Granulosa cell tumor of the testis is extremly rare in adult males and granulosa cell tumor occurring in the paratesticular area has not been reported. We report a paratesticular granulosa cell tumor in a 34-year-old man who presented with a 5.5 cm sized scrotal mass. The tumor was present in the paratesticular area near the head of epididymis. It was located in the tunica and completely separated from the testis by thick fibrous tissue. Microscopically, the tumor exhibited solid, microfollicular, and trabecular patterns. The tumor cells had ovoid to elongated nuclei with longitudinal intranuclear grooves and one or two nucleoli and scanty cytoplasm. Mitoses were relatively frequent with an average number of 9/10 HPFs. The tumor extended into the visceral tunica vaginalis and showed multiple lymphatic tumor emboli. Immunohisto chemical stains revealed diffuse strong positivity for inhibin, vimentin, and keratin and diffuse weak positivity for estrogen and progesteron receptor. Placental alkaline phosphatase (PLAP) and epithelial membrane antigen (EMA) were negative. On electron microscopic examination, tumor cells were polygonal and had large indented nuclei. The cytoplasm contained a moderate number of small round mitochondria, abundant rough and smooth endoplasmic reticula, and a few lipid droplets. Small aggregates of intermediate filaments and intercellular junctions were observed. The patient was alive and well 5 months after orchiectomy. This is the first case of adult granulosa cell tumor arising in the paratesticular area.
Adult ; Alkaline Phosphatase ; Coloring Agents ; Cytoplasm ; Epididymis ; Estrogens ; Female ; Granulosa Cell Tumor* ; Granulosa Cells* ; Head ; Humans ; Inhibins ; Intercellular Junctions ; Intermediate Filaments ; Male ; Mitochondria ; Mitosis ; Mucin-1 ; Orchiectomy ; Testis ; Vimentin

No abstract available.
Immunologic Tests* ; Tuberculosis, Meningeal*

No abstract available.

Pfeiffer syndrome, an unusual type of acrocephalosyndactyly, is a complex of associated malformations, first described by Pfeiffer in 1964. In addition to the common head and face anomalies seen in other acrocephalosyndactylies, its characteristics are broad thumbs and big toes, minimal syndactyly and normal intelligence. It is inherited in autosomal dominant pattern and shows various clinical features. The author's case was a 7-year old boy, who had been managed since birth for bilateral congenital resistant clubfeet including cast correction for six months and two operations. At present he represents not only the common features described above but also some unique features, e.g. pectus excavatum, posterior dislocation of both elbows, mild genu valgum, metatarsus adductus and complex malalignment of carpal and tarsal bones. To our knowledge, there is no report on Pfeiffer syndrome in Korea. The authors report a case of Pfeiffer syndrome with review of literatures.
Acrocephalosyndactylia ; Dislocations ; Elbow ; Funnel Chest ; Genu Valgum ; Head ; Humans ; Intelligence ; Korea ; Male ; Metatarsus ; Parturition ; Syndactyly ; Tarsal Bones ; Thumb ; Toes

One hundred and fifty two open wounds of extremities including open fractures were treated at the Kyoungpook Nstional Univ. Hospital from March 1977 to August 1977. Of 152 open wounds there were 32 cases (21.5%) of wound infection even though the initial wound culture revealed bacterial growth in 44 cases(28.9%). However, there were only 10 cases of infection among the cases with positive bacterial growth in the original culture and the same organisms were found in the 6 cases between the initial wound culture and post infection culture. Sensitivity studies of the organisms, the Cephalosqorines appeared to be most effective as a prophylactic antibiotics. It was sensitive to all gram positive organisms and majority of gram negative organisms including Escherichia coli. In bacterial culture and sensitivity studies of the 82 open fracture wounds 18 of them showed gram positive and gramnegative bacteria. The prominant organism were Staphylococcus, Escherichia coli, Streptococcus and Pseudomonas. The infection rate was lower in the cases whar the wound was managed within six hours from the time of injury than after six hours.
Anti-Bacterial Agents ; Bacteria ; Escherichia coli ; Extremities ; Fractures, Open ; Humans ; Pseudomonas ; Staphylococcus ; Streptococcus ; Wound Infection ; Wounds and Injuries

Gas producing infection accompanied by evidence of subcutaneous gas usually are diagnosed as clostridial gas gangrene. The occurrence of nonclostridial gas producing infection is thought to be relatively rare. Two cases of nonclostridial gas producing infection simulating clostridial gas gangrene in diabetes occurred. Gram stains of pus at the time of admission can isolate the E-coli and streptococcus, and can distinguish these two entities. Chief conplaints were swelling, pain and tenderness of right lower extremity by infection. Multiple incisions to free the tissues of gas and pus and the administration of antibiotics are the treatment of choice.
Anti-Bacterial Agents ; Coloring Agents ; Gas Gangrene ; Lower Extremity ; Streptococcus ; Suppuration

No abstract available.
Child* ; Humans ; Immunoglobulins ; Infant, Newborn* ; Lactoglobulins ; Milk Proteins* ; Milk* ; Mothers* ; Postpartum Period*

A multi-locus probe. called pHK-450. detecting multiple variable DNA fragments, was constructed by cloning of the 450 bp fragments into pCR(TM) vector, which could be obtained from amplification of the variable region of the D8S210 locus of human genome with a primer set of UF1. UR1, and UR2. The sequence data shows that the variable region is entirely G-C rich on one strand consisting of tetranucleotide repeat unit (GGAA)n at both margins and internally diversed regions with simple irregular repeat units of GGAA, GGAG, or GGGAA. The genetic complexixty of the resulting individual-specific DNA patterns was investigated by studying a Korean pedigree(both parents and 10 children). HaeIII and HinfI-generated DNA fragments are inherited to their children in a Mendelian manner with segregation ratio of 1:1.05 and 1:1.14. respectively. In the HinfI-digested genomic DNAs, the segregation of up to 25 variable DNA fragments from both parents could be analysed in a single sibship. Most of these variable DNA fragments detected by pHK-450 probe are not paired as allele, which suggests that they may be derived from 23 heterozygous loci. In the HaeIII-digested genomic DNA, 26 variable DNA fragments are segregated from both parents to 10 children. Excluding one allelic and three linked DNA fragments, 22 out of 26 scored DNA fragments may be derived from 23 hetrozygous loci. To determine the variability of DNA fragments, DNA samples from 113 unrelated Korean were digested with HinfI and hybridized using pHK-450 probe. Pairwise comparisons of individual variable DNA fragments show that the polymorphic patterns were highly specific to an individual. The mean probaility(X) that fragments in A individual is present in B individual is 0.0337. From the degree of maxiaml sharing, band approximate estimates of mean allele frequency and homozygosity is calculated being 0.187 and the mean heterozygossity is 89.69%. For pHK-450 probe, the probability that all 10 resolved DNA fragments in the 5-30 kb size range in an individual A are also present in a second unrelated individual B is (0.337)(10) or 7.1 X 10(-6).The above results suggest that the Muiti-locus probe, pHK-450, is suitable for rapid polymorphic marker generation and can be applied to individual identification in forensic medicine
Alleles ; Child ; Clone Cells ; Cloning, Organism ; Colon* ; DNA ; Forensic Medicine ; Gene Frequency ; Genome, Human ; Humans ; Microsatellite Repeats ; Parents

No abstract available.
Hyperhidrosis* ; Thoracotomy*

Cryptococcosis is a subacute or chronic mycotic infection caused by cryptococcus neoformans with a special predilection for the lung, skin and central nervous system, but rarely involving the lymphatic system. Recently the authors have experienced two patients with cryptococcosis in volving the liver and lymph nodes. One was 31/4year old male and the other was 11 year old male. The former had suffered from severe abdominal pain since about 1 month ago and high fever developed 10days before admission. On physical examination, jaundice and hepatomegaly were noted with enlarged and tender lymph nodes in the neck and inguinal region. Bone marrow aspiration revealed increased cellularity with eosinophilia. Lymph node and liver biopsy specimens showed mand granulomas including epithelioid cells and Langhan's type giant cells containing numerous cryptococci. The budding cryptococci were also found in the cerebrospinal fluid prepared with India ink. The latter patient was admitted with the chief complaint of high fever and neck mass which had developed one week ago. On physical examination, enlarged lymph nodes were noted in the neck. But hepatomegaly or splenomegaly was not found. Lymph node bisopsy specimen revealed the same findings with the former case. They were treated with Amphotericin B with some improvement, but as the wre discharged because of poor economic condition the were not sufficiently followed up.
Abdominal Pain ; Amphotericin B ; Biopsy ; Bone Marrow ; Central Nervous System ; Cerebrospinal Fluid ; Child* ; Cryptococcosis* ; Cryptococcus neoformans ; Eosinophilia ; Epithelioid Cells ; Fever ; Giant Cells ; Granuloma ; Hepatomegaly ; Humans ; India ; Ink ; Jaundice ; Liver ; Lung ; Lymph Nodes ; Lymphatic System ; Male ; Neck ; Physical Examination ; Skin ; Splenomegaly