OBJECTIVETo establish an HPLC method to simultaneously determine six ingredients in zedoary turmeric oil and its related injections.

METHODHPLC analysis was performed on Waters Symmetry C18 (4.6 mm x 250 mm, 5 microm). The mobile phase was composed of methanol and water solution in gradient elution mode with at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 215 nm.

RESULTThe six ingredients were separated well. The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and beta-elemene were 1.74-347.00, 2.38-475.00, 2.49-497.00, 2.07-826.00, 5.05-1009.00, 4.78-955.00 mg x L(-1) (r > or = 0.9996), respectively. The average recoveries were above 95% (RSD <3.0%, n=9).

CONCLUSIONThe method is accurate, reliable and reproducible, it can be used for the quality control of zedoary turmeric oil and its related injections.

Chromatography, High Pressure Liquid ; methods ; Curcuma ; chemistry ; Injections ; Oils, Volatile ; analysis