Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; surgery ; Humans ; Immunohistochemistry ; Keratins ; metabolism ; Ki-67 Antigen ; metabolism ; Male ; Pancreas ; metabolism ; pathology ; Pancreatic Neoplasms ; metabolism ; pathology ; surgery ; Pancreaticoduodenectomy

The arbuscular mycorrhizal(AM)symbiosis is a mutualistic endosymbiosis formed by plant roots and AM fungi.The AM symbiosis is manifested in bidirectional nutrient exchange: AM fungi obtain carbon from their plant host while assisting the plant with the acquisition of mineral nutritions(in particular phosphate)from the soil.AM symbiosis facilitate phosphate uptake is the central reason of plant growth and development.Phosphate transport into the root is mediated by phosphate transporters,and phosphate transporters play a role in the acquisition of phosphate released by the fungus in the AM symbiosis.The molecular biology of arbuscular mycorrhizal symbiotic phosphate transporters in plants was summarized.AM phosphate transporter belong to Pht1 family,is not only essential for the acquisition of phosphate,also critical to AM symbiosis.Study of phosphate transport roles and their gene regulation will further our knowledge of the interaction between the two symbiotic partners,and so as to provide innovative approach to improving phosphate efficiency and agricultural yield.

OBJECTIVE:To establish a method for the determination of Oleanolic acid and Ursolic acid isomers in Hedyotis corymbosa.METHODS: Capillary zone electrophoresis with high frequency conductivity detection was adopted.The separation of sample was performed on an uncoated fused silica capillary(55 cm?75 ?m ID) at an effective length of 50 cm.1.2 mmol?L-1 Triethylamine(TEA)-HCl(pH10.0) buffer containing 0.24 mmol?L-1 ?-cyclodextrin was selected for the running buffer solution.The voltage applied was 12.5 kV and the sample was injected by gravity for 10 s at a height of 20 cm.Brufen was used as internal standard.RESULTS: The linear ranges for Oleanolic acid and Ursolic acid were 3.9～39.0 ?g?mL-1(r=0.999 1) and 20.0～140.0 ?g?mL-1(r=0.999 0),respectively,and their average recovery rates were 96.5% and 96.0% respectively and RSD were 1.98% and 1.09%,respectively(n=6).CONCLUSION:It was proved that the method was simple,accurate,rapid and reproducible,and applicable for the quality control of Hedyotis corymbosa.

Objective To know the influence of comprehensive nursing intervention on the prognosis of elderly patients with chronic heart failure. Methods By the prospective study method, 76 cases of elderly patients with chronic heart failure were selected from January 2014 to July 2015 as the research objects. According to the different methods of hospital care, they were divided into the observation group and control group, patients in the observation group had accepted the comprehensive nursing intervention measures, while the patients in the control group adopted conventional nursing intervention measures. Compared the nursing effect, anxiety scale (SAS), depression (SDS), left ventricular ejection fraction (LVEF), 6-minute walking distance and heart failure in the quality of life scores between the two groups. Results After 3 months of comprehensive nursing intervention, the rate of grasp nursing knowledge, compliance, nursing satisfaction and readmission in the observation group was 78.95%(30/38), 81.58%(31/38), 92.11%(35/38), 31.58%(12/38) respectively, which was significant better than that of in the control group 57.89%(22/38), 65.79%(25/38), 57.89%(22/38), 52.63%(20/38),respectively, the differences were statistically significant (χ2=3.897 4-7.916 7, P<0.05). The score of SAS, SDS, LVEF and the length of the 6-minute test in the observation group was 41.6±3.9, 45.3±5.2, (52.1±5.5)%, (568.3± 44.2) m, which was significant better than that of in the control group, 46.8 ± 4.7, 51.6 ± 4.9, (46.8 ± 4.6)%, (462.3 ± 46.5) m, t=4.556 6-10.185 1, P<0.05. Conclusions Comprehensive nursing intervention can effectivey improve the prognosis of patients with chronic heart failure, slow the progression of disease, and then strength the confidence of fighting disease.

Recent developments on separation and analysis of bacterial cells by capillary electrophoresis are reviewed. Based on bacterial surface characterization, capillary electrophoresis technique can be utilized for rapidly identify and quantify intact bacteria and microbes at a certain phase. The microbe-molecule interactions can also be described. The separation of microbes that can not be cultured in lab appears to be possible by this method. It makes profound effects on separation science and microbiology to establish capillary electrophoresis procedure for the separation and analysis of bacterial cells.

[Objective] To observe the therapeutic effect of wind - dispelling, blood - activating and stasis - removing Chinese herbs combined with autoepidermic grafting for vitiligo. [Methods] Forty cases of vitiligo were randomized into two groups: 28 cases in Group A were treated with Chinese herbs combined with autoepidermic grafting (235 grafts in all) and 12 in Group B with autoepidermic grafting (118 grafts in all) alone. [Results] In Group A, 1 case was cured, 13 were markedly effective, 10 effective and 4 ineffective and 0, 3, 3, and 6 in Group B respectively. The therapeutic effect in Group A was superior to that in Group B (P = 0.034). [Conclusion] Chinese herbs combined with autoepidermic grafting has a better effect than autoepidermic grafting alone for vitiligo.

Objective:To investigate the effect of porcelain bake cycles on oxide layer form ed on cobalt-chromium alloy surface.Methods:Fixed spot analysis of EPMA methods was adopted to measure the oxygen at o mic percentage inward from the edge of cross-section interface of alloy pieces. The adherent state of oxide layer on the surface of substrate alloy was observed .Results:The oxide layer on the surface of alloy had stable thickness and contain ed more and more oxide as porcelain firing times increased.In some area,oxide la yer fractured from the substrate metal.Conclusion:Cobalt-chromium alloy frame should be abrased and polished thoroughl y after porcelain bake cycles.

Objective To measure the magnitude of galvanic currents produced by different occluding metallic contact.Methods The circuits of instantly different metallic contact were simulated in artificial saliva,and electrical potential of every couple and 15 currents of instant contact were measured.To simulate night sleep,after 8 h soaking,15 currents were obtained in addition.Results During early soaking,gold/Co-Cr alloy produced the highest current(6.17 ?A),while there were significant differences in currents between gold/zinc-free amalgam,gold /zinc-containing amalgam and other couples(P0.05),and the current value was low all the time.Potential of zinc-containing amalgam was more negative than other alloys,and it was always under anodic behavior during the experiment and was corrupted acceleratedly.Conclusion Couples of gold/titatium,gold/zinc-containing amalgam,zinc-free/zinc-containing amalgam appearing in oral will do harm to the patients′health,while Co-Cr/zinc-free amalgam can coexist in oral.Corrosion behavior of amalgam is affected by different contents of zinc.

The incretion secretion metrical technique is the important embranchment in clinical diagnosis presently,in which the magnetic separate enzyme immunoassay is the main technique.This paper introduces the principle and design of Chinese enzyme immunoassay instrument,and emphatically discusses the design of nonlinear data processing model.

Objective:To explore the dynamic changes of nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats with seawater immersion injury after open abdominal trauma Methods:Thirty-two Sprage-Dawley rats were randomly assigned into four groups:normal control group,celiac seawater-immersing trauma stress 1,2,3h groups.The contents of nitric oxide and prostaglandine in gastric mucosa and plasma of rats were detected by chemochromometric and immunoassay methods respectively.Results:Compared with normal control group,both the nitric oxide and prostaglandine levels in gastric mucosa and plasma of rats under celiac seawater-immersing trauma were progressively increased (P