No abstract available.
Genetic Therapy*

No abstract available.
Ethics*

Occupational asthma induced by the inhalation of the spore of Ganoderma lucidum was demonstrated in a 43-year-old woman who had cultivated it indoors for 6 years. She had suffered from paroxysmal cough and rarely wheezing dyspnea for two years. Recently she also had chest tightness and paroxysmal and repeated cough immediately after the exposure to Ganoderma lucidum spores. Her pulmonary function was normal. But, her PC20 was 6.4mg/ml in MBPT (methacholine bronchial provocation test). She showed not only positive reaction to skin prick test but also dual asthmatic reaction to the bronchial challenge test with antigenic extract of Ganoderma lucidum spores.
Adult ; Asthma, Occupational* ; Bronchial Provocation Tests ; Cough ; Dyspnea ; Female ; Ganoderma* ; Humans ; Inhalation ; Reishi ; Respiratory Sounds ; Skin ; Spores* ; Thorax

To evaluate the utility of gene rearrangement analysis, eight cases of malignant lymphoma, one case of Hodgkin's disease, two cases of angioiminunoblastic lymphadenopathy (AILD) and two cases of non-specific lymphadenitis were studied by immunohistochemical and genetic analysis. Southern blot analysis was perfon-ned by a using vacuum transfer system and a biotin labelled probe. This method was faster, safer, and more convenient than conventional methods. Gene rearrangement study showed rearranged novel bands in five of six cases of B cell lymphoma, in all cases of T cell lymphoma, and in all cases of AILD. No rearrangement of the B cell receptor(BCR) or of the T cell receptor(TCR) was seen in Hodgkin's disease or in nonspecific lymphadenitis. These results suggest that gene rearrangement analysis of BCR and TCR is a recommended method for the diagnosis of clonality in lymphoproliferative disorders. It would allow pathologists to differentiate lymphoma from polyclonal lymphoid proliferation and to provide information for cell lineage.

Mycobacteria cause diseases which occur the world over and which carry a considerable burden in morbidity, mortality and social problems. A battery of monoclonal antibodies specific for mycobacterial antigens would provide a useful tool for rapid diagnosis of mycobacterial diseases. Fourteen monoclonal antibodies to perchloric acid soluble antigen of M. tuberculosis were produced. Immunoglobulin isotypes of monoclonal antibodies were ten of immunoglobulin G2a, two of IgG3, and two of IgM. By means of Western blotting, monoclonal antibody detected the antigen of 54kD in TB-P. In the immunofluorescence assay, the monoclonal antibody showed a positive reaction with intact M. tuberculosis bacilli, M. tuberculosis in the pulmonary tissue of tuberculous patient and M. bovis BCG.
Antibodies, Monoclonal* ; Blotting, Western ; Diagnosis ; Fluorescent Antibody Technique ; Humans ; Immunoglobulin G ; Immunoglobulin Isotypes ; Immunoglobulin M ; Immunoglobulins ; Mortality ; Mycobacterium bovis ; Social Problems ; Tuberculosis

No abstract available.
Mycobacterium bovis*

A 25-year-old male with asthma suffered from generalized urticaria, angioedema and syncope associated with exercise after ingestion of food made with wheat flour. Skin prick test with bread showed strong positive reaction(++++), but with wheat flour, a weak positive(+). Challenge test with wheat flour, and food made with wheat flour, without or with exercise, were done several times. Exercise such as 25 min of running on treadmill following 30 min after 50 grams of bread or 50 grams of hot pepper-stuffed rice cake only showed positive reaction such as generalized urticaria, angioedema and hypotension, but not with 15 grams of bread or 15 grams of hot pepper-stuffed rice cake. Ingestion of bread in such high amounts as 15 grams alone did not trigger reaction, not even with is min of exercise. This is the first clear case of food dependent exercise induced anaphylaxis (FDEIA) in which the allergic reaction depended on both the amount of food ingested and the amount of exercise was clarified.
Adult ; Anaphylaxis* ; Angioedema ; Asthma ; Bread ; Eating ; Flour* ; Humans ; Hypersensitivity ; Hypotension ; Male ; Running ; Skin ; Syncope ; Triticum* ; Urticaria

PURPOSE: The aim of this study was to find correlation between the expression of epidermal growth factor receptor (ECFR) and MR findings in the brain glioma. MATERIALS AND METHODS: MR features including edema, margin, necrosis, heterogeneity, hemorrhage and contrast enhancement were retrospectively analyzed with preoperative MR images in 41 patients with proven brain gliomas (8 low grade astrocytomas, 12 anaplastic astrocytomas, 21 glioblastoma multiformes). Immunohistochemical study of EGFR was done and their expressions were graded by both stained distribution and intensity. Correlation analysis between the MR features and EGFR expressions was done. RESULTS: Peritumoral edema was correlated with both distribution (r=0.71, p=0.00) and stain intensity (r=0.69, p=0.00) of EGFR expression. Other MR features showed no statistical correlation with EGFR expression. CONCLUSION: MRI is useful in evaluation of brain glioma, and peritumoral edema is useful findings that suggests EGFR expression as well as malignant histopathologic grade of the tumor.
Astrocytoma ; Brain ; Edema ; Epidermal Growth Factor* ; Glioblastoma ; Glioma* ; Hemorrhage ; Humans ; Magnetic Resonance Imaging ; Necrosis ; Population Characteristics ; Receptor, Epidermal Growth Factor* ; Retrospective Studies

Herpes zoster is a acute viral infection of nerve structures manifesting cutaneous lesions in the form of groups of vesicles distributed along one or more peripheral sensory nerves. Sometimes involve motor nerves. The skin lesions usually appear on unilateral, but some on bilateral. And caused by variella-zoster virus, which is the same virus, with varicella. The most important etiologic mechanism is the reactivation of the latent virus; a latent virus infection is set up in spinal and cranial sensory ganglion as a result of hematogenous dissemination during the initial varicella infection and is activated in later life so that the virus spread down to the peripheral nerve into the skin. Durig last 5 years (1968-1972) 94 cases of Herpes zoster, of Dermatology Dept. in N.M.C. were investigated by statistics of sex, age, predilection site of skin eruptions, seasonal variation, and of its complications or associated diseases. The reults were following; In sex, age distribution, female is rather common than male and more commonly affected after 40's. In predilection site, more than half cases, involved thoracic cutaneous nerves, and the least were sacral and lateral cutaneous nerves. Right and left side proportion was about same (40: 49), bilateral or mid-zone involvement were 5 cases. In seasonal distribution, there was no relation between epidemic of varicella and Herpes zoster, The most were at Summer, and the least at Autumn. In complications and associated diseases, complication noted in 4 cases, 3 were Herpes zoster ophthalmicus and 1 case was Ramsey-Hunt syndrome. The most frequent associating disease was pulmonary tuberculosis, otherwise were diabetes, asthma etc.
Age Distribution ; Asthma ; Chickenpox ; Dermatology ; Female ; Ganglia, Sensory ; Herpes Zoster Ophthalmicus ; Herpes Zoster* ; Humans ; Male ; Peripheral Nerves ; Seasons ; Skin ; Tuberculosis, Pulmonary