Objective To find a ideal method for inguinal herniorrhaphy. Methods One hundred patients with inguinal hernia were randomized to receive respectively PHS and Mesh Plug & Patch tension-free herniorrhaphy. Results In PHS group, the average operative time was 35 min. The postoperative complication rate was 2% (1/50), the average hospital stay was 2 days. Follow-up of 6～10 months has found no recurrence. In Plug group, the average operative time was 40 min,complication rate was 8% (4/50), the average hospital stay were 3 days. Postoperatively, 6 patients complained of temporary foreign body feeling. Follow-up of 6～10 months has found no recurrence in either group. Conclusions Both PHS and Mesh Plug & Patch are ideal materials for herniorrhaphy. PHS is especially suitable for lean inguinal hernia patients with large defect on transversalis fascia. Plug & Patch has an advantage in fat patients with small defect on transversalis fascia.

OBJECTIVETo detect mosaic trisomy 9 missed by conventional cytogenetics.

METHODSPeripheral blood genomic DNA from a girl with mental retardation was analyzed using Affymetrix CytoScan (TM) HD array. Fluorescence in situ hybridization (FISH) was also performed on samples from two patients.

RESULTSThe SNP-array analysis has revealed multiple duplications along chromosome 9. FISH analysis showed that, for the peripheral blood sample from one patient, 40 of 100 interphase cells and 15 of 100 metaphase cells carried trisomy 9. For the cord blood sample from another patient, 35 of 100 interphase cells and 10 of 100 cultured cells carried trisomy 9.

CONCLUSIONSNP-array is useful for detecting low-level mosaicism which may be missed by conventional cytogenetics. Combined with karyotype and microarray analyses, FISH is a focused and targeted approach for diagnosing mosaic trisomy. They may provide a useful tool for differentiating pseudomosaicisms from true mosaicisms.

Adult ; Chromosomes, Human, Pair 9 ; genetics ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Infant ; Male ; Mosaicism ; embryology ; Oligonucleotide Array Sequence Analysis ; instrumentation ; methods ; Polymorphism, Single Nucleotide ; Pregnancy ; Prenatal Diagnosis ; Trisomy ; diagnosis ; genetics ; Uniparental Disomy ; cytology ; diagnosis ; genetics