Objective To investigate the status of internet addiction among adolescent students of Tianjin and analyze its related factors.Methods Stratification cluster random sampling method was used in this questionnaire survey among nine schools of Tianjin.The data was analyzed by logistic regression with SPSS11.5.Results The results showed that the Family harmony(OR=0.433),good personal education achievement(0.645),and sports participation(0.705) were the protective factors,while the Family member accident(OR=1.735) was the risk factor for IAD in adolescent students.Good Family economic conditions(OR=0.793),Family harmony(0.778),good personal education achievement(0.787),and sports participation(0.750) were the protective factors,while subsidization(OR=1.404) and family member accident(1.244) were the risk factors for IAT in adolescent students.Conclusion Family has close relationship with the formation of adolescents' IAD.Besides,personal education achievement and sports participation also have good influence on the formation of IAD.

Objective To evaluate the relationship between Internet addiction disorder(IAD),Internet addiction tendency(IAT) and behavior type A of young students aged ≥16 years in urban districts,Tianjin.Based on the result,the intervention resolution suggestion was put forward.Methods 4 484students(male2 331,female2 153) anonymously completed the Questionnaire on Situation of Internet Using of Students compiled for this study and the Questionnaire on types of Northern Chinese adult's Behavior.All data was recorded with the evaluating system for adolescent Internet addiction.Result There are 306(6.82%) cases of IAD and 600(13.83%) of IAT among the students.The screened positive rate in boys was 9.87% for IAD and 15.66% for IAT,which significantly higher than those in girls(3.53%,10.92%).The rates in students with behavior type A and type M were the same but significantly higher than that in students with behavior type B.After weighing cases by power on learning stage and sex,the results showed the prevalent rates of IAD and IAT in students with behavior type A and type sub-A were significantly higher than those in students with behavior type M,type sub-B and type B.After weighing cases by power only on learning stage,the positive rates of IAD were higher in students with behavior type A(8.87% in high school students,11.11% in college students) than those in students with type M(7.77%,7.81%) and type B(4.35%,4.44%).Conclusions The results show a significant relationship between IA and behavior type A in students.It should be strengthened to take psychological intervention for young students,especially for students with high risk of IA and IAT in senior high schools.

Objective To know the prevalence and distribution of Internet Addiction Disorder(IAD) among the adolescent students in urban districts in Tianjin.Methods This survey was carried out during November,2006 to April,2007 with Stratified cluster random sampling method.8225 questionnaires were valid among 8694 participants.Xing Yi's IAD diagnosis standard was used on the survey.Internet Addiction Tendency(IAT)by our definition was those students who had 3 or 4 of 10 indicators.The national standard population proportion of school students from junior high school to college in 2000 were used in weighing cases by power.Results 1.The detection rates of IAD between students online 2,3 or 4 hours per day were significantly different of the check out rate of IAD from the students on-line 2,3,or 4 hours per day,except time factor in the indicators;2.The detection rates of IAD between students with different learning stage or education system were also significantly different;the highest rate was among the students at special technical secondary school(11.69%),then followed by technical college and college students(6.74% and 6.73%),and junior high school and senior high school students(5.98% and 5.95%).The detection rates of IAT were increased by learning stage;the lowest rate was 8.65% in junior high school students,followed by 12.11% in high school students,13.96% in technical secondary school students,15.58% in technical college students and 16.73% in college students;3.After weighing case by power on learning stage or education system,the total IAD prevalence was 6.90%,9.50% for boys and 4.17% for girls.Total IAT prevalence was 11.78%,14.43% for boys and 8.96% for girls;4.For students in area with higher economic level,the rates of IAD and IAT were 4.16% and 8.27%,respectively,lower than those in other economic levels;5.Both IAD and IAT prevalent rates were no significant difference between the key high schools and common high schools.Conclusion The students of special technical secondary schools are high risk population for IAD and IAT.Students in area with higher level of economic have lower rates of IAD and IAT.It is suggested that it is needed to carry out health education on Internet using among the adolescent students together with family,school and social good environment.

Programmed death-ligand 1 (PD-L1 ),which is highly expressed in NSCLC,can be divided into two categories:membrane PD-L1 and soluble PD-L1 .PD-L1 participates in tumor immune escape through combining with its receptor.PD-L1 immune checkpoint inhibitors have entered the phase Ⅰ studies and showed a good application prospect.It is also found that the sensitivity of PD-L1 immune checkpoint inhibitors is strongly associated with the expression of PD-L1 in tumor.Therefore,PD-L1 can be used as a biomarker to pre-dict its curative effect.

Objective To explore the effects of early acupuncture on the expression of neurofilament protein (NFP) in the brain and the neurobehavior of the neonatal rats with brain injury. Methods The pregnant Wistar rats were randomly divided into two groups: lipopolysaccharide (LPS) group and control group, which were consecutively injected intraperitoneally with LPS or saline. The full-term newborn rats from LPS group were randomly divided into acupuncture group and model group. Acupuncture group was acupunctured from postnatal 8 d. They were evaluated with hanging test, and the expression of NFP was detected with immunohistochemical stain 21 d after birth. Results Both the scores of hanging test and the expression of NFP were the highest in the control group, the lowest in the model group and medium in the acupuncture group (P<0.01). Conclusion Acupuncture may increase the expression of NFP and improve locomotor function of the neonatal rats with brain injury caused by intrauterine infection.

BACKGROUND:The type B synoviocytes induced by transforming growth factor β1(TGF-β1) have the potential to differentiate into chondrocyte,which can remain the phenotype in vitro. However,whether the transfected cell combined with scaffold can form cartilage tissues need further research. OBJECTIVE:Rabbit type B synoviocytes was transfected by liposome method in vitro,combined the cells with Pluronic-F127,and then implanted into nude mouse to construct tissue engineering cartilage. Additionally,to explore the feasibility of synoviocytes differentiate into chondrocytes.DESIGN,TIME AND SETTING:The randomized animal experiment of cytology observation. The experiment was performed at the Medical Research Center,the Second Affiliated Hospital of Sun Yat-sen University between April 2007 and May 2008.MATERIALS:Healthy New Zealand white rabbits,aged 3 months,and 12 BALB/c nude mice,aged 4 weeks,weighted 20 g.METHODS:The synovial membrane tissues were taken out from the rabbit knee,isolated by enzyme digestion,and then transfected. The positive cloning was screened by G418,and the expression of TGF-β1 and collagen Ⅱ was detected. The Pluronic-F127 was dissolved at 4℃ and prepared fluid with concentration of 30%. The fluid was mixed with cells. At the same time,there were 2 groups as the control group:chondrocytes with Pluronic-F127,and synoviocytes transfeced by pcDNA3.1(+)and Pluronic-F127. The cell density was 5x1010/L. Each compound (0.2 mL) was injected into the subcutaneous of the nude mouse back. Rats were sacrificed at weeks 4,6 and 8 to harvest samples.MAIN OUTCOME MEASURES:Cell growth curve;phenotype change of the cells after transfection;histological observation of the tissue in the subcutaneous of the nude mouse back. RESULTS:Cell growth curve demonstrated that the living activity of the transfected cells was temporary decreased,which returned into normal level at days 6,7 after transfection. At day 4 after transfection,TGF β1 were positive expressed,and at day 7,the collagen Ⅱ staining were positive. The compounds in the subcutaneous of the nude mouse back formed immature chondroid tissues at week 4,which turned to mature chondroid tissue at week 8,and the collagen Ⅱ staining were positive.CONCLUSION:The transfected synoviocytes can express the phenotype of chondrocytes in vitro,and form chondrocyte-likecells. The transfected synoviocytes with Pluronic-F127 can form chondroid tissues in nude mice.

AIM: To investigate the effect and mechanism of Jingui Shenqi Pill(JGSQ) on pulmonary fibrosis. METHODS: Pulmonary fibrosis was induced by bleomycinA5 in SD rats.Rats in drug group were killed after being treated by taking JGSQ daily for 28 days.The lungs of all rats were harvested for histopathological studies.The contents of tumor necrosis factor ?(TNF-?)in lungs were measured and compared. RESULTS: The degree of the alveolitis and pulmonary fibrosis, the contents of TNF-? in lungs increased significantly in the model group as compared with the normal group and the JGSQ treated group(P

To introduce the methods and experience of arthroscopy treating different types of sports traumatic ankle arthritis.Methods The study involved 25 patents with spots traumatic ankle arthritis treated under ankle arthroscopy from January 2008 to October 2010.American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scoring system was applied to make functional evaluation before and after operation.Also,anterior drawer stress radiographs were used to assess the stability of joint.All patients had arthroscopic examination and received corresponding treatments,including synovial scar tissue resection,cartilage surface trimming,and microfracture management.Five patients underwent stage Ⅰ extramalleolus ligament repair.Results Arthroscopy showed simple synovitis in four patients,impingement syndrome developed from synovitis with fibrous scar tissue formation in 10,tibial or talus cartilage lesions in 11 and congestion of fresh injury of fibular end in four.A total of 23 cases were followed up for average 13.5 months (range,6-24 months).The ankle-hindfoot score was increased from preoperative (54.3 ± 6.2) points to (81.5 ± 7.9) points at 1 month,(82.9 ± 2.5) points at 3 months,( 83.1 + 2.1 ) points at 6 months and ( 83.5 ± 3.9 ) points at 12 months,with statistical differences ( t =13.01,20.58,21.10,19.11 respectively,P＜0.05).The anterior drawer stress radiographs showed that the anterior translation of talus improved from preoperative ( 15.2 ± 2.5 ) mm to postoperative (3.5 ± 0.2 ) mm,with statistical difference ( t =9.33,P ＜ 0.05 ).The anterior drawer test and talus tilt test were both negative for all patients.Concluslons Ankle arthroscopy is characterized by micro trauma and fast recovery.Symptomatic treatment under arthroscopy can obviously relieve the uncomfortable symptoms following sports traumatic ankle arthritis.

Objective To evaluate the mid-term results of arthroscopic anterior cruciate ligament (ACL) reconstruction using Transfix technique.Methods From August 2002 to December 2003,38 patients (24 left knees and 14 right knees) with ACL ruptures underwent arthroscopic ACL reconstruction with hamstring tendon using Transfix femoral fixation and interference screws tibial fixation.There were 21 males and 17 females,aged from 19 to 48 years (average,28.4 years).The causes of injury included sports injury in 27 cases,traffic injury in 2 cases and fall injury in 2 cases,and 7 patients had no significant injury.There were 6 cases of acute injury and 32 cases of old injury.Anterior drawer test was positive in 35 cases,weak positive in 1 case and negative in 2 cases.Lachman test was positive in 37 cases,and weak positive in 1 case.The Lysholm score was used to evaluate the results.Moreover,X-rays and MRIs were used to observe the conditions of graft and bone tunnel.Results Thirty-six patients were followed up for 6.3 to 7.6 years (average,6.8 years).All patients had normal joint range of motion.The Lysholm score improved from preoperative 64.4±4.52 to postoperative 85.6±4.60,and the difference was statistically significant (P ＜ 0.05).The X-rays and MRIs showed femoral and tibial tunnel enlargement in 3 cases,femoral tunnel enlargement in 5 cases and tibial tunnel enlargement in 3 cases.No joint space narrowing was found in all patients.One patient underwent arthroscopic revision ACL reconstruction with allograft tendon using bioabsorbable interference screws fixation on both tibial and femoral sides for re-rupture of ACL 4 years after operation due to trauma.Conclusion Arthroscopic ACL reconstruction with hamstring tendon using Transfix femoral fixation and interference screws tibial fixation is an effective method,which can provide satisfactory joint range of motion and stability.

Objective To evaluate the curative effect of comprehensive rehabilitative treatment for lumbar intervertebral disc protrusion by using the MRI tests before and after the treatment. Methods Forty patients with lumbar intervertebral disc protrusion were treated by a comprehensive rehabilitative treatment program composed of physical modalities, medications, traction, massage and medical gymnastics, for 30 days. MRI test were performed with all the patients, and comparison of the MRI manifestations before and after the treatment was conducted to evaluate the therapeutic efficacy. Results The clinical effective rate was 82.5%. There was no significant difference between the MRI manifestations, including MRI signal intensity, thickness and degree of the protrusion, anteroposterior and transverse diameter, width of lateral recess, before and after the treatment. Conclusion The comprehensive rehabilitative treatment program used in this study did not significantly decrease the size of lumbar intervertebral disc protrusion, but did relieve or cure the clinical symptoms of the patients.