The optimal distance from tumor to incision varied according to the cellular type of the tumor. In each case the cutting edge of the excised tissue was checked with frozen sections for cancer cells. Multiple neighboring skin flaps in full thickness raised from the periosteum were used to cover the large defects (the smallest defect is 25 20mm, the largest one is 75 65mm). The results showed that good cosmetic results were obtained in all cases, visual function was preserved in 35 of the 36 patients, and no recurrence of the tumor was found in 5 years' follow-up. One patient with squamous cell carcinoma that disseminated to nasal cavity and sinuses had a complication of meningitis after the operation,and it was cured with proper treatment.

OBJECTIVE:To prepare domiphen bromide osmotic tablets,and to observe,analyze the mechanism of drug release.METHODS:Coating prescription was optimized by uniform design and the cumulative rates of drug release of different formulated preparations were measured,pharmaceutical features of domiphen bromide osmotic tablet and mechanism of drug release were studied.RESULTS:The dosage of PEG-400 and the coating membrane thickness all had an effect on drug release.The optimal coating technique was analyzed as 12%of PEG-400 and 10mg of coating.Spray speed,temperature and rotation speed did not affect releasing profiles.CONCLUSION:Improvement in formulation of coat of domiphen bromide osmotic tablet can result in drug release for 12h,whose mechanism includes diffusion and osmotic pump principle.

OBJECTIVE: To investigate the effect of enzymatically modified low density lipoprotein(E-LDL) on differation and maturation of human dendritic cells(DC) in peripheral blood and the effect of diltiazem on it.METHODS: Human monocytes were isolated by using gradient centrifugation method and cultured in DC Cellgro medium containing rhGM-CSF and rhIL-4 for 5 days,then DC was then obtained.DC were treated with E-LDL or E-LDL plus diltiazem for 48 hours,the morphological changes were observed under the microscope,and the FACS was used to investigate the expression of DC(CD1a,CD80,CD86 and HLA-DR).PBS group was set up for comparison.RESULTS: The morphological changes were nor observed.The expressions of CD1a,CD80,CD86 and HLA-DR in each groups were significant different(P

Objective To optimize the method of isolating ,culturing and screening fetal mouse liver stem cells in vitro ,and to identify the potential of bi‐directional differentiation .Methods The fetal liver stem cells of mouse were isolated by the density gra‐dient centrifugation and cell difference adherence method ,the proliferation of stem cells was determined by cell plate cloning tech‐nique and MTT method;stem cells were induced for differentiation by adding DMSO and HGF .Results The isolated stem cells showed adherence within 24 h ,which were orbicular‐ovate ,closely packed ,activated within 1~2 weeks ;the positive rates of CD133 , CD49f and EPCAM were (97 .95 ± 1 .21)% ,(92 .71 ± 3 .49)% and (50 .73 ± 3 .45)% respectively ;AFP and CK19 proteins were expressed;red glycogen granules were seen by PAS after induced differentiation;ALB and HNF‐4αwere expressed .Conclusion Fe‐tal hepatic stem cells are successfully isolated by the density gradient centrifugation combined with difference adherence method ,and the isolated cells have strong stemness and proliferation ability ,as well as the ability of bi‐directional differentiation towards hepato‐cytes and bile duct epithelial cells .

Objective To prepare Domiphen osmotic tablets of zero release above 80% in vitro. Methods Coating prescription was optimized by uniform design and the drug release characteristic was tested in vitro. Results The lemma thickness was (shown as lemma weight) 10.75 mg and the content of the porosity-making agent was 12%. The three batchs of drug tablets release pattern were in accord with zero order kinetics during 12 h. Conclusion This method is easy to produce and reaches initial devise request.

OBJECTIVEThis study aims to test the accuracy and precision of iWitness photogrammetry for measuring the facial tissues of mannequin head.

METHODSUnder ideal circumstances, the 3D landmark coordinates were repeatedly obtained from a mannequin head using iWitness photogrammetric system with different parameters, to examine the precision of this system. The differences between the 3D data and their true distance values of mannequin head were computed.

RESULTSOperator error of 3D system in non-zoom and zoom status were 0.20 mm and 0.09 mm, and the difference was significant (P 0.05). Image captured error of 3D system was 0.283 mm, and there was no significant difference compared with the same group of images (P>0.05). Error of 3D systen with recalibration was 0.251 mm, and the difference was not statistically significant compared with image captured error (P>0.05). Good congruence was observed between means derived from the 3D photos and direct anthropometry, with difference ranging from -0.4 mm to +0.4 mm.

CONCLUSIONThis study provides further evidence of the high reliability of iWitness photogrammetry for several craniofacial measurements, including landmarks and inter-landmark distances. The evaluated system can be recommended for the evaluation and documentation of the facial surface.

Objective To investigate the effect of partial sleep deprivation (PSD) on cardiac electric activity and function of vascular endothelium. Methods Eighteen Sprague-Dawley rats were randomly divided into two groups (9 each):PSD group and control group. PSD lasted 10 days,20 hours each day. Body weight and surface electrocardiogram (S-ECG) were examined and recorded 1d before PSD,and 1d,4d,7d and 10d after PSD. Serum concentrations of glucose,total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C),creatin kinase (CK),creatin kinase-MB (CK-MB),high-sensitivity C-reactive protein (hs-CRP),nitric oxide (NO),von Willebrand factor (vWf),endothelin-1 (ET-1) and E-selectin were determined the next day after 10d of PSD. Results Compared to that 1d before PSD,body weight in PSD groups decreased significantly,while increased obviously in control group. Compared to that in control group,the body weight of rats decreased significantly (P

OBJECTIVE: To study the relationship among the dosage, serum concentration and clinical effect of phenytoin(PHT) in treating epileptic patients .METHODS: 130 steady -- state serum concentrations of PHT were determined by fluores-cence polarization immunoassay(FPIA) in 70 patients with or without other drugs used in combination .RESULTS: The indi-vidual difference among the patients who received routine dosage of PHT was very significant. Intoxication occurred ex-tremely often in patients whose PHT serum concentrations were over 30?g/ml .CONCLUSION: It is necessary to perform TDM(Therapeutic Drug Monitoring) and individualize the use of PHT,so that the safe and effective use of drug can be ensured.

OBJECTIVE To understand the epidemiological and clinical features of enteritis caused by Norwalk virus in the hospital and observe the effectiveness of prevention and control system in order to provide the basis for the control of outbreaks.METHODS By adopting field epidemiological and clinical data study,investigation and analysis were conducted on eight cases of suspected patients with enteritis caused by Norwalk virus in the hospital.Control on gastrointestinal infections and droplet infection was implemented.RESULTS Bacterial test on feces was conducted on eight cases of suspected gastroenteritis caused by Norwalk virus.Results were negative,thus diagnosed as viral infection;among them,virology test on feces was conducted on two cases which were diagnosed as enteritis caused by Norwalk virus.All patients were recovered after proper treatment and no new cases were found after timely disinfection.CONCLUSIONS The epidemic outbreak of enteritis caused by Norwalk virus has been timely and effectively controlled in the hospital;because communication channel on infection control management is open,contingency plans are effective and the prevention and control taskforce functions well.

Purpose To discuss the value of MRI in the diagnosis of placenta implantation abnormality, and to explore preliminarily the relationship between MRI signs and types of placenta implantation abnormality. Materials and Methods The clinical preoperative data and postnatal pathological findings of 54 women at high risk of placenta accreta were collected. All the patients undertook the conventional pelvic MRI examination. The scanning sequences mainly included: sagittal, coronal and axial T2-weighted imaging-turbo spin echo, balance fast field echo. The MRI images were observed and the areas which showed low signal in all the three directions on T2WI were measured. Then the correlation between the areas of low signal on T2WI in placental and the types of placenta implantation abnormality was analyzed. Results The incidence of placenta implantation abnormality was 64.8% in our research (35/54). The main MRI signs were low signal on T2WI (68.5%, 37/54) and heterogeneous signal in placenta (57.4%, 31/54); the main sign of placenta percreta was tenting bladder (75.0%, 6/8). The types of placenta implantation abnormality were positively correlated with the areas of low signal on T2WI (r=0.454, P<0.05). Conclusion Pregnant women at risk of placenta accreta should be evaluated with imaging examinations, particularly with MRI scanning, to improve disease detection rate. The typical indirect signs of placenta implantation abnormality are low signal on T2WI and heterogeneous signal in placenta. The larger size of low signal area on T2WI in placenta, the deeper implantation of placenta.