This study was performed to investigate the heavy metal contents of freshwater fishes. The samples of 24 species were collected at 7 areas located on the Mankyung River during September in 1987. And then the contents of lead, cadmium, copper and zine were analyzed by atomic absorption spectrophotometer. The results were summarized as follows ; 1. The mean value of lead, cadmium, and copper contents of fishes collected in the downstream were significantly higher than those of upstream. 2. The mean lead content of C. auratus was the highest 1.50+/-0.98 microgram/g in viscera and statistically significant difference from muscle content. 3. The mean cadmium content of C. auratus was the highest 0.087+/-0.054 microgram/g and significantly higher than that of muscle, skeleton and gill. 4. In the copper contents, the viscera of C. auratus was the highest 5.25+/-0.94 microgram/g and significantly higher than that of muscle, skeleton and gill. 5. The mean value of zinc content of C. auratus was shown the order of gill, skeleton, viscera and muscle.
Absorption ; Animals ; Cadmium ; Copper ; Fishes* ; Fresh Water* ; Gills ; Rivers* ; Skeleton ; Viscera ; Zinc

PURPOSE:Obesity is associated with complications such as unfavorable lipid profiles, hyperinsulinemia, and hypertension. We investigated the degree of obesity, especially defined as BMI and the percentage of overweight for the standard weight (%Wt) on plasma lipid levels and insulin resistance in the pediatric age group. METHODS:In this cross sectional study, 64 children and adolescents were included. Anthropometric data including body weight, height, BMI, %Wt, fat percentage of body weight (Fat%), and waist to hip ratio (WHR) were collected. For metabolic data, 14 hour fasting serum glucose, insulin and lipid profiles were measured and index for insulin resistance (HOMA-IR, FGIR) were calculated. We analyzed if there exist relationship between each anthropometric data and metabolic data. Gender differences were also assessed. RESULTS:Hyperinsulinemia showed high prevalence in obese children (total=21.9%, male=22.5%, female= 20.8%). HOMA-IR correlated with BMI and %Wt (BMI:r=0.308, P<0.05; %Wt:r=0.414, P<0.05). FGIR also correlated with BMI and %Wt (BMI:r=-0.406, P<0.05; %Wt:r=-0.437, P<0.05). Fasting serum triglyceride levels showed statistically significant correlation with BMI and %Wt [Boys (BMI:r=0.334, P<0.05; %Wt: r=0.487, P<0.05), Girls (BMI:r=0.482, P<0.05; %Wt:0.406, P<0.05)]. CONCLUSION: BMI and %Wt were valuable for predicting insulin resistance and triglyceride level. Sexual dimorphism related to insulin resistance and dyslipidemia in obese children was not certain in this study.
Adolescent ; Blood Glucose ; Body Weight ; Child* ; Dyslipidemias ; Fasting ; Female ; Humans ; Hyperinsulinism ; Hypertension ; Insulin Resistance* ; Insulin* ; Obesity ; Overweight ; Plasma ; Prevalence ; Triglycerides ; Waist-Hip Ratio

We reviewed twelve cases of congenital dislocation of the knee(CDK) which treated at Keimyung University from January 1992 to January 1997. All case were type II dislocation according to classification suggested by Ferris and reduced with minimal treatment. Dislocation were reduced within 4 days in minimum after treatment and 3 weeks in maximum. The period of follow up ranged from 6 months to 5 years. Clinical results were good. Early diagnosis followed by conservative treatment is important and enough to treatment of the type II congenital dislocation of the knee.
Classification ; Dislocations* ; Early Diagnosis ; Follow-Up Studies ; Knee*

BACKGROUND: Numerous studies demonstrated that genistein induced the decrease of proliferation and apoptosis in a variety of cells. However, there is no report about the effect of genistein on proliferation and demise of mast cells. OBJECTIVE: This study was conducted to investigate genistein-induced aoptosis of mast cells as it pertains to both its basic drug mechanism and the potential therapeutics of the pathologic conditions accompanying mast cell proliferation. METHODS: p815 murine mastcytoma cell line was used to assess the effects of genistein treatment including viability and proliferation, morphlolgic study, DNA electrophoresis, the effect of caspase inhibitor, western blotting, and mitochondrial event. RESULTS: Genistein indeced many apoptotic manifestations as evidenced by changes in cell morphology, generation of DNA fragmentation, activation of caspase 3, and DNA hypoploidy. The reduction of mitochondrial membrae potential and the release of cytochrome c to cytosol were also demonstrated. However, reduction of mitochondrial membrane potential and cytochrome c release were not prevented by caspase inhibitors zVAD-fmk and BocD.fmk, or PTP(permeability transition pore) blockers such as bongkrekic acid and cyclosporin A. CONCLUSIONS: This in vitro study suggests that pathologic increases in mast cell number possibly be regulated in vivo by therapeutic strategy enhancing apoptosis by treatment of genistein.
Apoptosis* ; Blotting, Western ; Bongkrekic Acid ; Caspase 3 ; Caspase Inhibitors ; Cell Line ; Cyclosporine ; Cytochromes c ; Cytosol ; DNA ; DNA Fragmentation ; Electrophoresis ; Genistein ; Mast Cells ; Mastocytoma* ; Membrane Potential, Mitochondrial

No abstract available.

The positive binding activity of lectin, peanut agglutinin (PNA), against the mucinous malignancies of the ovary was studied in order to clarify biologic differences among those lesions using immunoperoxidase method (ABC). A total of 23 cases were included in this study and they were classified as 10 cases of mucinous cystadenocarcinoma, 9 mucinous tumors of borderline malignancy and 4 pseudomyxoma peritonei, histologically. Nine of 10 cystadenocarcinomas and all cases of pseuomyxoma peritonei showed more than moderate degree of positive binding activity (>2+) with PNA in the neoplastic epithelial cells. In the cases of borderline malignancy, only 3 of 9 revealed as much similar binding pattern with PNA as cystadenocarcinoma group, in contrast, minimal degree of positivity (1+) was noted in the remainder. These findings may suggest heterogeneity in the biochemical characteristics among the cases of borderline lesion. And it is proposed that the higher PNA binding cases in ovarian mucinous borderline malignancy require extensive sampling by multiple sections and further careful follow-up study.
Follow-Up Studies

Authors studied on the Acaroid mite in the food, and the following results were obtained. 1) 6 species of Acaroid mite were found among twelve kinds different types of food. a. Tyrophagus dimidiatus, b. Glycyphagus destructor, c. Glycyphagus domesticus, d. Hoshikadania konoi, e. Acarus siro, f. Carphoglyphus lactis. 2) Acaroid mite were not found in the wheat flour and magalin among the twelve different kinds. 3) Acaroid mit were distributed at higher rate in the pollack(94%), cuttle fish(77%) and anchovy(50%).
Epidemiology* ; Flour ; Mites* ; Triticum

Liver cell dysplasia of Anthony(LCD) is a common association in hepatocellular carcinoma(HCC)-bearing liver and has been regarded as a premalignant condition with strong linkage to hepatitis B virus infection and cirrhosis. A total of 189 surgically resected livers [HCC(168 cases), cholangiocarcinoma(3 cases), metastatic carcinoma(3 cases), and non-neoplastic lesions(15 cases)] were reviewed to elucidate the nature of LCD by means of light microscopic examination, in situ hybridization method for HBV DNA and expression of proliferatin cell nuclear antigen(PCNA) using immunohistochemical technique. LCD was present in 141 cases(74.6%), and its prevalence rate was independently significant in HCCs with or without cirrhosis than other groups. There was no difference in mean age, although LCD-positive group was younger than its negative counterpart. Association rate of LCD in HCC-cirrhosis group was statistically significant than the non-cirrhotic group, and higher histological grading of LCD was correlated well with wider distribution pattern and clustering. Seropositivity of HBsAg was not correlated with presence of LCD or with histological grading. In situ hybridization techique using HBV DNA probe demonstrated fine granular stainable particles even in LCD cells. Immunohistochemical study for PCNA revealed that the proliferative activity of LCD was lower than that of the cirrhotic cell. With the above results it is concluded that LCD reflects neither a regenerating condition nor a premalignant lesion but suggest a reactive change.
Carcinoma, Hepatocellular ; Neoplasm Metastasis

No abstract available.
Thyroid Gland* ; Thyroid Nodule*

No abstract available.
Keratosis, Seborrheic ; Skin Diseases ; Skin Diseases, Papulosquamous