To retrospectively analyze the safety and efficacy of low dose subcutaneous decitabine regimen in patients with acute myeloid leukemia (AML) and intermediate-or higer-risk myelodysplastic syndrome (MDS).Of 6 AML cases,2 achieved complete remission (CR),2 with partial remission(PR),1 with stable disease(SD),1 with progressive disease(PD).As to the 8 MDS patients,one achieved CR and 6 with hematologic improvement (HI),1 case SD.Low dose subcutaneous decitabine regimen could be an alternative choice of older AML or MDS patients.

Objective:To prepare and identify the mouse anti-human monoclonal antibodies ( mAbs) using leukocytes as im-munogens. Methods: The mice were immunized using human peripheral blood leukocytes. Then, use of B lymphocyte hybridoma technology preparation of mAbs,followed screening by immunocytochemistry and limited dilution. The secreted mAbs were identified by immunoprecipitation,mass spectrometry,Western blot,ELISA and immunohistochemistry. Results:The 35 positive polyclonal cells were obtained,of which 11 strains secreted mAbs against S100A9. And one strain was used to prepare monoclonal antibody. The purified mAb against S100A9 were purified and identified as IgG1 subtype,with the titer,purity and affinity constant was 1∶3. 18×105,95% and 3. 54×108 L/mol,respectively. This mAb generally had 0. 12% crossed reactivity to S100A8 ,and showed little or no cross reactivity to S100A12 and S100A13. The prepared monoclonal antibodies can specifically recognizes the S100A9 antigen in human breast cancer tissues. Conclusion:Successful preparation of mAb against S100A9,which can secrete specific mAb against S100A9 protein with high titers and specificity have been established successfully,which laid the foundation for the immunology application.

Adult ; Child ; Eosinophilia ; complications ; Female ; Hematologic Neoplasms ; complications ; Humans ; Male ; Middle Aged

Objective To retrieve relevant evidence for perioperative nutritional management of elderly patients with hip fractures and summarize the best evidence.Methods Clinical decision-making,guidelines,evidence summaries,systematic reviews,expert consensus,and other relevant literature on perioperative nutritional management of elderly patients with hip fractures were retrieved from Chinese National Knowledge Infrastructure(CNKI),UpToDate,National Institute for Health and Care Excellence(NICE),Cochrane Library,Scottish Intercollegiate Guidelines Network(SIGN),National Guideline Clearinghouse,Chinese Medical Pulse Guideline Network,PubMed,VIP Biomedical Journal Database,ovid(Embase),Chinese Medical Journal Full-text Database,Chi-nese Biomedical Literature Database,and Wanfang Medical Network.The search period was from January 2015 to September 2023.Results A total of 13 articles were included in this study,inclu-ding 5 guidelines,4 expert consensus,and 4 systematic reviews.A total of 32 best evidences were summarized from six aspects:nutritional support goals,nutritional assessment and screening,preop-erative nutritional support,postoperative nutritional support,nutritional intervention principles,and diet management.Conclusion This study summarizes the best evidence for perioperative nutritional management of elderly patients with hip fractures,which can help healthcare professionals better im-plement effective perioperative nutritional management for elderly patients with hip fractures.

Objective To retrieve relevant evidence for perioperative nutritional management of elderly patients with hip fractures and summarize the best evidence.Methods Clinical decision-making,guidelines,evidence summaries,systematic reviews,expert consensus,and other relevant literature on perioperative nutritional management of elderly patients with hip fractures were retrieved from Chinese National Knowledge Infrastructure(CNKI),UpToDate,National Institute for Health and Care Excellence(NICE),Cochrane Library,Scottish Intercollegiate Guidelines Network(SIGN),National Guideline Clearinghouse,Chinese Medical Pulse Guideline Network,PubMed,VIP Biomedical Journal Database,ovid(Embase),Chinese Medical Journal Full-text Database,Chi-nese Biomedical Literature Database,and Wanfang Medical Network.The search period was from January 2015 to September 2023.Results A total of 13 articles were included in this study,inclu-ding 5 guidelines,4 expert consensus,and 4 systematic reviews.A total of 32 best evidences were summarized from six aspects:nutritional support goals,nutritional assessment and screening,preop-erative nutritional support,postoperative nutritional support,nutritional intervention principles,and diet management.Conclusion This study summarizes the best evidence for perioperative nutritional management of elderly patients with hip fractures,which can help healthcare professionals better im-plement effective perioperative nutritional management for elderly patients with hip fractures.

Objective:To construct an evaluation index of discharge readiness suitable for elderly patients after hip fracture surgery in China, so as to provide a basis for the implementation of discharge readiness services for elderly patients after hip fracture surgery.Methods:From January 2018 to March 2023, a preliminary version of the discharge readiness evaluation indicators for elderly patients with hip fracture was constructed through literature search and semi-structured interviews. From April to May 2023, the Delphi method was used to select 19 experts from the medical and nursing fields to conduct two rounds of expert inquiries on various indicators. The final version of the discharge preparation evaluation indicators for elderly hip fracture patients was constructed through indicator screening and modification.Results:Through literature search and reading, 13 literatures were obtained. A total of 101 expert opinions were collected in two rounds of expert consultation, and the participation rate and questionnaire recovery rate of the two rounds of expert consultation were 100.00% (19/19). The judgment coefficient and familiarity coefficient of the two rounds of expert consultation were 0.979 and 0.832, respectively, and the expert authority coefficient were both 0.905.The Kendall's harmony coefficients of the two rounds of expert correspondence were 0.154 and 0.157, respectively ( P<0.01). The final evaluation index of discharge readiness of elderly patients with hip fracture included three primary indexes and 12 secondary indexes. Conclusions:The evaluation index of discharge readiness of elderly patients after hip fracture surgery established in this study is scientific and reliable, providing a basis for accurately assessing discharge readiness of elderly patients after hip fracture surgery.

Objective:To construct an ultra-early limb movement program for elderly hip fracture surgery patients based on evidence-based practice and the Delphi method.Methods:From May to December 2023, the PIPOST Model (population, intervention, professional, outcome, setting, type of evidence) was used to develop evidence-based questions. Relevant databases were searched to obtain, summarize, and evaluate evidence related to ultra-early limb movement after hip fracture surgery in elderly patients. A draft of the movement program was created with the search timeline set until June 1, 2023. A total of 29 experts were invited to participate in two rounds of Delphi expert consultation to finalize the ultra-early limb movement program for elderly hip fracture surgery patients.Results:A total of 20 studies were included, consisting of six guidelines, four expert consensuses, three systematic reviews, and seven original research articles. The response rates for the two rounds of Delphi expert consultation were 100.00% (29/29) and 86.21% (25/29). The expert authority coefficients were 0.931 and 0.920, and the Kendall coordination coefficients were 0.205 and 0.112 (all P<0.01). The final ultra-early limb movement program for elderly hip fracture surgery patients included four modules and 39 operational items. Conclusions:This study develops an evidence-based, expert-validated ultra-early limb movement program for elderly hip fracture surgery patients, demonstrating its necessity, scientific basis, and practicality. The program provides a reference and guidance for clinical staff in facilitating ultra-early movement for hip fracture patients post-surgery.

OBJECTIVETo establish a comprehensive quality control method for total flavonoid of Fructus Aurantii.

METHODSRP-HPLC and spectrophotometry were applied for the quantitative and fingerprint analysis of total flavonoid of Fructus Aurantii. The contents of naringin and neohesperidin were determined on an Agilent SB-C₁₈column (4.6 mm × 250 mm, 5 μm). The mobile phase was composed of 0.02 % H₃PO₄ and CH₃CN (80:20). The flow rate was 1 ml/min with DAD detected at 280 nm. The column temperature was maintained at 35°C. The fingerprints were developed on an Agilent SB-C₁₈ column (4.6 mm × 250 mm, 5 μm). The mobile phase was composed of 0.5 % HAc and CH₃OH with a linear gradient elution. The ratio of 0.5 % HAc and CH₃OH was: 0 min, 80:20; 10 min, 60:40; 35 min, 30:70; 50 min, 0:100. The flow rate was 1 ml/min with DAD detected at 320 nm. The column temperature was maintained at 30 degree. Meanwhile, the contents of total flavonoid were determined at 283 nm.

RESULTThe contents range of naringin, neohesperidin and total flavonoid were 38.3 %- 47.2%, 21.0 %- 28.5% and 79.9%-88.6 %, respectively. The fingerprints of the effective fractions showed 12 common peaks and the fingerprint similarity was all above 98.0 % compared with the standard chromatogram.

CONCLUSIONThe method reported in this paper can be used effectively for the quality control of total flavonoid of Fructus Aurantii.

Objective: To analyze the efficacy and safety of asparaginase based chemotherapy bridging autologous hematopoietic stem cell transplantation (auto-HSCT) in the treatment of 16 patients with nasal type extranodal NK/T-cell lymphoma (ENKTL). Methods: From January 2012 to June 2017, 16 patients with nasal type extranodal NK/T-cell lymphoma reached complete remission by L-asparaginase based regimens, and then received auto-HSCT. Results: ①Of the 16 patients, 12 were males and 4 females, with a median age of 35.5 (14-61) years. There were 11 patients in the first complete remission (CR1) and 5 in the second CR (CR2) before transplantation, respectively. EB virus (EBV) DNA (EBV-DNA) was negative and positive in 13 and 3 cases respectively before transplantation. ②Hematopoietic reconstitution was achieved in all 16 cases. The median time for neutrophils implantation was 12 (8-17) days, and that of platelet implantation was 15.5 (12-24) days. ③To the last follow-up, there were no transplant related deaths, 3 patients died of disease progression. The median overall survival (OS) time and progression-free survival time (PFS) were not reached. Seven patients lived with no disease progression more than 2 years. ④The OS and PFS of patients at CR₁ before auto-HSCT are better than that of patients at CR₂, but there was no statistically significant difference (P=0.162, P=0.123). There was no significant difference in OS and PFS between EBV-DNA negative and positive patients before transplantation (P=0.280, P=0.244). Conclusions L-asparaginase based regimens bridging auto-HSCT is a safe and highly effective for advanced-stage and relapsed ENKTL treatment.

Falls among the elderly are a serious public health problem. This paper reviews the application status of machine learning in fall risk identification, fall prevention training intervention, fall behavior detection and warning, analyzes the advantages and disadvantages of machine learning in the field of fall risk management for the elderly, and aims to provide ideas for personalized and accurate fall prevention practice for the elderly in the future.