Objective To investigate the effects of traditional transmyocardial laser revascularization (T-TMLR) and non-transmural myocardial laser revascularization (N-TMLR) on myocardial ischemic and necrotic areas and angiogenesis. Methods In rabbit models, using Evans′ blue -TTC stain and HE stain technique for measuring ischemic and necrotic areas and observing angiogenesis. Results After T-TMLR and N-TMLR,compared with the control group, the ischemic areas significantly decreased and significant elevation of angiogenesis was observed. But no difference in necrotic areas was observed among the three groups. Conclusion N-TMLR can achieve the same effects with T-TMLR. Inducing angiogenesis via laser channels and decreasing ischemic area may be one of the predominant mechanisms of TMLR whereas laser channels themselves open or not has no relation to the effects of TMLR.

Objective To observe the effect of lattice silver wound repair dressings on large-area abrased wounds . Methods A total of 60 patients with large-area skin abrasions were randomly divided into the observation group and the control group with 30 cases in each group. Both groups were treated with the same antibacterial treatment and nutritional support treatment. The control group received conventional general routine dressings and the observation group had the silver wound repair dressings every day. The two groups were compared in terms of the wound pain, wound infection rate, time for wound healing and scars of patients. Results The wound healing time in the observation group was significantly shortened (P<0.01). The scaring of the observation group was better than that in the control group (P<0.01). The pain degree and the exudation rate were both significantly lower in the observation group than those of the control group (P<0.01), and wound infection rate was lower than that of control group. Conclusions The lattice silver wound repair dressing can effectively promote the wound healing, shorten hospitalization duration, promote the patient recovery soon. It is simple and worthy of clinical popularization.

Objective To study the biological effect of Icariine on human umbilical cord derived mesenchymal stem cells(huCMSCs) and inducing them to differentiate into osteoblasts. Methods MSCs were isolated and expanded in vitro and their surface antigens of huCMSCs were detected by immunohistochemistry. HuCMSCs were assigned into two groups. In the blank control group, huMSCs were incubated in basic medium. In the experimental group huMSCs were incubated in Icariine medium, The proliferation and differentiation of huMSCs were examined by inverted microscope, Alkaline phosphatase (ALP) staining and the determination of ALP activities. Results HuCMSCs were strongly positive for CD44, and negative for CD34; strongly positive expression for ALP and calcium deposition was detected from the second day to the fourth day in Icariine group; blank control group and Icariine group had significant differences (P ＜ 0.05).Conclusion HuCMSCs can be successfully cultured from the adherent tissue pieces, Icariine enhances proliferation and osteogenic differentiation of huCMSCs and be used as an osteoinductive factor.

Objective To evaluate the effect of different grades of pelvic inflammatory disease (PID)and of salpingectomy on outcomes of in vitro fertilization-embryo transfer(IVF-ET).Methods Two hundred and twenty three cycles of IVF-ET were divided into three groups,including mild group,moderate group and severe group,according to different grades of sequelae of PID finding in exploratory operations before IVF.Patients in each group were divided into two subgroups according to receiving salpingectomy or not.The data of total dose of gonadotrophin(Gn),oocyte number,low response rate,fertilization rate,good embryo number and pregnancy rate were analysed between three groups and the two subgroups of each group, respectively.Results All parameters were related to PID grades except fertilization rate.The total dose of Gn,oocyte number,good embryo number,low response rate and pregnancy rate were(2057?503)IU/L, (16?6),(6.0?4.3),4.2%,63.9% in mild group;(2204?603)IU/L,(12?6),(4.5?3.5), 13.9%,46.8% in moderate group;and(2372?1018)IU/L,(9?6),(3.1?2.9),33.8%,41.2% in severe group.The total dose of Gn and low response rate increased with the aggravation of the grades of PID (P

Objective To investigate arterial blood gas(ABG)changes induced by different intra- abdominal pressures during CO_2 pneumoperitoneum in rabbits with lung and liver impact injury under controlled hemorrhage.Methods In this study 75 rabbits were randomized into nine groups(6 rabbits in each group)according to the amount of blood loss(6 ml/kg、12 ml/kg、22 ml/kg)and intra-abdominal pressures(5 mm Hg,10 mm Hg,15 mm Hg).After model was established successsfully,respiratory rates (RR),ABG and death rates were observed at pre-pneumoperitoneum,after 0.5 hour and 2 hours under pneumoperitoneum and 0.5 hour after desufflation.Results Without pneumoperitoneum,different blood loss exerted different effect on arterial blood gas:RR,PaCO_2 increased(P

【Objective】To observe therapeutic effect of Guishen Pill(GP) combined with acupuncture(AP) for male immune infertility(MII) and to investigate influence on acid phosphatase(ACP) in seminal plasma.【Methods】One hundred and five MII patients were equally randomized into three groups.Group A received GP combined AP,group B received GP and group C received prednisone.The therapeutic effect was evaluated in the three groups after treatment,and the changes of ACP were observed before and after treatment.【Results】The total effective rate was 91.43%,88.57% and 68.57%,and the cure rate was 45.71%,22.86% and 11.43% in groups A,B and C respectively.The difference of total effective rate was significant in groups A and B as compared with that in group C(P

Objective To study the angiogenic effects of adenovirus mediated human vascular endothelial growth factor 165 and human angiopoietin-1 (Ad 5-VEGF 165 , Ad 5-Ang-1) in rat models of hindlimb ischemia. Methods Rat models of hindlimb ischemia were established by ligation and peeling off rat′s femoral arteries. Ad-VEGF 165 and Ad-Ang-1 were intramuscularly transferred into the rat ischemic hindlimbs. The expression of VEGF 165 and Ang-1 were examined by Western blotting. Immunohistochemistry was performed to illustrate the effects on rat ischemic muscles after gene transferring. Results (1) Western blotting showed a high expression of VEGF 165 and Ang-1 in the ischemic hindlimb muscle transferred with Ad 5-VEGF 165 and Ad 5-Ang-1 VEGF 165 respectively. (2) There was no significant difference between groups on 7 days after the transfer. The capillary to muscle fiber ratio was significantly higher in the treating groups than that in control groups and were significantly higher in VEGF 165 +Ang-1 group than in VEGF 165 or in Ang-1 groups on day 14th and day 21th. (3) Many structured vessels surrounded by a layer of smooth muscle cells were found in Ad-VEGF 165 and Ad-VEGF 165 +Ad-Ang-1 groups, the number of SMA + vessel per muscle fiber was obviously higher than those in control groups. (4) Enormous cells positive for BrdU appeared in treated muscles in Ang-1, VEGF 165 , VEGF 165 +Ang-1 groups and many of them were positive for C-Kit, an antigen expressed by pluripotent marrow stem cells. Some C-Kit+ cells were incorporated in sites of neovascularization. Conclusion (1) Vascular endothelial growth factor 165 and angiopoietin-1 can promote neovascularization in rat models of hindlimb ischemia and the angiogenic effect is much more significant in Ad-VEGF+Ad-Ang-1 group. (2) VEGF 165 can increase the number of vessles that are coated with smooth muscle cells which shapes are similar to arterioles. (3) Not only angiogenic effect but perhaps vasculogenic effct also contribute to the neovascularization.

Objective To investigate the effects and mechanisms of inositol hexaphosphate on diffe-rentiation of HT-29 human colon carcinoma cell line. Method Cells were exposed to various concentrations (control,1.8 mmol/L,3.3 mmol/L) of IP6 for different time (1d,3d,5d). Its effects on the cells were evaluated by detecting follow indices:(1) Observing ultrastructure of HT-29 cells by transmission electron microscopy. (2) Detecting alkaline phosphate (ALP) activity to evaluate cell differentiation. (3) Expression of c-Myc mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR). (4) Immunocytochemical stain was used to detect expression of Rb protein. Results (1) After having been treated at 3.3mmol/L concentration,the cell structure changed,and appeared tendency of differentiation,such as karyopyknosis,abundant cellular organs and microvillus increase. (2) The activity of alkaline phosphate increased along with the concentration and time. (3) RT-PCR showed that different concentrations of IP6 decreased the expression of c-Myc mRNA. (4) The immunocytochemical stain showed that different concentrations of IP6 increased expression of Rb protein. Conclusion IP6 can induce differentiation of HT-29 cell by augmenting expression of Rb,decreasing expression of c-Myc and blocking cell cycle.

Objective: To investigate the effects and mechanisms of inositol hexaphosphate(IP6)on cell cycle of HT-29 human colon carcinoma cell line. Method: HT-29 cells were exposed to various concentrations of IP6 for certain time. The effect of IP6 on cells proliferation was evaluated by MTT assay. Flow cytometric analysis was performed for cell cycle progression. Immunocytochemical stain was used to detect the expression of p53 and p21 protein. Results: A significant dose-dependent as well as time-dependent growth inhibition was observed in IP6-treated HT-29 cells, and associated with an increase in G1 arrest; Different concentrations of IP6 decreased the abnormal expressions of p53 gene and strongly increased the expression of p21. Conclusions: IP6 had an inhibitory effect on proliferation of HT-29 cells .and its mechanisms might be related to many factors, such as inhibiting the abnormal expression of p53 gene, inducing the expression of cell cycle inhibitor p21 which block the cell cycle.

Objective To investigate the CT findings of orbital inflammatory pseudotumor in different types and to improve the diagnosis and differential diagnosis of this disease.Methods CT findings of orbital inflammatory pseudotumor in 58 cases confirmed by clinic or pathology were analysed retrospectively.Results This disease could be categorized five types according to CT findings: ①mass type in 5 cases (8.6%), the characteristic CT findings were soft-tissue masses in intraorbit; ②diffusion type in 5 cases (8.6%) showed retrobulbar fat interspace of high-density without masses; ③myositis type in 22 cases(37.9%) showed the enlargement of entire muscles including the tendinous insertion;④lacrimal galnd type in 6 cases(10.3%) showed enlargement of lacrimal gland on CT; ⑤the mixed type in 20 cases (34.5%) showed hypertrophy of the extra-ocular muscles adjacent to mass and high-density of surrounding fat ,or hypertrophy of the extra-ocular muscles adjacent to enlarged lacrimal gland.There were in different degree of paranasal sinusitis such as maxillary sinusitis and/or ethmoid sinusitis in 22 cases(37.9%).Conclusion CT scan is of important role in diagnosing and differential diagnosing orbital inflammatory pseudotumor.