Objective To study the technique condition for separating and purifying total flavones from Striga asiatica (L.) O. Ktze. by Polyamide. Methods Total flavones content, in the sample solution of Striga asiatica (L.)O. Ktze. , detected by UV spectrophotometry, is used as the index. Some technological parameters are observed by single factor observation. Results Polyamide has good absorbing effect on total flavones of Striga asiatica (L.) O. Ktze. The liquid concentration of its absorbing and separating technological condition is 1.12～2. 24 mg/mL and at the absorbing speed of 2BV/h. The elution effect of 95 % alcohol of 250 mL is the best. Conclusion This method is simple and feasi-ble, fit for separating and purifying total falvones from Striga asiatica (L.) O. Ktze.

Objective To explore the essence of polycystic ovary syndrome of spleen-kidney yang deficiency,and to investigate the objective standardization of TCM syndrome diagnosis of the disease.Methods Among the 200 PCOS patients,the Chinese medicine syndrome pattern was differentiated as spleen-kidney yangdeficiency type (n=105 )and Non-spleen-kidney yang deficiency type (n=95).To measure and compare their levels ofBMI、WHR、sexual hormones、FINS、2 hINS,and HOMR-IR.Results The levels of BMI (24.4±2.8)、WHR (0.88±0.07)、LH/FSH (2.02±1.06)、E2 (55.84±23.87) pg/ml、HOMR-IR (2.95±1.52)、FINS (15.18±7.64) IU/ml、2hINS ( 59.53 ±40.61 ) IU/ml in the group of spleen-kidney yang deficiency type were obviously higher than those[ (22.2±2.2)、(0.80±0.06)、(3.50± 1.80)、(45.82±21.31) pg/ml、( 1.90± 1.50)、(9.93±6.59) IU/ml、(50.56±41.34) IU/ml respectively] in the group of Non-spleen-kidney yang deficiency type (P＜0.05＝,but T and FSH level showed no significant difference between two groups [FSH the group of spleen-kidney yang deficiency type=(6.46±0.95) IU/L、FSH the group of No-spleen-kidney yang deficiency type=(6.40±1.16) IU/L; T the group of spleen-kidney yang deficiency type=(0.81±0.15) μg/L、T the group of No-spleen-kidney yang deficiency type=(0.72±0.13) μg/L,P＞0.05].Conclusion BMI、WHR、LH/FSH、E2、FINS、2HINS,and HOMR-IR could be used as objective index in identifying the difference in between spleen-kidney yang deficiency type and Non-spleen-kidney yang deficiency type.

OBJECTIVE: To establish the quality standard of Lithocarpus polystachys.METHODS: TLC was used for the qualitative identification of L.polystachys.The content of total flavonoids of L.polystachys was determined by UV spectrophotometry and the content of phloridzin was determined by HPLC.RESULTS: The TLC spots of polyamide were clear and well separated.The maximum absorption wavelength of phlorizin was 284 nm.The content of total flavonoids of 6 batches of L.polystachys ranged from 103.12 mg?g-1 to 183.54 mg?g-1.The linear ranges of phlorizin were 0.099 8～1.197 6 ?g (r=0.999 7) with an average recovery of 97.23%(RSD=1.57%,n=6).CONCLUSION: Established quality standard is applicable for the quality control of L.polystachys.

Objective To determine the malignant grade related proteins implicated in human astrocytoma with proteomic techniques. Methods Two-dimensional electrophoresis (2-DE) was used to compare the protein expression profiles of normal human brain tissues (n=12) and astrocytoma tissue samples of various malignant grades (23 with grade Ⅱ, 15 with grade Ⅲ and 14 with grade Ⅳ ).After identifying the differential protein spots, electrospray ionization (ESI) tandem mass spectrum and bioinformatics were employed to determine the protein ID. Results A total of 15 proteins with differential expressions were identified,which included 10 up-regulated ones (heat shock protein 27,prohibitin,78-kDa glucose-regulated protein,peroxiredoxin 1,peroxiredoxin 6,annexin Ⅱ,cathepsin D,plasminogen activator inhibitor-1, actin [cytoplasmic 1], Glutathione S-transferase M) and 5 down-regulated proteins (disulfide isomerase A3 protein,alpha-crystallin B chain protein,T-complex protein 1 ε subunit,ubiquitin carboxyl-terminal hydrolase isozyme L1 and glial fibrillary acidic protein) in astrocytoma tissues of grade Ⅲ and Ⅳ. Conclusion Proteomic techniques can screen the malignant-grade associated proteins effectively,which provides rationale for individualized diagnosis and therapeutic targets of astrocytoma.

Objective To explore the effect of small interfering RNA (siRNA)-mediated cathepsin D (CD) knock-down on biological behavior of U87 cells. Methods CD-siRNA,scramble siRNA and control siRNA mediated by lipofectamin were introduced into the U87 cells,respectively.The silencing effect of CD was verified by RT-PCR and Western blotting in terms of CD mRNA and protein expressions. The cell viability of scramble and CD siRNA treated U87 cells was measured by MTT on 1-5 d after transfection. The invasive potential of scramble and CD siRNA treated U87 cells was measured by Transwell coated with Matrigel 48 h after transfection. Results Forty-eight h after transfection, mRNA and protein expressions of CD in CD siRNA treated U87 cells were remarkably reduced as compared with those in the scramble siRNA and control siRNA treated U87 cells (P＜0.05).The absorbance (A value) of CD siRNA treated U87 cells was significantly lower than that in the scramble siRNA treated U87 cells 2,3,4 and 5 d after transfection (P＜0.05); and the invasive potential of CD siRNA treated U87 cells was suppressed significantly as compared with that in the scramble siRNA treated U87 cells (P＜0.05). Conclusion CD has the potential to be a promising therapeutic target.

OBJECTIVETo observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.

METHODSRat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.

RESULTSThe PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.

CONCLUSIONBone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts

Animals ; Bone Marrow Cells ; pathology ; Cell Differentiation ; physiology ; Environment ; Hematopoietic Stem Cells ; pathology ; Hepatocytes ; pathology ; Liver Cirrhosis ; pathology ; Male ; Rats ; Rats, Sprague-Dawley

BACKGROUNDIt has been widely observed that infants and young children can reossify large calvarial defects when they are younger than 2 years of age; afterwards, they lose this regenerative potential. Previous studies have implicated that the dura mater serves as a key regulator of calvarial regeneration. However, the molecular mechanism of calvarial reossification remains elusive.

METHODSIn order to identify the proteins that may participate in this process, we performed a proteome-wide comparison of the protein expression levels of immature and mature dura using 2D electrophoresis and MALDI-TOF mass spectrometry. The Western blotting was used to verify the results of the 2D electrophoresis/MALDI-TOF mass spectrometry.

RESULTSEleven proteins were found to express with significant differences in the immature and the mature dura. Among them, the emergence of vimentin, tropomyosin, beta-actin and gamma-actin were further confirmed by the Western blotting analysis.

CONCLUSIONThe proteins and proteomic profiles provide a better understanding of the molecular mechanism of calvarial regeneration.

Aging ; physiology ; Animals ; Blotting, Western ; Dura Mater ; chemistry ; Electrophoresis ; Mass Spectrometry ; Proteins ; analysis ; Proteomics ; Rats ; Rats, Sprague-Dawley

Adult ; Aged ; Budd-Chiari Syndrome ; diagnostic imaging ; Female ; Humans ; Liver ; diagnostic imaging ; Liver Circulation ; Male ; Middle Aged ; Ultrasonography, Doppler, Color ; Young Adult

OBJECTIVETo investigate the effects of Carthamus tinctorius on bcl-2, caspase-3 expression of apoptosis of neurons.

METHODSD rats were randomly divided into ischemia control group, large-dose group, middle-dose group and low-dose group. The middle cerebral artery of rats was occluded for 2h by inserting an introluminal molofilament, and reperfusion was then instituted for 4h or 22h. The brains were stained with 2, 3, 5-triphenylte trazolinm chloride for assessment of volume of infarction, and then embedded onto slides with paraffin for morphological assessment and immunohistochemistry was carried out to investigate the changes in bcl-2 and caspase-3.

RESULTAll treated groups at different times decreased the volume of infarction (P < 0.05), while large-dose group showed more distinct decrease than other groups (P < 0.05). All treated groups at different times increased bcl-2 and decreased caspase-3 expression as well, while large-dose group showed more distinct effect (P < 0.05).

CONCLUSIONC. tinctorius injection can reduce the volume of cerebral infarction, and increased bcl-2 and decreased caspase-3 expression.

Animals ; Apoptosis ; drug effects ; Brain ; metabolism ; pathology ; Carthamus tinctorius ; chemistry ; Caspase 3 ; Caspases ; metabolism ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; Female ; Infarction, Middle Cerebral Artery ; metabolism ; pathology ; Male ; Neurons ; cytology ; metabolism ; Plants, Medicinal ; chemistry ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; metabolism ; pathology

BACKGROUNDAlthough tongue cancer is a common disease of the head and neck, the choice of neck treatment between elective neck dissection and "watchful waiting" remains controversial for patients with early stage N0 oral tongue carcinoma.

METHODSOn the basis of the current state of head and neck cancers a decision analysis model was created to compare two treatment strategies for early tongue cancer. Expected value (EV) was calculated according to the literature which met the defined criteria. Sensitivity analyses were performed.

RESULTSThe results showed that the decision model favored elective neck dissection (EV = 0.87), over "watchful waiting" (EV = 0.77). One-way sensitivity analyses demonstrated that the outcome was influenced by regional recurrence, threshold value of 0.28 for the elective neck dissection group and 0.17 for the "watchful waiting" group, and a salvage rate threshold value 0.73 for the "watchful waiting" group.

CONCLUSIONSThese results suggested that elective neck dissection strategy of the neck should be applied for early stage N0 oral tongue carcinoma patients with no clinical nodal metastases. When the occult lymph node metastases rate was less than 0.17 and the salvage rate was more than 0.73, "watchful waiting" strategy would be preferable.

Carcinoma, Squamous Cell ; pathology ; surgery ; Decision Support Techniques ; Elective Surgical Procedures ; Humans ; Neck Dissection ; methods ; Neoplasm Recurrence, Local ; Neoplasm Staging ; Tongue Neoplasms ; pathology ; surgery