No abstract available.
Ambulatory Care Facilities* ; Humans ; Outpatients*

No abstract available.
Animals ; Antibodies* ; Antibodies, Anticardiolipin ; beta 2-Glycoprotein I* ; Mice* ; T-Lymphocytes, Helper-Inducer*

No abstract available.
Brain Death* ; Brain*

BACKGROUND: Cyclic flow variations(CFVs) is defined as morphological evidence of wide flow velocity variations in the Doppler signals due rapid spontaneous changes showing cyclic reduction and abrupt reperfusion of blood flow velocity seen in the critically stenotic arteries. Since first development of the CFVs model using dog by Folts and Uchida, it has been widely used as exellent experiemental model for study of the Acute ischemic heart disease syndrome including unstable angina. Nowadays it has been well documented that these CFVs are closely associated with temporal platelet aggregation and followed thrombus formation at the stenotic arterial lesion with endothelial or medial injury and subsequent release of various chemical mediators, eg. thromboxan A2 and serotonin. Also the CFVs can be seen in some patients of coronary artery stenosis during underwent PTCA, femoral artery stenosis and carotid or cerebral artery stenosis as well as in animal models. Moreover, CFVs has been thought to be the natural preconditioning in the unstable angina. METHODS: We tried to make the CFVs model using left anterior descending coronary artery (LAD) in 6 dogs. Pericardial cradle was made through 5th intercostal thoracotomy. The LAD was isolated carefully and critically stenosed by plastic constrictor and Doppler velocimeter probe was placed under the constrictor. After then intimal and medial layer of the LAD was damaged by a forcep. After appearing of CFVs, we observed and recorded for an hour. Myeloperoxide(MPO) activity in the ischemic and non-ischemic area of the myocardium were studied and compared after sacrifice. RESULTS: CFVs was found in all 6 dogs within an hour. The mean frequency of the CFVs was 9.8+/-4.45 times/hour. The mean coronary blood flow was 5.7+/-2.7 ml/min. And MPO activity was 1.47+/-0.5 units/g tissue in the ischemic myocardium and 0.49+/-0.27 units/g tissue in the non-ischemic area with statistical significance(p<0.005). CONCLUSIONS: CFVs model using various animal models and arterial sites can widely provide usefulness to document pathophysiology and pharmacologic mechanism in human acute ischemic heart disease syndromes.
Angina, Unstable ; Animals ; Arteries ; Blood Flow Velocity ; Cerebral Arteries ; Constriction, Pathologic ; Coronary Stenosis ; Coronary Vessels* ; Dogs ; Femoral Artery ; Humans ; Models, Animal ; Models, Theoretical* ; Myocardial Ischemia* ; Myocardium ; Plastics ; Platelet Aggregation ; Reperfusion ; Serotonin ; Surgical Instruments ; Thoracotomy ; Thrombosis

The upper 8 centimeters of the great saphenous vein were studied on 102 sides (84male, 18 female) of adult Chinese cadavers. The chief results were as follows: 1. The surface projection of the sapheno-femoral junction is variable. It lies at avariable distance below the point which is situated opposite the junction of the lateraltwo thirds and medial third of the line joining the anterior superior iliac spine to thepubic tubercle. The mean value of the distance between this point and the sapheno-femoral junction is 3.84?0.07 cm (min. 2.1 cm, max. 5.5 cm). 2. Duplication of the great saphenous vein is rare. It is found on 6 sides only(5.88?2.33%) in our material. 3. There is a close relation between the terminal part of the great saphenous veinand the superficial external pudendal artery. In 65.63?4.85% of the cases, this arterypasses behind the terminal part of the vein. 4. The upper part of the great saphenous vein is joined by lateral, medial, or bothaccessory saphenous veins in 86 sides (84.31?3.60%), among which the lateral accessorysaphenous vein occurs most frequently (61.61?5.24%). 5. The junction between the deep external pudendal vein and the great saphenousvein lies within the fossa ovalies in 25 sides (24.51?4.26%). 6. The patterns of the tributaries of the great saphenous vein may be classifiedinto 4 types and 11 subtypes, according to the number of veins and the manner of theircombination. Type II_2, in which two tributaries are combined, occurs more often thanany other types (38.23?4.81%). In the point of view of practical application, the pat-terns of the tributaries may be classified into different types, according to the numberof the direct opening on the upper part of the great saphenous vein, among which the3 tributary and 4 tributary types are the highest in percentage, being 34.31?4.70% and33.33?4.67% respectively. 7. The position of the tributaries which open into the upper part of the greatsaphenous vein is variable. In the majority of sides (91.18?2.80%), the position lieswithin the uppermost 4 centimeters of the great saphenous vein (min. 0.3 cm, max.7.2 cm). The surgical significance is briefly discussed.

No abstract available.

This study was conducted to identify the factors related to the blood pressure in school children. The study subjects were 144 boys and 140 girls aged 10 years old, 4th grade student of one elementary school in Taegu City. Blood pressure and 18 variables for 284 school children were measured in May 1992. A weight-for-height index, Rohrer index(weightlheighe) was calculated for each individual as a measure of obesity. Body fat percent, fat weight, and lean body mass were measured by impedance fat meter(Model SIF-891). Serum total cholesterol, higli-density lipoprotein, triglyceride, uric acid, total protein, and electrolyte were measured by automated clinical chemistry analyzer(Hitachi 4020). Low-density lipoprotein and atherogenic index were calculated by the equation. The variables assessed were sex, systolic blood pressure, diastolic blood pressure, Rohrer index, body fat percent, body fat weight, lean body mass, total cholesterol, high-density lipoprotein, low-density lipoprotein, uric acid, total protein, serum electrolyte and blood glucose. The mean systolic and diastolic blood pressures for boys were 104.1mmHg and 66.6mmHg and those for girls were 102.9mmHg and 67.5mmHg. Systolic blood pressure of boys was positively correlated with height, weight, Rohrer index, fat weight, lean body mass, and triglyceride. Systolic blood pressure of girls was positively correlated with height, weight, lean body mass, and uric acid. Diastolic blood pressure of boys was positively correlated with weight, Rohrer index, and lean body mass. Diastolic blood pressure of girls was positively correlated with height, weight, fat weight, and lean body mass and negatively with total serum protein. In multiple regression analysis for the systolic blood pressure, the significant independent variables were Rohrer index and serum sodium in boys, and serum uric acid, fat percent, and Rohrer index in girls. In multiple regression analysis of the diastolic blood pressure, the significant independent variables were Rohrer index in boys and total serum protein in girls. This study indicated that important factors influencing blood pressure in school children were Rohrer index, fat percent, serum sodium, uric acid, and total protein. Rohrer index was most important among these. It is recommended to enforce the nutritional education for diet control of obesity to prevent hypertention in children and to adopt healthy life-styles that promote good health and prevent development of cardiovascular risk factors.
Adipose Tissue ; Blood Glucose ; Blood Pressure* ; Chemistry, Clinical ; Child* ; Cholesterol ; Daegu ; Diet ; Education ; Electric Impedance ; Female ; Humans ; Lipoproteins ; Obesity ; Risk Factors* ; Sodium ; Triglycerides ; Uric Acid

OBJECTIVES: To induce osteogenesis of cultured mesenchymal stem cells(MSCs) isolated from rat bone marrow in vitro and in vivo. METHODS: Bone marrow cells were isolated from Spraugue-Dawley rats and seeded in culture medium. MSCs were isolated from bone marrow cells and cultured. For in vitro induction of osteogenesis, MSCs were cultured in osteogenic medium. After 2 weeks, evaluation of osteogenesis was performed with alkaline phosphatase(ALP) activity, ALP immunochemical stain, von-Kossa stain for mineral deposition, and Northern blot analysis for osteocalcin m-RNA. For in vivo induction of osteogenesis, MSCs were seeded on Collagraft strips. The Collagraft strips seeded with MSCs were grafted in the subcutaneous tissue of the back of immunocompromised rats. Grafted Collagraft strips were harvested 3, 6, 9, 12 weeks after graft and stained with H-e ad toluidine blue-O stai. RESULTS: In vitro assay ALP activity was 121.27 p-nitrophenol nmol/106cell in osteogenesis induced MSCs and 75.53 p-nitrophenaol nmol/106cell in control groups. Osteogenesis induced MSCs were stongly stained by ALP immunochemical stain. Von Kossa stain showed black mineral deposition. There were strong bands in Northern blot analysis for osteocalcin m-RNA. H-E stain showed penetratin of cellular component into the pores of Collagraft strip in all specimen and showed formation of osteoid from 6 weeks after graft. Toluidine blue-O stain showed blue staining cartilagious component from 3 weeks after grafting. CONCLUSION: We could induce osteogenesis from mesenchymal stem cells in vitro and in vivo. Based on the results of this study, bone graft might be able to replaced by a new method in the near future.
Animals ; Blotting, Northern ; Bone Marrow Cells ; Bone Marrow* ; Mesenchymal Stromal Cells* ; Osteocalcin ; Osteogenesis* ; Rats* ; Subcutaneous Tissue ; Transplants

No abstract available.
Complement System Proteins* ; Humans ; Infant, Newborn*

No abstract available.