1.Contact Dermatitis to Isoconazole Nitrate.
Soo Chan KIM ; Yeol Oh SUNG ; Won Soo LEE
Annals of Dermatology 1989;1(1):43-45
No abstract available.
Dermatitis, Contact*
2.A Case of Eccrine Chromhidrosis Caused by Silver lodide.
Won Soo LEE ; Yeol Oh SUNG ; Jung Bock LEE
Korean Journal of Dermatology 1989;27(6):724-728
Eccrine chromhidrosis(colored sweating), resulting from the contamination of the sweat by dyes, pigment from microorganisms such as those of Piedraia or Corynebacterium, or other chernicals, is a very rare disease. We report a case of eccrine chromhidrosis which revealed dark-brown colored pigmented spots analyzed by energy dispersive X-ray microassay and scanning electron microscopy, which were thought to be composed of silver iodide(AgI) formed by the conjugation of silver components from outside and iodide components of eccrine sweat, on the face, postauricular area, legs, and finger tips.
Coloring Agents
;
Corynebacterium
;
Fingers
;
Leg
;
Microscopy, Electron, Scanning
;
Rare Diseases
;
Silver*
;
Sweat
3.A Case of Rapidly Progressive Glomerulonephritis in Henoch-Schonlein Purpura.
Soo Hee CHANG ; Dae Sun JO ; Dae Yeol LEE
Journal of the Korean Society of Pediatric Nephrology 1997;1(1):82-85
A case of primary non-clear-cell adenocarcinoma of the vagina is reported occurring in a 65-year-old woman without exposure to diethylstilbestrol (DES) in utero. The adenocarcinoma did not appear to be associated with vaginal adenosis. It lacked clear cell component and interestingly composed of columnar epithelial cells of endocervical-type. Cytologically round to oval nuclei revealed one or more small nucleoli and fine granular chromatin pattern. Cytoplasm was plump, faintly basophilic and homogeneously stained. Histologically well differentiated columnar epithelial cells were arranged in trabecular pattern mainly, and also occasional glandular lumina and small solid sheets were found. Mitoses were hardly found.
Adenocarcinoma
;
Aged
;
Basophils
;
Cellular Structures
;
Chromatin
;
Cytoplasm
;
Diethylstilbestrol
;
Epithelial Cells
;
Female
;
Glomerulonephritis*
;
Humans
;
Mitosis
;
Purpura, Schoenlein-Henoch*
;
Vagina
4.Clinical Observation of Congenital Urinary Tract Anomalies.
Sun Jun KIM ; Dae Yeol LEE ; Soo Hee CHANG
Journal of the Korean Society of Pediatric Nephrology 1997;1(1):67-72
We present the cytologic features of a case of solid and papillary neoplasm of the pancreas. Cytologically, the tumor was composed of a monotonous population of polygonal cells containing eccentrically located round nuclei with one or two distinct small nucleoli and a finely stippled chromatin pattern. The tumor cells were similar to those of the islet cell tumor and showed isolated loosely aggregated and solid sheets or large cell clumps. The large cell clumps revealed a branching papillary structure containing fibrovascular central core, which is characteristic histologic feature of solid and papillary neoplasm of the pancreas. This case was confirmed by tissue examination including histochemical, immunohistochemical and electron microscopical studies. Ultrastructurally, the tumor cells contained a few membrane-bound electron dense granules.
Adenoma, Islet Cell
;
Chromatin
;
Pancreas
;
Urinary Tract*
6.ENDOSCOPIC ASSISTED BROW-FOREHEAD LIFTING.
Myung Ju LEE ; Bong Soo RHU ; Jeong Yeol YANG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(2):401-413
Aging process of the forehead in human reflects imbalance of the forehead muscles, forehead ptosis including eyebrow, and winkles of the forehead and glabella. Forehead lifting through bicoronal incision was adopted as an universal method to correct these problems of the aging process. In a view of the aesthetic surgery, however, there are several problems such as large scar alopecia, and numbness beyond the incision line in the scalp for the correction of the forehead aging stigma. During the part few decades, minimally invasive technology forced many surgical specialities to undergo dramatic changes. In plastic & reconstructive surgery also minimally invasive technology using endoscope gave many advantages. Therefore it has reduced morbidity rates and got greater patient acceptances for the forehead lifting. When we perform endoscopic blow-forehead lift to get a good results in oriental people, 1. it needs a extended periorbital dissection. 2. we have to concern lift in the lateral part of forehead rather than in medial forehead. 3. it needs more aggressive tissue modifications (e.g. myotomies, neurotomy) 4. it needs more strong fixation such as permanent microscrew fixation or cortical bone drill hole technique. We performed 13 cases of the eyebrow-forehead lifting by using endoscope from August 95 to September 96 followed up them from 3 to 12 months and obtained satisfactory result. We would like to report our experiences with literature review.
Aging
;
Alopecia
;
Cicatrix
;
Endoscopes
;
Eyebrows
;
Forehead
;
Humans
;
Hypesthesia
;
Lifting*
;
Muscles
;
Plastics
;
Scalp
7.Expression of Metallothionein in the Neoplasms of Ectodermal Origin.
Ki Ho KIM ; Min Soo LEE ; Gwang Yeol JOH
Korean Journal of Dermatology 1997;35(1):107-113
BACKGROUND: Metallothioneins(MTs) are ubiquitous low molecular weight cytosolic proteins with high contents of cystein that xhibit a selective binding affinity for zinc, copper and other group II heavy metal ions. In additi~on to the sequestration function and detoxification action, MTs probably participate in the cellirlar protection against. ionizing radiation and alkylating agent cytotoxicity and in cellular pn:liferation. OBJECT: The objective of this study was to evaIuate the expression of MTs and to clarify t.heir role in carcinogenesis and/or biological behaviour in the neoplasms of ectoderrnal origin. METHODS: We performed immunohistochemical studies on the surgical specimens of rnalignant rnelanoma and squamous cell carcinoma and basal cell carcinoma. The results were compared with the normal skin and mu osa and hair t,issues and raf't-on culture tissues of skin and mucosal keratinocytes. RESULTS: 1) MTs were strongly expressed in epidermal basal cells of the normal skin and raft-on cultured artificial skin They were also rnore weakly expressed in epithelial basal cells of the normal mucosa and raft on cultured artificial mucosa and in matrix cells of the normal hair. 2) In rnalignant melanoma, MTs were meaningfully expressed, i.e., a strong expression was seen in the lesions showing a rapid vertical growth phase with fatal out,comes, but a weak expression in the cases whose precursor lesions were thought as dysplastic nevi, compared to the negative finding of norrnal melanocyt,es. The strong expression in a case of congenit.al rnelanocyt.ic nevus is considered as the activation of nevoid melanocytes. 3) MTs were weakly expressed in squarnous cell carcinoma with a diffuse aattern within the tumor mass. St,rong expression of MTs was particularly apparent in the poorl. differentiated squamous cell carcinoma and at the growing border of well differentiated squamous cell carcinoma. 4) In basal cell carcinoma, MTs were rather weakly expressed in solid cireumscribed types with a diffuse pattern and in cystic type with a patchy pattern. However they were not expressed in the solid infiltrative type. CONCLUSION: From these resuilts, we concluded that MTs were ubiquitously present in possible stem cells of various epithelial tissues except melanocytes irrespective of normal or artificial skin and/or mucosa including norrnal hair tissues. In the neoplasms of ectodermal origin, MTs might be involved in the invasive growth of tumor cells in the malignant melanoma and squamous cell carcinoma. It was implied that the role of MTs in basal cell carcinoma might be related to early events of hasal cell carcinogeresis and its down regulating tendency could be possible in its infil trative stage.
Carcinogenesis
;
Carcinoma, Basal Cell
;
Carcinoma, Squamous Cell
;
Copper
;
Cytosol
;
Dysplastic Nevus Syndrome
;
Ectoderm*
;
Hair
;
Ions
;
Keratinocytes
;
Melanocytes
;
Melanoma
;
Metallothionein*
;
Molecular Weight
;
Mucous Membrane
;
Nevus
;
Radiation, Ionizing
;
Skin
;
Skin, Artificial
;
Stem Cells
;
Zinc
8.Ca Effects on Synthesis and Secretion of Insulin-like Growth Factor(IGF-I) and IGF-Binding Proteins by the Perfased Rat Liver
Dae Yeol LEE ; Chang Won KANG ; Jung Soo KIM
Journal of Korean Society of Endocrinology 1996;11(2):189-198
Background: The insulin-like growth factors, IGF-I and
Animals
;
Calcimycin
;
Calcium
;
Egtazic Acid
;
Extracellular Fluid
;
Glucose
;
Hepatocytes
;
Insulin
;
Insulin-Like Growth Factor Binding Protein 3
;
Insulin-Like Growth Factor Binding Proteins
;
Insulin-Like Growth Factor I
;
Liver
;
Metabolism
;
Parathyroid Hormone
;
Perfusion
;
Rats
;
Somatomedins
9.Characteristics of Serum Insulin-like Growth Factor ( IGF ) and IGF-Bindign Protein-3 during Pregnancy.
Dae Yeol LEE ; Jung Soo KIM ; Hong Ro LEE ; Cheol Hee RHEE ; Soo Chul CHO
Journal of Korean Society of Endocrinology 1997;12(3):376-385
BACKGROUND: Pregnancy in human and rodents is associated with dramatic matemal metabolic changes. Insulin-like growth factors (IGFs) are mitogenic peptides that are essential for fetal and maternal tissue growth during pregnancy. They circulate complexed primarily with a serum IGF-binding protein (IGFBP-3) which regulates the availability of the IGFs to their specific target tissues. METHODS: To examine the changes of IGFs and IGFB-3 during pregnancy, we measured serum total IGF-I, free IGF-I, IGF-II and IGFBP-3 by using specific radioimmunoassay, immunoradio-metric assay, western ligand blot and western immunoblot. Blood samples were obtained from 88 pregnant women between 6-40 weeks gestation. RESULTS: While serum IGF-I levels increased up to 50% in late pregnancy, serum IGF-II levels remained unchanged. However, serum free IGF-I levels were significantly higher during pregnancy than in nonpregnancy. Western ligand blot analysis revealed that IGFBP-3 in pregnancy serum was significantly decreased at 6 weeks of gestation, continued decreased level until term, and returned to a nonpregnant level by postpartum 10 day. Serum IGFBP-3 profiles in Western immunoblot analysis revealed that 30 kDa fragments of IGFBP-3 were detectable in pregnancy serum but not in nonpregnancy serum. In contrast, serum IGFBP-3 levels using radioimmunoassay was significantly increased in late pregnancy. CONCLUSIONS: 1) serum IGF-I was significantly elevated in late pregnancy 2) serum IGF-II was not significantly changed 3) free IGF-I significantly elevated throughout gestation 4) intact IGFBP-3 was markedly reduced after 6 weeks of gestation.
Blotting, Western
;
Female
;
Humans
;
Insulin-Like Growth Factor Binding Protein 3
;
Insulin-Like Growth Factor I
;
Insulin-Like Growth Factor II
;
Peptides
;
Postpartum Period
;
Pregnancy*
;
Pregnant Women
;
Radioimmunoassay
;
Rodentia
;
Somatomedins
10.Survival, Differentiation and ATM Phenotype of PC-12 Cells by Down - regulation of AT Gene.
Ho Keun YI ; Soo Hee CHANG ; Dae Yeol LEE ; Jung Soo KIM ; Pyoung Han HWANG
Journal of the Korean Cancer Association 1999;31(5):1065-1073
PURPOSE: Ataxia Telangiectasia (AT) is a hereditary multi-systemic disease resulting from mutations of AT gene and is characterized by progressive neurodegeneration, cancer, immune system defects, and hypersensitivity to ionizing radiation. AT gene has a homologue sequence of PI3-kinase. The activity and cellular function of PI3-kinase in AT gene remains unclear. This study was undertaken to evaluate the function of AT gene through the effect on cell survival and differentiation by the inhibition of AT gene expression. MATERIALS AND METHODS: NH2-terminal portion of AT gene was isolated from MCF-7 cells by RT-PCR. The isolated DNA fragment was ligated in reverse orientation in pcDNA3. This antisense ATM expression vector was transfected to PC-12 cells by calcium phosphate method, and the transformed cells were selected using G418 and immunohisto- chemistry. To analyze the cell survival and differentiation, cells were cultured in serum free medium supplemented with/without NGF. We performed the immunoprecipitation for the p53 induction of cells after ionizing radiation, and the FACS for the apoptosis of cells after the exposure of wortmanin. RESULTS: PC-12 cells which down-regulated AT gene (like ATM, AT mutated) showed decreased survival and ceased differentiation with NGF. Also, PC-12 (ATM) cells showed increased apoptosis with wortmanin and reduced or delayed p53 induction after ionizingradiation. CONCLUSION: Results obtained from these studies suggest that AT gene regulates survival and differentiation of PC-12 cells through PI3-kinase activity. It seems that apoptosis is induced by the inhibition of AT gene expression.
Apoptosis
;
Ataxia Telangiectasia
;
Calcium
;
Cell Survival
;
Chemistry
;
DNA
;
Gene Expression
;
Hypersensitivity
;
Immune System
;
Immunoprecipitation
;
MCF-7 Cells
;
Nerve Growth Factor
;
Phenotype*
;
Phosphatidylinositol 3-Kinases
;
Radiation, Ionizing