This study was performed in order to evaluate the sero-epidemiological status of toxoplasmosis in pregnant Korean women. Among 5, 175 sera and 750 amniotic fluid samples obtained from pregnant women, 41 serum samples (0.79%) and 10 (1.33%) amniotic fluid samples tested positive for IgG antibodies by ELISA. Fifty one cases showing a score more than 0.25 on ELISA were tested for PCR reaction against the SAG1 gene. Only one case of the 51 ELISA positive cases exhibited a positive reaction on all tests. This case had a history of acute nephropyelitis during early pregnancy, but fortunately, had delivered a phenotypically healthy baby. In this study, the seroprevalence of toxoplasmosis in pregnant women was found to be comparatively low, consistent with previous reports from Korea. However our trials, performed with a variety of diagnostic tools, were considered to be useful for the precise diagnosis of congenital toxoplasmosis.
Adult ; Animals ; Antibodies, Protozoan/*blood ; Female ; Humans ; Korea/epidemiology ; Pregnancy ; Pregnancy Complications/diet therapy/*epidemiology ; Seroepidemiologic Studies ; Toxoplasma/*immunology ; Toxoplasmosis/*epidemiology

PURPOSE: This study aimed at not only suggest some ideas useful in planning and doing the safety education in the elementary schools in the near future putting into test the safety education proposal which has been developed by researchers in accordance with the national 7st education courses but also improve health of the children through taking right safety knowledge and sound safety attitude. METHOD: For this study 140 student are selected from 3-5th grade of the elementary school in Kyoungki-Do and Kwangwon-Do and have been taught the safety education during 10weeks, 10times. RESULT: 1) After the safety education, knowledge about the injury prevention of the children show to be significantly different from that they had before(t=-9.156, p=.000). 2) In order to look into any effect of the attitude about injury prevention after the safety education there were significant difference(t=-3.755, p=.000).CONCLUSION: As the findings of the study indicating that safety education expand the correct knowledge about injury prevention and have more affirmative and sound attitude about injury prevention. So it may be concluded that, safety education have the positive effect both knowledge and attitude about injury prevention among elementary school children.
Child* ; Education* ; Gangwon-do ; Humans ; Child Health

No abstract available.

Objective: We investigated the factors that affect the time from the onset of acute stroke symptoms to arrival at the emergency department (ED). Methods: Between July 2019 and February 2020, patients with acute stroke symptoms who visited a public hospital were evaluated by retrospective analysis using a prospective survey. The data for this study was gathered from baseline interviews with patients enrolled in stroke surveys. The primary outcome was time to arrival at the ED, evaluated as a binary variable with a value of 4.5 hours. Results: Overall, 205 patients were included in the final analysis. Among them, 47% (n=96) of patients with acute stroke symptoms arrived at the ED later than 4.5 hours from the time of onset of acute stroke symptoms. After multivariable logistic regression analyses, it was found that patients with higher National Institutes of Health Stroke Scale (NIHSS) scores, patients who did not live with their family members, and patients who were found by a passerby were associated with early ED arrival post onset of acute stroke symptoms. Among patients diagnosed with acute stroke, those with higher NIHSS scores or underlying malignant diseases were found to present earlier at the ED. Conclusion Approximately half of the patients (47%) with acute stroke symptoms presented to the ED within 4.5 hours. Higher NIHSS scores and the presence of underlying malignancy were identified as the key factors that were associated with an early presentation at the ED from the time of onset of acute stroke.

PURPOSE: To report a case of Chandler's syndrome associated with anterior uveitis. METHODS: We encountered a 39-year-old woman who complained of decreased visual acuity in the left eye with anterior uveitis and increased intraocular pressure. On examination, we found corectopia, endothelial pleomorphism and peripheral anterior synechiae, leading to the diagnosis of Chandler's syndrome with anterior uveitis. RESULTS: The anterior uveitis and increased intraocular pressure in Chandler's syndrome were well controlled with the use of Beta-blocker, Latanoprost and topical corticosteroids. We report this case with a review of the literatures.
Adrenal Cortex Hormones ; Adult ; Diagnosis ; Female ; Humans ; Intraocular Pressure ; Iridocorneal Endothelial Syndrome* ; Uveitis, Anterior* ; Visual Acuity

PURPOSE: To report a case of Chandler's syndrome associated with anterior uveitis. METHODS: We encountered a 39-year-old woman who complained of decreased visual acuity in the left eye with anterior uveitis and increased intraocular pressure. On examination, we found corectopia, endothelial pleomorphism and peripheral anterior synechiae, leading to the diagnosis of Chandler's syndrome with anterior uveitis. RESULTS: The anterior uveitis and increased intraocular pressure in Chandler's syndrome were well controlled with the use of Beta-blocker, Latanoprost and topical corticosteroids. We report this case with a review of the literatures.
Adrenal Cortex Hormones ; Adult ; Diagnosis ; Female ; Humans ; Intraocular Pressure ; Iridocorneal Endothelial Syndrome* ; Uveitis, Anterior* ; Visual Acuity

No abstract available.
Aged ; Biopsy ; Humans ; Liver Cirrhosis/diagnosis/*etiology/therapy ; Male ; Pericarditis, Constrictive/*complications/diagnosis/therapy ; Tomography, X-Ray Computed

The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed the pseudopodia-specific localization by immunocytochemistry in the previous study. Based on the idea that the pseudopodia-specific Nfa1 protein mentioned above seems to be involved in the pathogenicity of N. fowleri, we observed the effect of an anti-Nfa1 antibody on the proliferation of N. fowleri trophozoites and the cytotoxicity of N. fowleri trophozoites on the target cells. The proliferation of N. fowleri trophozoites was inhibited after being treated with an anti-Nfa1 polyclonal antibody in a dose-dependent manner for 48 hrs. By a light microscope, CHO cells co-cultured with N. fowleri trophozoites (group I) for 48 hrs showed severe morphological destruction. On the contrary, CHO cells co-cultured with N. fowleri trophozoites and anti-Nfa1 polyclonal antibody (1: 100 dilution) (group II) showed less destruction. In the LDH release assay results, group I showed 50.6% cytotoxicity, and group II showed 39.3%. Consequently, addition of an anti-Nfa1 polyclonal antibody produced a decreasing effect of in vitro cytotoxicity of N. fowleri in a dosedependent manner.
Animals ; Antibodies, Protozoan/*immunology ; Antigens, Protozoan/genetics/*immunology ; CHO Cells ; Dose-Response Relationship, Immunologic ; Female ; Hamsters ; Mice ; Mice, Inbred BALB C ; Naegleria fowleri/growth & development/immunology/*pathogenicity ; Protozoan Proteins/genetics/*immunology ; Recombinant Proteins/immunology ; Support, Non-U.S. Gov't

In situ hybridization using biotinylated HPV(Human papillomaviruses) probes was performed to detect HPV DNA in 24 patients with genital wart-like lesions. The lesions were divided into two groups, with or without dysplastic changes histologically. We could detect HPV6/11 in 13 of 17 lesions(76.5%) without dysplastic changes. HPU16/18 was detected in a case with dysplastic changes. HPV6/ll was also detected in a case considered to be misdiagnosed as bowenoid papulosis. Oncogenic HPV such as HPV16/18 was found in one of histologically splastic lesions(14.3%).
Condylomata Acuminata* ; DNA* ; Humans* ; In Situ Hybridization

PURPOSE: No valuable early marker exists for the prognosis of patients resuscitated from out-of-hospital cardiac arrest (OHCA). We compared post-resuscitation levels of several biochemical candidates and correlated them with outcomes of cardiopulmonary resuscitation (CPR). METHODS: Eligible resuscitated patients with OHCA were prospectively enrolled from December 1, 2004 to November 30, 2005. Demographic data were collected and early levels of biochemical candidates were measured both at the time of initiation of CPR and immediately after the return of spontaneous circulation (ROSC), including those of ischemia-modified albumin (IMA), procalcitonin, and S-100 protein. The ROSC over 20 minutes, survival from the emergency room (ER), survival after 1 month, and Glasgow Outcome Scale (GOS) after 1 month were evaluated for outcome. Levels of early biochemical candidates were correlated with those outcomes. RESULTS: 126 patients were eligible for analysis. 81 (64.3%) were male, and the mean age was 59.6+/-17.2 years. The rate of ROSC, survival from ER, and survival after 1 month were 40.5%, 33.3%, and 8.7%, respectively. 4 patients whose GOS was more than 4 showed good neurological outcome. The levels of S-100 protein were significantly lower in the survival group than in the non-survival group after 1 month: 1.6+/-0.8 versus 3.9+/-4.9 microgram/L at the time of initiation of CPR (p<0.01), and 1.6+/-1.1 versus 4.5+/-5.0 microgram/L immediately after ROSC (p=0.01), respectively. IMA and procalcitonin did not show any predictive value. CONCLUSION : The levels of S-100 protein was found to be useful as an early biochemical marker for prognosis following OHCA. S-100 concentrations were significantly lower in the survival group than in the non-survival group after 1 month after successful resuscitation from OHCA.
Biomarkers* ; Cardiopulmonary Resuscitation ; Emergency Service, Hospital ; Glasgow Outcome Scale ; Heart Arrest ; Humans ; Ischemia* ; Male ; Out-of-Hospital Cardiac Arrest* ; Prognosis* ; Prospective Studies ; Resuscitation ; S100 Proteins*