ObjeclJve To definine the corresponding value to glycated albumin(GA)for a specific target of HbAlc,and to elvaluate the relationship between GA and HbA1c.Methods From Oct.2006 TO Apr.2009, 2 532 subjects were enrolled who accepted oral glucose tolerance test(OGtt)in out-patient department,including 898 with normal glucose regulation,695 with impaired glucose regulation,and 939 with newly-diagnosed diabetes.GA was measured with liquid enzymatic method.HbA1c was measured with high performance liquid chromatography method.The plasma glucose was measured at fasting,0.5 h,1 h,2 h,and 3h after glucose load.The correlation among GA,HbA1c and the other parameters monitored was analyzed.Results (1)The levels of HbA1c and GA in 2 532 subjects were(6.3±1.1)% and(17.9±4.5)%.The ratio of GA/HbA1c was 2.85±0.51.(2)HbAlc and GA were positively correlated with fasting,0.5 h,1 h,2 h and 3 h plasma glucose(r was in 0.567-0.776,atl P<0.01).(3)GA was significantly correlated with HbA1c(r=0.701,P<0.01).Linear regression analysis,using GA and HbA1c summarized by patient(n=2 532),produced a relationship of GA=2.871×HbA1c-0.112.The change in GA per increase of 1% HbA1c was 2.87%.When HbA1c level was 6.5%,the expected value of GA was 18.5%.The sensitivity,specificity,positive predictive value,negative predictive value,and accuracy with GA≤18.5% to predict HbA1c≤6.5% were 82.32%,72.49%,86.48%.65.73%,and 79.19%,respectively.When HbA1c level was 7.0%,the expected value of GA was 20.O%.When HbA1c level was 7.5%.the expected value of GA was 21.4%.Conclusions We initially establish the corresponding value to GA for a specific target of HbA1c and provide the basis for clinical application.

Objective To establish a stable and feasible animal model of acute cerebral infarction,and to evaluate it with functional magnetic resonance imaging and pathology.Methods Twenty-five S-D rats were randomly divided into five groups,and there were 5 rats in each group.Rats in group A were sham-operated for control study.Unilateral middle cerebral artery occlusion(MCAO) was performed with improved thread in group B,C,D,E and were enrolled for MRI and MRS study at 3,6,12,24 hours after MCAO.All rats were examined by 1-hydrogen magnetic resonance spectroscopy(~1H MRS).Two or three rats in each group were sacrificed for 2,3,5-triphenyltetrazolium chloride(TTC) staining of the brain and two rats for pathological examination after MRS.Results Rats in group A showed no change in brain on ~1 H MRS or pathological study.~1H MRS of the rat brains after right MCAO showed a decrease of N-acetyl-aspartate(NAA),an increase of Lactate(Lac) in all groups.There was no significant change of Choline(Cho) and Creatine(Cr) peaks onrats in group B,C,D.The peaks of Cho and Cr were slightly dropped in group E.Conclusion The acute regional cerebral ischemic model in rats made by our approach is stable and reproducible,and it is suitable for evaluation and study with functional magnetic resonancespectroscopy accurately and sensitively.

Objective To explore the applications and effect of experience-based health education in maternal nursing in community.Methods According to the random number table,150 women pregnant for the first time were divided into control group and observation group with 75 in each group. In the control group,the traditional method for health education was used and in the observation group the experience-based teaching based on traditional health education.The two groups were compared in terms of the awareness rate of knowledge about maternal and infant health care and the incidence of postpartum depression.Result The awareness of knowledge about maternal and infant health care in the observation group was significantly higher than that of the control group and the incidence of postpartum depression in the observation group was significantly lower than that of the control group(P<0.01).Conclusion Experience-based health education can improve the level of knowledge about maternal and infant health care and reduce the incidence of maternal postpartum depression.

Objective To compare the differences of metabolic syndrome (MS) prevalence by using four working definitions and their relationship with obesity-related indicators in first-degree relatives of type 2 diabetes mellitus pedigrees. Methods Totally, 2 372 first-degree relatives from 715 type 2 diabetic pedigrees were selected in this study. Complete laboratory data, including blood pressure, lipid profile and plasma glucose, were collected. The prevalence rates of MS and obesity of four definitions, as defined by National Cholesterol Education Program Adult Treatment Panel Ⅲ (ATPⅢ) in 2005, International Diabetes Federation (IDF) in 2005,Chinese Diabetes Society (CDS) in 2004 aml Joint Committee for Developing Chinese Guidelines on Prevention and Treatment of Dyslipidemia in Adults (JCDCG) in 2007,were analyzed. Results (1)The prevalence rates of MS were 45.40% ,38.74% ,25.08% and 39.29% aecording to four definitions respectively. The prevalence rates of MS were higher in females than in males by using ATPⅢ and IDF definitions (both P<0. 01). (2)The common comhinations of metabolic abnormality was dyslipidemia, hypertension, obesity and hyperglycemia by using four definitions,except in females by using CDS definition. (3)The prevalence rates of obesity were 58.18% ,58.18% ,33.90% and 42.96% acconling to the four definitions respectively. The prevalence rates of MS in obese subjects were 66.59% ,66.59% ,54.85% and 68.99% according to four definitions respectively. (4) Applying the cutoff point for abdominal obesity according to ATPⅢ, IDF and JCDCG definitions, the prevalence rates of abdominal obesity in subjects with body mass index (BMI) <25 kg/m2 were respectively 28.58% and 16.78%, being higher in females than in males(38.90% vs 15.02% ,21.01% vs 11.22% ,both P<0. 01). Conclusion (1)There is significant familial aggregation of MS and obesity,and the first-degree relatives of type 2 diabetic patients are high risk populations. (2) Waist circumference rather than BMI taken as a discriminating component of obesity in MS seems to be clinically more helpful to the early identification and prevention of MS.

Bilateral middle pontine brachium and cerebral peduncula infarction is an extremely rare posterior circulation infarction.This article analyzed a patient with bilateral middle pontine brachium and cerebral peduncula infarction, reviewed the literature, and determined the cause through detailed medical history and imaging examinations.

Objective To explore the effect of drug-psychology combined with analgesia on preoperative pain and psychological state in patients with degenerative scoliosis.Methods Thirty-two patients with degenerative scoliosis from January 2010 to December 2012 received the drug-psychology combined with analgesia before the operation,and were surveyed by the Visual Analogue Scale (VAS),Self-Rating Anxiety Scale (SAS),Medical Coping Modes Questionnaire (MCMQ) in order to analyze the pain status and psychological state before and after the analgesia.Results The scores of VAS and SAS were respectively (5.23 ± 1.74),(32.60 ± 3.71) at admission,and were (2.34 ± 1.22),(25.50 ± 3.70) after the combined analgesics,and the differences were statistically significant (t =-5.23,-4.42,respectively;P ＜0.01).The further investigation on the anxiety showed that the top three factors were respectively the worry about operation,anesthesia and prognosis,the lack of the disease-related knowledge and the fear of intensive care unit after the operation.The scores of MCMQ including face,yield,avoidance were respectively (19.52 ± 3.04),(17.16 ± 2.17),(11.98 ± 4.75) at admission,and were (23.52 ± 3.46),(14.16 ± 2.14),(8.98 ± 1.75) after the analgesics,and the differences were statistically significant (t =5.09,-4.92,-4.16,respectively ; P ＜ 0.01).Most of patients could take active coping mode.Conclusions Drug-psychology combined with analgesia can effectively alleviate pain,anxiety and negative medical coping modes induced by preoperative pain,and make patients take active coping modes,and promote the physical and psychological rehabilitation of patients with degenerative scoliosis.

Objective To explore the correlations between serum soluble growth stimulation gene 2 protein(sST2),brain natriuretic peptide(BNP)and the degree of carotid atherosclerosis in patients with acute ischemic stroke.Methods A total of 205 patients with acute ischemic stroke were selected as study group,and were divided into mild group with 100 cases,moderate group with 70 cases,and severe group with 35 cases according to the severity of carotid atherosclerosis.Another 150 healthy people who received physical examination during the same period were selected as control group.Serum levels of sST2 and BNP were compared among all groups.The correlations of sST2 and BNP levels with the degree of carotid atherosclerosis were analyzed.The influencing factors of different degree of carotid atherosclerosis in patients with acute ischemic stroke were analyzed.The predictive value of sST2 combined with BNP on the degree of carotid atherosclerosis in patients with acute is-chemic stroke was analyzed.Results The serum sST2 level of the study group was significantly lower than that of the control group,and the serum BNP level was significantly higher than that of the control group(P＜0.05).The sST2 levels in moderate and severe groups were significantly low-er,BNP levels were significantly higher than that in the mild group(P＜0.05).BNP level in the severe group was significantly higher,sST2 level was significantly lower than that in the moderate group(P＜0.05).The degree of carotid atherosclerosis in patients with acute ischemic stroke was correlated with age,history of hypertension,history of smoking,history of diabetes,levels of total cholesterol(TC),C-reactive protein(CRP),homocysteine(Hcy)and low density lipoprotein(LDL)(P＜0.05).Age,hypertension history,smoking history,diabetes history,TC,CRP,Hcy,LDL,BNP and sST2 were the influencing factors for degree of carotid atherosclerosis(P＜0.05).Serum BNP level was positively correlated with the degree of carotid atherosclerosis,while sST2 level was negatively correlated with the degree of carotid atherosclerosis(P＜0.001).The ar-ea under the curve(AUC)for the combined prediction of sST2 and BNP was significantly greater than that for the individual prediction of sST2 and BNP(P＜0.001).Conclusion Clinical obser-vation of the changes in serum levels of sST2 and BNP in patients with acute ischemic stroke can ef-fectively assess the progression of the disease,which is beneficial for improving the severity of carot-id atherosclerosis in patients.The combined assessment of sST2 and BNP shows good diagnostic per-formance for evaluating the severity of carotid atherosclerosis in patients with acute ischemic stroke.

Objective To explore the correlations between serum soluble growth stimulation gene 2 protein(sST2),brain natriuretic peptide(BNP)and the degree of carotid atherosclerosis in patients with acute ischemic stroke.Methods A total of 205 patients with acute ischemic stroke were selected as study group,and were divided into mild group with 100 cases,moderate group with 70 cases,and severe group with 35 cases according to the severity of carotid atherosclerosis.Another 150 healthy people who received physical examination during the same period were selected as control group.Serum levels of sST2 and BNP were compared among all groups.The correlations of sST2 and BNP levels with the degree of carotid atherosclerosis were analyzed.The influencing factors of different degree of carotid atherosclerosis in patients with acute ischemic stroke were analyzed.The predictive value of sST2 combined with BNP on the degree of carotid atherosclerosis in patients with acute is-chemic stroke was analyzed.Results The serum sST2 level of the study group was significantly lower than that of the control group,and the serum BNP level was significantly higher than that of the control group(P＜0.05).The sST2 levels in moderate and severe groups were significantly low-er,BNP levels were significantly higher than that in the mild group(P＜0.05).BNP level in the severe group was significantly higher,sST2 level was significantly lower than that in the moderate group(P＜0.05).The degree of carotid atherosclerosis in patients with acute ischemic stroke was correlated with age,history of hypertension,history of smoking,history of diabetes,levels of total cholesterol(TC),C-reactive protein(CRP),homocysteine(Hcy)and low density lipoprotein(LDL)(P＜0.05).Age,hypertension history,smoking history,diabetes history,TC,CRP,Hcy,LDL,BNP and sST2 were the influencing factors for degree of carotid atherosclerosis(P＜0.05).Serum BNP level was positively correlated with the degree of carotid atherosclerosis,while sST2 level was negatively correlated with the degree of carotid atherosclerosis(P＜0.001).The ar-ea under the curve(AUC)for the combined prediction of sST2 and BNP was significantly greater than that for the individual prediction of sST2 and BNP(P＜0.001).Conclusion Clinical obser-vation of the changes in serum levels of sST2 and BNP in patients with acute ischemic stroke can ef-fectively assess the progression of the disease,which is beneficial for improving the severity of carot-id atherosclerosis in patients.The combined assessment of sST2 and BNP shows good diagnostic per-formance for evaluating the severity of carotid atherosclerosis in patients with acute ischemic stroke.

The setting and adjustment of ventilator parameters need to rely on a large amount of clinical data and rich experience. This paper explored the problem of difficult decision-making of ventilator parameters due to the time-varying and sudden changes of clinical patient's state, and proposed an expert knowledge-based strategies for ventilator parameter setting and stepless adaptive adjustment based on fuzzy control rule and neural network. Based on the method and the real-time physiological state of clinical patients, we generated a mechanical ventilation decision-making solution set with continuity and smoothness, and automatically provided explicit parameter adjustment suggestions to medical personnel. This method can solve the problems of low control precision and poor dynamic quality of the ventilator's stepwise adjustment, handle multi-input control decision problems more rationally, and improve ventilation comfort for patients.
Humans ; Ventilators, Mechanical ; Respiration, Artificial ; Neural Networks, Computer

Objective To screen long non-coding RNA(lncRNA)associated with disulfidptosis and investigate the immune landscape between lncRNA and pancreatic cancer,for effective guidance in clinical practice.Methods The normal and pancreatic cancer tissue samples were obtained from The Cancer Genome Atlas database,and the lncRNA associated with disulfidptosis was identified based on the Cox and LASSO regression analyses.A risk prognosis model was constructed,and its predictive performance was verified using comprehensive methods.An accurate nomogram was construted to predict the prognosis of patients with pancreatic cancer.The biological differences were analyzed via Gene Ontology,Gene Set Enrichment Analysis,and an immunoassay.The immunotherapy response was estimated using the tumor mutational burden(TMB)score.Results A total of 251 disulfidptosis-related lncRNAs were successfully identified,and three groups of lncRNAs were selected as the reference for the risk model.Pathway analysis showed that immune-related pathways were associated with disulfidptosis-related lncRNA risk models.The risk score was significantly correlated with immune cell infiltration and the ESTIMATE score.Patients with higher risk scores had elevated TMB,indicating that high-risk patients exhibited a better immune checkpoint blockade response.Conclusion The findings of this study contribute to a deeper understanding of disulfidpto-sis-related lncRNA and provide a potential therapeutic strategy for pancreatic cancer.