BACKGROUND: Vitapex is an injectable root canal filling material. It possesses effects of antibiosis, analgesia, as well as promotes formation of alveolar bone, dentin and cementum, which had been widely used in apexification. However, it also has weakness of long induction time and need reduplicated subsequent visit.OBJECTIVE: To explore the therapeutic effect of mineral trioxide aggregate (MTA) and Vitapex paste on apexification.DESIGN, TIME AND SETTING: The contrast observation experiment was performed at the Department of Stomatology,Shenyang Children's Hospital from February 2006 to November 2008.PARTICIPANTS: A total of 42 patients with 49 teeth were included in this study, including 28 anterior maxilla and 21 premolars.The X-ray film showed that the root tip of teeth was immature.METHODS: The teeth were randomly divided into the experimental group (n=25) and control group (n=24), which was apexificated by MTA and Vitapex paste, respectively.MAIN OUTCOME MEASURES: The effective rate of two groups was compared at months 3, 6 and 12 after operation.RESULTS: All teeth were included in the final analysis. The effective rate of experimental group was greater than the control group at 3 months after operation, and the difference had significant (P < 0.05), while, the effective rate was similar in two groups at 6 and 12 months after operation (P > 0.05).CONCLUSION: Both MTA and Vitapex paste are ideal filling materials in apexfication. Nevertheless, MTA may be a substitute in apexfication for its short therapy duration and precisely therapeutic effect.

Objective To study the detection and significance of human papillomaviral DNA(HPV-DNA) in cervical disease. Methods The express of HPV-DNA were detected by hybrid capture 2 in the patients with chronic cervicitis ( n = 166), cervical intraepithelial neolasia ( GIN ) ( n = 89 ) and uterine cervix cancer ( UCC ) ( n = 23 ). Results The positive expression rate of HPV-DNA in GIN Ⅰ , Ⅱ , Ⅲ grade and UCC were 70.8% (17/24) ,79. 3% (23/29) ,88.9% (32/36) ,91. 3% (21/23). They were significantly higher than those in chronic cervicitis 30.7% (51/166) (P ＜ 0. 01 );As the development of cervical disease, HPV-DNA positive expression had increasing trend.Conclusion The detection of HPV-DNA had an important role for the early UCC screening,prevention and treatment.

Comprehensive treatment is the hot for cervical carcinoma,but comprehensive treatment is not the superposition of several treatment methods.Surgical treatment is the main means for early cervical carcinoma and radiotherapy for locally advanced. It's sure that radiotherapy combined with hyperthermia.Abroad,concurrent chemotherapy and radiotherapy is regarded as routine treatment methods.Improvement of hyperthermia technology is more applicated in the clinical.Reducing complications after treatment and enhancing the effectiveness of the treatment of cervical carcinoma is the ultimate goal.

Objective To evaluate the asynchrony of right ventricle(RV) and its significance in idiopathic pulmonary artery hypertension(IPAH) patients by quantitative tissue velocity imaging.Methods Twenty-five normal people,26 IPAH patients were studied.RV structure parameter(thickness of lateral wall,late diastolic and systolic area and fractional area change) and function parameter [peak systolic velocity(Sa),early diastolic velocity(Ea),late diastolic velocity of tricuspid annulus of lateral wall and Tei index] were measured and calculated.Regional velocity profiles of 6 segments of RV were obtained.The time from onset of QRS complexes to Sa and Ea(T_(Q-S),T_(Q-E)) of 6 segments were measured.The maximal difference in T_(Q-S) and T_(Q-E) in the same wall(Intra-?T_(Q-S),Intra-?T_(Q-E)),the same segment(Inter-?T_(Q-S),Inter-?T_(Q-E)) and all 6 segments(Max-?T_(Q-S),Max-?T_(Q-E)) were calculated.Results Compared with controls,Inter-?T_(Q-S),Inter-?T_(Q-E),Max-?T_(Q-S) and Max-?T_(Q-E) were significantly prolonged in IPAH patients(P(0.05)).Max-?T_(Q-S) and Max-?T_(Q-E) were correlated with structure and function parameter in IPAH patients.Conclusions Asynchrony exists in RV and have good relation with RV structure and function in IPAH patients.

Dysregulation and activation of immune processes are important in age-related macular degeneration (AMD) pathogenesis.The single nucleotide polymorphism of complement factor H is widely recognized as a risk factor to AMD.Over-activation of nod-like receptor3 and polymorphism of Toll-Like Receptor 3 also associated with AMD.Except for innate immune processes,adaptive immunity also play a critical role in AMD,a growing body of evidence supports that auto-antibodies and T cells are related with AMD.Additionally A2E and lipid oxidation byproducts might also have a role in AMD pathogenesis.

[Summary] Chronic suppurative otitis media affects patients ’ quality of life, and is life-threatening in patients with cholesteatoma which causes intracranial or extracranial complications .The purpose of surgery is to clear up all the lesions and to reconstruct hearing function .Tympanoplasty is the main surgical method , which includes basically canal wall down ( CWD) and canal wall up (CWU) styles.CWD provides lager surgical field but impairs normal auditory canal structure ; CWU preserves the posterior wall of external auditory canal and provides better quality of life although it has higher possibility of recurrence of cholesteatoma .We reviewed the surgical methods , surgical effects , recurrence rates , and surgical indications of canal wall up mastoid-tympanoplasty , hoping to provide meaningful reference for clinical application .

From 1952 to 1990, 21 cases of malignant tumor of the appendix, including 14 cases of carcinoid tumor of appendix, 4 cases of appendix adenocarcinoma, 2 cases of appendix lymposarcoma and 1 case of appendix cystocarcinoma, were treated in the Affiliated Hospital of Wannan Medical College Among these 21 patients, 16 underwent appendectomy alone, 5 underwent appendectomy first and a right hemicolectomy was performed on later All of the 21 patients were followed up for 5～10 years,the survival rate was 90 5% and 90 5% respectively So if a malignant tumor of the appendix was suspected, a biopsy during operation should be done to determine the pathological type If the diagnosis was clear, the choice of operation mode must be made according to the size and the pathological type of the tumor In selective patient, a right hemicolectomy must be performed on

Objectives To study the frequency and risk factors of osteoporosis,and the relationship between bone turnover makers and bone mineral density (BMD) in patients with ankylosing spondylitis (AS).Methods BMD in the spine and femoral neck was measured with dual-energy x-ray absorptiometry (DEXA) in 30 AS patients. Enzyme linked immunoassay was used to measure serum bone gla protein (BGP), bone-specific alkalin phosphtase (BAP), and urine deoxypyridinoline crosslinks (D Pyr).Results The patients in early stage lost the bone mass already.They had lower volume of BMD in both hip and spines than that of controls. But in the later stage,the BMD of spines was higher than that of the control group because of the calcification of the surrounding tissues of the spine.While even the BMD of femoral neck was still lower than that of normal subjects. Osteoporosis was observed in 6 patients, osteopenia in 10. Osteoporosis in AS correlated negatively with the disease duration, ESR, CRP, and X ray grades. The reduction in BMD in premenopausal women with early AS was slighter than that in men. The BMD had no significant differences in HLA B 27 (+) patients and HLA B 27 (-)patients. BGP and BAP which reflect bone formation had no differences in AS patients and controls. D Pyr which reflects bone resorption was significantly increased compared with controls.Conclusions Osteoporosis in AS is common and may be related to disease duration and severity. A significant increase in bone resorption is observed in AS with osteoporosis.

Objective To explore the clinical diagnostic value of CYFRA21-1 in benign lung disease deriving from epithelium cells.Methods CYFRA21-1 level in serum and hydrothorax was determined by electrochemiluminescence immunoassay. The complex sandwich (KS 19.1 monoclonal antibody-CYFRA21-1[Ru(bpy)3]2+ labeled monoclonal antibody BMI 9.21) was fixed to the bead enclosed avidin by means of biotin-avidin combination, then the luminescence under the influence of perfluorotripropylamine and electric field luminous was tested for CYFRA21-1.Results The level of CYFRA21-1 in the group suffering from lung disease accompanied by complications was significantly higher than that of single lung disease group (P<0.01). CYFRA21-1 value in the elder group was significantly higher than that of the younger group (P<0.01).Conclusion The gerontal patients suffering from benign lung disease accompanied by complications should be given symptomatic treatment if their CYFRA21-1 level is elevated but CEA and NES was normal. Recheck the above indices two weeks later so as to identify the benign or malignancy of the disease.

Objective To prepare paclitaxel-loaded poly(ε-caprolactone)(PCL)/pluronic F68(F68)blend microspheres as a controlled release system. Methods Paclitaxel-loaded PCL/F68 blend microspheres were prepared by the oil-in water(O/W)emulsion/solvent evaporation method. Characterization of the microspheres followed to examine the particle size, the drug encapsulation efficiency, the surface morphology, in vitro release behavior and DSC analysis. In vivo antitumor activity of paclitaxel-loaded PCL/F68 blend microspheres was evaluated in mice bearing with hepatoma H22 cells ascites tumor. Results The results showed that the porous structure can be formed in the surface of PCL/F68 blend microspheres. Faster and controlled release of paclitaxel from PCL/F68 blend microspheres was achieved in comparison with the PCL microspheres. In animal tests, paclitaxel-loaded PCL/F68 blend microspheres showed the potent antitumor activity against hepatoma H22 cells in ascites tumor model. Conclusion The paclitaxel loaded PCL/F68 blend microspheres were found to own a faster release rate and a remarkably controlled release behavior.