Objective To evaluate performance of the AVE‐766 automated urine sediment analyzer(AVE‐766) based on the ma‐chine vision for detecting erythrocytes(RBCs) ,leukocytes(WBCs) ,epithelial cells(ECs)and CASTs in urine specimen .Methods The within‐run variable coefficients(CVs) ,linearities and carryover rates for RBC ,WBC ,EC ,and CAST in urine specimens detected by using the AVE‐766 were analyzed ,the results of RBC ,WBC ,EC ,and CAST count in urine specimens detected by using AVE‐766 and Fast‐Read102 counting plate(Fast‐Read102) were compared .Results The within‐run CVs for RBC ,WBC ,EC ,and CAST detected by using AVE‐766 and Fast‐Read102 were increased by decreases of concentration of urine sediment .Good linearities (R2 >0 .97 ,P<0 .05) were observed for RBC(in the range of 60 -1 255/μL) ,WBC(in the range of 68 -2 718/μL) ,EC(in the range of 28-296/μL) and CAST(in the range of 5-86/μL) detected by using the AVE‐766 .The carryover rates for RBC ,WBC , EC ,and CAST detected by using AVE‐766 was 0 .9% or less .The values detected by using AVE‐766 were correlated well with those detected by using Fast‐Read102 for RBC ,WBC ,and EC in urine specimens(0 .67< r<0 .75) .However ,for CAST ,the values detected by using AVE‐766 were poorly correlated with those detected by using Fast‐Read102(r=0 .183) .There were statistically significant differences between manual and automated urinalysis for RBC ,WBC ,EC ,and CAST in urine specimens(P<0 .05) .Con‐clusion The AVE‐766 could not take over microscopic examination ,only is suitable for the first screening to detect RBC ,WBC , EC ,and CAST in urine specimen .

Objective To explore the imaging diagnostic value of gastrointestinal stromal tumor(GIST).Methods The retrospective study on GIST proved by operation and pathology in 11 cases was done.There were pneumobarium double contrast examination in 10 cases,CT scanning in 4 cases,superior mesenteric ateriography in 2 cases and fiberoptic endoscopy in 7 cases.Results The locations of tumors at gastric were 7 cases,intestinal 2,lipper and row oseophagus to gastric cardiac in 1,respectively.The detective rate of GIST was 100%,75%,100% and 100% respectively by pneumobarium double contrast examination,CT,arteriography and fiberoptic endoscopy.Conclusion Pneumobarium double contrast examination and fiberoptic endoscopy are important methods for diagnosis of GIST.CT and arteriography can better show the location of tumors and differential diagnosis of benign and malignant of tumor.

BackgroundBigbag intraocular lens (IOL),due to its special conducive design to support vitreous and retina after cataract surgery in high myopia,and ensure the visualization of peripheral retinal,is closely concerned.But whether its concave design has the advantage in reducing aberrations on the basis of stability or not is worth to be studied.ObjectiveThis study was to evaluate the high order aberrations(HOA) of phacoemulsification and the Bigbag IOL implantation in patients with myopia and cataract.MethodsA retrospective case-observational study was designed.Total 39 eyes of 39 cases with cataract and high myopia were enrolled in this study.The patients were divided into Bigbag IOL group and AR40e IOL group.Phaco-chop technique and IOL implantation were performed in April to June,2010 in Tianjin Eye Hospital.Two months after surgery,the operated eyes were clinical examined and the aberrations were detected including Root-Mean-Square (RMS),coma,spherical and Trefoil.Postoperative RMS values of HOA components of the whole and interior optic with iTrace were compared.Results The operation was smoothly with the correct IOL position after two months.No complication was found.Under the 4.5 mm pupil diameters,the whole ocular HOA RMS values and coma were significantly different between Bigbag IOL group and AR40e IOL group( t =-3.296 、-3.322,P＜0.05 ),but no significant differences were seen in spherical and Trefoil aberrations ( t =- 1.256、- 0.573,P＞ 0.05 ).Regarding to the interior optic with iTrace aberration,only the coma showed the significant difference between Bigbag IOL group and A R40e IOL group( t =-2.004,P＜0.05 ),and there were no significant differences in RMS,spherical and Trefoil aberrations( t=-1.073、0.380、0.288,P＞0.05 ).ConclusionsThe Bigbag IOL,a design for high myopia,is safe and effective after implant for the high myopia with cataract.It offers more exact obligate degree and better visual quality.The chief total and interior HOA components difference between groups is coma aberration.

Background Aberrant proliferation of residual lens epithelial cells (LECs) is one of main causes of posterior capsular opacification (PCO).Researches indicated that Wnt3a signaling pathway promote proliferation of epithelial cells,but its effect on LECs is still unclear. Objective The present study was to investigate the effects of Wnt3a on proliferation of human LECs and its mechanism and to provide a new gene target in the prevention and treatment of PCO. Methods Human LECs line (SRA01/04 cells ) was cultured and then incubated to 6-well plate at the density of 4×105/well.A human Wnt3a cDNA expressing vector targeted human LECs was constructed to increase the Wnt3a expression in SRA01/04 cells,and pcDNA3-HA expression vector was used as the control group.The expression of Wnt3a was identified by Western blot assay after transfected.The growth and proliferation of SRA01/04 cells were detected by MTT and flow cytometry (FCM).The expressions of β-catenin,cyclin D1 and c-myc in the cells were detected by Western blot assay.β-Catenin expression was localized using immunofluorescence assay,and the expression and localization of proliferating cell nuclear antigen ( PCNA ) were analyzed by immunocytochemistry for the exploration of the active mechanism of Wnt3a to proliferation of LECs.Results Human Wnt3a cDNA expression vector was designed successfully and transiently transfected to SRA01/04 cells,and Wnt3a/SRA01/04 cells and pcDNA3-HA/SRA01/04 cells were obtained.The expression of Wnt3a was verified in the Wnt3a transfected group compared with the control group.MTT indicated that the cell proliferating rate was significantly different between the Wnt3a transfected group and the control group ( Fgroup =15.235,P =0.005 ;Ftime =369.677,P =0.000),and that in various time points after transfected was significantly different (t =20.843,P=0.001 ;t =26.214,P＜0.001 ;t=25.177,P=0.001 ;t =35.516,P＜0.001 ;t =615.056,P＜0.001 ).The proportion of SRA01/04 cells in G1 phase was 51.74％ in the Wnt3a cDNA transfected group,with a significantly decrease in comparison with 79.44％ of the control group.However,the proportion of SRA01/04 cells in S phase in the Wnt3a cDNA transfected group was higher than that of the control group (36.23％ versus 12.34％ ).The positive expression rate of PCNA protein in SRA01/04 was (47.00％ ±7.58％ ) in the Wnt3a cDNA transfected group and ( 16.00％ ±3.61％ ) in the control group with a significant difference between them (t =8.256,P＜0.01 ).After 48 hours of transfection of the Wnt3a cDNA,the expression amount of β-catenin proteins was higher and the immunofluorescence was stronger in cell nucleus,and the expressions of cyclin D1 and c-myc proteins were elevated in Wnt3a/SRA01/04 cells. Conclusions The overexpression of Wnt3a activates the Wnt/β-catenin signaling pathway and downregulates the expression of a subset of target genes,including cyclin D1 and c-myc,which plays an important role in promoting the proliferation of human LECs.

Background The test for visual acuity is the conventional standard for evaluating visual quality.However,there is little correlation between visual acuity and visual disability.Therefore,other clinical observations such as contrast sensitivity or straylight have been used in the clinical assessment of visual quality after cataract surgery.Objective This study was to examine the change in the amount of straylight in cataractous eyes and to evaluate the correlation of the types of cataract with visual quality.Methods A non-randomized case-controlled study was designed.Eighty eyes of 40 patients with age-related cataract were enrolled in this study,including 22 eyes with hard nucleus cataract,19 eyes with cortical cataract,23 eyes with mixed cataract and 16 eyes with posterior subcapsular cataract diagnosed based on the criteria from Lens Opacities Classification System Ⅲ(LOCS Ⅲ).The values for straylight and best corrected visual acuity (BCVA) were measured with the C-quant straylight meter and Snellen chart,respectively.The differences in the straylight values among the different types of cataract were analyzed,and the correlation of the straylight value with age or BCVA was assessed.Forty eyes of 40 age-matched normal people served as controls.Results Examination was completed in sixty-two eyes of 31 patients in the cataract group at a completion rate of 77.5％,and all the subjects in the control group finished the examination at a rate of 100％.The mean straylight value was (2.06±0.88) log in the cataract group and (1.96±0.42) log in the control group,showing a significant difference between them (t =3.251,P＜0.01).The respective mean C-quant measurements for hard nucleus cataract,cortical cataract,nuclear-cortical cataract and posterior subcapsular cataract patients were (1.96±0.42) log,(1.91 ±0.16) log,(2.05 ±0.19) log and (2.48 ±0.66) log,respectively,with a significant difference among these four groups (F =2.156,P =0.019).The highest straylight value was detected in the posterior subcapsular cataract group.The straylight value was enlarged with the increase of age with a regression equation of Y=0.0010X+ 1.025 in the hard nucleus cataract group (r =0.455,P ＜ 0.05).In addition,the negative linear correlation was found between the straylight value and BCVA in both the hard nucleus cataract group and cortical cataract group (r=-0.590,-0.697,P＜0.01).However,no correlation was found in the mixed cataract group and posterior subcapsular cataract group (r =-0.240,-0.235,P＞0.05).Conclusions The C-quant straylight meter can objectively reflect the visual function for hard nucleus and cortical cataractous eye.Posterior subcapsular cataract produces straylight and exerts a great influence on visual quality due to early glare sensation,so it should be benefit to perform surgery earlier.

Objective To study the cognitive behaviors of ICU nurses about cluster intervention strategies of central venous catheter blood infection.Methods Two hundred and two ICU nurses were involved in the survey using self-designed questionnaire.Results The scores on knowledge of ICU nurses with cluster intervention strategies was(31.9±2.8)and the score on the cognitive behaviors was(26.9±2.6). Conclusions The ICU nurses’knowledge on cluster intervention strategies is at a general level and their behaviors are at the status ofseldomorsometimes.Therefore,improvement of manipulation flowsheet,control of routes for bacterial infections and enhancement of knowledge learning are critical for the improvement of cognitive behaviors of ICU nurses with cluster intervention strategies.

The paper collects patents relevant to hypolipidemic drugs in recent decade based on the Derwent database,reveals the development process of drug research in this field by taking advantage of the patent analysis method,and discusses the change of application quantity for patents over the years,distribution in the hotspot technology field and the main application organizations and their R&D orientations.

Objective To explore the clinicopathological and immunohistochemical features of wolffian adnexal tumour ( WAT) . Meth-ods The clinical and pathological features analysis and immunohistochemistry methods were utilized to study the histopathology features of a case Wolffian adnexal tumor. Results One case of unilateral WAT which located in broad ligament display a solid-cystic mass with pedicled and enveloped,its histology showed large and small tubulars,screen structure and solid zone. Its immunohistochemistry staining was positive for pan-cytokeratin,vimentin,CK18 and CD99,positive staining for α-inhibin in a little tumor cell,and negative for calretinin,CD10,EMA, ER,PR,Syn,CA15-3,CA19-9,Ki-67 index is less than 5%. PAS positive staining for the basement membrane around Gland like structure. Conclusion Wolffian adnexal tumour is diagnosised by its histopathological,immunohistochemical and it’ s distinctive location where Wolffi-an duct remnants are found.

To find out the patterns and trend of clinical use of antihypertensive agents to provide a guidance for rational use of drugs, the antihypertensive drugs used in our hospital during 1995-1999 period was investigated in respect to the sum of consumption cost, drugs used and DDDs. Our results showed that the DDDs and the consumption cost for antihypertensive drugs have increased gradually for recent 5 years. The order of DDDs of drugs was as follows: calcium antagonists, adrenergic blocking drugs, ACE inhibitors, the drugs affecting sympathetic transmitters and peripheral vasodilators. The order of sum of money was as follows: ACE inhibitors, calcium antagonists and calcium antagonists respectively. It is concluded that a diverse array of anthhypertensive agents were available and widely used clinically and attention should be paid to the rational use of the drugs.

Objective To study the effect of erythropoietin on cardiac function of the patients with acute mycardial infarction (AMI). Methods 48 patients with AMI successfully treated with thrombolytic therapy were randomized into two group,2000 units of recombinant human erytfu-opoietin(rh-EPO) were administrated once a time in therapeutic group,3 times on alternate days in one week and total for 4 weeks. The peak value of serum creatine kinase(CK) and creatinkinase isozyme MB (CK-MB)were measured, myocardial infarct size (S) was estimated by Hindmen's QRS scoring system, and the diameter of left ventricular end diastolic(LVEDd) and left ventricular injection fraction (LVEF) were determined with echocardiography at the 4th weekend in both groups. Results CK, CKMB and S in therapeutic group were lower than in control group (P < 0.05). LVEDd and LVEF were also improved in the therapeutic group. Conclusion rh-EPO can significantly lessen the size of isehemia and infarct myocardium, mitigate the infarction degree and improve the cardiac function slightly in AMI patients.