1.THE CHANGES OF CALCIUM METABOLISM IN DIABETES MELLITUS AND ITS CLINICAL SIGNIFICANCE
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Six parameters of calcium metabolism - ICa, TCa, ICa / TCa, AKP, IntraCa (intracellular calcium), and UCa (urinary calcium) in 91 cases with diabetes mellitus (DM) were measured and compared with those in normal subjects. It could be seen that significant differences existed between the two groups in AKP, IntraCa and UCa (P
2.Pancreatic ?-cell dysfunction and type 2 diabetes
Chinese Journal of Endocrinology and Metabolism 1986;0(03):-
Both ?-cell dysfunction and insulin resistance contribute to the development of type 2 diabetes, and ?-cell function, especially early insulin secretion, is important in maintaining normal glucose tolerance. This review focuses on the ciritical role of ?-cell dysfunction in the pathogenesis of type 2 diabetes.
3.Pachydermoperiostosis
Qing LI ; Songhua WU ; Weiping JIA
Chinese Journal of Endocrinology and Metabolism 2008;24(4):461-462
Pachydermoperiostosis is a rare genetic disease affecting both skin and bones. Excluding some secondary causes such as pulmonary or cardiac disease, young men with digital clubbing, pachydermia and periostosis should be considered to be suffering from pachydermoperiostosis.
4.The value of cystatin C,homocysteine and super-sensitivity C-reactive protein in the atherosclerosis
Gonghe ZHANG ; Jierong WU ; Meng ZHOU ; Songhua WU ; Jinchao YANG ;
International Journal of Laboratory Medicine 2016;37(20):2818-2819,2822
Objective To investigate the clinical value of homocysteine (Hcy) ,cystatin C (Cys C) and high‐sensitivity C‐reactive protein (hs‐CRP) in atherosclerosis (AS) .Methods Totally 100 cases of newly diagnosed patients of AS were choosed as the ob‐servation group ,36 cases condition improved markedly of AS after treatment as the treatment group ,and 120 cases of normal medi‐cal groups as the control group ,the Hcy ,Cys C and hs‐CRP in all subjects were detected respectively .Results Hcy ,Cys C and hs‐CRP levels of observation group were significantly higher than those of normal groups ,and the difference was statistically signifi‐cant (P< 0 .05) .While the Cys C and hs‐CRP levels of treatment group were significantly lower than the those of observation group ,the difference was also statistically significant (P< 0 .05) .Conclusion The detection of Serum Hcy ,Cys C and hs‐CRP might be help to the diagnosis ,treatment evaluation and prognosis of AS .
5.Characteristic analysis of mitochondrial diabetes of Chinese with nervous deafness
Huijuan LU ; Xin LIN ; Songhua WU ; Guodong ZHANG ; Weiping JIA
Chinese Journal of Tissue Engineering Research 2001;5(21):147-148
Objective To detect mitochondrial gene 3243 A to 1507 Chinese and analyze their clinical characteristics.Methods Classical PCR- RFLP was used to detect the mutation and statistical analysis was performed on the data after stratification in accordance with presence or absence of diabetes.Results (1)In this study,prevalence of mitochondrial gene 3243 A to G mutation was 1.16~ 1.20% .(2)Compared with MDM(mitochondrial diabetes),M- NGT(normal glucose to lerance with mutation)showed no significant difference in body fat parameters,blood lipid and C- peptide except for early onset and hearing loss(P=0.0028).(3)Compared with type 2 DM,MDM presented characteristics of early onset,weight loss,hearing loss,islet cell function decrease and maternal hereditary.Conclusion Follow- up should be given to the carrying the mitochondrial gene 3243 A to G mutation but presenting generally normal clinical manifestation in order to give the patient in- time diagnosis and treatment.
6.Three kinds of internal fixations for treatment of complex tibial plateau fractures: a comparative biomechanical study
Songhua WANG ; Fan LIU ; Dengsheng LIU ; Hongfu WU ; Yijin WANG
Chinese Journal of Trauma 2009;25(9):829-833
Objective To evaluate the biomechanism of three kinds of internal fixations in treatment of complex tibial plateau fractures. Methods Eighteen human antiseptic cadaver tibial plateau specimens were used to make models of complex tibial plateau fracture (type Ⅵ fractures of Schatzker classification). The models were fixated with a lateral Golf-buttress plate (GP), modified dual plate (a lateral Golf-buttress plate plus a medial five-hole one-third tubular antiglide plate) (DP) or a lateral locking compression plate (LCP) respectively to compare strength, rigidity and stability of different fixation methods. Results The biomechanical strength, rigidity and stability in DP group and LCP group were better than those in GP group (P<0.05), while there was no statistical difference between LCP group and DP group (P>0.05). Conclusions Locking compression plate and modified dual plate are fairly ideal internal fixators for treatment of complex tibial plateau fractures. In the meantime, locking compression plate emphasizes conservation of soft tissues and blood supply, can better meet the requirement of the biological fixation of fracture and is the most ideal internal fixator at present.
7.Innova 3D-guided percutaneous vertebraplasty puncture reduces bone cement breakage
Songhua LI ; Kai YANG ; Yulan SHEN ; Qinghua TIAN ; Chungen WU
Chinese Journal of Tissue Engineering Research 2015;19(16):2473-2478
BACKGROUND:Percutaneous vertebraplasty is a new means for the treatment of vertebral compression fractures and bone metastases. The multiplaner reconstruction with digital substraction angiography (DSA)-Innova 3D plays an important role in guiding the percutaneous vertebraplasty puncture, which can effectively prevent the leakage of bone cement. OBJECTIVE:To explore the value of DSA-Innova 3D in the guidance of puncture of percutaneous vertebroplasty. METHODS:From March 2014 to September 2014, a total of 72 patients with 94 vertebras were selected for percutaneous vertebraplasty, of whom 36 (48 vertebras) with Innova 3D (experimentalgroup) and 36 (46 vertebras) without Innova 3D (control group). Spiral CT scan was performed in al patients to assess the distribution of bone cement. The imaging quality in the two groups was compared by Mann-Whitney U test. RESULTS AND CONCLUSION:CT showed bone cement leakage occurred in 7 of the 36 patients in the control group. Leakages were into the lumber disc in two cases, paravertebral space in two cases, vertebral pedicle in onecase or veins in two cases. However, only one case of bone cement leakage was observed in the experimental group. With the guidance of DSA-Innova 3D, the experimental group had signficantly lower leakage and fluoroscopic time than the control group (P < 0.05). The multiplaner reconstruction with DSA-Innova 3D plays an important role in guiding the percutaneous vertebraplasty puncture, thereby effectively reducing the leakage of bone cement.
8.Prokaryotic expression of Staphylococcus aureus Clumping factor B and evaluation of the antiserum-mediated opsonic activity.
Lili ZHANG ; Shanshan CAO ; Yifeng ZHANG ; Chunling XUAN ; Youqiang WU ; Songhua HU
Chinese Journal of Biotechnology 2015;31(11):1623-1631
Staphylococcus aureus is a major cause of hospital-acquired infection. Because the bacteria are very easy to become resistant to antibiotics, vaccination is a main method against S. aureus infection. Clumping factor B (ClfB) is an adhesion molecule essential for S. aureus to colonize in the host mucosa and is regarded as an important target antigen. In this study, we successfully used Escherichia coli to express a segment encoding the N1-N3 regions of ClfB protein (Truncated-ClfB) cloned from S. aureus. The protein was purified by affinity and ion exchange chromatographies and gel filtration. Rabbits were immunized three times with purified Truncated-ClfB. After that, blood was collected to prepare serum which were then used for measurement of antibody level. Phagocytosis of S. aureus opsonized by the serum was determined by a flow cytometry. Results show that the serum IgG titer reached 1:640 000. Phagocytosed S. aureus by polymorphonuclear leukocytes were significantly more when the bacteria were opsonized by the serum from Truncated-ClfB immunized rabbits than those from no immunized group (P < 0.01). Therefore, the results indicated that Truncated-ClfB could be a promising vaccine candidate against S. aureus infection.
Adhesins, Bacterial
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immunology
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Animals
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Antibodies, Bacterial
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blood
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Escherichia coli
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Flow Cytometry
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Immune Sera
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Immunoglobulin G
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blood
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Opsonin Proteins
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immunology
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Phagocytosis
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Rabbits
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Staphylococcal Infections
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immunology
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Staphylococcus aureus
9.CT imaging of bronchus related to solitary pulmonary lesion: comparison of minimum intensity projection and multi-planar reconstruction
Jun ZHOU ; Fei SHAN ; Zhiyong ZHANG ; Shan YANG ; Xingwei ZHANG ; Dong WU ; Songhua ZHAN
Chinese Journal of Radiology 2011;45(7):641-645
Objective To investigate the clinical value of 64-slice computed tomography with MinIP and MPR for imaging the bronchus related to a solitary pulmonary lesion (SPL). Methods Seventy-five subjects with solitary pulmonary lesions underwent chest 64-slice CT and their bronchi were analyzed retrospectively. All images of thin-section (0.625 mm) were reconstructed with MPR and MinIP into images of 1, 2, 3, and 5 mm thickness and 1 mm gap in two orthogonal planes along the long axis of bronchus related to the SPL. The image quality of four series of MinIP and MPR images was evaluated in the aspect of bronchus visibility and pulmonary vascular masking. One-way ANOVA with Bonferroni correction and interclass correlation coefficient were used in the statistical analysis. Results (1) The mean scores of display of the bronchi on MinIP images of four series (4.85, 4.77 and 4.84, 4.63 and 4.67, 4.25 and 4.28, in 1, 2, 3, and 5 mm thickness, respectively) and on MPR images of 1 or 2 mm thickness (4.77 and 4.76, 4.04 and 4.27, in 1 and 2 mm thickness, respectively) were good or excellent. MPR images of 1 mm thickness and MinIP images of 1-3 mm thickness showed no significant differences (t=0.318, P> 0.05 for all), but they were superior to MinIP images of 5 mm thickness (t=6.318 and 6.610, P<0.01). MPR images of 2 mm thickness were inferior to MinIP images of 1-3 mm thickness (t=5.003-8.958, P<0.01), but there was no significant difference between MPR images of 2 mm thickness and MinIP images of 5 mm thickness (t=1.794 and 0.3181, all P> 0.05). (2) The effect of suppression of pulmonary vascular markings on MinIP images was better with the increase of slice thickness (F=45.312 and 40.415, P<0.01). The mean scores of MinIP images of 3 mm and 5 mm thickness (4.67 and 4.64, 5.00 and 4.97, for 3 and 5 mm thickness, respectively) were good or excellent, but MinIP images of 2 mm thickness were just acceptable. Conclusion MinIP images of 3 mm thickness may display the bronchus related to SPL more clearly.
10.Approach to the patient with empty sella combined with adrenal adenoma
Junfeng HAN ; Mingxi XU ; Fang LIU ; Yuqian BAO ; Songhua WU ; Weiping JIA
Chinese Journal of Endocrinology and Metabolism 2013;29(8):711-713
Clinical characteristics were analyzed retrospectively in a patient with empty sella and adrenal adenoma with regard to the elaborated diagnosis and treatment.Although empty sella syndrome alone was common,clinical combination with adrenal adenoma was rarely reported.It was difficult to diagnose due to complex symptoms and hormone levels.This case could help increase awareness of the disease and accumulate the experience in diagnosis and treatment.