Rabbits were immunized with free-living amoebas by intravenous injections. The amoebas were Acanthamoeba culbertsoni and Naegleria fowleri and obtained by axenic cultivation in CGVS medium. Each rabbit received 10(6) of Acanthamoeba culbertsoni and 10(5) of Naegleria fowleri trophozoites respectively every other day in three doses and finally one booster dose at 1 week later. Antiserum was collected from thc following day of the booster injection up to 2 months period, and stored at -30 degree C until use. The immobilization test was performed. One drop of amoeba suspension was mixed with the test serum on slide and observed the mobile state under microscope. Maximal immobilizing phenomenon observed in 30 minutes and, then gradually recovered to normal state. Inactivation of antiserum at 56 degree C for 30 minutes did not affect the immobilization phenomenon. The immobilization rates decreased by the serial dilution of antiserum. At dilution more than 1:8, the immobilization was almost the same as in the normal serum. The immobilizing antibody in anti-Acanthamoeba culbertsoni rabbit serum showed highest titre in 3rd day after booster immunization and from first to 6th week in anti-Naegleria fowleri rabbit serum. Cross matching of Acanthamoeba culbertsoni and Naegleria fowleri showed antigenic difference of the two species. It is suggested that the immobilization reaction may be of value as a supplementary test in the diagnosis of primary amoebic meningoencephalitis.
parasitology-protozoa ; free-living amoeba ; Naegleria fowleri ; Acanthamoeba culbertsoni ; rabbit ; immunology

A case of Down's syndrome in which transient myeloproliferative disorder developed is described. In hematologic findings of peripheral blood, high blast cell count on 1st day of birth had been found and after serial follow-up for several weeks, decrease in WBC counts from 22.6x10(9)/L to 7.5x10(9)/L and blast cell counts from 31% to 2% occurred. The karyotype of his patient was 45,XY, der (13;14) (q10;q10), der(14;21) (q10;q10), +21. Karyotyping of his father revealed 45,XY, der(13;14) (q10;q10). Without specific chemotherapy, hematologic and clinical recovery was occurred within several weeks. We deport a case of transient myeloproliferative disorder in Robertsonian translocatlon type of Down's syndrome accompanying another Robertsonian translocation, der (13;14) (q10;q10), inherited from his father.
Cell Count ; Down Syndrome ; Drug Therapy ; Fathers ; Follow-Up Studies ; Humans ; Karyotype ; Karyotyping ; Myeloproliferative Disorders* ; Parturition

No abstract available.
Hepatitis A Antibodies* ; Hepatitis* ; Immunoglobulin M*

No abstract available.
Antibodies* ; Blood Donors* ; Chemistry* ; Chlamydia* ; Chlamydophila pneumoniae* ; Humans ; Mycoplasma pneumoniae* ; Mycoplasma* ; Pneumonia, Mycoplasma* ; Prevalence*

PURPOSE: The purpose of this study was to provide nursing intervention with basic data extracted through investigating self-care and nursing of lymphedema in patient who have had a mastectomy. METHOD: The subjects for this study consisted of 214 mastectomy patients in 2 hospitals. The data were collected from December 1, 2004 to February 28, 2005. The instruments used for this study were both the Self-care Practice Scale by Cho, Myoung-Ok et al.(2003) which we modified and the Nursing Evaluation Scale developed by the researchers. RESULTS: The mean score of self-care on lymphedema was 59.96+/-12.46. The mean score of nursing was 25.64+/-9.25. Factors influencing the self-care were the postoperative period (F= 17.505, p<.001), the location of the tumor (F= 3.826, p=.023), menstruation (t=7.333, p=.007), and complications (F=5.427, p<.001). CONCLUSION: Self-care and nursing care on the lymphedema management were shown to be lower than expected. Especially, the score of self-care was the lowest in the more than 3 year postoperative period. Therefore, the longer the postoperative period is, the more active management on lymphedema needs to be. Also, education should be given to both nurses and patients on the importance of self-care on lymphedema with mastectomies.
Education ; Female ; Humans ; Lymphedema* ; Mastectomy* ; Menstruation ; Nursing Care* ; Nursing* ; Postoperative Period ; Self Care*

No abstract available.
Child* ; Humans ; Infant*

No abstract available.
Beckwith-Wiedemann Syndrome*

PURPOSE: We evaluated a rapid preparation procedures for the labeling and quality control of 99mTc-ECD, MAG3, and MIBI using microwave heating and Sep-Pak cartridges. MATERIALS AND METHODS: 99mTc labeling of ECD, MAG3, and MIBI kit preparation was performed according to the package inserts with microwave heating modification. Heating time was 10-15 sec, and heating was performed with 3 mm plastic bottle with screw cap to prevent radiation contamination. Labeling efficiency was obtained with C18 or Alumina N Sep-Pak cartridges. RESULTS: The radiochemical purity of 93~96% for 99mTc-ECD and 95~99% for 99mTc-MIBI was obtained using Alumina N Sep-Pak cartridge. The optimum irradiation time of microwave method for 3 ml 99mTc-labeled radiopharmaceutical solution was 10 sec for 99mTc-ECD and 99mTc-MIBI, and 15 sec for 99mTc-MAG3. The RESULTS of quality control data with Sep-Pak cartridges were well correlated with TLC method. The total preparation time of these radiopharmcaeuticals was 5~6 min including quality control procedure. CONCLUSION: This study demonstrates that radiopharmaceuticals preparation by microwave heating and quality control by Sep-Pak cartridges can be efficiently utilized as an alternative to the recommended method by manufacturer's manual.
Aluminum Oxide ; Heating* ; Hot Temperature* ; Microwaves* ; Plastics ; Product Labeling ; Quality Control* ; Radiopharmaceuticals ; Technetium Tc 99m Mertiatide

No abstract available.
Sepsis* ; Serratia marcescens* ; Serratia*

PURPOSE: This study was conducted to identify the personality type, problematic drinking pattern, and stress coping style of the nasal bone fracture patients by comparing those with general fracture patients. METHODS: 50 nasal bone fracture patients due to violence and 50 general fracture patients due to simple trauma, such as fall and traffic accident were conveniently selected. RESULTS: The study results showed that nasal bone fracture patients due to violence significantly presented higher level of A type personality and more problematic drinking pattern than general fracture patients due to simple trauma. Stress coping style, however, did not show significant difference between two groups. CONCLUSION: In order to prepare prevention strategies for basal bone fracture in the future, health education alter problematic drinking habit to sound drinking pattern may be offered and provided nursing interventions tailored to A type personality.
Accidents, Traffic ; Adaptation, Psychological ; Drinking ; Fractures, Bone ; Health Education ; Humans ; Nasal Bone ; Type A Personality ; Violence