Objective To investigate the influence of RNA-interfered ribonucleotide reductase M2(RRM2)on apoptosis and invasion of ovarian cancer cells with drug resistant.Methods The specific small interfering(siRNA)transfected SKOV3/DDP of RRM2 gene were designed as the interference group.SKOV3/DDP cells,SKOV3/DDP-RRM2 non-specific negative cells were designed as the blank group and negative group.The cell proliferation inhibition rate was detected,RI and drug resistant transfection rate were calculated,the expression of RRM2 mRNA was detected by fluorescence PCR.The effect of siRNA transfection on SKOV3/DDP resistance index and RRM2 protein was analyzed,and the invasion ability of SKOV3/DDP cells was observed with Transwell.Results The transfection rate of the blank group,negative group and interference group was 90％.The drug resistance of DDPto SKOV3/DDP cells was low and gemcitabine was sensitive to SKOV3/DDP cells.DDP and gemcitabine of the DDP on cell half inhibitory concentration(IC50)values showed significant differences among three groups(P<0.05).IC50 values of DDP and gemcitabine on cells in the interference group were significantly lower than the negative group and blank group(P<0.05).The relative expression of SKOV3/DDP cells and SKOV3 cells RRM2 protein showed significantdifferences(P<0.05),and the relative expression was significantly lower in transfection group I,transfection group II and transfection group III than the negative group and blank group(P<0.05).The decrease of relative expression of RRM2 protein was the greatest in the transfection group I(P<0.05).The apoptosis rate was significantly higher in the interference group than the blank group and the negative group,transmembrane cells were fewer than the blank group and the negative group(P<0.05).Conclusion siRNA can effectively inhibit the proliferation and invasion of RRM2 gene in ovarian cancer.It can increase the drug sensitivity of drug resistant cells,especially cells promoting the apoptosis of drug resistant cells induced by DDP.

Objective To investigate the influence of RNA-interfered ribonucleotide reductase M2(RRM2)on apoptosis and invasion of ovarian cancer cells with drug resistant.Methods The specific small interfering(siRNA)transfected SKOV3/DDP of RRM2 gene were designed as the interference group.SKOV3/DDP cells,SKOV3/DDP-RRM2 non-specific negative cells were designed as the blank group and negative group.The cell proliferation inhibition rate was detected,RI and drug resistant transfection rate were calculated,the expression of RRM2 mRNA was detected by fluorescence PCR.The effect of siRNA transfection on SKOV3/DDP resistance index and RRM2 protein was analyzed,and the invasion ability of SKOV3/DDP cells was observed with Transwell.Results The transfection rate of the blank group,negative group and interference group was 90％.The drug resistance of DDPto SKOV3/DDP cells was low and gemcitabine was sensitive to SKOV3/DDP cells.DDP and gemcitabine of the DDP on cell half inhibitory concentration(IC50)values showed significant differences among three groups(P<0.05).IC50 values of DDP and gemcitabine on cells in the interference group were significantly lower than the negative group and blank group(P<0.05).The relative expression of SKOV3/DDP cells and SKOV3 cells RRM2 protein showed significantdifferences(P<0.05),and the relative expression was significantly lower in transfection group I,transfection group II and transfection group III than the negative group and blank group(P<0.05).The decrease of relative expression of RRM2 protein was the greatest in the transfection group I(P<0.05).The apoptosis rate was significantly higher in the interference group than the blank group and the negative group,transmembrane cells were fewer than the blank group and the negative group(P<0.05).Conclusion siRNA can effectively inhibit the proliferation and invasion of RRM2 gene in ovarian cancer.It can increase the drug sensitivity of drug resistant cells,especially cells promoting the apoptosis of drug resistant cells induced by DDP.

Cancer, as a persistent negative emotional stress source, brings serious harm to patients' body and mind, and directly affects their quality of life, treatment effect and disease outcomes. Scholars at home and abroad are actively exploring effective intervention methods to improve the negative emotions of cancer patients, and have achieved some results, but negative emotions of cancer patients are still widespread. This article reviews the expression and intervention of negative emotions in cancer patients,with a view to providing ideas and reference for the management of negative emotions in cancer patients.

Objective To explore the effect of peer education on the practical teaching of fundamental nursing for male nursing students .Methods Thirty-six male nursing undergraduate students from Grade 2011 were chosen as the observation group , and received the peer education .Thirty-two male nursing undergraduate students from Grade 2010 were chosen as the control group , and received the traditional teaching .The teaching effect was evaluated by the score of final examination and questionnaire in two groups .Results The scores of theory and practice test were respectively (57.68 ±4.34), (26.34 ±3.11) in the observation group, and were higher than (54.34 ±4.15), (24.58 ±2.93) in the control group, and the differences were statistically significant (t=3.233,2.393, respectively; P <0.05).The result of questionnaire on the teaching effect showed that the professional identification , learning interest , self-confidence , communications and collaboration abilities in the observation group were better than those of the control group (P<0.05), and no differences were found in the abilities of operation, problem analysis and innovation between two groups (P >0.05). Conclusions Peer education can improve the professional identification in the male nursing students , and increase their learning interest and self-confidence , and improve their scores of theory and practice test in the fundamental nursing , and increase their abilities of communications and collaboration .

Hip fractures are among the most common fractures in the elderly, presenting to be a leading cause of disability and mortality. Surgical treatment is currently the main treatment method for hip fractures. The incidence of perioperative malnutrition is increased after hip fractures in the elderly due to the comorbidities, decreased basal metabolic rate, accelerated protein breakdown, weakened anabolism and surgical stress. However, malnutrition not only increases the incidence of postoperative complications, but also leads to increased mortality, indicating an important role of perioperative nursing management of nutrition for the elderly patients with hip fractures. At present, there still lacks scientific guidance and application standards on perioperative nursing management of nutrition for the elderly patients with hip fractures. Therefore, the Orthopedic Nursing Committee of Chinese Nursing Association and the Editorial Board of Chinese Journal of Trauma organized relevant experts to formulate the Expert consensus on perioperative nursing management of nutrition for elderly patients with hip fractures ( version 2023) according to evidence-based medical evidences and their clinical experiences. Fourteen recommendations were made from aspects of nutrition screening, nutrition assessment, nutrition diagnosis, nutrition intervention and nutrition monitoring to provide guidance for perioperative nursing management of nutrition in elderly patients with hip fractures.