Purpose To study the clinicopathologic features of juxtaglomerular cell tumor ( JGCT) . Methods The microscopic and immunohistochemical features were examined in 5 cases of JGCT, with review of related literatures. Results Four patients were fe-males and 1 was male, average aged 39 years ( range, 26-60 years) . Four patients had hypertension at diagnosis. Postoperative serum renin and aldosterone levels decreased obviously than before the operation in one patient. In one patient tumor had metastasized to liver and bone and died 14 months after operation. Microscopically, the tumors were well circumscribed and at least partially invested by a thick fibrous capsule. Entrapped tubules were identified. Numerous hemangiopericytoma-like vessels were focally present. The tumor tissue was made up of sheets of ovoid to polygonal cells with regular nuclei, distinct cell borders, moderately abundant eosinophilic cy-toplasm. Mast cells and lymphocyte were common. Thick-walled hyalinized blood vessels were obvious. Necrosis and high mitotic ac-tivity were identified in one case. Immunohistochemically, tumor cells were positive for renin, CD34, vimentin, and SMA (focally). Conclusions JGCT is a rare renal neoplasm. Its diagnosis and differential diagnosis must be established on the clinicopathologic fea-tures, immunohistochemical findings and ultrastructural features. Malignant JGCT is very rare. Its criteria are unknown, but malignant JGCT include histological vascular invasion, a large tumor size, relatively advanced patient age or child, necrosis and/or high mitotic activity.

Objective To study the expressions of mucin (MUC) 1 and 2 in extramammary Paget's disease(EMPD) lesions. Methods Tissue specimens were obtained from the lesions of 19 patients with EMPD and normal skin of 19 human controls during cosmetic surgery. Streptavidin-perosidase (SP) technique was used to detect the expressions of MUC1 and MUC2 in these specimens. Results As haematoxylin-eosin (HE) staining showed, 3 cases were accompanied by poorly differentiated adenocarcinoma, 6 were invasive Paget's disease and 10 were intraepithelial EMPD. MUC1 was expressed in 2 cases accompanied by poorly differentiated adenocarcinoma, and in all the cases of invasive and intraepithelial EMPD; MUC2 was observed in all the cases of adenocarcinoma-complicated EMPD and invasive EMPD, but only in 2 of 10 cases of intraepithelial EMPD.Neither MUC1 nor MUC2 was observed in normal control specimens. A significant increase was observed in the expression of MUC1 in lesions of intraepithelial EMPD compared with invasive EMPD and adenocarcinoma-complicated EMPD (both P ＜ 0.05), and in the expression of MUC2 in lesions of invasive EMPD and adenocarcinoma-complicated EMPD compared with intraepithelial EMPD (both P ＜ 0.05). The expression of MUC1 was uncorrelated to that of MUC2 (r= -0.5, P＞ 0.05). Conclusions MUC1 is generally expressed in the lesions of EMPD, while MUC2 is expressed in those of adenocarcinoma-complicated EMPD and invasive EMPD.

Objective To Evaluate the environment quality in underground construction site based on the measurement of atmosphere composition and microclimate. Method Concentrations and composition of gases were analyzed by combined detection with mass spectrometer and gas chromatograph. Microbial content was detected by striking method. A microclimate model was built based on the measurements of temperature, humidity and wind speed in two years. Result Concentrations of inorganic gas was higher than national standard. Total volatile organic compounds were in the range of 4～6 mg/m3, which highly exceeded the national standard. Concentration of O2 descended obviously at the time when personnel went into the construction site, and was lower than the national standard of 299 mg/m3 after continuous working for 3 h. When an aerator was started, the average noise level was in the range of 70～93 dB, which was higher than the national standard(55～70 dB). In the course of working, the average microbial content reached 4 000 cfu/m3 which was 3 times as high as the background level. The microclimate characteristic with high humidity, low temperature and inadequate sunshine was unsuitable for dwelling. Conclusion Environment in the underground construction site is unfavorable as compared with that on the ground. The physiological function of working staff may be influenced obviously due to cumulative effect of the above mentioned factors, and may lead to aprosexia, hypomnesis, fatigue, etc. Six kinds of protection means are suggested in the paper.

Objective To study the effects about using separated bed transfer to handle orthopedic patients.Methods According to the characteristics of carrying orthopedic patients,separated bed transfer was developed and made followed by trials in trial departments.A questionnaire survey of 214 patients and 27 users was completed and the related situation using separated bed transfer to handle orthopedic patients was surveyed,the results were compared with those of dead lift.Results Compared with traditional dead lift,there was obvious difference in the number of handling participators[≥5 persons:6.07％ (13/214) vs.21.96％(47/214);4 persons:35.51％ (76/214) vs.48.13％(103/214)],patients' comfort during handling [28.97％ (62/214) vs.84.11％(180/214)],x2=43.027,154.381,all P ＜ 0.05.Conclusions It can reduce the number of participators and patients' pain when handling patients using separated bed transfer.It is widely used with lower cost,which is worth being promoted in clinic.

Objective The antioxidative activities of extracts from Undaria pinnatifida in vitrowere tested. Methods Using the assay system of peroxide value (POV), diphenyl picryl-hydrazyl (DPPH), the antioxidative activities of various extracts were studied and comparedwith VE and VC. Results Different extracts from Undaria pinnatifida showed antioxidativeactivities, and petroleum ether extract showed the highest free radical scavenging efficiency.Conclusion petroleum ether extract has stronger antioxidative effect than others.

Objective Bioactive components were extracted from Sargassum fusiforme by using different solvents and their activities were detected in vitro in order to screen potential free radical scavenger.Methods Using the assay system of DPPH,superoxide radical,and in comparison with vitamin C,tea polyphenols and a series of extracts of sea tangle,laver.Results Different solvents had different extraction rates,and extracts by different solvents had different free radical scavenging activities.Conclusion 0.5%Na2CO3 solution had the highest extraction rate and extracts by this solution had the best scavenging ability as well.At the same time,the results obtained by DPPH detection method were more stable than those by the others.

In this paper,we summarize the teaching ideas,methods,and means for the course "Theory and Experiment of Molecular Biology" in which we teach students in an architecture-network-application way,exploring the education reform for the basic medical science.

This paper presents how to practically cultivate students' creative ability,scientific thinking and exploration ability in the molecular biology experiment course.

Objective To study the radioprotective effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B(UVB)-irradiated murine thmocytes in vitro. Methods The murine thymocytes were exposed to UVB radiation. MTT method was used to detect the cell viability. The activities of intracellular glutathione peroxidase (GSH-Px), superoxided dismutase(SOD) and catalase (CAT) were measured. Intracellular reactive oxygen species (ROS) and the effect of PCF on UVB-induced apoptosis were investigated by flow cytometry. Results PCF could greatly enhance the viability of murine thymocyte and markedly promote the activities of GSH-Px, SOD and CAT, while the amount of ROS was decreased. PCF could inhibit UVB-induced thymocyte apoptosis. Conclusion PCF has significant radioprotective effects on UVB-irradiated murine thymocytes in vitro.

Objective To investigate the antioxidative effect of polypeptide from Chlamys farreri (PCF) on HaCaT cells damaged by ultraviolet B (UVB). Methods HaCaT cells model established by UVB irradiation were randomly divided into seven groups. After pretreatment with different concentrations of PCF for 1 h, the cells were irradiated by UVB at a dose of 30mJ/cm~ 2, and followed further incubatiou for 18 h. Enzyme activities including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were determined by biochemical methods. Total antioxidative capacity (T-AOC) and malondialdehyde (MDA) were also determined. Results PCF could enhance the activities of SOD, GSH-Px and CAT; increased T-AOC and inhibited MDA formation. Conclusion These results indicated that PCF exhibited protective effects on HaCaT cells irradiated by UVB owing to its antioxidative action.