1.Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis.
The Korean Journal of Parasitology 2004;42(3):145-148
We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
Animals
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Base Sequence
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China
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Clonorchis sinensis/enzymology/*genetics
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Comparative Study
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DNA, Helminth/chemistry/*genetics
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DNA, Mitochondrial/chemistry/*genetics
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DNA, Ribosomal/chemistry/genetics
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DNA, Ribosomal Spacer/chemistry
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Electron Transport Complex IV/genetics
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Genetic Markers
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Korea
;
Molecular Sequence Data
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RNA, Ribosomal, 18S/genetics
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Research Support, Non-U.S. Gov't
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Sequence Alignment
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Species Specificity
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*Variation (Genetics)
2.Molecular phylogenic location of the Plagiorchis muris (Digenea, Plagiorchiidae) based on sequences of partial 28S D1 rDNA and mitochondrial cytochrome C oxidase subunit I.
Soo Ung LEE ; Sun HUH ; Woon Mok SOHN
The Korean Journal of Parasitology 2004;42(2):71-75
To determine the molecular phylogenic location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenic relationship between members of the Plagiorchiidae, as suggested by morphologic features.
Animals
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Base Sequence
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DNA, Helminth/chemistry/genetics
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DNA, Mitochondrial/chemistry/genetics
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Electron Transport Complex IV/chemistry/*genetics
;
Molecular Sequence Data
;
Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 28S/chemistry/*genetics
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Sequence Alignment
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Support, Non-U.S. Gov't
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Trematoda/classification/*genetics
3.A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea.
Hyun Young PARK ; Soo Ung LEE ; Sun HUH ; Yoon KONG ; Jean Francois MAGNAVAL
The Korean Journal of Parasitology 2002;40(3):113-117
We investigated the sero-prevalence of toxocariasis among healthy Korean adults in 1999. A total of 314 sera from normal inhabitants in Whachon-gun, Gangwondo, Korea was examined for specific antibody levels against excretory-secretory products of second stage larvae of Toxocara (TES). The presence of cross-reactions with other helminthiases such as cysticercosis, paragonimiasis, sparganosis or clonorchiasis was also checked by specific IgG ELISA. Sera showing positive reaction against TES were also tested by IgG immunoblot and by IgE ELISA. Out of 314 subjects, 16 was found to be positive by TES IgG ELISA and immunoblot, among whom 12 were also positive by TES IgE ELISA. Among the 16 seropositive samples, two sera showed positive reaction against Paragonimus and sparganum antigen, respectively. These results inferred that cross-reactions were negligible between toxocariasis and other helminthiases. Toxocariasis seroprevalence among Korean rural adults was detected to be approximately 5%.
Adolescent
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Aged
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Aged, 80 and over
;
Animals
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Antibodies, Helminth/blood
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Cross Reactions
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Enzyme-Linked Immunosorbent Assay
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Female
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Human
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Korea/epidemiology
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Larva Migrans, Visceral/*epidemiology/immunology
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Male
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Middle Aged
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Seroepidemiologic Studies
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Support, Non-U.S. Gov't
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Toxocara/immunology
4.Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing.
Ji Sun JANG ; Kyung Ho KIM ; Jae Ran YU ; Soo Ung LEE
The Korean Journal of Parasitology 2007;45(4):301-306
We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.
Aged
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Aged, 80 and over
;
Animals
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Ascaridida/genetics/isolation & purification
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Ascaris lumbricoides/genetics/isolation & purification
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Base Sequence
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Clonorchis sinensis/genetics/isolation & purification
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Common Bile Duct/*parasitology
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DNA, Helminth/*genetics
;
DNA, Ribosomal/genetics
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Face/parasitology
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Female
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Gallbladder/parasitology
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Gallstones/*parasitology
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Helminths/genetics/*isolation & purification
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Humans
;
Male
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Middle Aged
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Molecular Sequence Data
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Polymerase Chain Reaction/*methods
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RNA, Ribosomal, 18S/genetics
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RNA, Ribosomal, 28S/genetics
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Sequence Alignment
5.Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai.
Soo Ung LEE ; Sun HUH ; Woon Mok SOHN ; Jong Yil CHAI
The Korean Journal of Parasitology 2004;42(3):129-135
We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.
Animals
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Base Sequence
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Comparative Study
;
DNA, Helminth/*chemistry/genetics
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DNA, Mitochondrial/chemistry/genetics
;
DNA, Ribosomal/chemistry/genetics
;
Electron Transport Complex IV/chemistry/*genetics
;
Heterophyidae/classification/enzymology/*genetics
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 28S/chemistry/*genetics
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Research Support, Non-U.S. Gov't
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Sequence Alignment
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Trematode Infections/*parasitology
6.A Case of Turner's Syndrome Associated with Atrial Septal Defect and Mitral Valve Prolapse.
Jae Ung LEE ; Kyung Soo KIM ; Jeong Hyun KIM ; Heon Kil LIM ; Bang Hun LEE ; Chung Kyun LEE
Korean Circulation Journal 1995;25(4):875-879
We experience a case of 34-year-old Turner's syndrome(45, XO) associated with atrial septal defect(secondum type) and mitral valve prolapse who was admitten due to moderate exertional dyspnea. It is well know hat chromosomal abnormality is one of the etiology of congenital heart disease. In case of Turner's syndrome, coarctation of aorta or bocuspid aortic valve is frequently combined, but atrial septal defect simultaneously with mitral valve prolapse is not reported till now in Krea. We report this case with a brief review of the literature.
Adult
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Aortic Coarctation
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Aortic Valve
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Chromosome Aberrations
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Dyspnea
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Heart Defects, Congenital
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Heart Septal Defects, Atrial*
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Humans
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Mitral Valve Prolapse*
;
Mitral Valve*
;
Turner Syndrome*
7.Hemodynamic Effects of Intravenous Enoximone in Moderately Severe Congestive Heart Failure.
Jeong Hyun KIM ; Jae Ung LEE ; Kyung Soo KIM ; Heon Kil LIM ; Bang Hun LEE ; Chung Kyun LEE
Korean Circulation Journal 1995;25(3):684-690
BACKGROUND: This clinical trial was performed to evaluate the hemodynamic and side effects of enoximone, a newly developed phosphodiesterase inhibitor, in moderately severe congestive heart failure in Korean population and to base the development of long acting oral preparations in the future. METHODS: Principal admission criteria for this trial were a left ventricular ejection fraction of less than 45% by radionuclide ventriculography, NYHA functional class II or III and a documented congestive cardiomyopathy. Exclusion criteria were restrictive cardiomyopathy, valvular heart disease, multisystemic lillness and uncontrolled ventricular tachyarrhythmia. The hemodynamic measurements were made by using the thermodilution Swan-Ganz catheter and radial arterial canulation. Enoximone was administered(continuous infusion) for 24 hours after initial bolus. The hemodynamic parameters to be observed were blood pressure, heart rate, cardiac output, pulmonary capillary wedge pressure, systemic and pulmonary vascular resistance. The clinical reponse, hematology, blood chemistry, and Holter monitoring were checked before and after enoximone trial. RESULTS: The following results are obtained. 1) The included patients were 15 females, 24 males, total 39 patients and mean age of 55.3 years old(28-70 years old). 2) The causes of congestive cardiomyopathy were idiopathic 35(89.9%), hypertensive 3(7.7%), and alcoholic 1(2.6%). 3) The mean ejection fraction measured by radionuclide left ventriculography were 28. 6%. 4) THe cardiac output was significantly increased(50%), and pulmonary capillary wedge pressure(38%), systemic vascular resistance(34%), pulmonary vascular resistance(27%) were significantly decreased during enoximone infusion. 5) NYHA Functional Class was improved by 1 step with enoximone. 6) There were no significant changes in hematology, blood chemistry, and Holter monitoring with enoximone. CONCLUSION: From the above results, the short term intravenous enoximone was very effective in moderately severe congestive heart failure in congestive cardiomyopathy without major adverse effects in Korean.
Alcoholics
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Blood Pressure
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Capillaries
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Cardiac Output
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Cardiomyopathy, Dilated
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Cardiomyopathy, Restrictive
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Catheters
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Chemistry
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Electrocardiography, Ambulatory
;
Enoximone*
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Estrogens, Conjugated (USP)*
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Female
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Heart Failure*
;
Heart Rate
;
Heart Valve Diseases
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Hematology
;
Hemodynamics*
;
Humans
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Male
;
Pulmonary Wedge Pressure
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Radionuclide Ventriculography
;
Stroke Volume
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Tachycardia
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Thermodilution
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Vascular Resistance
9.Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidae).
The Korean Journal of Parasitology 2006;44(3):233-237
We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.
Spirometra/*genetics/growth & development/physiology
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Molecular Sequence Data
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Gene Library
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Cloning, Molecular
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Caspases/genetics
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Caenorhabditis elegans Proteins/genetics
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Base Sequence
;
Apoptosis/*genetics
;
Animals
;
Amino Acid Sequence
10.Clinical Considerations of Convulsions in Children.
Whi Dai KIM ; Soo ung LEE ; Joong sik KIM ; Chang Il AHN
Journal of the Korean Pediatric Society 1977;20(1):50-53
Total of 311 cases who were admitted to pediatric departmenr of Kyung Hee University Hospital from October, 1971 to December, 1975 were studied clinically about the cases in various age group. The results are as follows. 1) Convulsion was most frequent in children between 6months and 3years (38%), but it was least frequent in children from 10years to 15 years (7%). 2) The most common cause of convulsions in children was febrile convulsion(30.5%). 3) Tetanus (50.9%) was most frequent cause of convulsion in the neonatal period. 4) Febtile convulsion was most common in infants from one month to six months. 5) The most common cause of conculsions in infants from six months to three years was febrile convulsion (47.8%). 6) In children more than three years of age, idiopathic epilepsy was most frequent cause of convulsion (3 to 10 years and 10 years to 15years , 36%, 72.7% respectively).
Child*
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Epilepsy
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Humans
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Infant
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Seizures*
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Seizures, Febrile
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Tetanus