It was found that the Lao Shan polsaccharide P, one of the chemical components of polystictus versicolor increased the number of acetylated low density lipoprotein ( acLDL ) receptorsin the macrophages and stimulated the binding, internalization and degradation of (125I ) acLDL by the macrophages in vitro. The effect on cell degradation of ( 125I ) acLDL was dose-effect. At 100?g/ ml of the Lao Shan polysaccharide P, the effect was maximum and the degradation of ( 125I ) acLDL increased 77% ( P

BACKGROUND:The increase of serum cholesterol levels is positively correlated with the increasing incidence of osteoarthritis. Little is known about the role of high-fat diet in the degeneration of articular cartilage and induced arthritis.
OBJECTIVE:To observe the morphology change of the articular cartilage of New Zealand rabbits after high-fat diet, and explore the role of food in the articular cartilage degeneration.
METHODS:Forty New Zealand white rabbits were randomly divided into two groups. In control group, rabbits were fed with basal diet. In high-fat diet group, rabbits were fed with high-fat diet (20%lard and 80%basal diet). Fasting blood samples was taken every 4 weeks, to detect the triglyceride and total cholesterol levels. After rabbit were fed for 28 weeks, the knee joint was grossly observed and the femoral condyle cartilage was scanned by scanning electron microscope.
RESULTS AND CONCLUSION:Compared with the control group, the triglyceride and total cholesterol levels were significantly increased in the high fat group (P<0.05). Under scanning electron microscope, the high-fat group showed rough surface of condylar cartilage, shal ow pouch, messy arrangement, irregular shape and uneven size. The surface of cartilage exhibited focal denudation, irregular arrangement, uneven porosity and fractured cavity. Under higher magnification, smal mountain-like structure of cartilage surface became shal ow and flattened, the pores disappeared. Long-term high-fat diet may induce and aggravate cartilage damage, suggesting it may be involved in the pathogenesis of osteoarthritis.

The paper analyzed the compensation mechanism applied in public hospitals in different localities in China,which are currently experimenting with the zero price margin for drugs.It also introduced the revenue variations resulting from this practice.It is discovered that public hospitals can hardly make their ends meet with merely governmental financial input,and that lowered income of doctors and price distortion arc evident in those experimental hospitals,with little progress in overcoming the subsidization of medical services with drug sales.The authors recommended a comprehensive reform,the mechanism of diversified health input,change of internal incentive mechanism within the hospital,improvement in the healthcare service pricing mechanism,and exploration of new operation mechanisms for public hospitals.

Metastatic breast cancer (MBC) is a heterogeneous disease that has a variety of different clinical scenarios. There are few recognized therapeutic standards for MBC. Combining recent international guidelines and consensus recommendations with our clinical practice experience, the article will introduce and comment many sides about the treatment for MBC patients.

Objective To explore the clinical value of colposcopy directed biopsy in diagnosis of vaginal intraepitheal neoplasia (VaIN).Methods The clinical data of 106 VaIN patients diagnosed by colposcopy directed biopsy were reviewed and analyzed retrospectively.In the past medical history of 106 patients,46 patients had cervical cancer history,43 patients had cervical intraepithehal neoplasia history,13 patients had previous hysterectomy because of benign disease and 4 patients had uncertain past medical history.Results Among 106 patients,97 patients(91.51％,97/106) had abnormal pap smears of vagina,9 patients (8.49％,9/106) had abnormal pap smears of cervix.Histological analysis revealed that 69 patients were VaIN Ⅰ,24 patients were VaIN Ⅱ,13 patients were VaIN Ⅲ.The high-risk human papilloma virus (HPV) test at diagnosis were positive in 98 patients (92.45％,98/106),negative in 8 patients (7.55％,8/106).Eighty-seven patients VaIN were localized in the upper one third of the vagina,19 patients VaIN were localized in other parts of vaginal wall.Ninety-eight patients VaIN affected to the multifocal vaginal wall,8 patients VaIN affected to the focal vaginal wall.Conclusions It has highly therapeutic value of colposcopy directed biopsy in diagnosis of VaIN.But should closely correlated to past medical history,colposcopy should performed on the whole vagina wall and multiple punch biopsy to elevated positive rate.

Objective To compare biomechanical performances of parallel plate fixation and vertical plate fixation for better clinical treatment of humeral intercondylar comminuted fractures (HICF).Methods Models of T-shaped fracture were created using an osteotome at the distal end of humerus in 6 specimens of upper limb in 3 human adult cadavers.The 2 fracture models of left and right humeri in the same cadaver were respectively fixated with parallel plating and vertical plating.On a biomaterial-testing machine,a vertical load was applied onto the fixated distal humerus at a speed of 5 N/s till it reached 2000 N.The loading was repeated 3 times.Vertical compression displacement,intercondylar separating displacement,deformation of medial and lateral condyles and vertical compression stiffness were documented for statistic analyses. Results Under the same load,parallel plating led to significantly less compression displacement,separating displacement and deformation of condyles( P ＜ 0.05) but significantly greater axial compression stiffness( P ＜ 0.05) than vertical plating. Conclusion In biomechanics,parallel plate fixation has better performance than vertical plate fixation for HICF.

Objective To investigate the effect of ginsenoside-Rgl on paraurethral fascia fibroblastsmultiplication and the expression of proliferation cell nuclear antigen (PCNA) of stress urinary incontinence(SUI) women in vitro. Methods Specimens of human paraurethral fascia were obtained from 4 SUI womenduring tension-free vaginal tape (TVT) or tension-free vaginal tape-obturator (TVT-O) procedure.Fibroblasts were isolated and cultured by outgrowth technique. After reaching confluency fibroblasts weresubcultured every 5 days and cells after passage number 3 to 5 were used for assessment. The paraurethralfascia fibroblasts were treated with ginsenoside-Rgl at different concentrations (5, 10, 20 μmol/L) andfibroblnsts without Rgl were used as controL The multiplication conditions of paraurethral fascia fibroblastswere respectively detected by methyl thiazolyl tetrazolium (MTr) assay and the expression of PCNA byhistochemistry. Results ( 1 ) Compared with the control group, the growth rate of cells treated with differentconcentrations of Rgl after 72h [ (29±5 )%, (40±5 )%, (26±4)% respectively ] was significantly higher(P<0.01). (2)Compared with the control group, the stimulatory effect of Rgl on fibroblast growth wassignificant at 24 h (P<0.01), and peaked at 72 hi (29±5)% ,(40±5)%, (26±4)% respectively, P<0.01]. (3)Compared with the control group(28.77% ), there was a significant increase of PCNA-positivecells (P<0.01) after 48 h treatment with different concentrations of Rgl (49.24%, 83.48%, 54.50%respectively). Conclusion The results indicate that, at least in vitro, fibroblasts from paraurethral fasciataken from women suffering from SUI are able to proliferate after

Objective Find out the self-rated health of college students majoring in physical education and the influential factors, so as to offering some evidences which can help adopting adopt some measures to improve health condition. Methods Adopt method of random sampling to investigate the application ofamong 300 college students majoring physical education. Results The total condition of self-rated health of college students majoring physical education is good. The scores of self-rated physiology, psychology, social healthy sub-table are from 56. 6 to 95.00. While the lowest score is the self-rated of psychological health. Male students got higher scores than female in all aspects except the ability of daily life and social support and positive emotion. And the Differences in psychological health, physical symptom and function are significant (P＜0.05) .Comparison of college students self-rated health condition with other areas, it found that the students in Sichuan are in middle level of all healthy aspects. Conclusions The total condition of self-rated health about college students majoring physical education is good. More attention should put on to their psychology,besides regular diet, rest, and tired in physiology health.

Currently,there are a few problems existed in managing the research outlay of hospital including mistiness of the managing boundary,disfluency of the guarantee chain,weakness of the cost-benefit consciousness,irrationality of resource configuring,lack of supervision mechanism,etc.These problems could be resolved by strengthening the inner management of the outlay,carding the management mechanism,broadening the channel of outlay,concerning about the cost accounting,optimizing the resource configuring and founding the performance evaluation mechanism.To offer a powerful guarantee for the material effect of scientific research and to promote the continuous development of scientific research healthily,we should explore the new way of strengthening the management of the scientific research outlay incessantly.

OBJECTIVE:To investigate the effects of minocycline on clinical indexes,inflammatory factors and blood glucose of patients with moderate and severe chronic periodontitis complicated with type 2 diabetes(T2DM). METHODS:A total of 125 patients with moderate and severe chronic periodontitis complicated with T2DM selected from our hospital during Feb. 2013-Aug. 2015 were divided into control group(63 cases)and observation group(62 cases)according to random number table. Both groups were periodental non-surgical treatment as scaling and root planning. Control group was given iodine glycerin to fill periodontal pockets after surgery,2-4 times/d. Observation group was given Minocycline hydrochloride ointment,once a week,after surgery. Both groups were treated for 4 weeks. The levels of clinical indexes [probing depth,clinical attachment level(CAL),probing bleeding rate],serum inflammatory factors(CRP,TNF-α)and blood glucose(fasting blood glucose,glycosylated hemoglobin) were observed in 2 groups before treatment,3 months after treatment,and the occurrence of ADR was recorded. RESULTS:Be-fore treatment,there was no statistical significance in clinical indexes,serum inflammatory factors and blood glucose levels be-tween 2 groups(P>0.05). Three months after treatment,probing depth,probing bleeding rate and serum inflammatory factor lev-els of 2 groups were decreased significantly,and the observation group was significantly lower than the control group,with statisti-cal significance(P<0.05). Compared to before treatment,CAL of 2 groups were increased,without statistical significance before and after treatment or between 2 groups(P>0.05). The levels of fasting blood glucose in 2 groups were decreased,with statistical significance(P<0.05),but no statistical significance between 2 groups(P>0.05). Compared to before treatment,the level of glycosylated hemoglobin in control group was decreased,without statistical significance(P>0.05);the level of glycosylated hemo-globin in observation group was decreased significantly,and the observation group was significantly lower than the control group, with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between observation group (3.23%) and control group (0)(P>0.05). CONCLUSIONS:For moderate and severe chronic periodontitis complicated with T2DM,minocycline effectively improves clinical indexes of periodontitis,reduces serum inflammatory factors and blood glucose with good safety.