Adult ; Aspergillosis ; diagnosis ; etiology ; Diagnostic Errors ; Humans ; Lung Diseases, Fungal ; diagnosis ; etiology ; Male ; Silicosis ; diagnosis ; microbiology ; Silicotuberculosis ; diagnosis ; etiology ; Tuberculosis, Pulmonary ; diagnosis ; etiology

Objective To investigate the role of NFIC on the stimulation effects of cAMP-induced differentiation of stem cells from the apical papilla ( SCAPs) in vitro. Methods SCAPs isolated from dental papilla of human imma-ture third molars were cultured by enzyme digestion. SCAPs were transfected with lentivirus that overexpressed NF-IC gene ( ov-NFIC) or an empty vector ( LV-empty) and co-treatment with Forskolin. Mineralized nodule formation of each group was measured by alizarin red staining. Quantitative real-time reverse-transcription polymerase chain reaction was performed to test the expressions of RUNX2,ALP,OCN mRNA. Results Forskolin increased the ex-pression of Runx2, ALP, OCN mRNA as well as matrix mineralization in SCAPs, and the stimulation effects of For-skolin were enhanced by overexpressing NFIC gene. Conclusion The results indicate that NFIC can promote cAMP-induced differentiation of SCAPs.

Objective To observe the effect of rosuvastatin and memantine on EPC and learning or memory ability in VaD rats .Methods Forty adult male SD rats were randomly divided into sham operation group ,VaD model group ,memantine treatment group ,rosuvastatin treatment group , and memantine+rosuvastatin treatment group (combined treatment group) (8 in each group) .A VaD model was established by permanent ligation of bilateral common carotid arteries .The ani‐mals in rosuvastatin treatment group ,memantine treatment group and combined treatment group received gastric rosuvastatin 10 mg/(kg · d) ,memantine 10 mg/(kg · d) and memantine 10 mg/(kg · d)+rosuvastatin 10 mg/(kg · d) for 4 weeks while those in model group received gastric normal saline .Five weeks after operation ,the learning or memory ability was tested by Morris water maze test ,the escape latency and percentage of target quadrant were calculated ,the circulat‐ing EPC were detected by flow cytometry ,and the MVD in hippocampaus was assyed with vWF immunostaining .Results Five weeks after operation ,the learning or memory ability was signifi‐cantly lower in model group than in sham operation group (P< 0 .01) whereas the learning or memory ability ,the percentage of circulating EPC and the MVD in hippocampus were significantly higher in 3 treatment groups than in model group (P<0 .05) .Conclusion Rosuvastatin and me‐mantine can effectively improve the learning or memory ability of VaD rats by mobilizing their cir‐culating EPC and increasing the MVD in their hippocampus .However ,the effect of memantine or rosuvastatin does not differ greatly w hen they are used alone .

Objective To study the characteristics of calcific valvular heart disease (CVHD) with the aid of echocardiography. Methods Nine thousand and seventeen patients over 50 year-old who had received transthoracic echocardiographic examination were enrolled in the study. Diagnosis of CVHD was made with 2 dimension, M-mode and color Doppler echocardiography. General state of health, clinical manifestation and history of CVHD patients were also analyzed. Results Five hundred and seventy-four cases of CVHD were diagnosed and the characteristics of CVHD were observed. The diagnostic rate increased as the age increased. There was no significant difference between male and female. The calcified regions were commoner in aortic valves than in mitral valves, more frequent in non-coronary valves than in right or left coronary valves, and more frequently seen at tips of aortic valves and annuli of mitral valves than in other parts. The size of calcific plaques was usually between 3 and 10mm. Hemodynamic changes could be found in some patients. History of hypertension, coronary artery disease, and arteriosclerosis of the aorta were often found in these patients. Conclusions Echocardiography can be used to evaluate the position of calcification, size of calcification area, and degree of valvular stenosis or insufficiency, thus offers useful information for early diagnosis and treatment, and prognosis of CVHD in clinic.

Objective To explore the incidence and predisposing factors of coronary heart disease (CHD) among the patients with different types and extent of calcified valvular heart disease (CVHD). Methods 574 patients with CVHD diagnosed by echocardiography were divided into mild and severe groups based on the hemodynamic changes. The differences of incidence and predisposing factors of coronary heart disease between the two groups were compared. Results Ninety-seven cases of CVHD were categorized as having severe lesion, including aortic stenosis subgroup (AS group), aortic insufficiency subgroup (AI group) and mitral valve subgroup (MV group). All other patients were having mild lesions. There was no significant difference in blood pressure, blood lipid, fasting blood glucose, age and incidence of CAD, incidences in hypertension, hyperlipidemia and diabetes mellitus between two groups. Conclusions Mild CVHD showed similar prognostic value as that of severe CVHD, therefore it should not be ignored.

Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.

The article analyzes the problems of nurse-patient communication in nurse interns,and proposes the strategies of cultivating nurse-patient communication ability to reduce nurse-patient disputes and improve the overall quality of nursing students.

Objective To probe the effect of nursing interventions on depression of patients with acute stroke.Methods 72 cases of stroke patients with depression screened by Hamilton depression scale (HAMD)were divided into the intervention group and the control group with 36 cases in each group.The control group received routine treatment.The intervention group wag given nursing intervention based on routine treatment.The effect was assessed before and after 3,6 and 12 months of intervention with Zung self-rating depression scale(SDS)and improved Rankin scale(MRS). Results The value of SDS after 3,6 or 12 months was significantly lower than that of the control group(P＜0.05),and the value of MRS was significantly higher than that of the control group(P＜0.05).Conclusion Nursing intervention can reduce depression in patients with post-stroke depression.

Objective To study the effect of poly- DL- lactic acid (PDLLA) absorbable membrane on preventing adhesion after repair of peripheral nerve injury. Methods 60 SD rats were divided into 2 groups randomly: the suturing+ PDLLA group (experimental group) and the purely suturing group (contrast group), with 30 rats in each group. Their sciatic nerves were cut off and then sutured microsurgically. In the experimental group, the anastomotic stoma was encapsulated with PDLLA membrane; in the contrast group, there was no such a treatment. We conducted gross observations and histological examinations at 2, 4, 8, 12, and 16 weeks after operation, neural electrophysiological examinations at 12 and 16 weeks after operation, and image analysis by computer at 16 weeks after operation. Results The scar proliferation and adhesion near the anastomotic stoma in the experimental group were less extensive than those in the contrast group; regenerative nerve fibers grew more straight and orderly manner. Electrophysiological examinations and image analyses by computer also showed that the experimental group achieved better results than the contrast group. Conclusion Poly- DL- lactic acid (PDLLA) absorbable membrane can prevent scar proliferation and adhesion after repair of peripheral nerve and promote nerve functional recovery effectively.

Objective To explore the effects of vitrectomy on neovascular glaucoma combined with vitreous hemorrhage. Methods Seven eyes of 7 patients with neovascular glaucoma combined with vitreous hemorrhage underwent vitrectomy combined with phacoemulsification, panretinal photocoagulation, and trabeculectomy. The preoperative visual acuity ranged from light perception to 0.2, and the mean preoperative intraocular pressure was 54 mm Hg (38-64 mm Hg)(1 mm Hg=0.133 kPa). The mean follow-up was 8 months (6-15 months). Results The postoperative visual acuity ranged from light perception to 0.4, and the mean postoperative intraocular pressure was 17 mm Hg (10-30 mm Hg) which was significantly lower than preoperative one ( P