1.Porcine Granulosa Cells Produce Gonadotropin Surge Inhibiting Factor (GnSIF) Activity In Vitro.
Korean Journal of Obstetrics and Gynecology 1999;42(12):2815-2820
OBJECTIVES: It has been identified a novel protein in porcine and bovine follicular fluid termed Gonadotropin Surge Inhibiting Factor (GnSIF) which inhibits GnRH-stimulated LH secretion by pituitary cells in culture. The purpose of this study was to investigate the production of GnSIF by porcine granulosa cell in vitro. MATERIALS AND METHODS: Granulosa cells were aspirated from different size of porcine follicles (1-3mm, 3-5mm, >6mm in diameter) and cultured in 24-well plates in Ham's F-12/DMEM at 500,000 cells/well for 24h. After plating, cells were washed and incubated in triplicate for 72h in DMEM/F-12 containing 2% FBS in the presence of 100ng/ml FSH and the media were collected. Granulosa cell-conditioned media were chromatographed on heparin-Sepharose to remove inhibin and follistatin, and assayed for GnSIF activity using a rat pituitary cell bioassay. Briefly, dispersed pituitary cells from adult female Sprague-Dawley rats were plated (500,000 cells/well) for 72h, washed, and incubated with granulosa cell-conditioned media in the presence of 10nM GnRH for 4h. The media were collected and assayed for LH by RIA. GnSIF activity is defined as the suppression of GnRH-stimulated LH secretion. RESULTS: Granulosa cell-conditioned media significantly inhibited GnRH-stimulated LH secretion by rat pituitary cells in vitro (69% of control, P<0.001, n=2 experiments). Preliminary data suggest that granulosa cells from small follicles (1-3mm) produce greater amounts of GnSIF than those obtained from larger follicles (>6mm). CONCLUSIONS: GnSIF activity is produced by porcine granulosa cells in vitro. Granulosa cell production of GnSIF, along with other gonadal proteins such as inhibin, follistatin, and activin may be an important component of the ovarian control of pituitary gonadotropin secretion.
Activins
;
Adult
;
Animals
;
Biological Assay
;
Female
;
Follicular Fluid
;
Follistatin
;
Gonadotropin-Releasing Hormone
;
Gonadotropins*
;
Gonads
;
Granulosa Cells*
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Humans
;
Inhibins
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Rats
;
Rats, Sprague-Dawley
2.Autoradiographic studies on some parasitic helminth.
Joo Soo YOON ; Soon Hyung LEE ; Byong Seol SEO
The Korean Journal of Parasitology 1964;2(3):165-169
Direct contact method of autoradiography was utilized in studying the distribution of exogenous C(14)-proline in Clonorchis sinensis, Eurytrema pancreaticum, Hymenolepis diminuta and Dipylidium caninum. The most distinct autoradiogram were obtained after 60 min of incubation, corresponding to the maximal absorption of C(14)-proline in these parasite. The radioactivitity of this labeled amino acid was chiefly concentrated in reproductive organs, especially egg-containing uterine tubules.
parasitology-helminth-trematoda-cestoda
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Clonorchis sinensis
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Eurytrema pancreaticum
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Hymenolepis diminuta
;
Dipylidium caninum
;
autoradiography
;
C(14)-proline
;
biochemistry
;
amino acid
3.A Case of Heterotopic Pregnancy in Natural Cycle.
Sung Wook SONG ; Young Whan JUNG ; Zong Soo MOON ; Soo Hyung SEO ; Yang Suh PARK
Korean Journal of Perinatology 2001;12(2):188-192
No abstract available.
Pregnancy, Heterotopic*
4.CT and MR findings of mycotic infection of the paranasal sinus: differentiation from sinonasal neoplasm.
Kil Woo LEE ; Hyo Keun LIM ; Gwy Suk SEO ; Suk Soo BAE ; Shin Hyung LEE
Journal of the Korean Radiological Society 1992;28(2):176-181
When a soft tissue mass in the bony wall of the paranasal sinus is present, it is difficult to make a distinction between tumor and inflammatory mass on CT. Fungal sinusitis may have soft tissue attenuation on the bony wall of the sinus, bony sclerosis, focal bony destruction, and calcific area on CT. This is a report of four proven cases of fungal sinusitis, asperogillosis in 3 cases and mucormycosis in 1 case, All 4 patients had CT and one patient had MRI, On CT, bony sclerosis and destruction were well visualized in all cases. On MRI, mycetoma in the maxillary sinus was hypointense on T1 weighted images and more hypointense on T2 weighted images. Although CT appears to be the best modality for initial examination of the patient with sinusitis, the differentiation of fungal sinusitis from tumor mass or other entity may be better accomplished with MRI.
Humans
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Magnetic Resonance Imaging
;
Maxillary Sinus
;
Mucormycosis
;
Mycetoma
;
Sclerosis
;
Sinusitis
5.Two Case of Watson-Alagille Syndrome.
Heon Seok HAN ; Soo Heum LIM ; Young Sun KIM ; Jeong Kee SEO ; Hyung Ro MOON
Journal of the Korean Pediatric Society 1985;28(10):1047-1051
No abstract available.
6.Hereditary Hemorrhagic Telangiectasia with Pulmonary Arteriovenous Fistula.
Heui Jeen KIM ; Young Soo YOON ; Jeong Kee SEO ; Hyung Ro MOON ; Kyung Mo YEON
Journal of the Korean Pediatric Society 1984;27(4):390-394
No abstract available.
Arteriovenous Fistula*
;
Telangiectasia, Hereditary Hemorrhagic*
7.Combined intrauterine and extrauterine gestation : a review.
Soo Hyung SEO ; Young Woo SHIN ; Tae Seung CHO ; Dong Han BAE
Korean Journal of Obstetrics and Gynecology 1993;36(7):2131-2139
No abstract available.
Pregnancy*
8.Dermoscopic Finding of Angioma Serpiginosum and Treatment.
In Hyuk KWON ; Tae Hyung RYU ; Soo Hong SEO ; Hyo Hyun AHN ; Hwa Jung RYU
Korean Journal of Dermatology 2016;54(8):669-670
No abstract available.
Dermoscopy
;
Hemangioma*
;
Lasers, Dye
9.A Case of Niemann-Pick Disease with Sea-Blue histiocytes in the Bone Marrow.
Young Sun KIM ; Soo Heum LIM ; Jeong Kee SEO ; Hyo Seop AHN ; Hyung Ro MOON
Journal of the Korean Pediatric Society 1985;28(12):1238-1244
No abstract available.
Bone Marrow*
;
Histiocytes*
;
Niemann-Pick Diseases*
10.Relationships among Ambulatory Plasma Renin Activity, Blood Pressure and Urinary Microalbumin Excretion Rate in Essential Hypertension.
In Soo PARK ; Ji Won PARK ; Bo In LEE ; Jae Yul SEO ; Jae Hyung KIM ; Soon Jo HONG
Korean Circulation Journal 1996;26(3):688-695
OBJECTIVE AND METHODS: To determine correlations among ambulatory renin activity, ambulatory blood pressure and microalbumin excretion rate, 66 Korean essential hypertensives were studied after 4 week wash-out period. The ambulatory blood pressure was monitored every 30 minutes and mean BP were calculated automatically. Urinary microalbumin excretion rate(UAER) and ambulatory plasma renin activity(aPRA) collected at mid-day were measured by radioimmunoassays. Subjectives were divided into 2 groups by aPRA value(2ng/ml/hr). RESULT: 14 cases were high renin group and 52 cases low renin group. The mean BP were 148.83/94.69mmHg in low renin group, and 146.57/98.07mmHg in high-renin group without difference. UAER were not different also between both groups. 23.07%(4/14) of non-dippers were included in high renin group and 25.58%(12/52) in low renin group without statistical difference. The aPRA was significantly related to UAER and systolic and diastolic mean blood pressure. Also UAER was related significantly to day mean blood pressures. CONCLUSION: Thus aPRA is thought to be a meaningful indicator to predict hypertensive renal target organ damage as well as blood pressure measured with 24-hr ABPM.
Blood Pressure*
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Hypertension*
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Plasma*
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Radioimmunoassay
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Renin*