0.05).The prevalence rates of ID in first trimester group and second trimester group were 14.29% and 33.81%,respectively,showing significantly higher rate in the latter group(?2=6.33,P

Objective:To better understand the karyotype distribution ofthe patients with Down's syndrome(DS)who have been finally diagnosed in our hospital.To compare the strengths of G-banding karyotype analysis and FISH(Fluorescence In Situ Hybridization, FISH)in diagnosing the DS patients with the Chromosome mosaicism and Calculating the percentage of abnormal karyotypes.Methods:Blood of the patients who were likely to suffer from DS was selected for G-banding karyotype analysis to describe the karyotype distribution.5 of the finally diagnosed patients with the chromosome mosaicism were randomly selected for FISH to compare the percentage of abnormal karyotypes of the two methods.Results:287 DS patients were diagnosed,91.29%of whom had the simple karyotype.Among the patients with the translocation karyotype, 11 D/G translocation karyotype were detected,accounting for 73.33%,while 4 G/G translocation karyotype were diagnosed, constituting 26.67%.One patient with both of the translocation karyotype and the mosaicism karyotype was detected in our research,which was uncommon in recent report.The results of FISH and G-banding karyotype analysis were consistent.There was no significant difference of the abnormal karyotype rates for DS patients with the chromosome mosaicism by G-banding karyotype analysis and FISH.Conclusion:The simple karyotype is the main karyotype of the DS patients in our research.Among the translocation karyotype,the D/G kind is common.DS patients can be diagnosed efficiently by G-banding karyotype analysis,but we still suggest to use FISH to get the proportion of the abnormal cell for the patients who have less triploid cell and slighter clinical manifestation.

With the development of antibody manufacturing technology and improvement of new drug research in domestic industry, more innovative monoclonal antibody products submitted investigational new drug (IND) application. At the same time, monoclonal antibody products from abroad which have been approved marketing authorization and/or conducted clinical trials submitted IND applications in China. The National Medical Products Administration (NMPA) issued the "Guideline of Investigational New Drug Application" (No. 16, 2018) which emphasized the chemical, manufacturing, and control (CMC) regulatory, and dossier requirements in IND application, greatly promoted the application quality of innovative biological products. However, compared to the Food and Drug Administration (FDA) and European Medicines Agency (EMA), our particular guidelines are insufficient, such as guideline on virus safety evaluation of biotechnological investigational medicinal products. This review investigated the questions raised by sponsors from 2018 to 2020, including the end of production cell (EOPC) and/or unprocessed bulk (UPB) testing and virus removal or inactivation validation. Meanwhile, sponsors submitted different dossiers due to differences in understanding of stage requirements of guidelines from domestic and abroad. Based on the guidelines of virus safety from NMPA, FDA, and EMA, and the technical considerations, this review puts forward personal suggestions on the adventitious agents testing and virus removal or inactivation validation in manufacturing process, aim to ensure virus safety of innovative monoclonal antibody products in clinical trials.

Cystatin Cystatin(CST)is a class of proteins that inhibit cysteine proteases and are widely distributed in human body fluid and secretion.The present study shows that the CST superfamily is closely related to the tumor,in which the cysteine protease inhibitor SN is the product expressed by the CST1 gene and is abnormal expression in various tumors.However,its occurrence and development of tumor as well as effects of invasion and metastasis on the specific mechanism is not yet clear.In this paper,we retrospectively analyze the related studies in recent years and review the progress of CST1 gene in tumor.

Sudden cardiac death (SC D ),m ostcom m only seen in coronary heart disease, is a kind of sud-den death caused by series of cardiac param eters, w hich usually com bines w ith m yocardial infarction. H ow ever, som e SC D s (including early m yocardial infarction) happen suddenly and cause death in a very short tim e. In these circum stances, typical m orphological changes are lack in m acroscopic or m icroscopic fields, w hich m ake such SC D s becom e the em phasis and difficulty in the present research. SC D caused by m yocardial infarction and abnorm alities of cardiac conduction system (C C S ) is related to atheroscle-rosis of coronary artery closely. T his paper review s cardiac dysfunction caused by m yocardial infarction and diseases of C C S from m orphology and m olecular biology, and explores potential relationship be-tw een them . T his paper aim s to provide clues to the m echanism of m yocardial infarction related sudden death and possible assistance for forensic diagnosis of SC D .

Objective To investigate the effects of rutaecarpine on the cerebral injury and calcitonin gene-related peptide (CGRP) level during focal cerebral ischemia-reperfusion injury in rats. Methods Rats were intravenously given three doses of rutaecarpine (50, 100 and 300?g/kg) or vehicle 30 minutes before experiment. Then the rats subjected middle cerebral artery occlusion (MCAO) for 2 hours, and neurological deficits scores were performed at 6, 12 and 24 hours after reperfusion. After the last test the animals were sacrificed, the infarct volumes were determined by TTC staining, and CGRP levels were measured by radioimmuoassay. Results Rutaecarpine significantly reduced infarct volume, and improved cerebral function in dose-dependent manner compared with vehicle. Rutaecarpine significantly inecreased brain CGRP levels after reperfusion as well. Conclusion Rutaecarpine has marked protective effects on ischemic brain damage in rats possibly by increasing CGRP release in the brain.

AIM: To study the processing principle of Fructus Schisandrae Sphenantherae steamed with wine. METHODS: The content of volatile oil was measured by means of volatile oil measuring method and identified by TLC. The total lignans was determined by spectrophotometry and the water-soluble extractive was measured by determination of extractive method. RESULTS: The volatile oil content in Fruetus Schisandrae Sphenantherae by steaming with wine decreased by 35.3%, TLC showed that a new spot was added; the lignans content had an increase of 28.9% and the water extractive was 2% less than in crude drug. CONCLUSION: Tranditional process is reasonable.

Objective To investigate the serum level of vascular endothelial growth factor (VEGF) in patients with coronary artery disease (CAD) and its clinical significance. Methods The study included 36 patients with CAD and 10 non-coronary heart disease patients as the control. The concentrations of serum VEGF in the coronary veins and the aorta were measured by an enzyme-linked immunsorbent assay (ELISA). Total mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation, and were cultured in M199 medium supplemented with 20% fetal bovine serum. After 7 days culture, attached cells were collected. The effect of VEGF on the migration of endothelial progenitor cells (EPCs) were assayed with modified Boyden chamber assay. Results The serum VEGF levels of stable angina pectoris (SAP) patients (208.46?44.74 ?g/L) and acute coronary syndrome (ACS) patients (267.29?93.99 ?g/L) were much higher than that of the controls group (120.77?26.25 ?g/L,P

Objective To assess clinical efficacy and safety of linezolid in treating postoperative intracranial infec-tion after neurosurgery operation,and provide clinical basis for the treatment of postoperative intracranial infection. Methods From January 2011 to December 2012,clinical data of 51 neurosurgery patients receiving linezolid for trea-ting postoperative intracranial infection were collected,changes in symptoms,temperature,cerebrospinal fluid (CSF)bacterial culture,as well as white blood cell (WBC)count,protein and glucose in CSF before and after therapy were compared,clinical efficacy and safety of linezolid in the treatment of postoperative intracranial infection were assessed based on the diagnostic criteria for intracranial infection.Results After linezolid treatment,30 cases of postoperative intracranial infection were cured,and linezolid was effective in 1 2 cases,improved in 5 cases,and ineffective in 4 cases,total effective rate was 92.16%.Of 47 cases with effective therapy,the mean therapy day were 12.5 d (2-27 d),11 of whom isolated gram-positive bacteria from CSF before therapy,and CSF culture were all negative after linezolid therapy.Conclusion Linezolid is effective for treating intracranial infection caused by Staphylococci,Enterococci and other gram-positive bacteria which failed to response to vancomycin therapy.

Objective To evaluate the removal effectiveness of air pollutants by photocatalytic nanocomposite coating. Methods TiO2-coated glass board was exposed to UV light of 365 nm in chamber with volume of 4 m3. The purification efficiencies of TiO2 nanocomposite coating for SO2?NO2 and formaldehyde were determined at light intensity of 107-271 ?W/cm2 and wind velocity of 0.3-1.5 m/s. Results The purification efficiencies of TiO2-coated glass board treated under this experimental condition for 30 min were 97%?90%, 69% for SO2?NO2 and formaldehyde in indoor air respectively, and only 48% for SO2 without exposure to UV light. Conclusion This method provided basic data for establishing an evaluation method for purification effectiveness of photocatalytic nanocomposite coating for indoor air pollutants. Further approach was needed for exploring the effects of various environmental influential factors on the purification effectiveness of TiO2 nanocomposite coating.