OBJECTIVE:To provide references for the understanding and implementation of the basic principles for clinical use of traditional Chinese medicine injections.METHODS:The main problems appeared in clinical use of traditional Chinese medicine injections and their effects on the medication safety of the injections were analyzed.RESULTS & CONCLUSION: Clinical rational use of traditional Chinese medicine injections is an important link in the process of ensuring the medication safety of the injections.The formulation of the basic principles of clinical use of traditional Chinese medicine injections is quite necessary aimed at the problems occurred in the clinical use of traditional Chinese medicine injections,and the principle serves as a guidance for rational use of traditional Chinese medicine injections.

Objective: To establish the determination method for meropenem in urine by fluorescence resonance energy transfer spectroscopy. Methods:An LS-55 fluorescence spectrophotometer was used. The solution of meropenem, fluorescein, eosin Y, BR buffer and cetyl trimethyl ammonium bromide was respectively added into a 10 ml colorimetric tube. At λex of 455 nm and λem of 547 nm, the fluorescence intensity F and F0 of the system and the reagent blank sample was detected. Using ΔF = F-F0 as signal re-sponse value for meropenem, the content was calculated. Results: The linear regression equation of meropenem in urine was as fol-lows:ΔF=33. 8C+53. 4 (r=0. 991 7), the linear range was 0. 5-10 μg·ml-1 with the detection limit of 0. 13 μg·ml-1, the re-covery of 98. 9%-103. 0% and RSD of 0. 3%-0. 4%. Conclusion:The method is rapid and accurate, which can be used in the phar-macokinetic study of meropenem.

OBJECTIVE: To investigate the inhibitory effect of hydroxycamptothecin (HCPT) on C6 gliomas cells in rats. METHODS: The cytotoxicity of HCPT at different concentration(1～0.031 25 mg?mL-1) on C6 in vitro was measured by MTT with conventional therapy for C6 gliomas cells as control. Meanwhile,the antitumor effect of O-HCPT(open ring form) and that of C-HCPT(closed ring form) were compared. RESULTS: At a high concentration,HCPT showed an inhibition ratio of as high as(69.43?4.5)% on C6 gliomas cells in concentration-dependent manner,showing no significant differences as compared with control. The antitumor effect of O-HCPT was lower than that of C-HCPT,and their IC50 were 3.626 mg?mL-1 and 0.135 mg?mL-1,respectively. CONCLUSION: HCPT had remarkable inhibitory effect on C6,and the antitumor effect of C-HCPT is potent than that of O-HCPT.

OBJECTIVE:To prepare hydroxycamptothecin sustained-release tablets and to optimize its preparation technology and formulation.METHODS:The preparation technology and formulation were optimized by orthogonal experiment taking the type of excipient polylactic acid (A),tabletting pressure (B),ratio of HCPT to PLA (C) as factors with the deviation degree of the accumulative release percentage as evaluation index.Then the optimized results were verified.RESULTS:The optimal technology and formulation were as follows:A was PLA (20 000),B was 250 N,C was 1∶5.The deviation degree of the formulation was verified to be 2.963.CONCLUSION:The hydroxycamptothecin sustained-release tablets prepared in optimized preparation technology and formulation are up to the standard on preparation.

OBJECTIVE:To survey and objectively evaluate the present situation and trend of the usages of the antivirals METHODS:To make a survey of the usage for above-mentioned drugs in 136 hospitals of 6 cities in the Yangtse valley of China during the period 1998～2000 concerning the sum of money for drugs,main pharmaceutical enterprises etc and to analyse the situation of clinical use and trend forecast RESULTS:The sum of money for the antivirals was 17 330 331 yuans,24 068 239 yuans and 47 427 419 yuans in 1998,in 1999 and in 2000,respectively Total expenditure and consumption for the antivirals in China increased year by year CONCLUSION:The antivirals is a kind of drug which has developmental potential,the expenditure and consumption will increase rapidly

OBJECTIVE:A HPCE method for determination of paeoniflorin in Bazhen pills and Bazhen Yimu pills was developed METHODS:Measurements were carried out in a buffer solution containing 50mmol/L borax solution (pH 10 6),at detection wavelength 230nm with 20KV running voltage applied to a 50cm?50?m capillary RESULTS:The linear range of paeoniflorin was in the range of 0 0 625～1 0mg/ml(r=0 9 995),RSD within days was 1 61%,RSD between days was 3 15% The recovery of paeoniflorin was 98 19%～98 45% The results of detection for Bazhen pills and Bazhen Yimu pills were highly correlated to those obtained by HPLC CONCLUSION:The method is simple,rapid,economical and accurate

Objective To investigate the dissolution rates of glibenclamide and metformin in compound metformin hydrochloride. Methods The concentrations of the glibenclamide and metformin were determined by HPLC method. The rotating basket method was used to measure and compare the dissolution rates of glibenclamide and metformin between the compound metformin hydrochloride and conventional tablets. Results The dissolution rates of glibenclamide and metformin with different preparation came up to the standard of CHP (2005), but the dissolution parameters were different. Conclusion The dissolution rate of glibenclamide indicated significant difference between the compound metformin hydrochloride and the conventional tablets, and no significant difference for metformin.

OBJECTIVE:To evaluate the curative efficacy of Chinese medicine vs. western medicine in the treatment of diabetes. METHODS:The randomized controlled trials(RCTs) comparing western medicine with traditional Chinese medicine alone or combination of Chinese medicine and western medicine in the treatment of diabetes were systematically evaluated. Meta-analysis of the cited trials was performed by evidence-based medicine method with Revman 4.2 software for statistical analysis. RESULTS:A total of 14 RCTs met our inclusion criteria. Meta-analysis showed that there was no significant difference between traditional Chinese medicine and western medicine in clinical efficacy in the treatment of diabetes; however,the diabetic patients treated with integrated Chinese medicine and western medicine showed much better efficacy than those treated with western medicine alone. CONCLUSION:Treatment of diabetes with traditional Chinese medicine plus western medicine has more advantages; however,this conclusion remains to be confirmed further by studies of high quality given the low quality in test methodology.

OBJECTIVE:To study the safety of polylatic acid used as sustained-release drug carrier in brain.METHODS:The toxicity and apoptosis of in vitro primary cultured cortical neurons in rats were evaluated using CCK-8 and Hoechst33258 staining after treated with polylatic acid and compared with the normal control group.Self-made polylatic acid blank tablet was implanted into rat brain and after 30 days the neuronal function was assessed through behavior ability testing,HE staining and Nissl staining,compared with sham groups and normal control group.RESULTS:When neurons were cultured for 7,9 and 12 days in polylatic acid group,there was no significant difference in absorbance value of CCK-8 and apoptosis rate between normal control group and polylatic acid group.Light inflammatory was observed in rat brain tissue in polylatic acid blank tablet group after 30 days,as compared with sham group.But the morphology,number and function of neuron had no obvious change.CONCLUSION:Polylactic acid has no significant inhibitory and inducing apoptosis effect on in vitro cultured cortical neurons and also has no obvious toxicity on brain tissue.

OBJECTIVE:A HPCE method for determination of Quercetin in acanthopanax and acanthopanax injection was developed.METHODS:Measurements were carried out in a buffer solution containing 50mmol/L boric acid solution and 50mmol/L SDS(pH 8.5),at 20kV running voltage applied to a 50cm?50?m capillary.The detection wavelength was 260nm.RESULTS:The linear range of quercetin was 0.0 125～1.0mg/ml.The recovery of acanthopanax was (98.99?0.63)%.The recovery of acanthopanax injection was (98.62?0.42)%.CONCLUSION:The method is simple,rapid,accurate and suitable for determination of quercetin in acanthopanax and acanthopanax injection.