular rings composed a square network, The bronchial tree was supplied by the bronchial arteries which form a coarse capillary extending as far as the terminal bronchioles. Occasionally the capillary plexus was connected with adjacent capillary networks in and around the alveolar walls. The walls of the pulmonary artery revealed only a single layer of the vasa vasorum, but those of the pulmonary vein were surrounded by more complicated and well developed vasa vasorum than the pulmonary arterial side. The mean diameters of the venous vasa vasorum are greater than the arterial vasa vasorum.
Rats ; Animals

This study was carried out to investigate the morphologic characteristics and localization of antigenic molecules of Pneumocystis carinii in experimentally induced P. carinii pneumonia in rats. After six weeks of administration of low protein diet and dexamethasone, Sprague-Dawley rats were sacrificed to submit lungs or bronchoalveolar lavage for the study. Monoclonal (092, 900, 902, and 904) and polyclonal (SP-D) antibodies were used for immunohistochemistry and immunoelectron microscopy (ITEM and ISEM). Immunohistochemically P. carinii organisms were well identified as clusters or separated forms in the alveolar spaces being frequently attached to the alveolar walls. Immunoelectron microscopically the adherences of gold particles were observed on the surface of all stages of the P. carinii. Occasionally positive immunogold labeling was observed in the cytoplasm of the trophozoites and on the pellicle of the intracystic bodies within the cysts. The monoclonal antibodies 092, 900, 902, and 904 reacted mainly with pellicles of P. carinii, whereas SP-D labeled on the pellicles, intracystic bodies, cytoplasms of the alveolar macrophages, and free floated surfactant material in the alveolar spaces. The immunogold particles were observed more diffusely and intensely in the cysts than in the trophozoites. These results indicate that antigen is mainly localized on the pellicles, and accumulated during development from the trophozoite to the cyst stages.
Animals ; Antibodies ; Antibodies, Monoclonal ; Bronchoalveolar Lavage ; Cytoplasm ; Dexamethasone ; Diet, Protein-Restricted ; Immunohistochemistry ; Lung ; Macrophages, Alveolar ; Microscopy, Electron ; Microscopy, Immunoelectron ; Pneumocystis carinii* ; Pneumocystis* ; Pneumonia ; Pulmonary Surfactant-Associated Protein D ; Rats ; Rats, Sprague-Dawley ; Trophozoites

No abstract available.
Methemoglobinemia*

This study was carried out to investigate the mechanisms of acinar cell deletion, leading to the pancreatic atrophy of rat pancreas after experimental duct ligation. Fifty-seven male Sprague-Dawley rats, maintained on a stock diet, weighing 200 gm, in average, were divided into 2 experimental groups. Group 1. Control group. Six rats. Abdominal cavity was opened and closed without further treatment. Group 2. Fifty-one rats. Animals were treated with partial ligation of the pancratic ducts according to the procedure developed by Hultquist followed by sequential sacrifices at: 1 hour (3 rats), 3 hours (3 rats), 6 hours (6 rast), 12 hours (3 rats) and 24 hours (8 rats); 2 days (8 rats), 3 days (3 rats), 4 days (3 rats) and 5 days (5 rats); 1 week (3 rats), 2 weeks (3 rats) and 8 weeks (3 rats); after partial ligation was extirpated and examined by both light and electron microscopy. The results obtained were as follows: Light microscopically, noted were an interstitial edema and focal necrosis of the pancreatic tissue along with fine vacuolization and depletion of the zymogen granules in the acinar cell cytoplasms and condensation of the acinar cell nucleus. These changes were observed by 2 days after ligation. At about the same time, one can observe the dense body, identified to be apoptotic body, in the acinar cell which were found to be decreased in quantity. By 5 days after ligation, no recognizable acinar cells left in the collagenous stroma except intercalated ducts. Conspicuous stroma except intercalated ducts. Conspicuous stromal hyalinization, thereafter. Electron microscopically (TEM and SEM), nuclear condensation and margination toward the nuclear membrane was noted by 6 hours after duct ligation. By 24 hors sporadic membrane-bounded apoptotic bodies appeared in the acinar cells, the number of which reaching to the peak by 3 days after ligation. These apoptotic bodies were found to be phagocytosed by either intraepithelial mononuclear phagocytes or adjoining acinar cells. It can be concluded, therefore: That orderly remodeling of pancreatic exocrine tissue during atrophy is effected by rapid deletion of acinar cells by apoptosis.
Male ; Humans ; Rats ; Animals

Lactic dehydrogenase (LDH) isozyme pattens were examined in rats after exposing the animal to 250 ppm of sulfur dioxide gas. The isozymes of the respective tissues were separated on cellulose-acetate strips from the brain, lung, heart, liver, kidneys, and muscle, and visualized as the isozyme bands by the formazan reaction and analyzed by densitometry. As well as the above experiment, room-air and room-air+SO2 were aerated through tissue homoenates in-vitro, accompanied by pure oxygen aeration in order to see the in vitro effect of the gases on the LDH activity in the tissues mentioned with the following conclusions. (1) The H-type of LDH activity dominated in the normal heart tissue of rats, M-type in the normal lung, liver, and muscle tissues of the animal. (2) The kidney tissue of normal rats exhibited preponderance of LDH-1 and-5 isozymes, brain tissue in LDH-1 and-4 isozymes. (3) When rats inhaled sulfur dioxide gas in the concentration of 250 ppm, it appeared that the M-type tended to predominate in the anaerobic tissues and the H-type in the aerobic tissue. (4) The degree of oxygen tension seemed to be correlated with the low level of LDH activity in the anaerobic tissues such as liver and muscle and with the increased activity in the aerobic tissues, such as heart and lung. (5) The low oxygen tension seems to favor syn-thesis of M-type LDH and high oxygen tesnion H-type LDH in the tissues of rats.
Air Pollution ; Animal ; Citric Acid Cycle ; Densitometry ; Electrophoresis ; Environmental Exposure ; Isoenzymes ; Lactate Dehydrogenase/metabolism* ; Oxygen/metabolism ; Rats ; Sulfur Dioxide/toxicity* ; Substances: ; Isoenzymes ; Sulfur Dioxide ; Oxygen ; Lactate Dehydrogenase