No abstract available.
Dermatitis* ; Streptococcus pyogenes

PURPOSE: To evaluate pathologic changes of the trachea and the lung after insertion of silicone covered and non-covered Gianturco stent in the trachea of dog. MATERIALS AND METHODS: Silicone covered(covered) and non-covered(bare) Gianturco stents, six in each, were inserted into the tracheal lumen of six consecutive dogs. After 1--10 week observation, the dogs were sacrificed and their tracheas and lungs were examined grossly and histopathologically. Serial chest radiographs were performed to evaluate pneumonia and stent migration every 3--5 days. RESULTS: Pneumonia was observed in one of bare stent group and five of covered stent group. Stent migration was noted in three of covered stent group. In bare stent group, the epithelium of the trachea was markedly thickened by hyperplasia and stent was covered by granulation tissue from 6 weeks after stent insertion. Inflammation was focal at contact site between the stent and the trachea. In covered stent group, the epithelium was denuded at multiple areas and there were multifocal squamous metaplasia of the mucosa. Inflammatory reaction of the trachea was diffuse. CONCLUSION: Inflammatory reaction was more severe and stent migration was more frequent in covered stent group. The use of covered stent in the trachea should be studied further.
Animals ; Dogs ; Epithelium ; Granulation Tissue ; Hyperplasia ; Inflammation ; Lung ; Metaplasia ; Mucous Membrane ; Pneumonia ; Radiography, Thoracic ; Silicones* ; Stents* ; Trachea

PURPOSE: The purpose of this study was to evaluate the effectiveness of percutaneous fine-needle aspiration (PCNA) in malignant and benign mediastinal lesions. MATERIALS AND METHODS: PCNA of mediastinal lesions were performed in 46 patients using 22 Guage thin-walled Westcott type needle under fluoroscopy guidance. There were 24 malignant mediastinal masses, 18 of which were diagnosed only by PCNA and six of which were proven surgically. Of 22 benign mediastinal masses, 19 cases were proven surgically. RESULTS: In 24 patients with malignant neoplasms, a positive diagnosis of malignancy was established in 18 (75%). One suspicious malignancy and five false negative results occurred. In 22 patients with benign masses, a specific diagnosis was established in six (27.3%), and 13 patients revealed nonspecific benignanay (59.1%). Three benign masses were reported as unsatisfactory. Five patients developed pneumothorax as a complication, and one of these needed a chest tube. CONCLUSION: Percutaneous fine-needle aspiration in mediastinal masses can differentiate benign mass from malignancy and can make a pathologic diagnosis.
Biopsy, Fine-Needle* ; Chest Tubes ; Diagnosis ; Fluoroscopy ; Humans ; Needles ; Pneumothorax ; Proliferating Cell Nuclear Antigen

PURPOSE: The purpose of this study was to evaluate the effectiveness of percutaneous fine-needle aspiration (PCNA) in malignant and benign mediastinal lesions. MATERIALS AND METHODS: PCNA of mediastinal lesions were performed in 46 patients using 22 Guage thin-walled Westcott type needle under fluoroscopy guidance. There were 24 malignant mediastinal masses, 18 of which were diagnosed only by PCNA and six of which were proven surgically. Of 22 benign mediastinal masses, 19 cases were proven surgically. RESULTS: In 24 patients with malignant neoplasms, a positive diagnosis of malignancy was established in 18 (75%). One suspicious malignancy and five false negative results occurred. In 22 patients with benign masses, a specific diagnosis was established in six (27.3%), and 13 patients revealed nonspecific benignanay (59.1%). Three benign masses were reported as unsatisfactory. Five patients developed pneumothorax as a complication, and one of these needed a chest tube. CONCLUSION: Percutaneous fine-needle aspiration in mediastinal masses can differentiate benign mass from malignancy and can make a pathologic diagnosis.
Biopsy, Fine-Needle* ; Chest Tubes ; Diagnosis ; Fluoroscopy ; Humans ; Needles ; Pneumothorax ; Proliferating Cell Nuclear Antigen

OBJECTIVES: Despite severe oligospermia, males with Y chromosome microdeletion can achieve conception through ICSI (Intracytoplasmic Sperm Injection). However, ICSI may not only result in the transmission of microdeletions but also the expansion of deletion to the offspring. The purpose of this study was to screen vertical transmission, expansion of microdeletions and de novo deletion in male fetuses conceived by ICSI. MATERIALS AND METHODS: A total of 32 ICSI treated patients with their 33 (a case of twin) male fetuses conceived by ICSI were used to make this study group. Sequence-tagged sites (STSs)-based PCR analyses were performed on genomic DNA isolated from peripheral blood of fathers and from the amniocytes of male fetuses. Ten primer pairs namely, sY134, sY138, MK5, sY152, sY147, sY254, sY255, SPGY1, sY269 and sY158 were used. The samples with deletions were verified at least three times. RESULTS: We detected a frequency of 12.5% (4 of the 32 patients) of microdeletions in ICSI patients. In 4 patients with detected deletions, two patients have proven deletions on single STS marker and their male fetuses have the identical deletion in this region. Another two patients have two and three deletions, but their male fetuses have more than 3 deletions which include deletions to their father's. Meanwhile, seven male fetuses, whose fathers were analyzed to have all 10 STS markers present, have deletions present in at least one or more of the markers. CONCLUSIONS: Although the majority of deletions on the Y chromosome are believed to arise de novo, in some cases a deletion has been transmitted from the fertile father to the infertile patient. In other cases the deletion was transmitted through ICSI treatment, it is likely that one sperm cell is injected through the oocyte's cytoplasm and fertilization can be obtained from spermatozoa. Our tests for deletion were determined by PCR and our results show that the ICSI treatment may lead to vertical transmission, expansion and de novo Y chromosome microdeletions in male fetuses. Because the sample group was relatively small, one should be cautious in analyzing these data. However, it is important to counsel infertile couples contemplating ICSI if the male carries Y chromosomal microdeletions.
Cytoplasm ; DNA ; Family Characteristics ; Fathers ; Fertilization ; Fetus* ; Humans ; Male* ; Oligospermia ; Polymerase Chain Reaction ; Sequence Tagged Sites ; Sperm Injections, Intracytoplasmic* ; Spermatozoa ; Y Chromosome*

Gynecomastia is a proliferation of the glandular component of the male breast. Gynecomastia is the most common variant condition of the male breast and results from the effect of an altered estrogen-androgen balance on breast tissue or from the increased sensitivity of breast tissue to a normal estrogen level. We experienced a case of macrogynecomastia in a 16-year-old boy who had a normal male phenotype and was treated by reduction mammoplasty with breast tissue removal, which was effective and produced good cosmetic and psychologic results.
Adolescent ; Breast ; Estrogens ; Female ; Gynecomastia ; Humans ; Male ; Mammaplasty* ; Phenotype

OBJECTIVES: To access the effectiveness of radiofrequency myolysis (RFM) in women with midline dysmenorrhea. METHODS: We designed RFM in two ways laparoscopic RFM (LRFM), vaginal ultrasound-guided RFM (URFM). One hundred and thirty-two patients were in the LRFM group and, 140 patients were in the URFM group. RESULTS: Upon receipt of surgery, both the LRFM and the URFM groups demonstrated a significant decrease (P < 0.001) in the mean pain score when compared to those before and after surgery. CONCLUSION: The RF uterine myolysis procedure provides an alternative for those patients who suffer from intractable midline dysmenorrhea. LRFM is an alternative choice because it is relatively safe and, simple to perform and moreover, it is satisfactory. LRFM appears to increasingly succeed in the treatment of midline dysmenorrhea.
Dysmenorrhea* ; Female ; Humans ; Laparoscopy ; Leiomyoma ; Ultrasonography

TO know the causative agent of asptic meningitis. we performed and cerebrospinal fluid study on 24 children who had been admitted to the department of Pediatrics.. Asam Medical Center form Jume. 1991 to July 1991. In the 10 cases of them. CSF virus culture was done Special Reference Laboratory, Japan The most of the patients were young infants, and 15 cases of them were under 3 months of age. The male to female ratio was 1:1.4 We observed the main symptoms as fever 100%. irritability 67%, vomiting 17%. poor feeding 13%, seizure 8%. In the 4 cases among the 10 cases with virus culture, Coxsackievirus B5 was cultured. We concluded that the causative agent of this epidemic aseptic meningitis in 1991 was Coxsakievirus B5.
Cerebrospinal Fluid ; Child ; Female ; Fever ; Humans ; Infant ; Japan ; Male ; Meningitis ; Meningitis, Aseptic* ; Pediatrics ; Seizures ; Vomiting

Purpose: Alzheimer disease (AD) is one of the most complex diseases and is characterized by AD-related neuropathological features, including accumulation of amyloid-β plaques and tau neurofibrillary tangles. Dysregulation of alternative splicing (AS) contributes to these features, and there is heterogeneity in features across brain regions between AD patients, leading to different severity and progression rates; however, brain region-specific AS mechanisms still remain unclear. Therefore, we aimed to systemically investigate AS in multiple brain regions of AD patients and how they affect clinical features. Methods: We analyzed RNA sequencing (RNA-Seq) data obtained from brain regions (frontal and temporal) of AD patients. Reads were mapped to the hg19 reference genome using the STAR aligner, and exon skipping (ES) rates were estimated as percent spliced in (PSI) by rMATs. We focused on AD-risk genes discovered by genome-wide association studies, and accordingly evaluated associations between PSI of skipped exons in AD-risk genes and Braak stage and plaque density mean (PM) for each brain region. We also integrated whole-genome sequencing data of the ascertained samples with RNA-Seq data to identify genetic regulators of feature-associated ES. Results: We identified 26 and 41 ES associated with Braak stage in frontal and temporal regions, respectively, and 10 and 50 ES associated with PM. Among those, 10 were frontal-specific (CLU and NTRK2), 65 temporal-specific (HIF1A and TRPC4AP), and 26 shared ES (APP) that accompanied functional Gene Ontology terms, including axonogenesis in shared-ES genes. We further identified genetic regulators that account for 44 ES (44% of the total). Finally, we present as a case study the systematic regulation of an ES in APP, which is important in AD pathogenesis. Conclusions This study provides new insights into brain region-dependent AS regulation of the architecture of AD-risk genes that contributes to AD pathologies, ultimately allowing identification of a treatment target and region-specific biomarkers for AD.

True malignant mixed tumor (carcinosarcoma) of the salivary gland is an extremely rare tumor. By definition, it is a heterologous neoplasm that's composed of both malignant epithelial and mesenchymal components. We report here on a case of an 83 year old female patient with a parotid gland mass. Histologic examination showed a biphasic pattern of growth with both a poorly differentiated epithelial component and a sarcomatous spindle cell background. The immunohistochemical and ultrastructural findings support the diagnosis of a small cell neuroendocrine epithelial tumor and an undifferentiated malignant mesenchymal features. The histologic and ultrastructural features along with the immunohistochemical findings are presented. We also review the literature and we discuss the different opinions on the exact histogenesis of the true malignant mixed tumor of the salivary gland. To the best of our knowledge, this histological pattern has not been previously reported in the English medical literature.
Female ; Humans