Objective To investigate the correlation of smokers in wnt/β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.Methods In the Department of Respiratory Medicine of the People's Hospital of Wuhan University,30 cases lung squamous cell carcinoma patients with a history of smoking,30 cases lung squamous cell carcinoma patients with no history of smoking,30 cases bronchiectasis patients with a history of smoking,30 cases bronchiectasis patients with no history of smoking were chose respectively as A group,B group,C group and D group.After the patients were treated by surgery,the removal of diseased tissue used immunohistochemical staining for CD68 + testing,TNF-α,wnt-2,β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method,then wnt-2,β-catenin,TCF-4 transcription of mRNA concentrations were detected by using RT-PCR method.Results Immunohistochemical studies showed that cells in TNF-α,CD68 + expression of A group,B group,C group and D group were respectively 0.895±0.036 and 0.931±0.237,0.125±0.018 and 0.631±0.064,0.083±0.042 and 0.311±0.062,0.053±0.004 and 0.196±0.020,the differences were statistically significant(P＜0.05).Westblot test Results showed that wnt-2,β-catenin,TCF-4 expression level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05),testing of RT-PCR showed that wnt-2,β-catenin,TCF-4 translation level showed a gradually decreasing trend,the difference was statistically significant(P＜0.05).Conclusion Smoking for body wnt/β-catenin pathway has a cell activation,after cigarette activate wnt/β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt/β-catenin signaling transduction circumstances.The detection of wnt/beta-catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.

Objective To explore the role of plasma level of homocysteine ( Hey ) in progressive ischemic stroke (PIS)of carotid artery system and to investigate the relationship between Hcy and carotid artery atherosclerosis(AS).Methods Plasma Hcy concentrations were measured in 68 patients with progressive ischemic stroke of carotid artery system (PIS group)and 72 patients with non-progressive ischemic stroke of carotid artery system ( non-PIS group ).Plasma Hcy level and carotid artery intima-media thickness ( IMT ) were compared among PIS group and non-PIS group as well as normal control group.The linear correlation between plasma Hcy level and IMT in PIS group was analyzed.Results Plasma Hcy concentrations was respectively (22.52 ± 5.26) μmol/L,( 16.38 ± 4.23) μmol/L and( 10.13 ± 2.16) μmol/L in PIS group,non-PIS group and normal control group( F =56.87,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant ( P ＜ 0.01 ).And there was also significant difference on plasma Hcy levels between PIS group and non-PIS group( P ＜ 0.01 ).The thickness of carotid artery IMT was respectively(1.12 ± 0.34)mm,(0.96 ± 0.27)mm and(0.43 ± 0.21 )mm in PIS group,non-PIS group and normal control group( F =28.47,P ＜ 0.01 ).Compared PIS group and non-PIS group with normal control group,the differences were statistically significant( P ＜ 0.01 ).And there was also significant difference on IMT between PIS group and non-PIS group ( P ＜ 0.01 ).In PIS group plasma Hcy level was positively correlated with the carotid artery IMT ( r =0.687,P ＜ 0.01 ).Conclusion The plasma Hcy level is closely related to the occurrence and development of carotid artery atherosclerosis and PIS of carotid artery system,detection of plasma Hcy level helps to predict the occurrence of PIS of carotid artery system and provide an evidence for the therapy decision.

Objective: To observe the effect of acupuncture around the diseased region combined with point injection on post-herpetic neuralgia. Methods: Point injection on 30 cases in the related segments of spinal cord after needling around the diseased region. Results: Twenty-seven cases got effect and the total effect rate was 90%. Conclusion: Acupuncture combined with point injection has remarkable curative effect on post-herpetic neuralgia.

Background and purpose:To investigate the clinico- pathological characteristics, prognosis and diagnosis of primary renal sarcoma that is a rare, highly malignant tumor with poor prognosis and survival. Methods:9 cases of renal sarcoma in our hospital were reviewed and analyzed. Nephrectomy was the main treatment for the patients. All the specimens were stained with histochemicial and immunohistochemical method, correlated literatures were also reviewed.Results:The distributions of pathology were malignant fibrous histiocytoma leiomyosarcoma, liposarcoma, myxosarcoma synoviosarcoma. Immunohistiochemical test was essential for the diagnosis of renal sarcoma.Conclusions:Pain and palpable mass were the common symptoms. Early radical surgery remains the optimal treatment, the role of chemotherapy and radiotherapy in the treatment modalities of the disease is still controversial.

Background and purpose:Fatty acid synthase(FAS)is a new tumor marker,the high expression of which has been reported to be associated with poor prognosis of cancer patients.This study investigated the expression of FAS in transitional cell carcinoma(TCC) of bladder and its clinical significance.Methods:The expressions of FAS were detected by streptavidin-peroxidase immunohistochemical method in the paraffin embedded sections of 65 TCC patients and the clinical data had been reviewed.15 normal bladder tissues were used as control group.Results:The positive rate of the expression of FAS in TCC was 58.5%(38/65),while only 2 of 15 cases in control group expressed FAS protein.The difference was statistically significant,the expression of FAS was related to the histological grades and recurrence but not different pathological stages.Survival analysis revealed that the 5-yr survival rate was lower in FAS positive patients than in negative patients(18% vs.44%,P

Objective To study the accuracy and efficiency of video colposcopy in diagnosis of cervical squamous intraepithelial lesions and clinical significance of seven colpocopic signs. Methods Two handred and senventy five patients with an abnormal cervical smear or vulva condyloma acuminata were re evaluated by video colposcopy. 265 cervical biopsies performed under the guidance of video colposcopy for pathological study, as well 8 endocervical curettage (ECC) and 237 loop electrosurgical excision procedure (LEEP). Results The sensitivity, specificity and positive rate of video colposcopy in evaluation of cervical squamous intraepithelial lesions were 95 9%, 77 7%, and 84 5%. Video colposcopy diagnostic accuracy was 93 8% tally with the pathological results in observing normal transformation zone. False negative rate was 6 1%. 76 2% of cases with subclinical papillomavirus infection by acetowhite epitheliun, while false negative rate was 23 7%. Twin signs, triple signs and cervical carcinoma were 100 0% tallying with the diagnosis of cervical lesions. Conclusions Video colposcopy can be a very accurate diagnostic method in clinical practice. It is quite sensitive in diagnosis of severe cervical lesions. However, there was a relative high false negative rate in diagnosing mild cervical lesions.

0.05)and the total costs of the 3 groups were 1 111.82 yuan,1 132.82 yuan and 1 219.62 yuan,respectively.CONCLUSION:Group A(Ceftriaxone Sodium)has been proved to be preferable for children's bronchopneumonia.

Construction of teacher's professional morality is a major issue that matters the development of both teachers themselves and medical colleges,especially under the current circumstances of constructing a harmonious society.This article summarizes the phenomenon of absence in teacher's professional morality in market-oriented economy,elaborates theoretically the necessity of strengthening teacher's professional morality construction in medical colleges,and proposes briefly strategies to strengthen the construction of teacher's professional morality.

Objective To investigate the effect of obesity on the early complications after esophageal cancer operation.Methods Clinical data of 327 patients who had esophageal cancer operation in our hospital from August 2012 to September 2015 were retrospectively analyzed.The patients were divided into 4 groups:lean group(n =75), normal group(n =120),overweight group(n =103)and obesity group(n =29)by body mass index(BMI).The impact of obesity on early complications of esophageal carcinoma after surgery was analyzed.Results The operation time of the lean group,normal group,overweight group and obesity group were (257.8 ±52.6 )min,(289.3 ± 48.7)min,(321.5 ±55.9)min,(367.9 ±50.2)min respectively.The operation time was increased with the increase of BMI (F =13.397,P =0.000).The incidence rates of incision infection,empyema,hoarseness,diaphragmatic hernia,postoperative bleeding,chylous fistula,pulmonary complication were 5.3%,1.3%,1.3%,1.3%,2.7%, 2.7%,16.0% in the lean group,5.0%,1.6%,1.6%,1.3%,1.6%,1.6%,17.5% in the normal group,5.8%, 1.9%,0.9%,1.9%,1.9%,1.9%,1.9% in the overweight group,10.3%,3.4%,0.0%,3.4%,3.4%,0.0%, 13.8% in the obesity group.No significant differences were found about the hospital days,wound infection,empyema, hoarseness,diaphragmatic hernia,operation hemorrhage,chylous fistula,pulmonary complications among the 4 groups (all P >0.05 ).The incidence rates of cardiovascular complications and anastomotic leakage after operation were 0.0%,4.0% in the lean group,1.9%,5.0% in the normal group,6.8%,9.7% in the overweight group,13.8%, 17.2% in the obesity group.The incidence of cardiovascular complications,postoperative anastomotic leakage increased with the increase of BMI.The differences of the four groups were statistically significant(F =1.675,1.054, all P <0.05).Conclusion Obesity can increase the incidence of early postoperative complications in patients with esophageal cancer.But obesity is not a contraindication of surgery for esophageal cancer,but obese patients should be more carefully treated in the perioperative period.

Objective To compare the clinical effect of erythromycin and azithromycin in the treatment of mycoplasma pneumonia.Methods 120 children with mycoplasma pneumonia were randomly divided into study group and control group according to digital table method,60 cases in each group.The control group was treated with erythro-mycin,while the study group used azithromycin treatment.Clinical curative effect was compared between the two groups.Results The disappearing time of cough[(4.58 ±0.75 )d],fever[(4.75 ±1.74)d],pulmonary rales [(7.84 ±2.85)d]of the study group were significantly shorter than those in the control group[(4.58 ±0.75)d, (4.75 ±1.74)d,(7.84 ±2.85)d],and the differences were statistically significant (t =7.54,385,5.93,all P <0.05).The total effective rate in the study group (95.00%)was significantly higher than that in the control group (83.33%),and the difference was statistically significant (χ2 =8.08,P <0.05).There was no significant difference in the incidence of adverse reactions between the study group and the control group (χ2 =0.64,P >0.05 ). Conclusion Mycoplasma pneumonia in children for early diagnosis and treatment of dry,use of azithromycin curative effect was better than that of erythromycin.It could effectively relieve patients of fever,and alleviate the clinical symptoms of cough,pulmonary rales.