No abstract available.
Fertilization in Vitro* ; Fertilization*

No abstract available.

No abstract available.
Animals ; Evoked Potentials, Motor* ; Rats*

PURPOSE: The objective of this study was to find the predictors and generate a prediction scoring model of nonresponse to intravenous immunoglobulin in patients with Kawasaki disease. METHODS: We examined 573 children diagnosed with KD at the Severance Children's Hospital between January 2009 and december 2012. We retrospectively reviewed their medical records. These patients were divided into 2 groups; the experimental group (N=433) and the validation group (N=140). Each group were divided into 2 groups the intravenous immunoglobulin nonresponders and the responders. Multivariate logistic regression analysis identified predictive factors of intravenous immunoglobulin nonresponders which make predictive scoring model. We practice internal validation and external validation. RESULTS: Multivariate logistic regression analysis identified male, cervical lymphadenopathy, changes of the extremities, platelet, total bilirubin, alkaline phophatase, lactate dehydrogenase, C-reactive protein as significant predictors for nonresponse to intravenous immunoglobulin. We generated prediction score assigning 1 point for (1) male, (2) cervical lymphadenopathy, (3) changes of the extremities, (4) platelet (< or =368,000/mm3), (5) total bilirubin (> or =0.4 mg/dL), (6) alkaline phophatase (> or =227 IU/L), (7) lactate dehydrogenase (> or =268 IU/L), (8) C-reactive protein (>77.1 mg/dL). Using a cut-off point of 4 and more with this prediction score, we could identify the intravenous immunoglobulin nonresponder group. Sensitivity and specificity were 52.5% and 82.4% in experimental group and 37.8% and 81.8% in validation group, respectively. CONCLUSION: Our predictive scoring models had high specificity and low sensitivity in Korean patients. Therefore it is useful in predicting nonresponse to intravenous immunoglobulin with Kawasaki disease.
Bilirubin ; Blood Platelets ; C-Reactive Protein ; Child ; Extremities ; Humans ; Immunoglobulins* ; Korea ; L-Lactate Dehydrogenase ; Logistic Models ; Lymphatic Diseases ; Male ; Medical Records ; Mucocutaneous Lymph Node Syndrome* ; Retrospective Studies ; Risk Factors ; Sensitivity and Specificity

No abstract available.
Nerve Regeneration* ; Silicones*

No abstract available.
Gallstones* ; Prevalence*

Recently many studies have shown the usefulness of computed tomogram in diagnosing abdominal mass when clinical and conventional radiologic examinations fail to reveal the nature of abdominal mass or the cause of abdominal distension. To evaluate the usefulness of CT in diagnosing neuroblastoma, we retrospectively analyzed computed tomographic findings of 16 neuroblastoma patients, who pathologically proved in Yeungnam University Hospital from 1986 to 1995. The age range of the patients studied were from 8months to 18years. The most frequent sith of origin was adrenal gland and the next was retroperitioneum. The presenting symptoms were palpable mass, abdominal distension, and abdominal pain.- The viewpoints of this analysis were tumoral calcifications, midline cross, shape, margin, internal structure, contrast enhancement patterns, major vessel involvement, and lymph node involvement. ':haracteristic CT findings were 'as follows: Fine dense curvillinear calcification within the tumor(56%), midline cross(50%), lobulation(75%), well-circumscribed margin(56%), cystic degeneration(56%), heterogeneous contrast enhancement(690/o), encasement of major vessels such as aorta, IVC and celiac trunk(50%), and paraaortic lymphadenopathy(87%). We conclude that these CT findings were very common and could be helpful in diagnosting and differentiation neuroblastoma in infant and children.
Adrenal Glands ; Aorta ; Child ; Diagnosis ; Humans ; Infant ; Lymph Nodes ; Neuroblastoma* ; Retrospective Studies

OBJECTIVE: To evaluate the impact of the cytologic smear of the endocervical canal immediately after cervical conization on the rate of residual cervical lesions. METHOD: A retrospective review of 229 patients who underwent cervical conization for high grade cervical intraepithelial neoplasia or microinvasive cancer followed by hysterectomy. Endocervical Papanicolaou smear with a cytologic brush performed immediately after cervical conization in all 229 patients. RESULTS: Fifty-four of 229 patients undergoing cervical conization followed by hysterectomy had residual lesion in the hysterectomy specimen. The prevalence rate of residual lesion according to the margin status of conization specimen as well as the results of the postconization Papanicolaou smear of the endocervical canal was compared. Forty-one of 78 patients (52.6 %) with positive margins had residual lesion compared with thirteen of 151 patients (8.6 %) with negative margins. Twenty-six of 34 patients (76.5 %) with positive postcone cytology had residual lesion compared with twenty-eight of 195 patients (14.4 %) with negative postcone cytology. CONCLUSIONS: Postcone cytologic smear of the endocervical canal as well as margin status are useful in predicting residual lesion after conization.
Cervical Intraepithelial Neoplasia ; Cervix Uteri ; Conization* ; Female ; Humans ; Hysterectomy ; Papanicolaou Test ; Prevalence ; Retrospective Studies

BACKGROUND: Dual esterase reaction enables simultaneous visualization of the cells exhibiting different characteristics by combining two esterase stains on a single slide. However, most methods previously reported require the smear slides to be stained twice, using separate coupling reagent with each substrate. Authors attempted to combine chloroacetate esterase (CAE) and alpha-naphthyl butyrate esterase (ANBE) staining methods by using single incubation and single mixed reagent. METHODS: For dual cytochemical reaction, buffy coat slides of normal subjects and bone marrow smear slides of patients with acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS) were used. Eighty mg of fast blue BB salt was dissolved in 50 mL of 0.1 mol/L phosphate buffer (pH 8.0). In the fast blue BB solution, 2 mL of acetone containing 4 mg of naphthol AS-D chloroacetate (Sigma, USA) and 8 mg of alpha-naphthyl butyrate (Sigma, USA) was added. Air dried slides were fixed with buffered formalin-acetone solution for 1 minutes. The fixed slides were allowed to be incubated with the single mixed reagent solution at room temperature for 20 minutes. Aquous hematoxylin dye was used for counterstaining. RESULTS: Granulocytes were stained bright blue by single-incubated dual esterase reaction. Monocytes and their precursors showed dark brownish positivity in cytoplasmic granules. Megakaryocytes in bone marrow revealed positive reaction for only ANBE by dual esterase method. The activity of CAE in myeloid lineage was markedly decreased in patients with MDS and some cells revealed dual staining activity with both punctate blue and brownish granules. A few neutrophils in karryorrhexis observed in remission marrow of AML patient showed strong positive reaction for CAE. CONCLUSIONS: Dual esterase staining method using single incubation is very simple in staining procedure and of use in simply identifying cellular characteristics and differentiation of myeloid and monocytic lineages on a single slide.
Acetone ; Bone Marrow ; Butyrates ; Coloring Agents ; Cytoplasmic Granules ; Granulocytes ; Hematoxylin ; Humans ; Leukemia, Myeloid, Acute ; Megakaryocytes ; Monocytes ; Myelodysplastic Syndromes ; Neutrophils

No abstract available.