1.Study on the relationship between infiltration and transfer of bladder carcinoma and the fibrin level
Journal of Jilin University(Medicine Edition) 2000;26(6):610-611
Objective:To explore the mechanism of infiltration and transfer of bladder carcinoma. Meth-ods:Ager single diffusion method and immunoenzyme technique were adopted to determine the level anddistribution of fibrin in serum and tissues of bladder carcinoma. Results :Fibrin level in patients with blad-der carcinoma significantly decreased than that in control (P<0. 01). In tissues of bladder carcinoma,mostpart of FN was expressed in cellular matrix,and a little FN was on the surface of cell membrane. Positiveexpression in transfer bladder carcinoma significantly decreased than that in non-transfer bladder carcino-ma. Conclusion :FN level relates to the infiltration and transfer in bladder carcinoma patients.
2.Autophagy and tumors
Journal of International Oncology 2013;(5):325-328
Recent studies show that autophagy ont only plays an important role in maintaining homeostasis in cells,but also palys a double role in the tumorigenesis and development of cancer.Studying the molecular mechanisms of autophagy and the relationship between autophagy and cancer have great significance for cancer treatment and prevention.
3.Determination of Meropenem in Urine by Fluorescence Resonance Energy Transfer Spectroscopy
China Pharmacist 2014;(12):2016-2018
Objective: To establish the determination method for meropenem in urine by fluorescence resonance energy transfer spectroscopy. Methods:An LS-55 fluorescence spectrophotometer was used. The solution of meropenem, fluorescein, eosin Y, BR buffer and cetyl trimethyl ammonium bromide was respectively added into a 10 ml colorimetric tube. At λex of 455 nm and λem of 547 nm, the fluorescence intensity F and F0 of the system and the reagent blank sample was detected. Using ΔF = F-F0 as signal re-sponse value for meropenem, the content was calculated. Results: The linear regression equation of meropenem in urine was as fol-lows:ΔF=33. 8C+53. 4 (r=0. 991 7), the linear range was 0. 5-10 μg·ml-1 with the detection limit of 0. 13 μg·ml-1, the re-covery of 98. 9%-103. 0% and RSD of 0. 3%-0. 4%. Conclusion:The method is rapid and accurate, which can be used in the phar-macokinetic study of meropenem.
4.Clinical Analysis of 102 Cases of ARDs Induced by Angiotension Converting Enzyme Inhibitors
Yongxin LI ; Zhiyuan SONG ; Songqing LIU
China Pharmacy 2001;12(5):298-299
OBJECTIVE: To analysis the adverse reactions of angiotension converting enzyme inhibitor( ACEI) , so as to deepen the understanding to the ARDs and improve the therapeutic effect of ACEI METHODS: The therapeutic effects and ARDs of ACEI were retrospectively reviewed and the incidence of ARDs induced by ACEI and the relationship between different ACEIs and their ARDs were analyzed RESULTS: ( 1) The incidence of ARDs of ACEI was 12% Stimulating cough, parageusia, gastrointestinal tract discomfort, skin rash, itch of skin, hypotension, headache, dizziness, proteinuria and angioedema were the common symptoms ( 2) Of five kinds of ACEIs, captopril induced the highest incidence of ARDs and stimulating cough, parageusia, gastrointestinal tract discomfort, skin rash and itch of skin were the most common manifestations CONCLUSION: Stimulating cough, parageusia, gastrointestinal tract discomfort and skin rash are the most common symptoms of ARDs, and the symptoms will disappear when drug administration is discontinued
5.Anti-apoptosis effect of ligustrazine on the apoptosis of PC12 cells induced by dopamine
Zhigang LI ; Lei ZANG ; Songqing LIU ;
Journal of Third Military Medical University 2003;0(18):-
Objective To explore the protective effect of ligustrazine on the apoptosis of PC12 cells induced by dopamine(DA) and its mechanisms. Methods Apoptosis of PC12 cell was induced by dopamine at the concentrations of 0, 0.30, 0.60 mM. Ligustrazine at the concentrations of 0, 30, 60, 90 ?g/ml was added to defect the effect of ligustrazine on apotosis. The subdiploid peaks showing cell apoptosis rate were detected by flow cytometry. The cell activity was determined by MTT. Concentration of nitric oxide(NO) was determined by the Griess reaction. Results Compared with those of the control, the apoptosis rate and the level of NO induced by 0.30 or 0.60 mmol/L DA were higher, but the activity of PC12 cells was lower. Compared with those of the groups without ligustrazine, the apoptosis rate and the level of NO increased, but the cell activity decreased in a dose dependent manner. Conclusion Ligustrazine can inhibit the apoptosis induced by DA, which might be correlated with the decrease of NO production.
6.Effect of Xingnaojing Injection on PMN Adhering to BMVEC in Vitro
Zhigang LI ; Songqing LIU ; Zhangyang WANG
China Pharmacy 1991;0(06):-
OBJECTIVE:To investigate the anti-adherence effect and its mechanism of Xingnaojing(XNJ)injec?tion.METHODS:In mixed culture of rabbit’s PMN and brain microvascular endothelial cell(BMVEC),the effects of XNJ on LPS-induced PMN-BMVEC adhesion and the changes of PAF and NO levels were observed.RESULTS:XNJ could obviously decrease the PMN-BMVEC adhesion rate and the levels of NO and PAF in culture medium.CONCLUSION:XNJ can inhibit adhesion of PMN to BMVEC and release of some inflammatory media.The decrease of some inflammatory media may related to the reduction of adhesion.
7.Neck Acupuncture is Used to Treat 95 Cases with Acute Cerebral Embolism
Songqing CHEN ; Yiping WANG ; Yixin LI
Chinese Journal of Rehabilitation Theory and Practice 1997;3(4):161-163
In this article routine treatment and neck acupuncture were used in 95 cases with acutecerebral embolism. The results show that the evaluation of mark with Neurological Function Distress is remarkedly reduced,and cure rate and general effective rate are more higher than routine treatment (contrast,P<0.01) .This study suggests that neck acupuncture is a simple and effective theapy of cerebral embolism.
8.Expression of TRAIL and TRAILR in human hepatocellular carcinoma and its therapeutic significance
Zhenyu XIAO ; Xiaoping CHEN ; Songqing HE ; Zhiyong HUANG ; Li LI
Chinese Journal of General Surgery 2001;0(08):-
Objective To evaluate the expression of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) and its receptor(TRAILR) in human hepatocellular carcinoma (HCC), and its therapeutic potential for human HCC. Methods Expressions of TRAIL and TRAILR were determined in and adjacent to carcinoma tissues, and normal liver tissues. The cytotoxic effects of TRAIL in combination with chemotherapeutic agents on HCC cell lines were detected. Results Expression of TRAIL in tumor adjacent tissue was higher than in cancer tissue. There were high expression of DR and low expression of DcR in HCC tissue in contrast to normal hepatic tissue(?~ 2=4.68,P
9.To compare the role of Gd-EOB-DTPA dynamic contrast-enhanced MRI with multi-detector row computed tomography (MDCT) in patients with focal liver lesions
Xiaoqing LI ; Zhipeng ZHOU ; Songqing HE ; Xiaoping CHEN
Chinese Journal of Hepatobiliary Surgery 2013;19(11):815-820
Objective To compare the role of Gd-EOB-DTPA dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with multi-detector row computed tomography (MDCT),and to determine the sensitivity,specificity and accuracy in focal hepatic lesions.Methods A retrospective analysis was conducted on 32 patients with focal hepatic lesions who had undergone MRI and MDCT examinations.These patients were divided into two groups:the CT group and the MRI group.The results were analysed using receiver operating characteristic (ROC) curves.Result There were 185 focal hepatic lesions.The sensitivity,specificity and the area under the ROC curve (AUC) were 86.5%,90.9%,0.855,respectively for the MRI group and they were significantly higher than the CT group (63.6%,54.5%,0.532).For detection of lesions <1 cm,the sensitivity,specificity and the area under the ROC curve (AUC) for the MRI group were 90%,86.6%,0.886,respectively,which were also significantly higher than the CT group (50.5%,45.5%,0.500).When combined with pathological findings and follow-up,the diagnostic accuracy was 40.6% using Gd-EOB-DTPA DCE-MRI.Conclusion Gd-EOB-DTPA DCE-MRI has a higher detection rate,better accuracy and diagnostic value for focal liver lesions (<1 cm) than MDCT.
10.Determination of the Content of Ceftazidime in Renal Dialysate Effluent in Burn Patients
Cai LIN ; Songqing LIU ; Qing DAI ; Hongbin LI ; Yizhi PENG
China Pharmacy 2001;0(10):-
OBJECTIVE:To determine the content of ceftazidime in renal dialysate effluent in burn patients.METHODS:HPLC was used.The sample was separated on Bondapak-C18 analysis column with a mobile phase of mixture of methanol-ammonium acetate(17∶83,V/V).The flow rate was 1.0ml/min.The UV detector wavelength was set at 254nm.RESULTS:A linearity was obtained from 0.5~100?g/ml with a good correlation(r=0.9 999).The RSDs of inter-day and intra-day precision were not more than 3.The average recovery was 100.30%.CONCLUSION:The method is simple,rapid and accurate.