Systemic contact dermatitis (SCD) is a kind of allergic inflammatory skin disease caused by contact with the same or cross-reactive allergens via systemic absorption in individuals who have been exposed to allergens. SCD lesions are diverse and easily overlooked in clinical practice. This review summarizes progress in clinical manifestations, pathogenesis, diagnosis, treatment of and sensitizers in SCD, aiming to improve its clinical diagnosis and treatment.

0.05), but insulin sensitivity was actually improved (P

To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
Animals ; Apoptosis ; Breast Neoplasms ; drug therapy ; pathology ; Humans ; Inhibitor of Apoptosis Proteins ; genetics ; pharmacology ; Injections, Intralesional ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Transfection

Animals ; Carcinogenicity Tests ; Cytotoxicity Tests, Immunologic ; Humans ; Mutagenicity Tests ; Soot ; toxicity

Objective To clone the full length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells (DPC). Analyze its characteristics and predict its biological function in the phase of growth and differentiation for DPC. Methods Rapid amplification of cDNA ends (RACE) technology was used for full length cDNA amplification. Bioinformtic methods were used to analyze the chromosome location, protein sequence, domain and possible biological function of the gene. Results Two isoforms of HSPC016 gene were obtained from DPC. They were 400bp and 493bp, respectively. The gene was mapped on chromosome 3 q21 31, and was conservative on evolution. HSPC016 protein had 64aa, belonged to PD053992 protein family; its functional domain was homologous to T2FA gene. Conclusion HSPC016 was a transcriptional modulatory gene. Its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through modulating other genes' transcription within nucleus

Objective To investigate the role of transforming growth factor-?_1 (TGF-?_1)and hepatocyte growth factor(HGF)in the pathogenesis of early renal impairment in patients with chronic hepatitis B(CHB).Methods The serum levels of TGF-?_1 and HGF of 44 patients with CHB and 20 healthy people were measured by specific-ELISA,and the urine levels of ?_1-microglobulin (?_1-MG)and microalbumin (mALB)were tested by RIA.Results The serum levels of TGF-?_1 and HGF in the patients with CHB were significantly higher than those in the control (P

Objective: To discuss the university students early-stage interpersonal perception and selection in internet Method; Totally 362 university students who used internet instant chat system were surveyed with the self-designed questionnaires. They were required to assess the influence degree of internet information on their feeling and choosing in a five degree scale. The differences in gender, subject and lasting time were also compared. Re-sults: (1) Age (4. 39 ± 1.94), nick name (3.71 ± 1.92) and self-explained (3.67 ± 1.97) were the most important information for choosing a friend in internet, which were higher than three most unimportant information including blood type (1.23 ±0. 80), level (1.40 ±0.95), and operation (1.41 ±0.89) (P<0.01) . (2) All students trended to evaluate others or selves information veritable. In all items, the veracity rate of self evaluate was high than 78.2% , and the veracity degrees of evaluating others'information were between 2.05 ～ 2.91. (3) Bad manners (1. 33 ±0.72), rough speech (1.41 ±0.71), and bored dialogue (1.50 ±0. 80) were the most related factors to choose friend, which were higher than three most unimportant information including no meeting chance (4.47±0.94), slow typing (3.13 ±1.12), poor motivation (2.32 ± 1.05) (P<0.01) . Conclusion: This study suggests that peoples early-stage perception and selection in internet are based on the selection, confirmation and conjecture of users, which will have certain effects on the development of network interpersonal relationship.

BACKGROUND:An increase in activity of muscle contraction can induce transcription of a variety of signaling molecules to activate a large number of gene expression within the nucleus through proprietary signaling pathway OBJECTIVE:To review studies related the peroxisome proliferator-activated receptor-gamma coactivator 1(PGC-1)and exercise induced skeletal muscle adaptations.METHODS:A computer-based online search of Pubmed was performed for relevant English articles published from January 1995 to January 2009 with the keywords of"PGC1,skeletal muscle,exercise,adaptations"The relevant articles about PGC-1 and exercise induced skeletaI muscle adaptations were included.and repetitive contents were excluded.PGC1 and mitochondrial oxidative metabolism,and exercise-induced skeletal muscle adaptation and PGC1s changed served as the evaluation indicators.RESULTS AND CONCLUSION:Of 57 collected articles,34 were classified and sorted according to the criteria.Endurance training results in profound adaptations for skeletal muscle,including mitochondrial biogenesis,capillary density,and fiber composition.Transcription factors are highly dependent on coactivator molecules to regulate training physiologic adaptation processes.The majority of transcription factors target genes are involved in mitochondrial biogenesis and metabolism.These transcriptional patterns may provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that exercise induced energetic properties Post-transcription of PGC-1 alpha protein,there are a variety of protein modifications,with a variety of biological processes closely related to the possible adaptive mechanism of exercise-induced skeletal muscle.

Objective To establish the optimal section planes for observing ventricular septal defect(VSD) by real-time three dimensional echocardiography(RT-3DE).Methods In 6 pig hearts with different types of VSD, after high pressural perfusion with coupling reagent, two dimensional, dual plane and RT-3DE were performed with different section planes, estimating with half quantificational visional grade:4 marks,very clear;3 marks,clear;2 marks,not clear enough; 1 marks,not clear. Results Observation by RT-3DE, en-face view of atrial-ventricular(S2a) and en-face view of left ventricular septum(L1a) provided relatively ideal section planes in sub-arterial ventricular septal defect, S2a and en-face view of right ventricular septum(L2a) provided relatively ideal section planes in perimembranous and muscular VSD. Conclusions S2a reflects relatively ideal section plane in sub-arterial, perimembranous and muscular VSD.

BACKGROUND: The augmenter of liver regeneration (ALR) is a kind of important secondary liver regeneration factors, and it can particularity promote the liver regeneration. OBJECTIVE: To clone the RNA extracted from neonatal rat liver to ALR gene, and to analyze the gene using the bioinformatics. DESIGN, TIME AND SETTING: The study, gene molecule in vitro experiment, was performed at the Laboratory of Neurobiology, College of Life Science of Henan University between March 2008 and May 2008. MATERIALS: The liver tissues were harvested from 3-day-old Wistar rat of SPF grade. METHODS: Total RNA was exacted from rat liver tissues using guanidinium isothiocyanate-phenol-chloroform extraction method. According to the guideline of molecular biology experiment, the target fragment was amplified using reverse transcription-polymerase chain reaction. Alter purified, the target gene was recovered with gel recovery kit and subcloned to pGEM-T easy, and then was transformed to E.coli. The extracted piasmid was sequenced by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd. The sequence was analyzed with molecular biological software. MAIN OUTCOME MEASURES: Polymerase chain reaction amplification band; analysis of gene sequence; sedne, threonine and tyrosine phosphorylation predictions; phytogenetic analysis of ALR. RESULTS: The potymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio redo. CONCLUSION: In this experiment, we have cloned the ALR gene successfully.